Rorug04G0218300

dna binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009933
Physical Location & Seq
Forward (+)
37713884 .. 37716294
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0218300.1

Sequence Viewer

Length: 429 bp
ATGGCTGCAGCGCCAGTAGCACAAGGAGTGAGTCTGAACCACATTTCCAGAGAATCCTCGGACATTAGGCGCCTCGCCGAGTTCTACAAAGAGACTTTTGGGTTCGAGGAGATAGAGAGCCCAAACTTTGGGGAATTCAAGGTGATATGGCTGAATTTACCTTCAGCTTTTTCTATGCACCTTATCGAGAGGAACCCAACTTACCAGCTTCCTGAAGGACCCTGGAGCGCCACGTCACCTGTTGCCGACCCAAGCCACCTTCCCAGGGGTCACCACATCTGCTTCTCTGTCTCCAATTTCGAATCTTTTGTGCAAACACTCAAGGAAAAGGGAATTCAGACTTTTCAGAGGTCACTACCTAATGGCAAGGTTAAGCAAGTATTTTTCTTTGATCCCGATGGCAACGGTTTGGAGGTTGCAGGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

15.91

Weight (kDa)

5.69

Isoelectric Point (pI)

59.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 12 - 139 1.3e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 14 - 123 9.8e-08 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 410
AccB1I GGYRCC 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 128
AclWI GGATC 1 cut(s) 386
AcsI RAATTY 3 cut(s) 134, 154, 333
AcuI CTGAAG 2 cut(s) 147, 234
AcyI GRCGYC 1 cut(s) 70
AfiI CCNNNNNNNGG 2 cut(s) 128, 265
AgsI TTSAA 1 cut(s) 139
AjiI CACGTC 1 cut(s) 234
AjnI CCWGG 2 cut(s) 221, 263
AloI GAACNNNNNNTCC 2 cut(s) 29, 61
AluBI AGCT 2 cut(s) 167, 208
AluI AGCT 2 cut(s) 167, 208
Alw26I GTCTC 2 cut(s) 86, 295
AlwI GGATC 1 cut(s) 386
ApeKI GCWGC 2 cut(s) 5, 8
ApoI RAATTY 3 cut(s) 134, 154, 333
AspLEI GCGC 3 cut(s) 13, 72, 230
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 3 cut(s) 154, 228, 263
AsuII TTCGAA 1 cut(s) 300
AvaII GGWCC 1 cut(s) 218
BanI GGYRCC 1 cut(s) 69
BanII GRGCYC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 20
BccI CCATC 1 cut(s) 392
BciT130I CCWGG 2 cut(s) 223, 265
BcoDI GTCTC 2 cut(s) 86, 295
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 3 cut(s) 14, 73, 231
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 2 cut(s) 6, 9
BlsI GCNGC 2 cut(s) 7, 10
Bme1390I CCNGG 2 cut(s) 223, 265
Bme18I GGWCC 1 cut(s) 218
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 3 cut(s) 71, 194, 220
BmrFI CCNGG 2 cut(s) 223, 265
BpmI CTGGAG 1 cut(s) 244
Bpu14I TTCGAA 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 305
BsaHI GRCGYC 1 cut(s) 70
BsaJI CCNNGG 4 cut(s) 57, 221, 263, 264
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 265
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 2 cut(s) 223, 265
BseDI CCNNGG 4 cut(s) 57, 221, 263, 264
BseLI CCNNNNNNNGG 2 cut(s) 128, 265
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 1 cut(s) 122
BseXI GCAGC 1 cut(s) 20
BshNI GGYRCC 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 128, 265
BsmAI GTCTC 2 cut(s) 86, 295
Bsp119I TTCGAA 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 122
Bsp143I GATC 1 cut(s) 391
BspLI GGNNCC 3 cut(s) 71, 194, 220
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 1 cut(s) 386
BspT104I TTCGAA 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 69
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 4 cut(s) 57, 221, 263, 264
BssMI GATC 1 cut(s) 391
BssNI GRCGYC 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 223, 265
Bst4CI ACNGT 1 cut(s) 407
BstACI GRCGYC 1 cut(s) 70
BstBI TTCGAA 1 cut(s) 300
BstEII GGTNACC 1 cut(s) 269
BstH2I RGCGCY 3 cut(s) 14, 73, 231
BstHHI GCGC 3 cut(s) 13, 72, 230
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 2 cut(s) 86, 295
