pycom16g11990

dna binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009933
Physical Location & Seq
Forward (+)
8384423 .. 8385145
723 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g11990.1

Sequence Viewer

Length: 723 bp
ATGGCAATGAGGCGAGACGAACCCGAACTTGAGAGGCGACTCTGCGGCGTCTGCGGCTCCTCCGAACGGTGGTTCCTCCACTACATCCGCCACAAAGGGGCGTTTCAGAGGCTCTGCACCAACTGCGTCCTCCAAAACTACCGAGGCCTCTACTGCCCTCTCTGTCTCGACTACTTGGACCAACCCCTACCGGCAAGTGACCGGGTCATGTGCGTCAAGTGCCCTTCCGTTTCTCACCCCGCCTGCGTCCTCATCGCTAATTCCTCCTTCCATGGCTTCGAGTGCCATACCTGCGCAGAGGGCTACCCCTTCTTCACCCTCAATCGCCCCCAAAACGATGCCAACGACGCCAAAACGGCATGTCTCGTCGACAAGGCCAACGCCAAGGCCCTCGTCGCCGCCGCCAAAATTGCGGCGGCTTCAATGGCGAAAGCCGCCGTCTTGGCCAGGACCGACTCCGAGCGCCGCGTCAGGGAGGCCGTGTTGGCGAAGAAGAAGGCGAGGGAGGCATTGGAGAAACTCAGTTCTCTGGTTAACAAGCAGAAGCAGCAGGATTCGAAGGCTGGAGATGTTGTTTCGGGGCCTGAGAGCCTCAACGGGAAACCGAAATTAGAGAGTTCTGATGGAATTCCAGCGGCGCCCAAGATTGTTAAGGCTGAGGGAAGTAATGGGTTGGTTGGTGATCGATTAAAAATGGGAAATGTTGACGCCATGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

26.14

Weight (kDa)

