MD16G1174400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009250
Physical Location & Seq
Forward (+)
14733504 .. 14737256
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1174400.v1.1.491

Sequence Viewer

Length: 1206 bp
ATGCAAATGATCTCCAACACTCGCAGGCTCTCCAGAGCTTTCAAATCTCGCATTTTTATAAAATCCGCCGACCAATTTCTCTCCCCCGATGCCCGACTCGTCGAAGCTCCCCTCACTTCTCTCCAATGGCGGAAACTAAGCGCCGGCTCTTCCCAAACTGCCTTGTTATCCGGCATTACTTGCGGTCATCTTTTGCCGATGGAACAACGTGCAACATGTGCAGCGATGACATTGAGAGCACCCTTTTCTTCTGAAGCAAGTACCATCGAAGGTGGTTCCACAGAGGTTGTGAAGGACCTGTATGATAAAATGCTGCAGTCTGTTAATATCAAACGATCAATGCCCCCTAATGCTTGGTTATGGTCATTGATTGAAAATTGCAAAAAGGAAGAAGATATTAAGCTTCTATTTGACATCTTGCAGAACCTTCGAAGATTTAGACTGTCAAATCTTCGCATCCATTCTGATTTTAATTGCAATCTCTGCCGAGAGGTTACTAAGGCATGTGTTTGTGTGGGAGCTCTTGACTTTGGAAAGAAGGCCTTGTGGAAACATAATGTGTATGGATTGGATCCTAGCATTGGATCTGCGCACCATTTACTGTTGTATGCTAAAGAACGCAATGATGCTAAACTTATGGTGGAAGTAATGAAACTTTTGAAGAGGAATGACTTACCCTTGCAACCCGGTACTGCAGATATTGTTTTCAGCATTTGTTACAATACCGATAATTGGCAGCTGATGTCAAAGTACTCAAAAAGGTTTGTCAAAGCTGGAGTAAAGCTACGTCAAACTGCTTTTGATTCATGGATGGATTTTGCTGCTAAAATAGGAGATGTTGAATCATTGTGGAAAGCTGAGAAGTTAAGATCTGAATCAATGAAGCAGCATACTGTTGCAAGTGGACTTTCATGTGCTAAGGGTTTTCTCTTAGAACGTAAGCCCGAGGAAGCTGCTTCCGTTATTCAAGTTCTAAATCAGATTTTACCTGATACAAAGAAGTCCCGCATCGTGGTTGAGCTTGAAAAGTTGGTTAGGGAGTGGCCCTTAGAAGTTATTAGGCGCCAGAAGGAAGAGGACAGAAAGGCATTAGCTGCATCTTTAATCGCTGATATCCCTACAATGGTTAGTTCCCTATTAAACACGGGCTTACAAGTGAGCGCAAACATTGAGGACCTAACCACCAAAGAAGGTATTCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

45.29

Weight (kDa)

9.27

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 59
Acc16I TGCGCA 1 cut(s) 591
AccB1I GGYRCC 1 cut(s) 1062
AciI CCGC 4 cut(s) 66, 130, 183, 1006
AclWI GGATC 3 cut(s) 566, 579, 592
AcuI CTGAAG 1 cut(s) 273
AcyI GRCGYC 1 cut(s) 1063
AfaI GTAC 3 cut(s) 262, 691, 752
AfiI CCNNNNNNNGG 4 cut(s) 581, 732, 1012, 1123
AflIII ACRYGT 1 cut(s) 215
AgsI TTSAA 6 cut(s) 43, 374, 661, 842, 968, 1025
Alw21I GWGCWC 2 cut(s) 241, 523
AlwI GGATC 3 cut(s) 566, 579, 592
Ama87I CYCGRG 1 cut(s) 944
AoxI GGCC 2 cut(s) 540, 1043
ApeKI GCWGC 7 cut(s) 221, 313, 736, 821, 886, 953, 1094
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 4 cut(s) 143, 592, 1065, 1163
AspS9I GGNCC 3 cut(s) 295, 1044, 1174
AsuC2I CCSGG 1 cut(s) 687
AsuII TTCGAA 1 cut(s) 430
