Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004198

Physical Location & Seq

Genomic Coordinates Reverse (-)

18101949 .. 18105142

Gene Structure 3194 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1200300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1011 bp

ATGCTCGCCACTCTCAAGGCCGAAGAGCAGAGCCAGCAGCTGCAGCTCGTCGAGCGCGAGGACATTGAGGATGAAGAGGACCTGTTCGAAGCGATCGACAAGTTGATTGCTCAGGGAATCAATGCCGGAGATATTAAGAAGCTTCAGGATGCAGGGATCTACACCTGCAATGGCTTGATGATGCATACGAAGAAGAACTTGACTGGAATCAAAGGATTATCAGAGGCAAAAGTTGATAAGATATGCGAAGCTGCTGAAAAGATAGTGAACTTTGGATATATTACTGGAAGTGACGCTCTGATCAAAAGAAAGTCGGTGATTAAGATTACAACCGGAAGCCATGCCCTTGATGAACTTCTAGGCGGTGGAGTTGAAACTATGTCAATCACGGAAGCGTTTGGAGAATTCCGGTGTGGGAAAACACAGCTTGCTCATACTCTCTGCGTTTCTACACAGCTTCCCACCAACATGAAGGGAGGGAATGGAAAAGTTGCTTACATTGATACTGAAGGAACCTTCCGACCTGATCGAATTGTGCCAATAGCTGAAAGATTTGGCTTGGATCCAGGAGCTGTCCTGGACAATATCATTTATGCTCGTGCATATACATATGAGCATCAGTATAACCTGCTTCTTGGTCTGGCTGCAAAAATGTCTGAAGAGCCATTCAGACTTCTGATTGTGGATTCGGTGATTGCCCTCTTTCGAGTTGACTTTACAGGACGAGGAGAGCTTGCAGACCGTCAGCAAAAACTAGCTCAGATGCTTTCCCGACTAACGAAGATAGCTGAGGAGTTCAACGTTGCTGTCTACATGACCAACCAAGTGATAGCCGACCCTGGTGGCGGAGTGTTCATATCAGACCCAAAGAAACCAGCAGGAGGACATGTGCTTGCCCATGCTGCAACAATCAGGCTGATGTTCAGGAAAGGCAAAGGCGAACAGCGTGTCTGCAAGGTGTTTGATGCCCCAAACCTGCCCGAGGCTGAAGCAATATCCTTCACAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000150 GO:0000217 GO:0000228 GO:0000280 GO:0000400 GO:0000724 GO:0000725 GO:0000730 GO:0000793 GO:0000794 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0033554 GO:0034622 GO:0034641 GO:0035825 GO:0042148 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045003 GO:0045132 GO:0045143 GO:0046483 GO:0048285 GO:0050896 GO:0051026 GO:0051276 GO:0051321 GO:0051716 GO:0061982 GO:0065003 GO:0065004 GO:0070013 GO:0070192 GO:0071704 GO:0071824 GO:0071840 GO:0090304 GO:0090735 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

Rad51

Protein Analysis

337

Amino Acids

36.8

Weight (kDa)

5.56

Isoelectric Point (pI)

