pycom13g14850

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009250
Physical Location & Seq
Forward (+)
10748716 .. 10751946
3231 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g14850.8

Sequence Viewer

Length: 1212 bp
ATGCAAGTGATCTCCAATACTCGCAGGCTCTCCAGAGCTTTCAAATCTCCCATTTGTATCAAATCCACCGACCAATTTCTCTCTCCCGATGCCCGAGTCGTCGAAGCTCCACTCTCTTCTCTCCAATGGCGGAACCTCGGCGCCGGCTCTTCCCAAACGGCCTTGTTCTCAGGCAGTACTCGCGGTCATCTTTTGCTGATGGAACAACATTCAACTTGTGCAGTGATGATGTTGAGAGCGCCGTTTTCTTCTGAAGCGAGTACCATCCAAGGTGGTTCCACAGAGGCTGTGAAGGAGCTGTCTGATAAGATGTTGCAGTCTGTTAATATTAAACGATCGATCCCCCCTAATGCTTGGTTATGGTCATTGATTGAAAATTGCAAAAAGGAAGGAGATATTAAGCTTCTATTTGACATCTTGCAGAACCTTCGAAGATTTAGACTGTCAAATCTTCGCATCCATTCCGATTTTAATTGCAATCTCTGCCGAGAGGTTACTAAGGCGTGTGTTCGTGTGGGAGCTCTTGACTATGGAAAGAAGGCCTTGTGGAAACATAACGTGTATGGATTGGCTCCTAGCATCGGATCGATGCACACCATTTACTGGCAGTTGTATGCTAAAGAACGCAATGATTCTAAACTTATGGTGGAAGTAATGAAACTTTTGAAGAGGAATGGCCTACCATTGCAACCTGGTACAGCAGATATTGTTTTCAGCATTTGTTACAATACTGATAATTGGCAGTTGATGTCTAAGTACTCAAAAAGGTTTGTCAAAGCTGGAGTAAAGCTACGTCAAACTGCTTTCGATCTATGGATGGAATTTGCTGCTAAAATAGGAGATGTTGAATCATTGTGGAAAGCAGAGAAACTAAGATCTGAATTGATGAAGCAGCATACTGTTGCAAGTGGACTTTCATGTGCTAAGGGTTTTCTGTTAGAATGTAAGCCCGAGGAAGCTGCTTCCATCATTCAAGTTCTAAATCAGATTTTACCTGATACAAAGAAGTCCAGTATTGTGGTTGGGCTTGAAAAGTTAGTTAGTGAGTGGCCCTTAGAGGTTATTAGGCGCCAGAAGGAAGAGGACAGAAAGGCATTAGCTACATCTTTGATCTCTGATATCCCTACAATGGTTAGTTCCCTATCAAACACGGGCTTACAAGTGAGCGTAAACATTGATGACCTAACCAGCAAAGAAGGTATTCTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

404

Amino Acids

45.25

Weight (kDa)