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 2 cut(s) 295, 1174
BamHI GGATCC 1 cut(s) 571
BanI GGYRCC 1 cut(s) 1062
BanII GRGCYC 1 cut(s) 523
Bbv12I GWGCWC 2 cut(s) 241, 523
BbvI GCAGC 7 cut(s) 233, 300, 748, 808, 898, 940, 1081
BccI CCATC 3 cut(s) 193, 272, 805
BcgI CGANNNNNNTGC 4 cut(s) 410, 444, 935, 969
BcnI CCSGG 1 cut(s) 687
BfaI CTAG 1 cut(s) 576
BfmI CTRYAG 2 cut(s) 314, 693
BfoI RGCGCY 2 cut(s) 144, 1066
BglII AGATCT 1 cut(s) 869
BisI GCNGC 7 cut(s) 222, 314, 737, 822, 887, 954, 1095
BlsI GCNGC 7 cut(s) 223, 315, 738, 823, 888, 955, 1096
BmcAI AGTACT 1 cut(s) 752
Bme1390I CCNGG 1 cut(s) 687
Bme18I GGWCC 2 cut(s) 295, 1174
BmeT110I CYCGRG 1 cut(s) 944
BmgT120I GGNCC 3 cut(s) 295, 1044, 1174
BmiI GGNNCC 3 cut(s) 277, 573, 1064
BmrFI CCNGG 1 cut(s) 687
BmsI GCATC 5 cut(s) 79, 465, 616, 1017, 1106
BpmI CTGGAG 2 cut(s) 16, 795
Bpu10I CCTNAGC 1 cut(s) 918
Bpu14I TTCGAA 1 cut(s) 430
BpuMI CCSGG 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 874
BsaHI GRCGYC 1 cut(s) 1063
BsaJI CCNNGG 1 cut(s) 945
Bsc4I CCNNNNNNNGG 4 cut(s) 581, 732, 1012, 1123
Bse118I RCCGGY 1 cut(s) 143
Bse3DI GCAATG 1 cut(s) 628
Bse8I GATNNNNATC 1 cut(s) 874
BseDI CCNNGG 1 cut(s) 945
BseGI GGATG 2 cut(s) 456, 816
BseJI GATNNNNATC 1 cut(s) 874
BseLI CCNNNNNNNGG 4 cut(s) 581, 732, 1012, 1123
BseMI GCAATG 1 cut(s) 628
BseMII CTCAG 1 cut(s) 849
BseXI GCAGC 7 cut(s) 233, 300, 748, 808, 898, 940, 1081
BsgI GTGCAG 1 cut(s) 240
BshFI GGCC 2 cut(s) 542, 1045
BshNI GGYRCC 1 cut(s) 1062
BsiHKAI GWGCWC 2 cut(s) 241, 523
BsiHKCI CYCGRG 1 cut(s) 944
BsiSI CCGG 3 cut(s) 144, 171, 687
BslFI GGGAC 1 cut(s) 988
BslI CCNNNNNNNGG 4 cut(s) 581, 732, 1012, 1123
BsmFI GGGAC 1 cut(s) 988
BsnI GGCC 2 cut(s) 542, 1045
BsoBI CYCGRG 1 cut(s) 944
Bsp119I TTCGAA 1 cut(s) 430
Bsp1286I GDGCHC 2 cut(s) 241, 523
Bsp143I GATC 5 cut(s) 9, 335, 571, 584, 869
BspACI CCGC 4 cut(s) 66, 130, 183, 1006
BspANI GGCC 2 cut(s) 542, 1045
BspCNI CTCAG 1 cut(s) 850
BspLI GGNNCC 3 cut(s) 277, 573, 1064
BspMAI CTGCAG 2 cut(s) 318, 697
BspPI GGATC 3 cut(s) 566, 579, 592
BspQI GCTCTTC 1 cut(s) 154
BspT104I TTCGAA 1 cut(s) 430
BspT107I GGYRCC 1 cut(s) 1062
BsrDI GCAATG 1 cut(s) 628
BsrFI RCCGGY 1 cut(s) 143
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 945
BssMI GATC 5 cut(s) 9, 335, 571, 584, 869
