MD16G1136300.v1.1

dna binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009933
Physical Location & Seq
Forward (+)
10439176 .. 10439889
714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1136300.v1.1.491

Sequence Viewer

Length: 714 bp
ATGAGGCGAGACGAACCAGAACTCGAGAGGCGACTCTGCGGCGTCTGCGGCTTCTCCGAACGGTGGTTCCTCCACTACATCCGTCACAAAGGGGCGTTTCAGAGGCTCTGCACCAACTGCGTCCTCCAAAACTACCGAGACCTCTACTGCCCTCTCTGTCTCGACTTCTTCGACCAACCGCTTCCGGCACGTGACCGTGTCATGTGCGTCAAGTGCCCTTCCGTTTCTCACTCCGCCTGCGTCCTCGTCGCTAATTCCTCCTTCCATGGCTTCGAGTGCCGTACCTGCCCACAGGGCTACCCCTTCTTCACCCTCAATCGCCCCCAAAACGACGCCAACGACGCCAAAACGGCATTCGTCATCGACAAGGCCAACGCCAAGGCCCTCGTCGCCGCCGCCAAAATTGCGGCGGCTTCAATGGCGAAAGCCGCCATCTTGGCCAGGACCGACTCCGAGCGCCGCGTCAGGGAGGCCGTGTTGGCGAAGAAGAAGGCGAGGGAGGCATTGGAGAAACTCAGTTCTCTTGTTAACAAGCAGAAGCAGCAGGATTCGAAAGCTGGAGATGTTTCGGGGCCTGAGAGCCTCAACGGGAAGCCGAAATTGGAGAGTTCTGGTGGAATTCCAGCGGCGCCCAAGATTGTTAAGGCTGAGGGAAGTAATGGGTTGGTTGGTGATCGATTAAAAATCAGTAATGTTGACGCCATGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

26.08

Weight (kDa)

