Prupe.1G105300_v2.0.a1

dna binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009933
Physical Location & Seq
Forward (+)
8472786 .. 8474395
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G105300.2

Sequence Viewer

Length: 708 bp
ATGGCAATGAAGCGAGACCAACCCGACCTCGAGAAGCGACTCTGCGGCGTCTGCGGCTCCACAGAGCGTTGGTTCCTGCACTACGTCCGCCACAGAGGACATTTTCGTAAGCTCTGCACCAACTGCGTCCTCAAGAACAACCCAGGCCTCTTCTGCCCTATCTGCCTCGACTTCTTCGAGCACCCCCTTCCGGCACGTGACCAGGTCATGTGCGTCCGGTGCCCTTCCATCTCTCACTCTGCCTGCGTCGCAGCCAATTCCTCCTTCCGCAGCTTCCAGTGCCCTCCCTGCTCCCAGCCCACCTTCTCCTTCTTCAACCTCGCCCGCCAAAACGACGGCCAAGATGCCAAAACGACAGTCGTCATCGACAAGGACGCCGCCAAGGCCCTCGTCGCCGCCGCCAGGATCGCAGCGGCTTTGATGACCAAAGCTGCCGTCGCCGCCAGGGTTGACGCCGAGCGCCGGGTCAAGGAAGCCGTCGTGGCGAAAAAGAAGGCCAGAGAGGCCTTGGAACGACTCACGTCTCTTGTCAGTAAGGAAAAGGAGAAGGATTCGAAGAGTGGAGGCGTTGTTTCGGCGCCTGAGAGCCTCAACGCGAAGCCCAAATTGGAGGGTCCTGGGCCAATTCAAGTGGCGCCCAAGATTTCTGAGCCTGAGGGAAGTAATGGGTTGAAAACCAGTCATGCGGATCCTATGGAAGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

236

Amino Acids

25.65

Weight (kDa)