BssNI GRCGYC 1 cut(s) 1063
Bst4CI ACNGT 3 cut(s) 444, 603, 895
Bst6I CTCTTC 3 cut(s) 154, 656, 1068
BstACI GRCGYC 1 cut(s) 1063
BstAPI GCANNNNNTGC 4 cut(s) 180, 218, 483, 1094
BstBI TTCGAA 1 cut(s) 430
BstC8I GCNNGC 2 cut(s) 26, 145
BstDEI CTNAG 6 cut(s) 137, 498, 858, 918, 931, 1048
BstF5I GGATG 2 cut(s) 456, 816
BstH2I RGCGCY 2 cut(s) 144, 1066
BstHHI GCGC 4 cut(s) 143, 592, 1065, 1163
BstKTI GATC 5 cut(s) 12, 338, 574, 587, 872
BstMBI GATC 5 cut(s) 9, 335, 571, 584, 869
BstMWI GCNNNNNNNGC 4 cut(s) 180, 218, 483, 1094
BstNSI RCATGY 2 cut(s) 219, 507
BstSCI CCNGG 1 cut(s) 685
BstSFI CTRYAG 2 cut(s) 314, 693
BstV1I GCAGC 7 cut(s) 233, 300, 748, 808, 898, 940, 1081
BstX2I RGATCY 3 cut(s) 571, 584, 869
BstYI RGATCY 3 cut(s) 571, 584, 869
BsuRI GGCC 2 cut(s) 542, 1045
BtgZI GCGATG 1 cut(s) 239
BtsCI GGATG 2 cut(s) 456, 816
Cac8I GCNNGC 2 cut(s) 26, 145
CfoI GCGC 4 cut(s) 143, 592, 1065, 1163
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 3 cut(s) 295, 1044, 1174
Csp6I GTAC 3 cut(s) 261, 690, 751
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 4 cut(s) 216, 504, 807, 912
CviQI GTAC 3 cut(s) 261, 690, 751
DdeI CTNAG 6 cut(s) 137, 498, 858, 918, 931, 1048
DinI GGCGCC 1 cut(s) 1064
DpnI GATC 5 cut(s) 11, 337, 573, 586, 871
DpnII GATC 5 cut(s) 9, 335, 571, 584, 869
Eam1104I CTCTTC 3 cut(s) 154, 656, 1068
EarI CTCTTC 3 cut(s) 154, 656, 1068
EciI GGCGGA 2 cut(s) 55, 145
Ecl136II GAGCTC 1 cut(s) 521
Eco147I AGGCCT 1 cut(s) 542
Eco24I GRGCYC 1 cut(s) 523
Eco32I GATATC 1 cut(s) 1114
Eco47I GGWCC 2 cut(s) 295, 1174
Eco53kI GAGCTC 1 cut(s) 521
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 944
EcoICRI GAGCTC 1 cut(s) 521
EcoO109I RGGNCCY 2 cut(s) 295, 1174
EcoRV GATATC 1 cut(s) 1114
EcoT38I GRGCYC 1 cut(s) 523
EgeI GGCGCC 1 cut(s) 1064
EheI GGCGCC 1 cut(s) 1064
FaeI CATG 4 cut(s) 219, 507, 810, 915
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FaqI GGGAC 1 cut(s) 988
FatI CATG 4 cut(s) 215, 503, 806, 911
FauI CCCGC 1 cut(s) 1013
Fnu4HI GCNGC 7 cut(s) 222, 314, 737, 822, 887, 954, 1095
FokI GGATG 2 cut(s) 443, 823
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 7 cut(s) 222, 314, 737, 822, 887, 954, 1095
FspBI CTAG 1 cut(s) 576
FspI TGCGCA 1 cut(s) 591
GlaI GCGC 4 cut(s) 142, 591, 1064, 1162