9.12

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 628
AccII CGCG 1 cut(s) 462
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 1 cut(s) 618
AcvI CACGTG 1 cut(s) 191
AcyI GRCGYC 5 cut(s) 42, 333, 342, 629, 699
AfaI GTAC 1 cut(s) 283
AfiI CCNNNNNNNGG 2 cut(s) 63, 466
AgsI TTSAA 1 cut(s) 417
AjnI CCWGG 1 cut(s) 440
AjuI GAANNNNNNNTTGG 4 cut(s) 338, 370, 584, 616
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
AluBI AGCT 1 cut(s) 557
AluI AGCT 1 cut(s) 557
Alw26I GTCTC 3 cut(s) 3, 132, 164
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 5 cut(s) 369, 381, 438, 471, 572
ApeKI GCWGC 1 cut(s) 541
ApoI RAATTY 1 cut(s) 618
AspLEI GCGC 2 cut(s) 459, 631
AspS9I GGNCC 3 cut(s) 382, 444, 572
AsuHPI GGTGA 2 cut(s) 301, 683
AsuII TTCGAA 1 cut(s) 551
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 1 cut(s) 444
BaeGI GKGCMC 1 cut(s) 218
BalI TGGCCA 1 cut(s) 440
BanI GGYRCC 1 cut(s) 628
BbrPI CACGTG 1 cut(s) 191
BbvCI CCTCAGC 1 cut(s) 648
BbvI GCAGC 1 cut(s) 553
BccI CCATC 1 cut(s) 440
BceAI ACGGC 3 cut(s) 264, 366, 458
BciT130I CCWGG 1 cut(s) 442
BcoDI GTCTC 3 cut(s) 3, 132, 164
BfoI RGCGCY 2 cut(s) 460, 632
BfuAI ACCTGC 1 cut(s) 293
BglI GCCNNNNNGGC 4 cut(s) 294, 350, 437, 479
Bme1390I CCNGG 1 cut(s) 442
Bme18I GGWCC 1 cut(s) 444
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 3 cut(s) 382, 444, 572
BmiI GGNNCC 3 cut(s) 68, 573, 630
BmrFI CCNGG 1 cut(s) 442
BpmI CTGGAG 1 cut(s) 579
Bpu10I CCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 1 cut(s) 551
Bsa29I ATCGAT 1 cut(s) 676
BsaAI YACGTR 1 cut(s) 191
BsaHI GRCGYC 5 cut(s) 42, 333, 342, 629, 699
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 3 cut(s) 265, 378, 702
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 466
BseBI CCWGG 1 cut(s) 442
BseCI ATCGAT 1 cut(s) 676
BseDI CCNNGG 3 cut(s) 265, 378, 702
BseGI GGATG 1 cut(s) 78
BseLI CCNNNNNNNGG 2 cut(s) 63, 466
BseMII CTCAG 3 cut(s) 529, 567, 639
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 1 cut(s) 553
BsgI GTGCAG 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 5 cut(s) 371, 383, 440, 473, 574
BshNI GGYRCC 1 cut(s) 628
BshVI ATCGAT 1 cut(s) 676
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 1 cut(s) 185
BslI CCNNNNNNNGG 2 cut(s) 63, 466
BsmAI GTCTC 3 cut(s) 3, 132, 164
BsmBI CGTCTC 1 cut(s) 3
BsmI GAATGC 1 cut(s) 353
BsnI GGCC 5 cut(s) 371, 383, 440, 473, 574
Bso31I GGTCTC 1 cut(s) 132
BsoBI CYCGRG 1 cut(s) 23
Bsp119I TTCGAA 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 218
Bsp143I GATC 1 cut(s) 673
Bsp19I CCATGG 2 cut(s) 265, 702
BspANI GGCC 5 cut(s) 371, 383, 440, 473, 574
BspCNI CTCAG 3 cut(s) 528, 568, 640
BspDI ATCGAT 1 cut(s) 676
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 3 cut(s) 68, 573, 630
BspMI ACCTGC 1 cut(s) 293
BspT104I TTCGAA 1 cut(s) 551
BspT107I GGYRCC 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 132
BssECI CCNNGG 3 cut(s) 265, 378, 702
BssMI GATC 1 cut(s) 673
BssNI GRCGYC 5 cut(s) 42, 333, 342, 629, 699
BssT1I CCWWGG 3 cut(s) 265, 378, 702
Bst2UI CCWGG 1 cut(s) 442
Bst4CI ACNGT 2 cut(s) 63, 197
BstACI GRCGYC 5 cut(s) 42, 333, 342, 629, 699
BstAPI GCANNNNNTGC 1 cut(s) 117
BstBAI YACGTR 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 551
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 3 cut(s) 515, 576, 648
BstDSI CCRYGG 2 cut(s) 265, 702
BstF5I GGATG 1 cut(s) 78
BstFNI CGCG 1 cut(s) 462
BstH2I RGCGCY 2 cut(s) 460, 632
BstHHI GCGC 2 cut(s) 459, 631
BstKTI GATC 1 cut(s) 676
BstMAI GTCTC 3 cut(s) 3, 132, 164
BstMBI GATC 1 cut(s) 673
BstNI CCWGG 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 440
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 553
Bsu15I ATCGAT 1 cut(s) 676
BsuRI GGCC 5 cut(s) 371, 383, 440, 473, 574
BsuTUI ATCGAT 1 cut(s) 676
BtgI CCRYGG 2 cut(s) 265, 702
BtsCI GGATG 1 cut(s) 78
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 1 cut(s) 238
CfoI GCGC 2 cut(s) 459, 631
Cfr13I GGNCC 3 cut(s) 382, 444, 572
ClaI ATCGAT 1 cut(s) 676
CseI GACGC 8 cut(s) 31, 109, 196, 229, 341, 350, 451, 707
Csp6I GTAC 1 cut(s) 282
CviAII CATG 3 cut(s) 202, 266, 703
CviQI GTAC 1 cut(s) 282
DdeI CTNAG 3 cut(s) 515, 576, 648
DinI GGCGCC 1 cut(s) 630
DpnI GATC 1 cut(s) 675
DpnII GATC 1 cut(s) 673
EaeI YGGCCR 1 cut(s) 438
EciI GGCGGA 1 cut(s) 223