9.06

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 219, 577, 634
AccII CGCG 1 cut(s) 596
AclWI GGATC 3 cut(s) 413, 683, 696
AcoI YGGCCR 1 cut(s) 337
AcvI CACGTG 1 cut(s) 197
AcyI GRCGYC 5 cut(s) 48, 375, 453, 578, 635
AfiI CCNNNNNNNGG 4 cut(s) 190, 402, 462, 469
AgsI TTSAA 3 cut(s) 316, 629, 673
AjiI CACGTC 1 cut(s) 522
AjnI CCWGG 5 cut(s) 142, 201, 401, 443, 616
AjuI GAANNNNNNNTTGG 4 cut(s) 248, 280, 590, 622
AluBI AGCT 3 cut(s) 112, 273, 431
AluI AGCT 3 cut(s) 112, 273, 431
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 2 cut(s) 9, 528
AlwI GGATC 3 cut(s) 413, 683, 696
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 6 cut(s) 145, 337, 384, 495, 504, 620
ApeKI GCWGC 4 cut(s) 251, 270, 410, 431
ArsI GACNNNNNNTTYG 4 cut(s) 342, 374, 420, 452
AspLEI GCGC 3 cut(s) 462, 580, 637
AspS9I GGNCC 3 cut(s) 385, 614, 620
AsuC2I CCSGG 1 cut(s) 464
AsuII TTCGAA 1 cut(s) 554
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 614
AxyI CCTNAGG 1 cut(s) 654
BaeGI GKGCMC 2 cut(s) 224, 284
BamHI GGATCC 1 cut(s) 688
BanI GGYRCC 3 cut(s) 219, 577, 634
BbrPI CACGTG 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 4 cut(s) 263, 282, 418, 422
BccI CCATC 1 cut(s) 236
BceAI ACGGC 3 cut(s) 352, 419, 461
BciT130I CCWGG 5 cut(s) 144, 203, 403, 445, 618
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 2 cut(s) 9, 528
BfoI RGCGCY 3 cut(s) 463, 581, 638
BglI GCCNNNNNGGC 3 cut(s) 383, 482, 503
Bme1390I CCNGG 6 cut(s) 144, 203, 403, 445, 464, 618
Bme18I GGWCC 1 cut(s) 614
BmeT110I CYCGRG 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 522
BmgT120I GGNCC 3 cut(s) 385, 614, 620
BmiI GGNNCC 7 cut(s) 58, 74, 221, 579, 615, 636, 690
BmrFI CCNGG 6 cut(s) 144, 203, 403, 445, 464, 618
BmsI GCATC 1 cut(s) 334
BoxI GACNNNNGTC 2 cut(s) 359, 520
Bpu14I TTCGAA 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 1 cut(s) 464
BsaAI YACGTR 1 cut(s) 197
BsaHI GRCGYC 5 cut(s) 48, 375, 453, 578, 635
BsaI GGTCTC 1 cut(s) 9
BsaJI CCNNGG 5 cut(s) 142, 381, 444, 507, 617
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 402, 462, 469
Bse1I ACTGG 2 cut(s) 277, 678
Bse21I CCTNAGG 1 cut(s) 654
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 5 cut(s) 144, 203, 403, 445, 618
BseDI CCNNGG 5 cut(s) 142, 381, 444, 507, 617
BseLI CCNNNNNNNGG 4 cut(s) 190, 402, 462, 469
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 573, 639, 645
BseNI ACTGG 2 cut(s) 277, 678
BseSI GKGCMC 2 cut(s) 224, 284
BseXI GCAGC 4 cut(s) 263, 282, 418, 422
BseYI CCCAGC 1 cut(s) 294
BsgI GTGCAG 2 cut(s) 62, 100
Bsh1236I CGCG 1 cut(s) 596
BshFI GGCC 6 cut(s) 147, 339, 386, 497, 506, 622
BshNI GGYRCC 3 cut(s) 219, 577, 634
BsiHKAI GWGCWC 1 cut(s) 183
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 3 cut(s) 191, 217, 463
BslI CCNNNNNNNGG 4 cut(s) 190, 402, 462, 469
BsmAI GTCTC 2 cut(s) 9, 528
BsmBI CGTCTC 1 cut(s) 528
BsnI GGCC 6 cut(s) 147, 339, 386, 497, 506, 622
Bso31I GGTCTC 1 cut(s) 9
BsoBI CYCGRG 1 cut(s) 29
Bsp119I TTCGAA 1 cut(s) 554
Bsp1286I GDGCHC 3 cut(s) 183, 224, 284
Bsp143I GATC 2 cut(s) 405, 688
BspANI GGCC 6 cut(s) 147, 339, 386, 497, 506, 622
BspCNI CTCAG 3 cut(s) 574, 640, 646
