MD16G1246300.v1.1

SRP40, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008781
Physical Location & Seq
Reverse (-)
27246423 .. 27252166
5744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1246300.v1.1.491

Sequence Viewer

Length: 1353 bp
ATGCCCAAATCCCTAACAAGCACGCAGGAGGACGCTATCAACCCTACCCTTCTCGCATTCAAGCCTCGCCAAGTTCTGCTCGCAAACCGAGACATGGAGCACAGGAAGCCCAATAGCTCTGCAGACGGAGCACAAACTCCGAGTCCCGACCAGAAGGGTCCACTCCTCCGCGCCGTGGCTCGGTTCCTGGAAAACAATGGGTTCTCCAAAACCTTGAAAAAGTTCATTTCTGAAGCTGGAATCGAGAAAAGTGAACTGAAGGATTTGCCACTGGATTTGGGAGAAATGTACTGCAAGTATTCTGAGATGTGTAGTGGAGGCACAAATGTCCACAGCGAGGAGAAAGTATTGAAGGATACCTTCCAATTGCTGTTAATGGCGGATAAGTTATCCAAGGAGGCAAAGAAGTCCAAGCTAGCTTCTGGTTCTCTTGCTGGTGCAGCAGAAGAACATCAATCAGAGCAATTTCTTGGTGCAACTGAAAAAAAAATTAAAGATGCTGTACTTTTAAAGGAAAATGGTGTGGGTGATTCTGAGATAGAAAAGAAACCAAGGGATAAGAAGAAAAAGAAGAACAAGTCAAGTACCGATTCTGTCAGTGCTGGTGTAGAGCAGCATGGCTTGGAAGGAAAAAATGATGCGATTGAAATGGGTAAATCAGTTGCTGATGACAATCCAATGGATGGGAAGAGTGTCAAACCTAAAAGTAAGAAGAAAAAGAAAGATGGTTTGGTTTCTGAAAGTTTAGGTGGTGAGAAAGGGAAGGCGCTTAGTATAGGAGATACGAATGGAACTAGTTCTGGAAAATATGATTTCAAGATATCAGATGTGGATGCTACTGATAAGGAGAATAAAGGTTCCAAAAAAAGAAAAAGATTAGCTTCTGAAGGATATGATTCACAGCCTGCCAACAACAAAGAAGATGAAGTATCCAACCGCAGAAAGGTAGAGGGCTCAAAAGCATCCAAGGGAAGTGAGCAACCAGTAAATAACAATGCATCACAAGGAAAAGCTGAAAATTTTGGTGAACTAGATAAAAGTGCTAAGAAGTCTTTTATTAAGAAAAACAAGAAACAACACAATGGTTCAGCTGAGCCAAAGACTGTTAATGCATTTCAAAGGGTAAAAGCTGATGAGGTGGAATTTATTGATGAGAAGCTTCGAGATAATTCTTACTGGGCAAAGGATGGTGCGGAGATTGGCTATGGTGCAAAAGCACAAGAGATTCTTGGGCAAGTCAGAGGAAGGGATTTTCGGCATGAAAAGACCAAGAAGAAGCGTGGGACATACAGAGGAGGGCTGATTGATCTGCAGTCACACTCGGTCAAGTTCAATTATTCTGATGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRP40_C

Protein Analysis

451

Amino Acids

49.38

Weight (kDa)

