Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008553

Physical Location & Seq

Genomic Coordinates Reverse (-)

37922790 .. 37931541

Gene Structure 8752 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1279200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1230 bp

ATGGGTAGCAGTGAGATGGACAAACCCTCTAAAGAGAAAGAGAAGGAGGCAAAGACAACACCTGCTAGTACGCAGGAGCAGTCTGCGACTACCAGTGCTGGCACTGTTAATCCTGAGTGGTCGGGATTTCAGGCATATTCTCCAATACCTCCACATGGATTTATGGCCTCAAGTCCTCAAGCTCACCCGTATATGTGGGGGGTTCAGCATATTATGCCACCCTATGGTACCCCTCCCCACCCATATGTTGCAATGTATCCCCATGGCGGCTTATATGCTCACCCGTCTATGCCTCCTGGATCTTATCCTTTTAGCCCGTTTGCTATGCCTACTCCAAATGGTATTGTAGAGGTTTCTGGTAACATACCTGGAAGCATGGAAGCAGATGGTAAGCCATCTGAGGTCAAGGACAAATTACCAATAAAACGATCAAAAGGAAGCTTGGGCAGTTTGAATATGATTACAGGGAAGAATAACGAGCTTGGCAAAACATCAGGAGCCTCTGCTAATGGAATTTATTCCAAAAGTGCTGAGAGTGCTAGTGATGGTACAAGTGAAGGAAGTGATGCAAATTCTCAGAGTGACTCACAATTGAAGTCTGGGGTCAGGCAAGATTCTTTGGAAGGTAATGCATCTCAGAATGGCAGTTCTGCACATGGTTCTCAAAATGGAGCACCAAACACACATACCGTGTTGAATCAGACGATGGCCGTCATGCCAATAACGGCTGCTGGTGCTCCTGGCGTTGTTCCTGGTCCTGCAACTAACTTAAATATTGGGATGGACTATTGGGGAGCTCCACCTTCTGCCGCCATGCCTGCAATGCGTGGGAAGGTTCCAACTACTCCAGTATCAGCAGGAATAGTTACTGCTGGGTCTCGGGATAGTGTTCAGTCACAGCTTTGGTTACAAGATGAGAGAGAGCTTAAAAGACAGAAAAGAAAGCAGTCAAATAGGGAATCCGCTCGTCGATCTAGGTTGCGCAAGCAGGCGGAATGCGATGAGCTGGCCCAGCGTGCTGAAGCCTTAAAAGAGGAAAATGGCACTCTTAGATCAGAAGTTAACCGGATCAGGAGCAAGTATGAGCTGCTTCTCTCAGAGAATACCTCTCTAAAGGAGAGGCTTGGAGAAATTCCTGGTCACGAAGATGTACGGTCTGCCAGAAGTGAACCACATTTGAGCAATGACACTAAACAGATTGCACAAACAGAACGGCATGGCGGCCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFMR MFMR_assoc bZIP_1

Protein Analysis

410

Amino Acids

43.6

Weight (kDa)

7.84

Isoelectric Point (pI)

58.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFMR	PF07777	1 - 98	1.1e-29	G-box binding protein MFMR
MFMR_assoc	PF16596	137 - 273	5.2e-57	Disordered region downstream of MFMR
bZIP_1	PF00170	306 - 368	8.6e-20	bZIP transcription factor
bZIP_2	PF07716	308 - 356	2.2e-07	Basic region leucine zipper