GluI GCNGC 7 cut(s) 222, 314, 737, 822, 887, 954, 1095
GsuI CTGGAG 2 cut(s) 16, 795
HaeII RGCGCY 2 cut(s) 144, 1066
HaeIII GGCC 2 cut(s) 542, 1045
HapII CCGG 3 cut(s) 144, 171, 687
HhaI GCGC 4 cut(s) 143, 592, 1065, 1163
Hin1I GRCGYC 1 cut(s) 1063
Hin1II CATG 4 cut(s) 219, 507, 810, 915
Hin6I GCGC 4 cut(s) 141, 590, 1063, 1161
HinP1I GCGC 4 cut(s) 141, 590, 1063, 1161
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 4 cut(s) 96, 803, 842, 875
HpaII CCGG 3 cut(s) 144, 171, 687
Hpy166II GTNNAC 1 cut(s) 905
Hpy188I TCNGA 4 cut(s) 253, 466, 874, 981
Hpy188III TCNNGA 2 cut(s) 33, 524
Hpy8I GTNNAC 1 cut(s) 905
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 6 cut(s) 263, 286, 437, 532, 1063, 1184
HpyCH4III ACNGT 3 cut(s) 444, 603, 895
HpyCH4IV ACGT 3 cut(s) 208, 787, 937
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 218, 483, 1094
HpyF3I CTNAG 6 cut(s) 137, 498, 858, 918, 931, 1048
HpySE526I ACGT 3 cut(s) 208, 787, 937
Hsp92I GRCGYC 1 cut(s) 1063
Hsp92II CATG 4 cut(s) 219, 507, 810, 915
HspAI GCGC 4 cut(s) 141, 590, 1063, 1161
KasI GGCGCC 1 cut(s) 1062
KroI GCCGGC 1 cut(s) 143
KroNI GCCGGC 1 cut(s) 145
Kzo9I GATC 5 cut(s) 9, 335, 571, 584, 869
LguI GCTCTTC 1 cut(s) 154
LmnI GCTCC 2 cut(s) 112, 518
LpnPI CCDG 9 cut(s) 10, 46, 157, 184, 311, 700, 759, 1002, 1079
Lsp1109I GCAGC 7 cut(s) 233, 300, 748, 808, 898, 940, 1081
LweI GCATC 5 cut(s) 79, 465, 616, 1017, 1106
MaeI CTAG 1 cut(s) 576
MaeII ACGT 3 cut(s) 208, 787, 937
MaeIII GTNAC 2 cut(s) 493, 716
MalI GATC 5 cut(s) 11, 337, 573, 586, 871
MboI GATC 5 cut(s) 9, 335, 571, 584, 869
MboII GAAGA 8 cut(s) 141, 240, 401, 404, 443, 444, 673, 1085
MflI RGATCY 3 cut(s) 571, 584, 869
MhlI GDGCHC 2 cut(s) 241, 523
MluCI AATT 4 cut(s) 74, 376, 472, 730
Mly113I GGCGCC 1 cut(s) 1063
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 7 cut(s) 122, 277, 484, 657, 940, 1069, 1165
MroNI GCCGGC 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 6 cut(s) 324, 399, 471, 866, 1103, 1139
MspA1I CMGCKG 1 cut(s) 739
MspI CCGG 3 cut(s) 144, 171, 687
MspR9I CCNGG 1 cut(s) 687
MwoI GCNNNNNNNGC 4 cut(s) 180, 218, 483, 1094
NaeI GCCGGC 1 cut(s) 145
NarI GGCGCC 1 cut(s) 1063
NciI CCSGG 1 cut(s) 687
NdeII GATC 5 cut(s) 9, 335, 571, 584, 869
NgoMIV GCCGGC 1 cut(s) 143
NlaIII CATG 4 cut(s) 219, 507, 810, 915