9.29

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP40_C PF05022 372 - 444 3e-27 SRP40, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 683
AccII CGCG 1 cut(s) 171
AciI CCGC 4 cut(s) 169, 380, 937, 1193
AcsI RAATTY 2 cut(s) 1018, 1142
AcuI CTGAAG 3 cut(s) 252, 278, 906
AfaI GTAC 3 cut(s) 290, 504, 586
AfiI CCNNNNNNNGG 6 cut(s) 94, 175, 180, 337, 683, 943
AgsI TTSAA 7 cut(s) 61, 217, 352, 647, 817, 1118, 1333
AhlI ACTAGT 1 cut(s) 794
AjnI CCWGG 1 cut(s) 186
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
AluBI AGCT 9 cut(s) 117, 236, 415, 419, 881, 1013, 1091, 1130, 1159
AluI AGCT 9 cut(s) 117, 236, 415, 419, 881, 1013, 1091, 1130, 1159
Alw21I GWGCWC 2 cut(s) 102, 133
Alw26I GTCTC 1 cut(s) 84
AlwNI CAGNNNCTG 1 cut(s) 665
ApeKI GCWGC 2 cut(s) 440, 613
ApoI RAATTY 2 cut(s) 1018, 1142
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
Asp700I GAANNNNTTC 2 cut(s) 221, 796
AspLEI GCGC 2 cut(s) 173, 769
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 539, 764, 1037
AsuNHI GCTAGC 1 cut(s) 415
AvaII GGWCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 956
Bbv12I GWGCWC 2 cut(s) 102, 133
BbvI GCAGC 2 cut(s) 452, 625
BccI CCATC 3 cut(s) 677, 719, 1181
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 188
BciVI GTATCC 2 cut(s) 349, 940
BcoDI GTCTC 1 cut(s) 84
BcuI ACTAGT 1 cut(s) 794
BfaI CTAG 3 cut(s) 416, 795, 1031
BfmI CTRYAG 2 cut(s) 120, 1310
BfoI RGCGCY 1 cut(s) 770
BfuI GTATCC 2 cut(s) 349, 940
BisI GCNGC 2 cut(s) 441, 614
BlpI GCTNAGC 1 cut(s) 1092
BlsI GCNGC 2 cut(s) 442, 615
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 3 cut(s) 159, 185, 859
BmrFI CCNGG 1 cut(s) 188
BmrI ACTGGG 1 cut(s) 1186
BmsI GCATC 5 cut(s) 487, 628, 823, 971, 1007
BmtI GCTAGC 1 cut(s) 419
BmuI ACTGGG 1 cut(s) 1186
Bpu1102I GCTNAGC 1 cut(s) 1092
BsaBI GATNNNNATC 1 cut(s) 672
BsaJI CCNNGG 4 cut(s) 174, 393, 551, 966
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 175, 180, 337, 683, 943
Bse1I ACTGG 3 cut(s) 276, 983, 1181
Bse8I GATNNNNATC 1 cut(s) 672
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 4 cut(s) 174, 393, 551, 966
BseGI GGATG 4 cut(s) 688, 838, 962, 1192
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 6 cut(s) 94, 175, 180, 337, 683, 943
BseMII CTCAG 3 cut(s) 294, 525, 1083
BseNI ACTGG 3 cut(s) 276, 983, 1181
BseRI GAGGAG 3 cut(s) 155, 353, 1308
BseXI GCAGC 2 cut(s) 452, 625
BsgI GTGCAG 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 171
BsiHKAI GWGCWC 2 cut(s) 102, 133
BslFI GGGAC 2 cut(s) 129, 1297
BslI CCNNNNNNNGG 6 cut(s) 94, 175, 180, 337, 683, 943
BsmAI GTCTC 1 cut(s) 84
BsmFI GGGAC 2 cut(s) 129, 1297
BsmI GAATGC 1 cut(s) 56
Bsp1286I GDGCHC 3 cut(s) 102, 133, 956
Bsp143I GATC 1 cut(s) 1306
Bsp1720I GCTNAGC 1 cut(s) 1092
BspACI CCGC 4 cut(s) 169, 380, 937, 1193
BspCNI CTCAG 3 cut(s) 295, 526, 1084
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 3 cut(s) 159, 185, 859
BspMAI CTGCAG 2 cut(s) 124, 1314