8.79

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 295
Acc36I ACCTGC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 637
AccI GTMKAC 1 cut(s) 369
AccII CGCG 1 cut(s) 468
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 1 cut(s) 627
AcyI GRCGYC 4 cut(s) 48, 348, 638, 708
AfiI CCNNNNNNNGG 4 cut(s) 66, 69, 97, 472
AgsI TTSAA 1 cut(s) 423
AjnI CCWGG 1 cut(s) 446
AloI GAACNNNNNNTCC 2 cut(s) 57, 89
Alw26I GTCTC 3 cut(s) 9, 170, 368
AoxI GGCC 6 cut(s) 145, 375, 387, 444, 477, 581
ApeKI GCWGC 1 cut(s) 547
ApoI RAATTY 1 cut(s) 627
AspLEI GCGC 3 cut(s) 296, 465, 640
AspS9I GGNCC 4 cut(s) 178, 388, 450, 581
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 3 cut(s) 227, 307, 692
AsuII TTCGAA 1 cut(s) 557
AvaII GGWCC 2 cut(s) 178, 450
BaeGI GKGCMC 1 cut(s) 224
BalI TGGCCA 1 cut(s) 446
BanI GGYRCC 1 cut(s) 637
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 1 cut(s) 559
BccI CCATC 1 cut(s) 617
BceAI ACGGC 3 cut(s) 372, 422, 464
BciT130I CCWGG 1 cut(s) 448
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 3 cut(s) 9, 170, 368
BfoI RGCGCY 2 cut(s) 466, 641
BfuAI ACCTGC 1 cut(s) 299
BglI GCCNNNNNGGC 3 cut(s) 356, 443, 485
Bme1390I CCNGG 2 cut(s) 203, 448
Bme18I GGWCC 2 cut(s) 178, 450
BmgT120I GGNCC 4 cut(s) 178, 388, 450, 581
BmiI GGNNCC 4 cut(s) 58, 74, 582, 639
BmrFI CCNGG 2 cut(s) 203, 448
BmsI GCATC 1 cut(s) 328
BpmI CTGGAG 1 cut(s) 585
Bpu10I CCTNAGC 1 cut(s) 657
Bpu14I TTCGAA 1 cut(s) 557
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 203
Bsa29I ATCGAT 1 cut(s) 685
BsaHI GRCGYC 4 cut(s) 48, 348, 638, 708
BsaJI CCNNGG 4 cut(s) 142, 271, 384, 711
BsaXI ACNNNNNCTCC 4 cut(s) 467, 497, 558, 588
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 69, 97, 472
Bse118I RCCGGY 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 448
BseCI ATCGAT 1 cut(s) 685
BseDI CCNNGG 4 cut(s) 142, 271, 384, 711
BseGI GGATG 1 cut(s) 84
BseLI CCNNNNNNNGG 4 cut(s) 66, 69, 97, 472
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 535, 576, 648
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 1 cut(s) 224
BseXI GCAGC 1 cut(s) 559
BsgI GTGCAG 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 468
BshFI GGCC 6 cut(s) 147, 377, 389, 446, 479, 583
BshNI GGYRCC 1 cut(s) 637
BshVI ATCGAT 1 cut(s) 685
BsiSI CCGG 2 cut(s) 191, 202
BslI CCNNNNNNNGG 4 cut(s) 66, 69, 97, 472
BsmAI GTCTC 3 cut(s) 9, 170, 368
BsmBI CGTCTC 1 cut(s) 9
BsnI GGCC 6 cut(s) 147, 377, 389, 446, 479, 583
Bsp119I TTCGAA 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 1 cut(s) 682
Bsp19I CCATGG 2 cut(s) 271, 711
BspANI GGCC 6 cut(s) 147, 377, 389, 446, 479, 583
BspCNI CTCAG 3 cut(s) 534, 577, 649
BspDI ATCGAT 1 cut(s) 685
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 4 cut(s) 58, 74, 582, 639
BspMI ACCTGC 1 cut(s) 299
BspT104I TTCGAA 1 cut(s) 557
BspT107I GGYRCC 1 cut(s) 637
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 190
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 4 cut(s) 142, 271, 384, 711
BssMI GATC 1 cut(s) 682
BssNI GRCGYC 4 cut(s) 48, 348, 638, 708
BssT1I CCWWGG 3 cut(s) 271, 384, 711
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 1 cut(s) 69
BstACI GRCGYC 4 cut(s) 48, 348, 638, 708
BstAPI GCANNNNNTGC 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 557
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 3 cut(s) 521, 585, 657
BstDSI CCRYGG 2 cut(s) 271, 711
BstF5I GGATG 1 cut(s) 84
BstFNI CGCG 1 cut(s) 468
BstH2I RGCGCY 2 cut(s) 466, 641
BstHHI GCGC 3 cut(s) 296, 465, 640
BstKTI GATC 1 cut(s) 685
BstMAI GTCTC 3 cut(s) 9, 170, 368
BstMBI GATC 1 cut(s) 682
BstNI CCWGG 1 cut(s) 448
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 2 cut(s) 201, 446
BstSLI GKGCMC 1 cut(s) 224
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 1 cut(s) 559
Bsu15I ATCGAT 1 cut(s) 685
BsuRI GGCC 6 cut(s) 147, 377, 389, 446, 479, 583
BsuTUI ATCGAT 1 cut(s) 685
BtgI CCRYGG 2 cut(s) 271, 711
BtgZI GCGATG 1 cut(s) 238
BtsCI GGATG 1 cut(s) 84
BveI ACCTGC 1 cut(s) 299
Cac8I GCNNGC 1 cut(s) 244
CfoI GCGC 3 cut(s) 296, 465, 640
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 4 cut(s) 178, 388, 450, 581
ClaI ATCGAT 1 cut(s) 685
CseI GACGC 7 cut(s) 37, 115, 202, 235, 356, 457, 716
CviAII CATG 4 cut(s) 208, 272, 360, 712
DdeI CTNAG 3 cut(s) 521, 585, 657