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 2 cut(s) 223, 265
BstPI GGTNACC 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 221, 263
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 1 cut(s) 20
BtrI CACGTC 1 cut(s) 234
BveI ACCTGC 1 cut(s) 410
CfoI GCGC 3 cut(s) 13, 72, 230
Cfr13I GGNCC 1 cut(s) 218
CviJI RGCY 6 cut(s) 5, 120, 151, 167, 208, 255
CviKI_1 RGCY 6 cut(s) 5, 120, 151, 167, 208, 255
DinI GGCGCC 1 cut(s) 71
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 218
Eco57I CTGAAG 2 cut(s) 147, 234
Eco91I GGTNACC 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 218
EcoO65I GGTNACC 1 cut(s) 269
EcoRI GAATTC 2 cut(s) 134, 333
EcoRII CCWGG 2 cut(s) 221, 263
EcoT38I GRGCYC 1 cut(s) 122
EgeI GGCGCC 1 cut(s) 71
EheI GGCGCC 1 cut(s) 71
FaiI YATR 2 cut(s) 148, 176
Fnu4HI GCNGC 2 cut(s) 6, 9
FriOI GRGCYC 1 cut(s) 122
Fsp4HI GCNGC 2 cut(s) 6, 9
GlaI GCGC 3 cut(s) 12, 71, 229
GluI GCNGC 2 cut(s) 6, 9
GsuI CTGGAG 1 cut(s) 244
HaeII RGCGCY 3 cut(s) 14, 73, 231
HhaI GCGC 3 cut(s) 13, 72, 230
Hin1I GRCGYC 1 cut(s) 70
Hin6I GCGC 3 cut(s) 11, 70, 228
HinP1I GCGC 3 cut(s) 11, 70, 228
HinfI GANTC 3 cut(s) 31, 53, 302
HphI GGTGA 3 cut(s) 154, 228, 263
Hpy188I TCNGA 4 cut(s) 36, 61, 339, 348
Hpy188III TCNNGA 4 cut(s) 48, 187, 212, 395
HpyAV CCTTC 3 cut(s) 171, 209, 269
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 4 cut(s) 8, 178, 313, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpySE526I ACGT 1 cut(s) 233
Hsp92I GRCGYC 1 cut(s) 70
HspAI GCGC 3 cut(s) 11, 70, 228
KasI GGCGCC 1 cut(s) 69
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 225
Lsp1109I GCAGC 1 cut(s) 20
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 3 cut(s) 234, 269, 351
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 4 cut(s) 134, 154, 295, 333
Mly113I GGCGCC 1 cut(s) 70
MlyI GAGTC 1 cut(s) 40
MnlI CCTC 6 cut(s) 67, 83, 100, 183, 342, 406
MseI TTAA 1 cut(s) 372
MspR9I CCNGG 2 cut(s) 223, 265
MvaI CCWGG 2 cut(s) 223, 265
MwoI GCNNNNNNNGC 1 cut(s) 17
NarI GGCGCC 1 cut(s) 70
NdeII GATC 1 cut(s) 391
NlaIV GGNNCC 3 cut(s) 71, 194, 220
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 3 cut(s) 234, 269, 351
NspV TTCGAA 1 cut(s) 300
PasI CCCWGGG 1 cut(s) 264
PfeI GAWTC 2 cut(s) 53, 302
PflMI CCANNNNNTGG 1 cut(s) 128
PkrI GCNGC 2 cut(s) 7, 10
PleI GAGTC 1 cut(s) 39
PluTI GGCGCC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 39
PpuMI RGGWCCY 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 2 cut(s) 221, 263
PspEI GGTNACC 1 cut(s) 269
PspGI CCWGG 2 cut(s) 221, 263
PspN4I GGNNCC 3 cut(s) 71, 194, 220
PspPI GGNCC 1 cut(s) 218
PspPPI RGGWCCY 1 cut(s) 218
PsrI GAACNNNNNNTAC 2 cut(s) 185, 217
PstI CTGCAG 1 cut(s) 10
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 2 cut(s) 223, 265
SduI GDGCHC 1 cut(s) 122
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 71
SfuI TTCGAA 1 cut(s) 300
SinI GGWCC 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 4 cut(s) 134, 154, 295, 333
SspDI GGCGCC 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 221, 263
TaaI ACNGT 1 cut(s) 407
TaiI ACGT 1 cut(s) 236
TaqI TCGA 3 cut(s) 105, 186, 300
TasI AATT 4 cut(s) 134, 154, 295, 333
TfiI GAWTC 2 cut(s) 53, 302
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 3 cut(s) 234, 269, 351
TseI GCWGC 2 cut(s) 5, 8
Tsp45I GTSAC 3 cut(s) 234, 269, 351
Van91I CCANNNNNTGG 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 3 cut(s) 134, 154, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.