pycom13g07580
ERF Family

superfamily. Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007183
Physical Location & Seq
Forward (+)
5032567 .. 5033912
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g07580.2

Sequence Viewer

Length: 1083 bp
ATGGCTGCAACTGTTTCGCTCTCTTTCTCTTTAGATCCACAGCGGTACCACCACCACCGCCACTGCCATCTCTTCTCCGTCACCCACCACTTCAAACCCCACCAAAACCTTCCCGTCACTCCCTCTACCTCCTCAACTGTTCTCCCCAAGTACTCACTTTCTATTTTCCCCAAGTCAGACCGTTTCGCCTTCAAATCCTCCTCCTCCCCATCTCATTCTCCCACCACCACCACCGCCACCACCACAGCTGCTGATTCCACGACTACCGATTCCACCACCACCACTACTGATTCCGATACTACTACTTCAACAACTTCGTCATTTCTCGAATACCCTCTTAGAACAGGTAAGTTTCTGAGCAGTGAAGAGCTCGAGCAGCTCAAACGCCTCGAGGATTTCAGGTACTACCAGGAATTGGAATCTGGGTCGATGTGGGTTCGGGTGATGAGGCCGGAGGAGTTGGACGTCACCGTCGGGTTGCTCGCCGAGTCGTTCGCGGAGTCGATGCTTTTGCCTTCTGGGTATGTGTCTGTGCTGGGCTACTTGGTCAAGCAGTACTTGTTTGAAAGAATGGAGCTTCTTCCCAACACCGCCACGCTCATCGCGTTTTACAGAAGAAGAAAAGAAGAGGGCAAAGAAGAAGAAGAAGAACAACTGGAAGAAGTAGAAGAAGAAGATGAAGTTGAGCTTTTGGCGGGGACGGTGGAAGTTTGCTTTAACAAAAAGGGTGCCGTTGCCTCGCCTCCTACACCAACTCCTCCCAAGAATTCGCCTTATATTAGTAACATGGCGGTGAAAAAATCGCTTCGGAGGAGGGGCATCGGATGGCATCTTTTGAAGGCAAGCGAGGAACTAATCTCGCAGATGAGTTCCTCAAGAGAGGTGTACTTGCATTGCAGGATGATTGATACAGCTCCATTCAACATGTATAAAAAAGCAGGATACGACATTGTAGAGACGAATACCATTTTGGTTTTGTTGCTGTTGCAGAGGCGCAAGCACTTAATGTGCAAGAAACTTCCAGTGTTAACCAACTTTTCAGAATCGGATACATTCTGTTCTGAAGAGGAATTAACACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Acetyltransf_10 FR47

Protein Analysis

361

Amino Acids

40.9

Weight (kDa)

5.89

Isoelectric Point (pI)