BspOI GCTAGC 1 cut(s) 419
BsrI ACTGG 3 cut(s) 276, 983, 1181
BssECI CCNNGG 4 cut(s) 174, 393, 551, 966
BssMI GATC 1 cut(s) 1306
BssT1I CCWWGG 3 cut(s) 393, 551, 966
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 1105
Bst6I CTCTTC 1 cut(s) 683
BstC8I GCNNGC 4 cut(s) 23, 81, 417, 906
BstDEI CTNAG 5 cut(s) 303, 534, 770, 1044, 1092
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 4 cut(s) 688, 838, 962, 1192
BstFNI CGCG 1 cut(s) 171
BstH2I RGCGCY 1 cut(s) 770
BstHHI GCGC 2 cut(s) 173, 769
BstKTI GATC 1 cut(s) 1309
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 1 cut(s) 1306
BstMWI GCNNNNNNNGC 3 cut(s) 106, 128, 440
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstSFI CTRYAG 2 cut(s) 120, 1310
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 452, 625
BsuI GTATCC 2 cut(s) 349, 940
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 4 cut(s) 688, 838, 962, 1192
BtsIMutI CAGTG 2 cut(s) 269, 604
Cac8I GCNNGC 4 cut(s) 23, 81, 417, 906
CaiI CAGNNNCTG 1 cut(s) 665
CfoI GCGC 2 cut(s) 173, 769
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 41
Csp6I GTAC 3 cut(s) 289, 503, 585
CspCI CAANNNNNGTGG 2 cut(s) 258, 293
CviAII CATG 3 cut(s) 94, 617, 1259
CviQI GTAC 3 cut(s) 289, 503, 585
DdeI CTNAG 5 cut(s) 303, 534, 770, 1044, 1092
DpnI GATC 1 cut(s) 1308
DpnII GATC 1 cut(s) 1306
DraI TTTAAA 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 683
EarI CTCTTC 1 cut(s) 683
EciI GGCGGA 1 cut(s) 395
Eco130I CCWWGG 3 cut(s) 393, 551, 966
Eco24I GRGCYC 1 cut(s) 956
Eco32I GATATC 1 cut(s) 822
Eco47I GGWCC 1 cut(s) 158
Eco57I CTGAAG 3 cut(s) 252, 278, 906
EcoRII CCWGG 1 cut(s) 186
EcoRV GATATC 1 cut(s) 822
EcoT14I CCWWGG 3 cut(s) 393, 551, 966
EcoT22I ATGCAT 2 cut(s) 1000, 1114
EcoT38I GRGCYC 1 cut(s) 956
ErhI CCWWGG 3 cut(s) 393, 551, 966
FaeI CATG 3 cut(s) 97, 620, 1262
FaiI YATR 8 cut(s) 95, 618, 776, 810, 894, 1206, 1260, 1288
FalI AAGNNNNNCTT 6 cut(s) 344, 376, 865, 897, 1212, 1244
FaqI GGGAC 2 cut(s) 129, 1297
FatI CATG 3 cut(s) 93, 616, 1258
Fnu4HI GCNGC 2 cut(s) 441, 614
FokI GGATG 4 cut(s) 695, 845, 949, 1199
FriOI GRGCYC 1 cut(s) 956
Fsp4HI GCNGC 2 cut(s) 441, 614
FspBI CTAG 3 cut(s) 416, 795, 1031
GlaI GCGC 2 cut(s) 172, 768
GluI GCNGC 2 cut(s) 441, 614
HaeII RGCGCY 1 cut(s) 770
HgaI GACGC 1 cut(s) 41
HhaI GCGC 2 cut(s) 173, 769
Hin1II CATG 3 cut(s) 97, 620, 1262
Hin6I GCGC 2 cut(s) 171, 767
HinP1I GCGC 2 cut(s) 171, 767
HindIII AAGCTT 1 cut(s) 1157
HinfI GANTC 6 cut(s) 142, 240, 530, 590, 896, 1225
HphI GGTGA 3 cut(s) 539, 764, 1037
Hpy166II GTNNAC 4 cut(s) 161, 254, 331, 1028
Hpy188III TCNNGA 5 cut(s) 146, 244, 801, 817, 1163
Hpy8I GTNNAC 4 cut(s) 161, 254, 331, 1028
HpyAV CCTTC 9 cut(s) 59, 148, 253, 346, 370, 620, 757, 881, 1239
HpyCH4III ACNGT 1 cut(s) 1105
HpyCH4V TGCA 8 cut(s) 122, 294, 440, 476, 998, 1112, 1211, 1312
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 128, 440
HpyF3I CTNAG 5 cut(s) 303, 534, 770, 1044, 1092