25.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad51	PF08423	102 - 332	2.3e-118	Rad51
RecA_N	PF00154	103 - 311	3.8e-11	RecA N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004198)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22880 AT3G22880
fragaria_vesca	FvH4_4g03620 FvH4_4g03620 FvH4_4g03740 FvH4_4g03740 FvH4_4g03740
malus_domestica	MD16G1200300.v1.1
prunus_persica	Prupe.1G022500_v2.0.a1 Prupe.1G022500_v2.0.a1
pyrus_communis	pycom16g16950
rosa_chinensis	RchiOBHm_Chr4g0392991 RchiOBHm_Chr4g0393441 RchiOBHm_Chr4g0393481
rosa_laevigata	RLG00000009753 RLG00000009756 RLG00000009776 RLG00000010122
rosa_multiflora	Rmu_sc0008339.1_g000015 Rmu_sc0008931.1_g000008 Rmu_sc0012965.1_g000002
rosa_roxburghii	Rroxscaffold_5G00338100 Rroxscaffold_5G00338400
rosa_rugosa	Rorug03G0309300 Rorug03G0310500 Rorug04G0001200 Rorug04G0001300 Rorug04G0001700 Rorug04G0003100
rosa_samantha	Rh4CG016900
rosa_wichuraiana	Rw4G003560 Rw4G003710 Rw4G003740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	173
Acc36I	ACCTGC	3 cut(s)	173, 636, 984
AccI	GTMKAC	1 cut(s)	810
AccII	CGCG	1 cut(s)	57
AciI	CCGC	2 cut(s)	363, 846
AclI	AACGTT	1 cut(s)	801
AclWI	GGATC	3 cut(s)	164, 557, 570
AcsI	RAATTY	1 cut(s)	404
AcuI	CTGAAG	4 cut(s)	128, 528, 678, 1008
AfiI	CCNNNNNNNGG	3 cut(s)	845, 881, 982
AflIII	ACRYGT	1 cut(s)	886
AgsI	TTSAA	2 cut(s)	374, 799
AjnI	CCWGG	3 cut(s)	565, 576, 838
AlwI	GGATC	3 cut(s)	164, 557, 570
AlwNI	CAGNNNCTG	2 cut(s)	40, 572
Ama87I	CYCGRG	1 cut(s)	980
AoxI	GGCC	1 cut(s)	18
ApeKI	GCWGC	6 cut(s)	37, 40, 43, 251, 644, 902
ApoI	RAATTY	1 cut(s)	404
ArsI	GACNNNNNNTTYG	2 cut(s)	933, 965
AspLEI	GCGC	1 cut(s)	57
AspS9I	GGNCC	1 cut(s)	79
AsuHPI	GGTGA	2 cut(s)	328, 703
AsuII	TTCGAA	1 cut(s)	87
AvaI	CYCGRG	1 cut(s)	980
AvaII	GGWCC	1 cut(s)	79
BamHI	GGATCC	1 cut(s)	562
BauI	CACGAG	1 cut(s)	597
BbvCI	CCTCAGC	1 cut(s)	789
BbvI	GCAGC	6 cut(s)	27, 49, 55, 238, 631, 889
BciT130I	CCWGG	3 cut(s)	567, 578, 840
BclI	TGATCA	1 cut(s)	300
BfaI	CTAG	2 cut(s)	359, 755
BfmI	CTRYAG	1 cut(s)	41
BfuAI	ACCTGC	3 cut(s)	173, 636, 984
BisI	GCNGC	6 cut(s)	38, 41, 44, 252, 645, 903
BlsI	GCNGC	6 cut(s)	39, 42, 45, 253, 646, 904
Bme1390I	CCNGG	3 cut(s)	567, 578, 840
Bme18I	GGWCC	1 cut(s)	79
BmeT110I	CYCGRG	1 cut(s)	980
BmgT120I	GGNCC	1 cut(s)	79
BmiI	GGNNCC	2 cut(s)	514, 564
BmrFI	CCNGG	3 cut(s)	567, 578, 840
BmsI	GCATC	5 cut(s)	139, 171, 625, 753, 955