NlaIV GGNNCC 3 cut(s) 277, 573, 1064
NmeAIII GCCGAG 1 cut(s) 512
NsbI TGCGCA 1 cut(s) 591
NspI RCATGY 2 cut(s) 219, 507
NspV TTCGAA 1 cut(s) 430
PceI AGGCCT 1 cut(s) 542
PciI ACATGT 1 cut(s) 215
PciSI GCTCTTC 1 cut(s) 154
PdiI GCCGGC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 3 cut(s) 803, 842, 875
PkrI GCNGC 7 cut(s) 223, 315, 738, 823, 888, 955, 1096
PleI GAGTC 1 cut(s) 90
PluTI GGCGCC 1 cut(s) 1066
PpsI GAGTC 1 cut(s) 90
PpuMI RGGWCCY 2 cut(s) 295, 1174
PscI ACATGT 1 cut(s) 215
PsiI TTATAA 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 523
Psp5II RGGWCCY 2 cut(s) 295, 1174
PspN4I GGNNCC 3 cut(s) 277, 573, 1064
PspPI GGNCC 3 cut(s) 295, 1044, 1174
PspPPI RGGWCCY 2 cut(s) 295, 1174
PstI CTGCAG 2 cut(s) 318, 697
PsuI RGATCY 3 cut(s) 571, 584, 869
PvuII CAGCTG 1 cut(s) 739
RsaI GTAC 3 cut(s) 262, 691, 752
RsaNI GTAC 3 cut(s) 261, 690, 751
SacI GAGCTC 1 cut(s) 523
SapI GCTCTTC 1 cut(s) 154
SaqAI TTAA 6 cut(s) 324, 399, 471, 866, 1103, 1139
SatI GCNGC 7 cut(s) 222, 314, 737, 822, 887, 954, 1095
Sau3AI GATC 5 cut(s) 9, 335, 571, 584, 869
Sau96I GGNCC 3 cut(s) 295, 1044, 1174
ScaI AGTACT 1 cut(s) 752
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 687
SduI GDGCHC 2 cut(s) 241, 523
SfaNI GCATC 5 cut(s) 79, 465, 616, 1017, 1106
SfcI CTRYAG 2 cut(s) 314, 693
SfoI GGCGCC 1 cut(s) 1064
SfuI TTCGAA 1 cut(s) 430
SinI GGWCC 2 cut(s) 295, 1174
Sse9I AATT 4 cut(s) 74, 376, 472, 730
SseBI AGGCCT 1 cut(s) 542
SsiI CCGC 4 cut(s) 66, 130, 183, 1006
SspDI GGCGCC 1 cut(s) 1062
SspMI CTAG 1 cut(s) 576
SstI GAGCTC 1 cut(s) 523
StuI AGGCCT 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 685
TaaI ACNGT 3 cut(s) 444, 603, 895
TaiI ACGT 3 cut(s) 211, 790, 940
TaqI TCGA 3 cut(s) 102, 267, 430
TasI AATT 4 cut(s) 74, 376, 472, 730
TatI WGTACW 1 cut(s) 750
TfiI GAWTC 3 cut(s) 803, 842, 875
Tru1I TTAA 6 cut(s) 324, 399, 471, 866, 1103, 1139
Tru9I TTAA 6 cut(s) 324, 399, 471, 866, 1103, 1139
TseI GCWGC 7 cut(s) 221, 313, 736, 821, 886, 953, 1094
TspDTI ATGAA 5 cut(s) 665, 795, 896, 900, 1187
TspGWI ACGGA 1 cut(s) 949
VpaK11BI GGWCC 2 cut(s) 295, 1174
XceI RCATGY 2 cut(s) 219, 507
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 1 cut(s) 576
ZrmI AGTACT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.