Hsp92II CATG 3 cut(s) 97, 620, 1262
HspAI GCGC 2 cut(s) 171, 767
Kzo9I GATC 1 cut(s) 1306
LmnI GCTCC 2 cut(s) 97, 128
Lsp1109I GCAGC 2 cut(s) 452, 625
LweI GCATC 5 cut(s) 487, 628, 823, 971, 1007
MaeI CTAG 3 cut(s) 416, 795, 1031
MaeIII GTNAC 1 cut(s) 1314
MalI GATC 1 cut(s) 1308
MboI GATC 1 cut(s) 1306
MboII GAAGA 7 cut(s) 458, 574, 583, 700, 724, 932, 1285
MfeI CAATTG 1 cut(s) 365
MhlI GDGCHC 3 cut(s) 102, 133, 956
MluCI AATT 7 cut(s) 365, 464, 489, 1018, 1142, 1168, 1333
MlyI GAGTC 1 cut(s) 151
MmeI TCCRAC 1 cut(s) 957
Mph1103I ATGCAT 2 cut(s) 1000, 1114
MroXI GAANNNNTTC 2 cut(s) 221, 796
MseI TTAA 6 cut(s) 374, 492, 509, 1059, 1107, 1351
MspA1I CMGCKG 1 cut(s) 1091
MspR9I CCNGG 1 cut(s) 188
MunI CAATTG 1 cut(s) 365
Mva1269I GAATGC 1 cut(s) 56
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 106, 128, 440
NdeII GATC 1 cut(s) 1306
NheI GCTAGC 1 cut(s) 415
NlaIII CATG 3 cut(s) 97, 620, 1262
NlaIV GGNNCC 3 cut(s) 159, 185, 859
NmuCI GTSAC 1 cut(s) 1314
NsiI ATGCAT 2 cut(s) 1000, 1114
PctI GAATGC 1 cut(s) 56
PdmI GAANNNNTTC 2 cut(s) 221, 796
PfeI GAWTC 5 cut(s) 240, 530, 590, 896, 1225
PflMI CCANNNNNTGG 1 cut(s) 683
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 2 cut(s) 442, 615
PleI GAGTC 1 cut(s) 150
PpsI GAGTC 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 3 cut(s) 159, 185, 859
PspPI GGNCC 1 cut(s) 158
PstI CTGCAG 2 cut(s) 124, 1314
PstNI CAGNNNCTG 1 cut(s) 665
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 3 cut(s) 290, 504, 586
RsaNI GTAC 3 cut(s) 289, 503, 585
SaqAI TTAA 6 cut(s) 374, 492, 509, 1059, 1107, 1351
SatI GCNGC 2 cut(s) 441, 614
Sau3AI GATC 1 cut(s) 1306
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 1 cut(s) 151
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 3 cut(s) 102, 133, 956
SfaNI GCATC 5 cut(s) 487, 628, 823, 971, 1007
SfcI CTRYAG 2 cut(s) 120, 1310
SinI GGWCC 1 cut(s) 158
SpeI ACTAGT 1 cut(s) 794
Sse9I AATT 7 cut(s) 365, 464, 489, 1018, 1142, 1168, 1333
SsiI CCGC 4 cut(s) 169, 380, 937, 1193
SspMI CTAG 3 cut(s) 416, 795, 1031
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 3 cut(s) 393, 551, 966
TaaI ACNGT 1 cut(s) 1105
TaqI TCGA 2 cut(s) 243, 1162
TaqII GACCGA 1 cut(s) 1312
TasI AATT 7 cut(s) 365, 464, 489, 1018, 1142, 1168, 1333
TatI WGTACW 2 cut(s) 288, 502
TfiI GAWTC 5 cut(s) 240, 530, 590, 896, 1225
Tru1I TTAA 6 cut(s) 374, 492, 509, 1059, 1107, 1351
Tru9I TTAA 6 cut(s) 374, 492, 509, 1059, 1107, 1351
TscAI CASTG 2 cut(s) 276, 604
TseFI GTSAC 1 cut(s) 1314
TseI GCWGC 2 cut(s) 440, 613
Tsp45I GTSAC 1 cut(s) 1314
TspDTI ATGAA 3 cut(s) 214, 939, 1275
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 2 cut(s) 276, 604
Van91I CCANNNNNTGG 1 cut(s) 683
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 2 cut(s) 1018, 1142
XmnI GAANNNNTTC 2 cut(s) 221, 796
XspI CTAG 3 cut(s) 416, 795, 1031
Zsp2I ATGCAT 2 cut(s) 1000, 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.