Bpu10I	CCTNAGC	2 cut(s)	111, 789
Bpu14I	TTCGAA	1 cut(s)	87
BsaJI	CCNNGG	2 cut(s)	838, 981
BsaWI	WCCGGW	2 cut(s)	332, 408
BsaXI	ACNNNNNCTCC	2 cut(s)	873, 903
Bsc4I	CCNNNNNNNGG	3 cut(s)	845, 881, 982
Bse1I	ACTGG	2 cut(s)	208, 289
Bse3DI	GCAATG	1 cut(s)	175
BseBI	CCWGG	3 cut(s)	567, 578, 840
BseDI	CCNNGG	2 cut(s)	838, 981
BseGI	GGATG	2 cut(s)	76, 154
BseLI	CCNNNNNNNGG	3 cut(s)	845, 881, 982
BseMI	GCAATG	1 cut(s)	175
BseMII	CTCAG	3 cut(s)	125, 773, 780
BseNI	ACTGG	2 cut(s)	208, 289
BseRI	GAGGAG	2 cut(s)	741, 806
BseXI	GCAGC	6 cut(s)	27, 49, 55, 238, 631, 889
Bsh1236I	CGCG	1 cut(s)	57
Bsh1285I	CGRYCG	1 cut(s)	96
BshFI	GGCC	1 cut(s)	20
BsiEI	CGRYCG	1 cut(s)	96
BsiHKCI	CYCGRG	1 cut(s)	980
BsiSI	CCGG	3 cut(s)	126, 333, 409
BslI	CCNNNNNNNGG	3 cut(s)	845, 881, 982
BsnI	GGCC	1 cut(s)	20
BsoBI	CYCGRG	1 cut(s)	980
Bsp119I	TTCGAA	1 cut(s)	87
Bsp143I	GATC	5 cut(s)	93, 156, 300, 526, 562
BspACI	CCGC	2 cut(s)	363, 846
BspANI	GGCC	1 cut(s)	20
BspCNI	CTCAG	3 cut(s)	124, 772, 781
BspFNI	CGCG	1 cut(s)	57
BspLI	GGNNCC	2 cut(s)	514, 564
BspMAI	CTGCAG	1 cut(s)	45
BspMI	ACCTGC	3 cut(s)	173, 636, 984
BspPI	GGATC	3 cut(s)	164, 557, 570
BspQI	GCTCTTC	2 cut(s)	18, 654
BspT104I	TTCGAA	1 cut(s)	87
BsrDI	GCAATG	1 cut(s)	175
BsrI	ACTGG	2 cut(s)	208, 289
BssECI	CCNNGG	2 cut(s)	838, 981
BssMI	GATC	5 cut(s)	93, 156, 300, 526, 562
BssSI	CACGAG	1 cut(s)	597
Bst2BI	CACGAG	1 cut(s)	597
Bst2UI	CCWGG	3 cut(s)	567, 578, 840
Bst4CI	ACNGT	1 cut(s)	743
Bst6I	CTCTTC	3 cut(s)	18, 69, 654
BstBI	TTCGAA	1 cut(s)	87
BstC8I	GCNNGC	5 cut(s)	6, 35, 429, 735, 894
BstDEI	CTNAG	3 cut(s)	111, 759, 789
BstENI	CCTNNNNNAGG	1 cut(s)	980
BstF5I	GGATG	2 cut(s)	76, 154
BstFNI	CGCG	1 cut(s)	57
BstHHI	GCGC	1 cut(s)	57
BstKTI	GATC	5 cut(s)	96, 159, 303, 529, 565
BstMBI	GATC	5 cut(s)	93, 156, 300, 526, 562
BstMCI	CGRYCG	1 cut(s)	96
BstMWI	GCNNNNNNNGC	4 cut(s)	34, 43, 52, 902
BstNI	CCWGG	3 cut(s)	567, 578, 840
BstNSI	RCATGY	1 cut(s)	890
BstSCI	CCNGG	3 cut(s)	565, 576, 838
BstSFI	CTRYAG	1 cut(s)	41
BstUI	CGCG	1 cut(s)	57
BstV1I	GCAGC	6 cut(s)	27, 49, 55, 238, 631, 889
BstX2I	RGATCY	2 cut(s)	156, 562
BstYI	RGATCY	2 cut(s)	156, 562
BsuRI	GGCC	1 cut(s)	20
BtsCI	GGATG	2 cut(s)	76, 154
BveI	ACCTGC	3 cut(s)	173, 636, 984
Cac8I	GCNNGC	5 cut(s)	6, 35, 429, 735, 894
CaiI	CAGNNNCTG	2 cut(s)	40, 572
CfoI	GCGC	1 cut(s)	57
Cfr13I	GGNCC	1 cut(s)	79
CseI	GACGC	1 cut(s)	302