9.33

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 140, 1068
AccB7I CCANNNNNTGG 1 cut(s) 603
AccII CGCG 1 cut(s) 183
AciI CCGC 2 cut(s) 130, 183
AclWI GGATC 2 cut(s) 334, 592
AcsI RAATTY 1 cut(s) 821
AcuI CTGAAG 1 cut(s) 273
AcyI GRCGYC 2 cut(s) 141, 1069
AfaI GTAC 4 cut(s) 178, 262, 697, 758
AfiI CCNNNNNNNGG 3 cut(s) 581, 603, 1129
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 7 cut(s) 43, 213, 374, 667, 848, 974, 1031
AjnI CCWGG 1 cut(s) 691
AluBI AGCT 9 cut(s) 38, 107, 298, 403, 521, 779, 790, 959, 1100
AluI AGCT 9 cut(s) 38, 107, 298, 403, 521, 779, 790, 959, 1100
Alw21I GWGCWC 1 cut(s) 523
AlwI GGATC 2 cut(s) 334, 592
AlwNI CAGNNNCTG 1 cut(s) 287
Ama87I CYCGRG 2 cut(s) 93, 950
AoxI GGCC 4 cut(s) 159, 540, 676, 1049
ApeKI GCWGC 3 cut(s) 827, 892, 959
ApoI RAATTY 1 cut(s) 821
Asp700I GAANNNNTTC 1 cut(s) 1200
AspLEI GCGC 3 cut(s) 143, 241, 1071
AspS9I GGNCC 1 cut(s) 1050
AsuII TTCGAA 1 cut(s) 430
AvaI CYCGRG 2 cut(s) 93, 950
BanI GGYRCC 2 cut(s) 140, 1068
BanII GRGCYC 1 cut(s) 523
Bbv12I GWGCWC 1 cut(s) 523
BbvI GCAGC 3 cut(s) 814, 904, 946
BccI CCATC 4 cut(s) 193, 272, 811, 974
BceAI ACGGC 2 cut(s) 174, 226
BcgI CGANNNNNNTGC 4 cut(s) 410, 444, 941, 975
BciT130I CCWGG 1 cut(s) 693
BfaI CTAG 1 cut(s) 576
BfoI RGCGCY 3 cut(s) 144, 242, 1072
BglII AGATCT 1 cut(s) 875
BisI GCNGC 3 cut(s) 828, 893, 960
BlsI GCNGC 3 cut(s) 829, 894, 961
BmcAI AGTACT 2 cut(s) 178, 758
Bme1390I CCNGG 1 cut(s) 693
BmeT110I CYCGRG 2 cut(s) 93, 950
BmgT120I GGNCC 1 cut(s) 1050
BmiI GGNNCC 5 cut(s) 134, 142, 277, 573, 1070
BmrFI CCNGG 1 cut(s) 693
BmsI GCATC 4 cut(s) 79, 465, 579, 588
BpmI CTGGAG 2 cut(s) 16, 801
Bpu10I CCTNAGC 1 cut(s) 924
Bpu14I TTCGAA 1 cut(s) 430
Bsa29I ATCGAT 2 cut(s) 338, 587
BsaHI GRCGYC 2 cut(s) 141, 1069
BsaJI CCNNGG 3 cut(s) 136, 268, 951
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 603, 1129
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 2 cut(s) 608, 1011
Bse3DI GCAATG 2 cut(s) 634, 683
BseBI CCWGG 1 cut(s) 693
BseCI ATCGAT 2 cut(s) 338, 587
BseDI CCNNGG 3 cut(s) 136, 268, 951
BseGI GGATG 3 cut(s) 264, 456, 822
BseLI CCNNNNNNNGG 3 cut(s) 581, 603, 1129
BseMI GCAATG 2 cut(s) 634, 683
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 2 cut(s) 608, 1011
BseXI GCAGC 3 cut(s) 814, 904, 946
BsgI GTGCAG 1 cut(s) 240
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 338
BshFI GGCC 4 cut(s) 161, 542, 678, 1051
BshNI GGYRCC 2 cut(s) 140, 1068
BshVI ATCGAT 2 cut(s) 338, 587
BsiEI CGRYCG 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 523
BsiHKCI CYCGRG 2 cut(s) 93, 950