BspFNI CGCG 1 cut(s) 596
BspLI GGNNCC 7 cut(s) 58, 74, 221, 579, 615, 636, 690
BspPI GGATC 3 cut(s) 413, 683, 696
BspT104I TTCGAA 1 cut(s) 554
BspT107I GGYRCC 3 cut(s) 219, 577, 634
BspTNI GGTCTC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 2 cut(s) 277, 678
BssECI CCNNGG 5 cut(s) 142, 381, 444, 507, 617
BssMI GATC 2 cut(s) 405, 688
BssNI GRCGYC 5 cut(s) 48, 375, 453, 578, 635
BssT1I CCWWGG 2 cut(s) 381, 507
Bst2UI CCWGG 5 cut(s) 144, 203, 403, 445, 618
Bst4CI ACNGT 1 cut(s) 358
Bst6I CTCTTC 2 cut(s) 155, 551
BstACI GRCGYC 5 cut(s) 48, 375, 453, 578, 635
BstAPI GCANNNNNTGC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 244, 325
BstDEI CTNAG 3 cut(s) 582, 648, 654
BstFNI CGCG 1 cut(s) 596
BstH2I RGCGCY 3 cut(s) 463, 581, 638
BstHHI GCGC 3 cut(s) 462, 580, 637
BstKTI GATC 2 cut(s) 408, 691
BstMAI GTCTC 2 cut(s) 9, 528
BstMBI GATC 2 cut(s) 405, 688
BstNI CCWGG 5 cut(s) 144, 203, 403, 445, 618
BstPAI GACNNNNGTC 2 cut(s) 359, 520
BstSCI CCNGG 6 cut(s) 142, 201, 401, 443, 462, 616
BstSLI GKGCMC 2 cut(s) 224, 284
BstUI CGCG 1 cut(s) 596
BstV1I GCAGC 4 cut(s) 263, 282, 418, 422
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
Bsu36I CCTNAGG 1 cut(s) 654
BsuRI GGCC 6 cut(s) 147, 339, 386, 497, 506, 622
BtrI CACGTC 1 cut(s) 522
BtsIMutI CAGTG 1 cut(s) 284
Cac8I GCNNGC 2 cut(s) 244, 325
CfoI GCGC 3 cut(s) 462, 580, 637
Cfr13I GGNCC 3 cut(s) 385, 614, 620
CseI GACGC 6 cut(s) 37, 115, 202, 235, 383, 461
CsiI ACCWGGT 1 cut(s) 201
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviAII CATG 2 cut(s) 208, 683
DdeI CTNAG 3 cut(s) 582, 648, 654
DinI GGCGCC 2 cut(s) 579, 636
DpnI GATC 2 cut(s) 407, 690
DpnII GATC 2 cut(s) 405, 688
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 2 cut(s) 155, 551
EarI CTCTTC 2 cut(s) 155, 551
EciI GGCGGA 1 cut(s) 77
Eco130I CCWWGG 2 cut(s) 381, 507
Eco147I AGGCCT 2 cut(s) 147, 506
Eco31I GGTCTC 1 cut(s) 9
Eco47I GGWCC 1 cut(s) 614
Eco72I CACGTG 1 cut(s) 197
Eco81I CCTNAGG 1 cut(s) 654
Eco88I CYCGRG 1 cut(s) 29
EcoO109I RGGNCCY 2 cut(s) 385, 614
EcoRII CCWGG 5 cut(s) 142, 201, 401, 443, 616
EcoT14I CCWWGG 2 cut(s) 381, 507
EgeI GGCGCC 2 cut(s) 579, 636
EheI GGCGCC 2 cut(s) 579, 636
ErhI CCWWGG 2 cut(s) 381, 507
Esp3I CGTCTC 1 cut(s) 528
FaeI CATG 2 cut(s) 211, 686
FaiI YATR 3 cut(s) 209, 684, 695
FatI CATG 2 cut(s) 207, 682
FauI CCCGC 1 cut(s) 332
GlaI GCGC 3 cut(s) 461, 579, 636
GsaI CCCAGC 1 cut(s) 298
HaeII RGCGCY 3 cut(s) 463, 581, 638
HaeIII GGCC 6 cut(s) 147, 339, 386, 497, 506, 622
HapII CCGG 3 cut(s) 191, 217, 463
HgaI GACGC 6 cut(s) 37, 115, 202, 235, 383, 461
HhaI GCGC 3 cut(s) 462, 580, 637
Hin1I GRCGYC 5 cut(s) 48, 375, 453, 578, 635
Hin1II CATG 2 cut(s) 211, 686
Hin6I GCGC 3 cut(s) 460, 578, 635
HinP1I GCGC 3 cut(s) 460, 578, 635
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 3 cut(s) 39, 516, 551
HpaII CCGG 3 cut(s) 191, 217, 463
Hpy166II GTNNAC 1 cut(s) 451
Hpy188I TCNGA 1 cut(s) 649
Hpy188III TCNNGA 2 cut(s) 31, 133
Hpy8I GTNNAC 1 cut(s) 451
Hpy99I CGWCG 5 cut(s) 251, 338, 395, 440, 482
HpyAV CCTTC 7 cut(s) 197, 234, 274, 313, 319, 487, 541
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4IV ACGT 3 cut(s) 84, 196, 521