59.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
AatII GACGTC 1 cut(s) 468
AbsI CCTCGAGG 1 cut(s) 389
Acc65I GGTACC 1 cut(s) 45
AccB1I GGYRCC 2 cut(s) 45, 728
AccB7I CCANNNNNTGG 1 cut(s) 415
AccII CGCG 2 cut(s) 497, 605
AciI CCGC 7 cut(s) 43, 58, 234, 497, 591, 695, 791
AclWI GGATC 1 cut(s) 29
AcsI RAATTY 1 cut(s) 766
AcuI CTGAAG 1 cut(s) 1083
AcyI GRCGYC 1 cut(s) 465
AfaI GTAC 5 cut(s) 47, 152, 404, 557, 887
AfiI CCNNNNNNNGG 1 cut(s) 415
AflIII ACRYGT 1 cut(s) 924
AgsI TTSAA 6 cut(s) 94, 193, 309, 566, 838, 922
AjnI CCWGG 1 cut(s) 408
AjuI GAANNNNNNNTTGG 2 cut(s) 953, 985
AluBI AGCT 6 cut(s) 248, 370, 379, 577, 688, 914
AluI AGCT 6 cut(s) 248, 370, 379, 577, 688, 914
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 1 cut(s) 950
AlwI GGATC 1 cut(s) 29
AlwNI CAGNNNCTG 1 cut(s) 251
Ama87I CYCGRG 2 cut(s) 371, 389
AoxI GGCC 1 cut(s) 449
ApeKI GCWGC 3 cut(s) 5, 248, 376
ApoI RAATTY 1 cut(s) 766
Asp718I GGTACC 1 cut(s) 45
AspLEI GCGC 1 cut(s) 996
AsuHPI GGTGA 4 cut(s) 73, 454, 460, 805
AvaI CYCGRG 2 cut(s) 371, 389
BaeI ACNNNNGTAYC 2 cut(s) 29, 62
BanI GGYRCC 2 cut(s) 45, 728
BanII GRGCYC 1 cut(s) 372
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 2 cut(s) 235, 388
BccI CCATC 3 cut(s) 75, 217, 819
BceAI ACGGC 1 cut(s) 716
BcgI CGANNNNNNTGC 3 cut(s) 31, 493, 527
BciT130I CCWGG 1 cut(s) 410
BciVI GTATCC 2 cut(s) 935, 1042
BcoDI GTCTC 1 cut(s) 950
BfuI GTATCC 2 cut(s) 935, 1042
BisI GCNGC 3 cut(s) 6, 249, 377
BlsI GCNGC 3 cut(s) 7, 250, 378
BmcAI AGTACT 2 cut(s) 152, 557
Bme1390I CCNGG 1 cut(s) 410
BmeT110I CYCGRG 2 cut(s) 371, 389
BmiI GGNNCC 2 cut(s) 47, 730
BmrFI CCNGG 1 cut(s) 410
BmsI GCATC 3 cut(s) 495, 828, 838
BpuEI CTTGAG 1 cut(s) 859
BsaHI GRCGYC 1 cut(s) 465
BsaXI ACNNNNNCTCC 2 cut(s) 739, 769
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 2 cut(s) 660, 1022
Bse3DI GCAATG 1 cut(s) 892
BseBI CCWGG 1 cut(s) 410
BseGI GGATG 2 cut(s) 830, 906
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMI GCAATG 1 cut(s) 892
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 2 cut(s) 660, 1022
BseRI GAGGAG 6 cut(s) 121, 190, 193, 470, 747, 826
BseXI GCAGC 2 cut(s) 235, 388
BseYI CCCAGC 1 cut(s) 535
Bsh1236I CGCG 2 cut(s) 497, 605
BshFI GGCC 1 cut(s) 451
BshNI GGYRCC 2 cut(s) 45, 728
BsiHKAI GWGCWC 1 cut(s) 372
BsiHKCI CYCGRG 2 cut(s) 371, 389
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 712
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 1 cut(s) 950
BsmBI CGTCTC 1 cut(s) 950
BsmFI GGGAC 1 cut(s) 712
BsnI GGCC 1 cut(s) 451
BsoBI CYCGRG 2 cut(s) 371, 389
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 1 cut(s) 34
BspACI CCGC 7 cut(s) 43, 58, 234, 497, 591, 695, 791
BspANI GGCC 1 cut(s) 451
BspCNI CTCAG 1 cut(s) 348
BspFNI CGCG 2 cut(s) 497, 605
BspLI GGNNCC 2 cut(s) 47, 730
BspPI GGATC 1 cut(s) 29
BspQI GCTCTTC 1 cut(s) 360
BspT107I GGYRCC 2 cut(s) 45, 728
BsrDI GCAATG 1 cut(s) 892
BsrI ACTGG 2 cut(s) 660, 1022
BssMI GATC 1 cut(s) 34
BssNI GRCGYC 1 cut(s) 465