BsiSI CCGG 1 cut(s) 144
BslI CCNNNNNNNGG 3 cut(s) 581, 603, 1129
BsnI GGCC 4 cut(s) 161, 542, 678, 1051
BsoBI CYCGRG 2 cut(s) 93, 950
Bsp119I TTCGAA 1 cut(s) 430
Bsp1286I GDGCHC 1 cut(s) 523
Bsp143I GATC 7 cut(s) 9, 335, 339, 584, 808, 875, 1110
BspACI CCGC 2 cut(s) 130, 183
BspANI GGCC 4 cut(s) 161, 542, 678, 1051
BspCNI CTCAG 1 cut(s) 182
BspDI ATCGAT 2 cut(s) 338, 587
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 5 cut(s) 134, 142, 277, 573, 1070
BspPI GGATC 2 cut(s) 334, 592
BspQI GCTCTTC 1 cut(s) 154
BspT104I TTCGAA 1 cut(s) 430
BspT107I GGYRCC 2 cut(s) 140, 1068
BsrDI GCAATG 2 cut(s) 634, 683
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 2 cut(s) 608, 1011
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 3 cut(s) 136, 268, 951
BssMI GATC 7 cut(s) 9, 335, 339, 584, 808, 875, 1110
BssNI GRCGYC 2 cut(s) 141, 1069
BssT1I CCWWGG 1 cut(s) 268
Bst2UI CCWGG 1 cut(s) 693
Bst4CI ACNGT 2 cut(s) 444, 901
Bst6I CTCTTC 4 cut(s) 121, 154, 662, 1074
BstACI GRCGYC 2 cut(s) 141, 1069
BstAPI GCANNNNNTGC 1 cut(s) 483
BstBI TTCGAA 1 cut(s) 430
BstC8I GCNNGC 2 cut(s) 26, 145
BstDEI CTNAG 6 cut(s) 169, 498, 753, 872, 924, 1054
BstF5I GGATG 3 cut(s) 264, 456, 822
BstFNI CGCG 1 cut(s) 183
BstH2I RGCGCY 3 cut(s) 144, 242, 1072
BstHHI GCGC 3 cut(s) 143, 241, 1071
BstKTI GATC 7 cut(s) 12, 338, 342, 587, 811, 878, 1113
BstMBI GATC 7 cut(s) 9, 335, 339, 584, 808, 875, 1110
BstMCI CGRYCG 1 cut(s) 338
BstMWI GCNNNNNNNGC 2 cut(s) 180, 483
BstNI CCWGG 1 cut(s) 693
BstSCI CCNGG 1 cut(s) 691
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 814, 904, 946
BstX2I RGATCY 1 cut(s) 875
BstXI CCANNNNNNTGG 1 cut(s) 1018
BstYI RGATCY 1 cut(s) 875
Bsu15I ATCGAT 2 cut(s) 338, 587
BsuRI GGCC 4 cut(s) 161, 542, 678, 1051
BsuTUI ATCGAT 2 cut(s) 338, 587
BtsCI GGATG 3 cut(s) 264, 456, 822
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 2 cut(s) 26, 145
CaiI CAGNNNCTG 1 cut(s) 287
CfoI GCGC 3 cut(s) 143, 241, 1071
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 1050
ClaI ATCGAT 2 cut(s) 338, 587
CsiI ACCWGGT 1 cut(s) 691
Csp6I GTAC 4 cut(s) 177, 261, 696, 757
CviAII CATG 1 cut(s) 918
CviQI GTAC 4 cut(s) 177, 261, 696, 757
DdeI CTNAG 6 cut(s) 169, 498, 753, 872, 924, 1054
DinI GGCGCC 2 cut(s) 142, 1070
DpnI GATC 7 cut(s) 11, 337, 341, 586, 810, 877, 1112
DpnII GATC 7 cut(s) 9, 335, 339, 584, 808, 875, 1110
Eam1104I CTCTTC 4 cut(s) 121, 154, 662, 1074
EarI CTCTTC 4 cut(s) 121, 154, 662, 1074
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 521
Eco130I CCWWGG 1 cut(s) 268
Eco147I AGGCCT 1 cut(s) 542