CviAII	CATG	5 cut(s)	341, 469, 814, 887, 899
DdeI	CTNAG	3 cut(s)	111, 759, 789
DpnI	GATC	5 cut(s)	95, 158, 302, 528, 564
DpnII	GATC	5 cut(s)	93, 156, 300, 526, 562
Eam1104I	CTCTTC	3 cut(s)	18, 69, 654
EarI	CTCTTC	3 cut(s)	18, 69, 654
EciI	GGCGGA	1 cut(s)	861
Eco47I	GGWCC	1 cut(s)	79
Eco57I	CTGAAG	4 cut(s)	128, 528, 678, 1008
Eco88I	CYCGRG	1 cut(s)	980
EcoNI	CCTNNNNNAGG	1 cut(s)	980
EcoO109I	RGGNCCY	1 cut(s)	79
EcoRI	GAATTC	1 cut(s)	404
EcoRII	CCWGG	3 cut(s)	565, 576, 838
EcoT22I	ATGCAT	1 cut(s)	186
FaeI	CATG	5 cut(s)	344, 472, 817, 890, 902
FalI	AAGNNNNNCTT	2 cut(s)	182, 214
FatI	CATG	5 cut(s)	340, 468, 813, 886, 898
FauNDI	CATATG	1 cut(s)	610
FbaI	TGATCA	1 cut(s)	300
FblI	GTMKAC	1 cut(s)	810
Fnu4HI	GCNGC	6 cut(s)	38, 41, 44, 252, 645, 903
FokI	GGATG	2 cut(s)	83, 161
Fsp4HI	GCNGC	6 cut(s)	38, 41, 44, 252, 645, 903
FspBI	CTAG	2 cut(s)	359, 755
GlaI	GCGC	1 cut(s)	56
GluI	GCNGC	6 cut(s)	38, 41, 44, 252, 645, 903
HaeIII	GGCC	1 cut(s)	20
HapII	CCGG	3 cut(s)	126, 333, 409
HgaI	GACGC	1 cut(s)	302
HhaI	GCGC	1 cut(s)	57
Hin1II	CATG	5 cut(s)	344, 472, 817, 890, 902
Hin6I	GCGC	1 cut(s)	55
HinP1I	GCGC	1 cut(s)	55
HincII	GTYRAC	1 cut(s)	712
HindII	GTYRAC	1 cut(s)	712
HindIII	AAGCTT	1 cut(s)	140
HinfI	GANTC	3 cut(s)	117, 207, 686
HpaII	CCGG	3 cut(s)	126, 333, 409
HphI	GGTGA	2 cut(s)	328, 703
Hpy166II	GTNNAC	3 cut(s)	268, 712, 811
Hpy188I	TCNGA	8 cut(s)	223, 300, 521, 658, 671, 678, 762, 862
Hpy188III	TCNNGA	3 cut(s)	146, 771, 925
Hpy8I	GTNNAC	3 cut(s)	268, 712, 811
Hpy99I	CGWCG	1 cut(s)	53
HpyAV	CCTTC	4 cut(s)	466, 503, 526, 1009
HpyCH4III	ACNGT	1 cut(s)	743
HpyCH4IV	ACGT	1 cut(s)	801
HpyCH4V	TGCA	9 cut(s)	43, 152, 168, 184, 602, 647, 737, 905, 954
HpyF10VI	GCNNNNNNNGC	4 cut(s)	34, 43, 52, 902
HpyF3I	CTNAG	3 cut(s)	111, 759, 789
HpySE526I	ACGT	1 cut(s)	801
Hsp92II	CATG	5 cut(s)	344, 472, 817, 890, 902
HspAI	GCGC	1 cut(s)	55
Ksp22I	TGATCA	1 cut(s)	300
Kzo9I	GATC	5 cut(s)	93, 156, 300, 526, 562
LguI	GCTCTTC	2 cut(s)	18, 654
LmnI	GCTCC	1 cut(s)	569
Lsp1109I	GCAGC	6 cut(s)	27, 49, 55, 238, 631, 889
LweI	GCATC	5 cut(s)	139, 171, 625, 753, 955
MaeI	CTAG	2 cut(s)	359, 755
MaeII	ACGT	1 cut(s)	801
MaeIII	GTNAC	1 cut(s)	290
MalI	GATC	5 cut(s)	95, 158, 302, 528, 564
MboI	GATC	5 cut(s)	93, 156, 300, 526, 562
MboII	GAAGA	6 cut(s)	35, 86, 202, 205, 671, 793
MflI	RGATCY	2 cut(s)	156, 562
MluCI	AATT	2 cut(s)	404, 531
MmeI	TCCRAC	1 cut(s)	544