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 5 cut(s) 13, 139, 182, 472, 703
Bst6I CTCTTC 4 cut(s) 77, 360, 621, 1059
BstACI GRCGYC 1 cut(s) 465
BstC8I GCNNGC 3 cut(s) 483, 844, 998
BstDEI CTNAG 2 cut(s) 338, 356
BstF5I GGATG 2 cut(s) 830, 906
BstFNI CGCG 2 cut(s) 497, 605
BstHHI GCGC 1 cut(s) 996
BstKTI GATC 1 cut(s) 37
BstMAI GTCTC 1 cut(s) 950
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 1 cut(s) 376
BstNI CCWGG 1 cut(s) 410
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 1 cut(s) 408
BstUI CGCG 2 cut(s) 497, 605
BstV1I GCAGC 2 cut(s) 235, 388
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuI GTATCC 2 cut(s) 935, 1042
BsuRI GGCC 1 cut(s) 451
BtgZI GCGATG 1 cut(s) 586
BtsCI GGATG 2 cut(s) 830, 906
BtsI GCAGTG 2 cut(s) 61, 367
BtsIMutI CAGTG 3 cut(s) 61, 367, 1029
Cac8I GCNNGC 3 cut(s) 483, 844, 998
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 1 cut(s) 996
Csp6I GTAC 5 cut(s) 46, 151, 403, 556, 886
CviAII CATG 2 cut(s) 787, 925
CviJI RGCY 9 cut(s) 5, 248, 370, 379, 451, 540, 577, 688, 914
CviKI_1 RGCY 9 cut(s) 5, 248, 370, 379, 451, 540, 577, 688, 914
CviQI GTAC 5 cut(s) 46, 151, 403, 556, 886
DdeI CTNAG 2 cut(s) 338, 356
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
Eam1104I CTCTTC 4 cut(s) 77, 360, 621, 1059
EarI CTCTTC 4 cut(s) 77, 360, 621, 1059
Ecl136II GAGCTC 1 cut(s) 370
Eco24I GRGCYC 1 cut(s) 372
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 1083
Eco88I CYCGRG 2 cut(s) 371, 389
EcoICRI GAGCTC 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 766
EcoRII CCWGG 1 cut(s) 408
EcoT38I GRGCYC 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 950
FaeI CATG 2 cut(s) 790, 928
FaiI YATR 5 cut(s) 525, 777, 788, 926, 930
FalI AAGNNNNNCTT 4 cut(s) 542, 574, 672, 704
FaqI GGGAC 1 cut(s) 712
FatI CATG 2 cut(s) 786, 924
FauI CCCGC 1 cut(s) 688
Fnu4HI GCNGC 3 cut(s) 6, 249, 377
FokI GGATG 2 cut(s) 837, 913
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 3 cut(s) 6, 249, 377
GlaI GCGC 1 cut(s) 995
GluI GCNGC 3 cut(s) 6, 249, 377
GsaI CCCAGC 1 cut(s) 539
HaeIII GGCC 1 cut(s) 451
HapII CCGG 1 cut(s) 452
HhaI GCGC 1 cut(s) 996
Hin1I GRCGYC 1 cut(s) 465
Hin1II CATG 2 cut(s) 790, 928
Hin6I GCGC 1 cut(s) 994
HinP1I GCGC 1 cut(s) 994
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HinfI GANTC 7 cut(s) 254, 269, 290, 419, 488, 500, 1043
HpaI GTTAAC 1 cut(s) 1029
HpaII CCGG 1 cut(s) 452
HphI GGTGA 4 cut(s) 73, 454, 460, 805
Hpy166II GTNNAC 2 cut(s) 886, 1029
Hpy188I TCNGA 8 cut(s) 178, 295, 357, 810, 824, 1042, 1048, 1063
Hpy188III TCNNGA 2 cut(s) 326, 876
Hpy8I GTNNAC 2 cut(s) 886, 1029
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 4 cut(s) 119, 199, 525, 832
HpyCH4III ACNGT 5 cut(s) 13, 139, 182, 472, 703
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 5 cut(s) 8, 892, 897, 988, 1011
HpyF10VI GCNNNNNNNGC 1 cut(s) 376
HpyF3I CTNAG 2 cut(s) 338, 356
HpySE526I ACGT 1 cut(s) 465
Hsp92I GRCGYC 1 cut(s) 465
Hsp92II CATG 2 cut(s) 790, 928