Eco24I GRGCYC 1 cut(s) 523
Eco32I GATATC 1 cut(s) 1120
Eco53kI GAGCTC 1 cut(s) 521
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 2 cut(s) 93, 950
EcoICRI GAGCTC 1 cut(s) 521
EcoRII CCWGG 1 cut(s) 691
EcoRV GATATC 1 cut(s) 1120
EcoT14I CCWWGG 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 523
EgeI GGCGCC 2 cut(s) 142, 1070
EheI GGCGCC 2 cut(s) 142, 1070
ErhI CCWWGG 1 cut(s) 268
FaeI CATG 1 cut(s) 921
FaiI YATR 9 cut(s) 361, 531, 555, 564, 615, 644, 814, 897, 919
FalI AAGNNNNNCTT 3 cut(s) 527, 559, 1188
FatI CATG 1 cut(s) 917
Fnu4HI GCNGC 3 cut(s) 828, 893, 960
FokI GGATG 3 cut(s) 251, 443, 829
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 3 cut(s) 828, 893, 960
FspBI CTAG 1 cut(s) 576
GlaI GCGC 3 cut(s) 142, 240, 1070
GluI GCNGC 3 cut(s) 828, 893, 960
GsuI CTGGAG 2 cut(s) 16, 801
HaeII RGCGCY 3 cut(s) 144, 242, 1072
HaeIII GGCC 4 cut(s) 161, 542, 678, 1051
HapII CCGG 1 cut(s) 144
HhaI GCGC 3 cut(s) 143, 241, 1071
Hin1I GRCGYC 2 cut(s) 141, 1069
Hin1II CATG 1 cut(s) 921
Hin6I GCGC 3 cut(s) 141, 239, 1069
HinP1I GCGC 3 cut(s) 141, 239, 1069
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 3 cut(s) 96, 632, 848
HpaII CCGG 1 cut(s) 144
Hpy166II GTNNAC 2 cut(s) 911, 1171
Hpy188I TCNGA 7 cut(s) 253, 304, 466, 584, 880, 987, 1117
Hpy188III TCNNGA 3 cut(s) 33, 86, 524
Hpy8I GTNNAC 2 cut(s) 911, 1171
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 6 cut(s) 286, 383, 437, 532, 1069, 1190
HpyCH4III ACNGT 2 cut(s) 444, 901
HpyCH4IV ACGT 2 cut(s) 558, 793
HpyCH4V TGCA 9 cut(s) 4, 221, 316, 381, 421, 477, 592, 688, 905
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 483
HpyF3I CTNAG 6 cut(s) 169, 498, 753, 872, 924, 1054
HpySE526I ACGT 2 cut(s) 558, 793
Hsp92I GRCGYC 2 cut(s) 141, 1069
Hsp92II CATG 1 cut(s) 921
HspAI GCGC 3 cut(s) 141, 239, 1069
KasI GGCGCC 2 cut(s) 140, 1068
KroI GCCGGC 1 cut(s) 143
KroNI GCCGGC 1 cut(s) 145
Kzo9I GATC 7 cut(s) 9, 335, 339, 584, 808, 875, 1110
LguI GCTCTTC 1 cut(s) 154
LmnI GCTCC 4 cut(s) 112, 295, 518, 577
Lsp1109I GCAGC 3 cut(s) 814, 904, 946
LweI GCATC 4 cut(s) 79, 465, 579, 588
MabI ACCWGGT 1 cut(s) 691
MaeI CTAG 1 cut(s) 576
MaeII ACGT 2 cut(s) 558, 793
MaeIII GTNAC 2 cut(s) 493, 722
MalI GATC 7 cut(s) 11, 337, 341, 586, 810, 877, 1112
MboI GATC 7 cut(s) 9, 335, 339, 584, 808, 875, 1110
MboII GAAGA 7 cut(s) 108, 141, 240, 443, 444, 679, 1091
MflI RGATCY 1 cut(s) 875
MhlI GDGCHC 1 cut(s) 523
MluCI AATT 6 cut(s) 74, 376, 472, 736, 821, 881
Mly113I GGCGCC 2 cut(s) 141, 1069
MlyI GAGTC 1 cut(s) 105