Eco130I CCWWGG 3 cut(s) 265, 378, 702
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 444
Eco72I CACGTG 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 23
EcoO109I RGGNCCY 2 cut(s) 382, 572
EcoRI GAATTC 1 cut(s) 618
EcoRII CCWGG 1 cut(s) 440
EcoT14I CCWWGG 3 cut(s) 265, 378, 702
EgeI GGCGCC 1 cut(s) 630
EheI GGCGCC 1 cut(s) 630
ErhI CCWWGG 3 cut(s) 265, 378, 702
Esp3I CGTCTC 1 cut(s) 3
FaeI CATG 3 cut(s) 205, 269, 706
FaiI YATR 3 cut(s) 203, 267, 704
FatI CATG 3 cut(s) 201, 265, 702
FokI GGATG 1 cut(s) 65
GlaI GCGC 2 cut(s) 458, 630
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 2 cut(s) 460, 632
HaeIII GGCC 5 cut(s) 371, 383, 440, 473, 574
HapII CCGG 1 cut(s) 185
HgaI GACGC 8 cut(s) 31, 109, 196, 229, 341, 350, 451, 707
HhaI GCGC 2 cut(s) 459, 631
Hin1I GRCGYC 5 cut(s) 42, 333, 342, 629, 699
Hin1II CATG 3 cut(s) 205, 269, 706
Hin6I GCGC 2 cut(s) 457, 629
HinP1I GCGC 2 cut(s) 457, 629
HincII GTYRAC 2 cut(s) 529, 697
HindII GTYRAC 2 cut(s) 529, 697
HinfI GANTC 3 cut(s) 33, 449, 548
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 1 cut(s) 185
HphI GGTGA 2 cut(s) 301, 683
Hpy166II GTNNAC 2 cut(s) 529, 697
Hpy188I TCNGA 3 cut(s) 58, 102, 454
Hpy188III TCNNGA 2 cut(s) 25, 161
Hpy8I GTNNAC 2 cut(s) 529, 697
Hpy99I CGWCG 4 cut(s) 251, 335, 344, 392
HpyAV CCTTC 4 cut(s) 228, 271, 313, 484
HpyCH4III ACNGT 2 cut(s) 63, 197
HpyCH4IV ACGT 1 cut(s) 190
HpyCH4V TGCA 1 cut(s) 111
HpyF3I CTNAG 3 cut(s) 515, 576, 648
HpySE526I ACGT 1 cut(s) 190
Hsp92I GRCGYC 5 cut(s) 42, 333, 342, 629, 699
Hsp92II CATG 3 cut(s) 205, 269, 706
HspAI GCGC 2 cut(s) 457, 629
KasI GGCGCC 1 cut(s) 628
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 1 cut(s) 673
Lsp1109I GCAGC 1 cut(s) 553
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 2 cut(s) 83, 191
MalI GATC 1 cut(s) 675
MboI GATC 1 cut(s) 673
MboII GAAGA 4 cut(s) 160, 298, 496, 499
MhlI GDGCHC 1 cut(s) 218
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 4 cut(s) 253, 402, 599, 618
MluNI TGGCCA 1 cut(s) 440
Mly113I GGCGCC 1 cut(s) 629
MlyI GAGTC 2 cut(s) 27, 443
Mox20I TGGCCA 1 cut(s) 440
MscI TGGCCA 1 cut(s) 440
MseI TTAA 3 cut(s) 528, 642, 680
Msp20I TGGCCA 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 1 cut(s) 442
Mva1269I GAATGC 1 cut(s) 353
MvaI CCWGG 1 cut(s) 442
MvnI CGCG 1 cut(s) 462
NarI GGCGCC 1 cut(s) 629
NcoI CCATGG 2 cut(s) 265, 702
NdeII GATC 1 cut(s) 673
NlaIII CATG 3 cut(s) 205, 269, 706
NlaIV GGNNCC 3 cut(s) 68, 573, 630
NmuCI GTSAC 2 cut(s) 83, 191
NspV TTCGAA 1 cut(s) 551
PaeR7I CTCGAG 1 cut(s) 23
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 336
PctI GAATGC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 548
PflFI GACNNNGTC 1 cut(s) 197
PleI GAGTC 2 cut(s) 27, 443
PluTI GGCGCC 1 cut(s) 632
PmaCI CACGTG 1 cut(s) 191
PmlI CACGTG 1 cut(s) 191
PpsI GAGTC 2 cut(s) 27, 443
Ppu21I YACGTR 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 440
PspCI CACGTG 1 cut(s) 191
PspGI CCWGG 1 cut(s) 440
PspN4I GGNNCC 3 cut(s) 68, 573, 630
PspPI GGNCC 3 cut(s) 382, 444, 572
PsyI GACNNNGTC 1 cut(s) 197
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
SaqAI TTAA 3 cut(s) 528, 642, 680
Sau3AI GATC 1 cut(s) 673
Sau96I GGNCC 3 cut(s) 382, 444, 572
SchI GAGTC 2 cut(s) 27, 443
ScrFI CCNGG 1 cut(s) 442
SduI GDGCHC 1 cut(s) 218
SetI ASST 4 cut(s) 144, 193, 287, 559
SfoI GGCGCC 1 cut(s) 630
Sfr274I CTCGAG 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 551
SinI GGWCC 1 cut(s) 444
SlaI CTCGAG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 4 cut(s) 253, 402, 599, 618
SspDI GGCGCC 1 cut(s) 628
StyD4I CCNGG 1 cut(s) 440
StyI CCWWGG 3 cut(s) 265, 378, 702
TaaI ACNGT 2 cut(s) 63, 197
TaiI ACGT 1 cut(s) 193
TaqI TCGA 7 cut(s) 24, 162, 171, 273, 363, 551, 676
TaqII GACCGA 1 cut(s) 461
TasI AATT 4 cut(s) 253, 402, 599, 618
TauI GCSGC 9 cut(s) 42, 51, 395, 398, 410, 413, 431, 462, 629
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 3 cut(s) 528, 642, 680
Tru9I TTAA 3 cut(s) 528, 642, 680
TseFI GTSAC 2 cut(s) 83, 191
TseI GCWGC 1 cut(s) 541
Tsp45I GTSAC 2 cut(s) 83, 191
TspGWI ACGGA 2 cut(s) 71, 211
Tth111I GACNNNGTC 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 444
XapI RAATTY 1 cut(s) 618
XhoI CTCGAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.