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008553)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32150 AT2G35530
fragaria_vesca	FvH4_5g23200 FvH4_5g23200
malus_domestica	MD06G1026900.v1.1 MD16G1279200.v1.1
prunus_persica	Prupe.5G027000_v2.0.a1
pyrus_communis	pycom06g02210 pycom16g24960
rosa_chinensis	RchiOBHm_Chr7g0212131
rosa_laevigata	RLG00000002925
rosa_multiflora	Rmu_sc0002283.1_g000045 Rmu_sc0013167.1_g000003
rosa_roxburghii	Rroxscaffold_3G00247090
rosa_rugosa	Rorug07G0131200 Rorug07G0131200 Rorug07G0131300
rosa_samantha	Rh7AG264300 Rh7BG258200 Rh7CG280800 Rh7DG270700
rosa_wichuraiana	Rw7G022550 Rw7G022560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	70
Acc16I	TGCGCA	1 cut(s)	983
Acc36I	ACCTGC	1 cut(s)	70
Acc65I	GGTACC	1 cut(s)	227
AccB1I	GGYRCC	1 cut(s)	227
AccB7I	CCANNNNNTGG	1 cut(s)	224
AccBSI	CCGCTC	1 cut(s)	965
AciI	CCGC	5 cut(s)	267, 810, 963, 992, 1221
AclWI	GGATC	2 cut(s)	307, 1076
AcoI	YGGCCR	1 cut(s)	708
AcsI	RAATTY	3 cut(s)	513, 571, 1131
AcuI	CTGAAG	1 cut(s)	1041
AfaI	GTAC	4 cut(s)	70, 229, 550, 1152
AfiI	CCNNNNNNNGG	3 cut(s)	155, 224, 266
AgsI	TTSAA	3 cut(s)	454, 595, 697
AjnI	CCWGG	5 cut(s)	295, 367, 739, 751, 1135
AluBI	AGCT	8 cut(s)	182, 441, 481, 797, 901, 925, 1006, 1087
AluI	AGCT	8 cut(s)	182, 441, 481, 797, 901, 925, 1006, 1087
Alw21I	GWGCWC	3 cut(s)	676, 739, 799
Alw26I	GTCTC	1 cut(s)	882
AlwI	GGATC	2 cut(s)	307, 1076
Ama87I	CYCGRG	1 cut(s)	879
AoxI	GGCC	4 cut(s)	165, 708, 1008, 1222
ApeKI	GCWGC	2 cut(s)	728, 1087
ApoI	RAATTY	3 cut(s)	513, 571, 1131
Asp700I	GAANNNNTTC	1 cut(s)	517
Asp718I	GGTACC	1 cut(s)	227
AspLEI	GCGC	1 cut(s)	984
AspS9I	GGNCC	2 cut(s)	755, 1009
AsuHPI	GGTGA	2 cut(s)	176, 272
AvaI	CYCGRG	1 cut(s)	879
AvaII	GGWCC	1 cut(s)	755
BaeI	ACNNNNGTAYC	2 cut(s)	834, 867
BanI	GGYRCC	1 cut(s)	227
BanII	GRGCYC	1 cut(s)	799
Bbv12I	GWGCWC	3 cut(s)	676, 739, 799
BbvI	GCAGC	2 cut(s)	715, 1074
BccI	CCATC	6 cut(s)	10, 380, 403, 539, 700, 775
BceAI	ACGGC	2 cut(s)	695, 741
BciT130I	CCWGG	5 cut(s)	297, 369, 741, 753, 1137
BciVI	GTATCC	1 cut(s)	267
BcoDI	GTCTC	1 cut(s)	882
BfaI	CTAG	4 cut(s)	66, 540, 975, 1228
BfuAI	ACCTGC	1 cut(s)	70
BfuI	GTATCC	1 cut(s)	267
BisI	GCNGC	5 cut(s)	268, 729, 810, 1088, 1222
BlsI	GCNGC	5 cut(s)	269, 730, 811, 1089, 1223
Bme1390I	CCNGG	5 cut(s)	297, 369, 741, 753, 1137
Bme18I	GGWCC	1 cut(s)	755
BmeT110I	CYCGRG	1 cut(s)	879
BmgT120I	GGNCC	2 cut(s)	755, 1009