MnlI CCTC 7 cut(s) 146, 277, 484, 663, 946, 1051, 1075
MroNI GCCGGC 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 1200
MseI TTAA 4 cut(s) 324, 330, 399, 471
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 1 cut(s) 693
MvaI CCWGG 1 cut(s) 693
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 2 cut(s) 180, 483
NaeI GCCGGC 1 cut(s) 145
NarI GGCGCC 2 cut(s) 141, 1069
NdeII GATC 7 cut(s) 9, 335, 339, 584, 808, 875, 1110
NgoMIV GCCGGC 1 cut(s) 143
NlaIII CATG 1 cut(s) 921
NlaIV GGNNCC 5 cut(s) 134, 142, 277, 573, 1070
NmeAIII GCCGAG 2 cut(s) 117, 512
NspV TTCGAA 1 cut(s) 430
PceI AGGCCT 1 cut(s) 542
PciSI GCTCTTC 1 cut(s) 154
PdiI GCCGGC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 1200
PfeI GAWTC 2 cut(s) 632, 848
PflMI CCANNNNNTGG 1 cut(s) 603
PkrI GCNGC 3 cut(s) 829, 894, 961
Ple19I CGATCG 1 cut(s) 338
PleI GAGTC 1 cut(s) 104
PluTI GGCGCC 2 cut(s) 144, 1072
PpsI GAGTC 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 691
PspGI CCWGG 1 cut(s) 691
PspN4I GGNNCC 5 cut(s) 134, 142, 277, 573, 1070
PspPI GGNCC 1 cut(s) 1050
PstNI CAGNNNCTG 1 cut(s) 287
PsuI RGATCY 1 cut(s) 875
PvuI CGATCG 1 cut(s) 338
RsaI GTAC 4 cut(s) 178, 262, 697, 758
RsaNI GTAC 4 cut(s) 177, 261, 696, 757
SacI GAGCTC 1 cut(s) 523
SapI GCTCTTC 1 cut(s) 154
SaqAI TTAA 4 cut(s) 324, 330, 399, 471
SatI GCNGC 3 cut(s) 828, 893, 960
Sau3AI GATC 7 cut(s) 9, 335, 339, 584, 808, 875, 1110
Sau96I GGNCC 1 cut(s) 1050
ScaI AGTACT 2 cut(s) 178, 758
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 1 cut(s) 693
SduI GDGCHC 1 cut(s) 523
SexAI ACCWGGT 1 cut(s) 691
SfaNI GCATC 4 cut(s) 79, 465, 579, 588
SfoI GGCGCC 2 cut(s) 142, 1070
SfuI TTCGAA 1 cut(s) 430
Sse9I AATT 6 cut(s) 74, 376, 472, 736, 821, 881
SseBI AGGCCT 1 cut(s) 542
SsiI CCGC 2 cut(s) 130, 183
SspDI GGCGCC 2 cut(s) 140, 1068
SspI AATATT 1 cut(s) 328
SspMI CTAG 1 cut(s) 576
SstI GAGCTC 1 cut(s) 523
StuI AGGCCT 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 691
StyI CCWWGG 1 cut(s) 268
TaaI ACNGT 2 cut(s) 444, 901
TaiI ACGT 2 cut(s) 561, 796
TaqI TCGA 5 cut(s) 102, 338, 430, 587, 807
TasI AATT 6 cut(s) 74, 376, 472, 736, 821, 881
TatI WGTACW 2 cut(s) 176, 756
TfiI GAWTC 2 cut(s) 632, 848
Tru1I TTAA 4 cut(s) 324, 330, 399, 471
Tru9I TTAA 4 cut(s) 324, 330, 399, 471
TscAI CASTG 1 cut(s) 228
TseI GCWGC 3 cut(s) 827, 892, 959
TspDTI ATGAA 3 cut(s) 671, 902, 906
TspRI CASTG 1 cut(s) 228
Van91I CCANNNNNTGG 1 cut(s) 603
XapI RAATTY 1 cut(s) 821
XmnI GAANNNNTTC 1 cut(s) 1200
XspI CTAG 1 cut(s) 576
ZrmI AGTACT 2 cut(s) 178, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.