HspAI GCGC 1 cut(s) 994
KpnI GGTACC 1 cut(s) 49
KspAI GTTAAC 1 cut(s) 1029
Kzo9I GATC 1 cut(s) 34
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 2 cut(s) 574, 919
Lsp1109I GCAGC 2 cut(s) 235, 388
LweI GCATC 3 cut(s) 495, 828, 838
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 4 cut(s) 79, 115, 466, 782
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 372
MluCI AATT 3 cut(s) 413, 766, 1070
MlyI GAGTC 2 cut(s) 497, 509
MmeI TCCRAC 1 cut(s) 441
MseI TTAA 4 cut(s) 717, 1004, 1028, 1073
MslI CAYNNNNRTG 1 cut(s) 791
MspA1I CMGCKG 2 cut(s) 43, 248
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 1 cut(s) 410
MvaI CCWGG 1 cut(s) 410
MvnI CGCG 2 cut(s) 497, 605
MwoI GCNNNNNNNGC 1 cut(s) 376
NdeII GATC 1 cut(s) 34
NlaIII CATG 2 cut(s) 790, 928
NlaIV GGNNCC 2 cut(s) 47, 730
NmeAIII GCCGAG 1 cut(s) 511
NmuCI GTSAC 3 cut(s) 79, 115, 466
NspI RCATGY 1 cut(s) 928
PaeR7I CTCGAG 2 cut(s) 371, 389
PciI ACATGT 1 cut(s) 924
PciSI GCTCTTC 1 cut(s) 360
PcsI WCGNNNNNNNCGW 1 cut(s) 602
PfeI GAWTC 5 cut(s) 254, 269, 290, 419, 1043
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 3 cut(s) 7, 250, 378
PleI GAGTC 2 cut(s) 496, 508
PpsI GAGTC 2 cut(s) 496, 508
PscI ACATGT 1 cut(s) 924
Psp124BI GAGCTC 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 408
PspFI CCCAGC 1 cut(s) 535
PspGI CCWGG 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 47, 730
PspXI VCTCGAGB 2 cut(s) 371, 389
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 5 cut(s) 47, 152, 404, 557, 887
RsaNI GTAC 5 cut(s) 46, 151, 403, 556, 886
RseI CAYNNNNRTG 1 cut(s) 791
SacI GAGCTC 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 4 cut(s) 717, 1004, 1028, 1073
SatI GCNGC 3 cut(s) 6, 249, 377
Sau3AI GATC 1 cut(s) 34
ScaI AGTACT 2 cut(s) 152, 557
SchI GAGTC 2 cut(s) 497, 509
ScrFI CCNGG 1 cut(s) 410
SduI GDGCHC 1 cut(s) 372
SfaNI GCATC 3 cut(s) 495, 828, 838
Sfr274I CTCGAG 2 cut(s) 371, 389
SlaI CTCGAG 2 cut(s) 371, 389
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 3 cut(s) 371, 389, 874
SmoI CTYRAG 3 cut(s) 371, 389, 874
Sse9I AATT 3 cut(s) 413, 766, 1070
SsiI CCGC 7 cut(s) 43, 58, 234, 497, 591, 695, 791
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 408
TaaI ACNGT 5 cut(s) 13, 139, 182, 472, 703
TaiI ACGT 1 cut(s) 468
TaqI TCGA 5 cut(s) 327, 372, 390, 428, 503
TasI AATT 3 cut(s) 413, 766, 1070
TatI WGTACW 3 cut(s) 150, 555, 885
TfiI GAWTC 5 cut(s) 254, 269, 290, 419, 1043
Tru1I TTAA 4 cut(s) 717, 1004, 1028, 1073
Tru9I TTAA 4 cut(s) 717, 1004, 1028, 1073
TscAI CASTG 3 cut(s) 68, 367, 1029
TseFI GTSAC 3 cut(s) 79, 115, 466
TseI GCWGC 3 cut(s) 5, 248, 376
Tsp45I GTSAC 3 cut(s) 79, 115, 466
TspDTI ATGAA 1 cut(s) 693
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 3 cut(s) 68, 367, 1029
Van91I CCANNNNNTGG 1 cut(s) 415
XapI RAATTY 1 cut(s) 766
XceI RCATGY 1 cut(s) 928
XhoI CTCGAG 2 cut(s) 371, 389
ZraI GACGTC 1 cut(s) 466
ZrmI AGTACT 2 cut(s) 152, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.