BmiI	GGNNCC	3 cut(s)	229, 499, 837
BmrFI	CCNGG	5 cut(s)	297, 369, 741, 753, 1137
BmsI	GCATC	2 cut(s)	556, 641
BplI	GAGNNNNNCTC	2 cut(s)	1091, 1123
BpmI	CTGGAG	1 cut(s)	831
BpuEI	CTTGAG	2 cut(s)	154, 162
BsaI	GGTCTC	1 cut(s)	882
BsaJI	CCNNGG	1 cut(s)	262
BsaWI	WCCGGW	1 cut(s)	1065
Bsc4I	CCNNNNNNNGG	3 cut(s)	155, 224, 266
Bse1I	ACTGG	2 cut(s)	93, 848
Bse3DI	GCAATG	3 cut(s)	258, 828, 1189
BseBI	CCWGG	5 cut(s)	297, 369, 741, 753, 1137
BseDI	CCNNGG	1 cut(s)	262
BseGI	GGATG	1 cut(s)	786
BseLI	CCNNNNNNNGG	3 cut(s)	155, 224, 266
BseMI	GCAATG	3 cut(s)	258, 828, 1189
BseMII	CTCAG	6 cut(s)	105, 390, 522, 590, 650, 1110
BseNI	ACTGG	2 cut(s)	93, 848
BseXI	GCAGC	2 cut(s)	715, 1074
BseYI	CCCAGC	2 cut(s)	872, 1011
BsgI	GTGCAG	1 cut(s)	636
BshFI	GGCC	4 cut(s)	167, 710, 1010, 1224
BshNI	GGYRCC	1 cut(s)	227
BsiHKAI	GWGCWC	3 cut(s)	676, 739, 799
BsiHKCI	CYCGRG	1 cut(s)	879
BsiSI	CCGG	1 cut(s)	1066
BslI	CCNNNNNNNGG	3 cut(s)	155, 224, 266
BsmAI	GTCTC	1 cut(s)	882
BsmI	GAATGC	1 cut(s)	1001
BsnI	GGCC	4 cut(s)	167, 710, 1010, 1224
Bso31I	GGTCTC	1 cut(s)	882
BsoBI	CYCGRG	1 cut(s)	879
Bsp1286I	GDGCHC	3 cut(s)	676, 739, 799
Bsp143I	GATC	5 cut(s)	299, 428, 971, 1052, 1068
Bsp19I	CCATGG	1 cut(s)	262
BspACI	CCGC	5 cut(s)	267, 810, 963, 992, 1221
BspANI	GGCC	4 cut(s)	167, 710, 1010, 1224
BspCNI	CTCAG	6 cut(s)	106, 391, 523, 589, 649, 1109
BspLI	GGNNCC	3 cut(s)	229, 499, 837
BspMI	ACCTGC	1 cut(s)	70
BspPI	GGATC	2 cut(s)	307, 1076
BspT107I	GGYRCC	1 cut(s)	227
BspTNI	GGTCTC	1 cut(s)	882
BsrBI	CCGCTC	1 cut(s)	965
BsrDI	GCAATG	3 cut(s)	258, 828, 1189
BsrI	ACTGG	2 cut(s)	93, 848
BssECI	CCNNGG	1 cut(s)	262
BssMI	GATC	5 cut(s)	299, 428, 971, 1052, 1068
BssT1I	CCWWGG	1 cut(s)	262
Bst2UI	CCWGG	5 cut(s)	297, 369, 741, 753, 1137
Bst4CI	ACNGT	3 cut(s)	106, 691, 1155
BstAPI	GCANNNNNTGC	1 cut(s)	214
BstC8I	GCNNGC	6 cut(s)	100, 819, 986, 990, 1008, 1017
BstDEI	CTNAG	7 cut(s)	114, 399, 531, 576, 636, 1049, 1096
BstDSI	CCRYGG	1 cut(s)	262
BstF5I	GGATG	1 cut(s)	786
BstHHI	GCGC	1 cut(s)	984
BstKTI	GATC	5 cut(s)	302, 431, 974, 1055, 1071
BstMAI	GTCTC	1 cut(s)	882
BstMBI	GATC	5 cut(s)	299, 428, 971, 1052, 1068
BstMWI	GCNNNNNNNGC	7 cut(s)	214, 536, 734, 818, 823, 1012, 1016
BstNI	CCWGG	5 cut(s)	297, 369, 741, 753, 1137
BstSCI	CCNGG	5 cut(s)	295, 367, 739, 751, 1135
BstV1I	GCAGC	2 cut(s)	715, 1074
BstX2I	RGATCY	1 cut(s)	299
BstYI	RGATCY	1 cut(s)	299