Mph1103I	ATGCAT	1 cut(s)	186
MseI	TTAA	2 cut(s)	135, 321
MslI	CAYNNNNRTG	1 cut(s)	467
MspA1I	CMGCKG	1 cut(s)	40
MspI	CCGG	3 cut(s)	126, 333, 409
MspR9I	CCNGG	3 cut(s)	567, 578, 840
MvaI	CCWGG	3 cut(s)	567, 578, 840
MvnI	CGCG	1 cut(s)	57
MwoI	GCNNNNNNNGC	4 cut(s)	34, 43, 52, 902
NdeI	CATATG	1 cut(s)	610
NdeII	GATC	5 cut(s)	93, 156, 300, 526, 562
NlaIII	CATG	5 cut(s)	344, 472, 817, 890, 902
NlaIV	GGNNCC	2 cut(s)	514, 564
NmuCI	GTSAC	1 cut(s)	290
NsiI	ATGCAT	1 cut(s)	186
NspI	RCATGY	1 cut(s)	890
NspV	TTCGAA	1 cut(s)	87
PaqCI	CACCTGC	1 cut(s)	173
PciI	ACATGT	1 cut(s)	886
PciSI	GCTCTTC	2 cut(s)	18, 654
PcsI	WCGNNNNNNNCGW	2 cut(s)	54, 93
PfeI	GAWTC	3 cut(s)	117, 207, 686
PfoI	TCCNGGA	2 cut(s)	565, 576
PkrI	GCNGC	6 cut(s)	39, 42, 45, 253, 646, 904
Ple19I	CGATCG	1 cut(s)	96
PpuMI	RGGWCCY	1 cut(s)	79
PscI	ACATGT	1 cut(s)	886
Psp1406I	AACGTT	1 cut(s)	801
Psp5II	RGGWCCY	1 cut(s)	79
Psp6I	CCWGG	3 cut(s)	565, 576, 838
PspGI	CCWGG	3 cut(s)	565, 576, 838
PspN4I	GGNNCC	2 cut(s)	514, 564
PspPI	GGNCC	1 cut(s)	79
PspPPI	RGGWCCY	1 cut(s)	79
PstI	CTGCAG	1 cut(s)	45
PstNI	CAGNNNCTG	2 cut(s)	40, 572
PsuI	RGATCY	2 cut(s)	156, 562
PvuI	CGATCG	1 cut(s)	96
PvuII	CAGCTG	1 cut(s)	40
RseI	CAYNNNNRTG	1 cut(s)	467
SapI	GCTCTTC	2 cut(s)	18, 654
SaqAI	TTAA	2 cut(s)	135, 321
SatI	GCNGC	6 cut(s)	38, 41, 44, 252, 645, 903
Sau3AI	GATC	5 cut(s)	93, 156, 300, 526, 562
Sau96I	GGNCC	1 cut(s)	79
ScrFI	CCNGG	3 cut(s)	567, 578, 840
SfaNI	GCATC	5 cut(s)	139, 171, 625, 753, 955
SfcI	CTRYAG	1 cut(s)	41
SfuI	TTCGAA	1 cut(s)	87
SinI	GGWCC	1 cut(s)	79
SmiMI	CAYNNNNRTG	1 cut(s)	467
SmlI	CTYRAG	1 cut(s)	14
SmoI	CTYRAG	1 cut(s)	14
Sse9I	AATT	2 cut(s)	404, 531
SsiI	CCGC	2 cut(s)	363, 846
SspMI	CTAG	2 cut(s)	359, 755
StyD4I	CCNGG	3 cut(s)	565, 576, 838
TaaI	ACNGT	1 cut(s)	743
TaiI	ACGT	1 cut(s)	804
TaqI	TCGA	5 cut(s)	51, 87, 96, 529, 706
TasI	AATT	2 cut(s)	404, 531
TfiI	GAWTC	3 cut(s)	117, 207, 686
Tru1I	TTAA	2 cut(s)	135, 321
Tru9I	TTAA	2 cut(s)	135, 321
TseFI	GTSAC	1 cut(s)	290
TseI	GCWGC	6 cut(s)	37, 40, 43, 251, 644, 902
Tsp45I	GTSAC	1 cut(s)	290
TspDTI	ATGAA	4 cut(s)	87, 366, 485, 844
TspGWI	ACGGA	1 cut(s)	404
VpaK11BI	GGWCC	1 cut(s)	79
XagI	CCTNNNNNAGG	1 cut(s)	980
XapI	RAATTY	1 cut(s)	404
XceI	RCATGY	1 cut(s)	890
XmiI	GTMKAC	1 cut(s)	810
XspI	CTAG	2 cut(s)	359, 755
Zsp2I	ATGCAT	1 cut(s)	186

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.