HpyCH4V TGCA 2 cut(s) 79, 117
HpyF3I CTNAG 3 cut(s) 582, 648, 654
HpySE526I ACGT 3 cut(s) 84, 196, 521
Hsp92I GRCGYC 5 cut(s) 48, 375, 453, 578, 635
Hsp92II CATG 2 cut(s) 211, 686
HspAI GCGC 3 cut(s) 460, 578, 635
KasI GGCGCC 2 cut(s) 577, 634
Kzo9I GATC 2 cut(s) 405, 688
LmnI GCTCC 2 cut(s) 62, 296
Lsp1109I GCAGC 4 cut(s) 263, 282, 418, 422
LweI GCATC 1 cut(s) 334
MabI ACCWGGT 1 cut(s) 201
MaeII ACGT 3 cut(s) 84, 196, 521
MaeIII GTNAC 1 cut(s) 197
MalI GATC 2 cut(s) 407, 690
MboI GATC 2 cut(s) 405, 688
MboII GAAGA 4 cut(s) 142, 166, 304, 568
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 3 cut(s) 183, 224, 284
MluCI AATT 3 cut(s) 256, 605, 624
Mly113I GGCGCC 2 cut(s) 578, 635
MlyI GAGTC 2 cut(s) 33, 510
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 3 cut(s) 191, 217, 463
MspR9I CCNGG 6 cut(s) 144, 203, 403, 445, 464, 618
MvaI CCWGG 5 cut(s) 144, 203, 403, 445, 618
MvnI CGCG 1 cut(s) 596
NarI GGCGCC 2 cut(s) 578, 635
NciI CCSGG 1 cut(s) 464
NdeII GATC 2 cut(s) 405, 688
NlaIII CATG 2 cut(s) 211, 686
NlaIV GGNNCC 7 cut(s) 58, 74, 221, 579, 615, 636, 690
NmeAIII GCCGAG 1 cut(s) 481
NmuCI GTSAC 1 cut(s) 197
NspV TTCGAA 1 cut(s) 554
PaeR7I CTCGAG 1 cut(s) 29
PceI AGGCCT 2 cut(s) 147, 506
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 1 cut(s) 551
PflFI GACNNNGTC 1 cut(s) 203
PleI GAGTC 2 cut(s) 33, 510
PluTI GGCGCC 2 cut(s) 581, 638
PmaCI CACGTG 1 cut(s) 197
PmlI CACGTG 1 cut(s) 197
PpsI GAGTC 2 cut(s) 33, 510
Ppu21I YACGTR 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 614
PshAI GACNNNNGTC 2 cut(s) 359, 520
Psp5II RGGWCCY 1 cut(s) 614
Psp6I CCWGG 5 cut(s) 142, 201, 401, 443, 616
PspCI CACGTG 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 5 cut(s) 142, 201, 401, 443, 616
PspN4I GGNNCC 7 cut(s) 58, 74, 221, 579, 615, 636, 690
PspPI GGNCC 3 cut(s) 385, 614, 620
PspPPI RGGWCCY 1 cut(s) 614
PsuI RGATCY 1 cut(s) 688
PsyI GACNNNGTC 1 cut(s) 203
Sau3AI GATC 2 cut(s) 405, 688
Sau96I GGNCC 3 cut(s) 385, 614, 620
SchI GAGTC 2 cut(s) 33, 510
ScrFI CCNGG 6 cut(s) 144, 203, 403, 445, 464, 618
SduI GDGCHC 3 cut(s) 183, 224, 284
SexAI ACCWGGT 1 cut(s) 201
SfaNI GCATC 1 cut(s) 334
SfiI GGCCNNNNNGGCC 1 cut(s) 503
SfoI GGCGCC 2 cut(s) 579, 636
Sfr274I CTCGAG 1 cut(s) 29
SfuI TTCGAA 1 cut(s) 554
SinI GGWCC 1 cut(s) 614
SlaI CTCGAG 1 cut(s) 29
SmlI CTYRAG 2 cut(s) 29, 131
SmoI CTYRAG 2 cut(s) 29, 131
Sse9I AATT 3 cut(s) 256, 605, 624
SseBI AGGCCT 2 cut(s) 147, 506
SspDI GGCGCC 2 cut(s) 577, 634
StuI AGGCCT 2 cut(s) 147, 506
StyD4I CCNGG 6 cut(s) 142, 201, 401, 443, 462, 616
StyI CCWWGG 2 cut(s) 381, 507
TaaI ACNGT 1 cut(s) 358
TaiI ACGT 3 cut(s) 87, 199, 524
TaqI TCGA 5 cut(s) 30, 168, 177, 366, 554
TasI AATT 3 cut(s) 256, 605, 624
TauI GCSGC 7 cut(s) 48, 57, 380, 398, 401, 416, 443
TfiI GAWTC 1 cut(s) 551
TscAI CASTG 1 cut(s) 284
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 4 cut(s) 251, 270, 410, 431
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 1 cut(s) 23
TspRI CASTG 1 cut(s) 284
Tth111I GACNNNGTC 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 505
XhoI CTCGAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.