DinI GGCGCC 1 cut(s) 639
DpnI GATC 1 cut(s) 684
DpnII GATC 1 cut(s) 682
EaeI YGGCCR 1 cut(s) 444
EciI GGCGGA 1 cut(s) 77
Eco130I CCWWGG 3 cut(s) 271, 384, 711
Eco147I AGGCCT 1 cut(s) 147
Eco47I GGWCC 2 cut(s) 178, 450
EcoO109I RGGNCCY 2 cut(s) 388, 581
EcoRI GAATTC 1 cut(s) 627
EcoRII CCWGG 1 cut(s) 446
EcoT14I CCWWGG 3 cut(s) 271, 384, 711
EgeI GGCGCC 1 cut(s) 639
EheI GGCGCC 1 cut(s) 639
ErhI CCWWGG 3 cut(s) 271, 384, 711
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 4 cut(s) 211, 275, 363, 715
FaiI YATR 5 cut(s) 209, 273, 288, 361, 713
FatI CATG 4 cut(s) 207, 271, 359, 711
FauI CCCGC 1 cut(s) 247
FblI GTMKAC 1 cut(s) 369
FokI GGATG 1 cut(s) 71
FspI TGCGCA 1 cut(s) 295
GlaI GCGC 3 cut(s) 295, 464, 639
GsuI CTGGAG 1 cut(s) 585
HaeII RGCGCY 2 cut(s) 466, 641
HaeIII GGCC 6 cut(s) 147, 377, 389, 446, 479, 583
HapII CCGG 2 cut(s) 191, 202
HgaI GACGC 7 cut(s) 37, 115, 202, 235, 356, 457, 716
HhaI GCGC 3 cut(s) 296, 465, 640
Hin1I GRCGYC 4 cut(s) 48, 348, 638, 708
Hin1II CATG 4 cut(s) 211, 275, 363, 715
Hin6I GCGC 3 cut(s) 294, 463, 638
HinP1I GCGC 3 cut(s) 294, 463, 638
HincII GTYRAC 3 cut(s) 370, 535, 706
HindII GTYRAC 3 cut(s) 370, 535, 706
HinfI GANTC 3 cut(s) 39, 455, 554
HpaI GTTAAC 1 cut(s) 535
HpaII CCGG 2 cut(s) 191, 202
HphI GGTGA 3 cut(s) 227, 307, 692
Hpy166II GTNNAC 3 cut(s) 370, 535, 706
Hpy188I TCNGA 4 cut(s) 64, 108, 460, 622
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 3 cut(s) 370, 535, 706
Hpy99I CGWCG 3 cut(s) 350, 371, 398
HpyAV CCTTC 5 cut(s) 234, 277, 319, 490, 553
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4V TGCA 1 cut(s) 117
HpyF3I CTNAG 3 cut(s) 521, 585, 657
Hsp92I GRCGYC 4 cut(s) 48, 348, 638, 708
Hsp92II CATG 4 cut(s) 211, 275, 363, 715
HspAI GCGC 3 cut(s) 294, 463, 638
KasI GGCGCC 1 cut(s) 637
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 1 cut(s) 682
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 1 cut(s) 328
MaeIII GTNAC 1 cut(s) 197
MalI GATC 1 cut(s) 684
MboI GATC 1 cut(s) 682
MboII GAAGA 3 cut(s) 304, 502, 505
MhlI GDGCHC 1 cut(s) 224
MlsI TGGCCA 1 cut(s) 446
MluCI AATT 4 cut(s) 259, 408, 608, 627
MluNI TGGCCA 1 cut(s) 446
Mly113I GGCGCC 1 cut(s) 638
MlyI GAGTC 2 cut(s) 33, 449
Mox20I TGGCCA 1 cut(s) 446
MscI TGGCCA 1 cut(s) 446
MseI TTAA 3 cut(s) 534, 651, 689
Msp20I TGGCCA 1 cut(s) 446
MspA1I CMGCKG 1 cut(s) 635
MspI CCGG 2 cut(s) 191, 202
MspR9I CCNGG 2 cut(s) 203, 448
MvaI CCWGG 1 cut(s) 448
MvnI CGCG 1 cut(s) 468
NarI GGCGCC 1 cut(s) 638
NciI CCSGG 1 cut(s) 203
NcoI CCATGG 2 cut(s) 271, 711
NdeII GATC 1 cut(s) 682
NlaIII CATG 4 cut(s) 211, 275, 363, 715
NlaIV GGNNCC 4 cut(s) 58, 74, 582, 639
NmuCI GTSAC 1 cut(s) 197
NsbI TGCGCA 1 cut(s) 295
NspI RCATGY 1 cut(s) 363
NspV TTCGAA 1 cut(s) 557
PceI AGGCCT 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 1 cut(s) 554
PflFI GACNNNGTC 1 cut(s) 203
PleI GAGTC 2 cut(s) 33, 449
PluTI GGCGCC 1 cut(s) 641
PpsI GAGTC 2 cut(s) 33, 449
Psp6I CCWGG 1 cut(s) 446
PspGI CCWGG 1 cut(s) 446
PspN4I GGNNCC 4 cut(s) 58, 74, 582, 639
PspPI GGNCC 4 cut(s) 178, 388, 450, 581
PsyI GACNNNGTC 1 cut(s) 203
SalI GTCGAC 1 cut(s) 368
SaqAI TTAA 3 cut(s) 534, 651, 689
Sau3AI GATC 1 cut(s) 682
Sau96I GGNCC 4 cut(s) 178, 388, 450, 581
SchI GAGTC 2 cut(s) 33, 449
ScrFI CCNGG 2 cut(s) 203, 448
SduI GDGCHC 1 cut(s) 224
SetI ASST 1 cut(s) 293
SfaNI GCATC 1 cut(s) 328
SfoI GGCGCC 1 cut(s) 639
SfuI TTCGAA 1 cut(s) 557
SinI GGWCC 2 cut(s) 178, 450
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 4 cut(s) 259, 408, 608, 627
SseBI AGGCCT 1 cut(s) 147
SspDI GGCGCC 1 cut(s) 637
StuI AGGCCT 1 cut(s) 147
StyD4I CCNGG 2 cut(s) 201, 446
StyI CCWWGG 3 cut(s) 271, 384, 711
TaaI ACNGT 1 cut(s) 69
TaqI TCGA 5 cut(s) 168, 279, 369, 557, 685
TaqII GACCGA 1 cut(s) 467
TasI AATT 4 cut(s) 259, 408, 608, 627
TauI GCSGC 9 cut(s) 48, 57, 401, 404, 416, 419, 437, 468, 638
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 3 cut(s) 534, 651, 689
Tru9I TTAA 3 cut(s) 534, 651, 689
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 1 cut(s) 547
Tsp45I GTSAC 1 cut(s) 197
TspGWI ACGGA 1 cut(s) 217
Tth111I GACNNNGTC 1 cut(s) 203
VpaK11BI GGWCC 2 cut(s) 178, 450
XapI RAATTY 1 cut(s) 627
XceI RCATGY 1 cut(s) 363
XmiI GTMKAC 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.