BsuI	GTATCC	1 cut(s)	267
BsuRI	GGCC	4 cut(s)	167, 710, 1010, 1224
BtgI	CCRYGG	1 cut(s)	262
BtgZI	GCGATG	1 cut(s)	1014
BtsCI	GGATG	1 cut(s)	786
BtsI	GCAGTG	1 cut(s)	16
BtsIMutI	CAGTG	3 cut(s)	16, 100, 102
BveI	ACCTGC	1 cut(s)	70
Cac8I	GCNNGC	6 cut(s)	100, 819, 986, 990, 1008, 1017
CfoI	GCGC	1 cut(s)	984
Cfr13I	GGNCC	2 cut(s)	755, 1009
Csp6I	GTAC	4 cut(s)	69, 228, 549, 1151
CviAII	CATG	7 cut(s)	155, 263, 376, 656, 715, 814, 1217
CviQI	GTAC	4 cut(s)	69, 228, 549, 1151
DdeI	CTNAG	7 cut(s)	114, 399, 531, 576, 636, 1049, 1096
DpnI	GATC	5 cut(s)	301, 430, 973, 1054, 1070
DpnII	GATC	5 cut(s)	299, 428, 971, 1052, 1068
EaeI	YGGCCR	1 cut(s)	708
EciI	GGCGGA	1 cut(s)	1007
Ecl136II	GAGCTC	1 cut(s)	797
Eco130I	CCWWGG	1 cut(s)	262
Eco24I	GRGCYC	1 cut(s)	799
Eco31I	GGTCTC	1 cut(s)	882
Eco47I	GGWCC	1 cut(s)	755
Eco53kI	GAGCTC	1 cut(s)	797
Eco57I	CTGAAG	1 cut(s)	1041
Eco88I	CYCGRG	1 cut(s)	879
EcoICRI	GAGCTC	1 cut(s)	797
EcoRII	CCWGG	5 cut(s)	295, 367, 739, 751, 1135
EcoT14I	CCWWGG	1 cut(s)	262
EcoT22I	ATGCAT	1 cut(s)	634
EcoT38I	GRGCYC	1 cut(s)	799
ErhI	CCWWGG	1 cut(s)	262
FaeI	CATG	7 cut(s)	158, 266, 379, 659, 718, 817, 1220
FatI	CATG	7 cut(s)	154, 262, 375, 655, 714, 813, 1216
FauNDI	CATATG	1 cut(s)	244
Fnu4HI	GCNGC	5 cut(s)	268, 729, 810, 1088, 1222
FokI	GGATG	1 cut(s)	793
FriOI	GRGCYC	1 cut(s)	799
Fsp4HI	GCNGC	5 cut(s)	268, 729, 810, 1088, 1222
FspBI	CTAG	4 cut(s)	66, 540, 975, 1228
FspI	TGCGCA	1 cut(s)	983
GlaI	GCGC	1 cut(s)	983
GluI	GCNGC	5 cut(s)	268, 729, 810, 1088, 1222
GsaI	CCCAGC	2 cut(s)	876, 1015
GsuI	CTGGAG	1 cut(s)	831
HaeIII	GGCC	4 cut(s)	167, 710, 1010, 1224
HapII	CCGG	1 cut(s)	1066
HhaI	GCGC	1 cut(s)	984
Hin1II	CATG	7 cut(s)	158, 266, 379, 659, 718, 817, 1220
Hin6I	GCGC	1 cut(s)	982
HinP1I	GCGC	1 cut(s)	982
HincII	GTYRAC	1 cut(s)	1063
HindII	GTYRAC	1 cut(s)	1063
HindIII	AAGCTT	1 cut(s)	439
HinfI	GANTC	4 cut(s)	584, 614, 697, 959
HpaI	GTTAAC	1 cut(s)	1063
HpaII	CCGG	1 cut(s)	1066
HphI	GGTGA	2 cut(s)	176, 272
Hpy166II	GTNNAC	2 cut(s)	1063, 1169
Hpy188I	TCNGA	6 cut(s)	400, 579, 639, 702, 1057, 1099
Hpy188III	TCNNGA	6 cut(s)	113, 123, 495, 881, 1072, 1142
Hpy8I	GTNNAC	2 cut(s)	1063, 1169
Hpy99I	CGWCG	1 cut(s)	972
HpyAV	CCTTC	5 cut(s)	37, 551, 617, 813, 826
HpyCH4III	ACNGT	3 cut(s)	106, 691, 1155
HpyCH4V	TGCA	7 cut(s)	251, 569, 632, 653, 761, 821, 1202
HpyF10VI	GCNNNNNNNGC	7 cut(s)	214, 536, 734, 818, 823, 1012, 1016
HpyF3I	CTNAG	7 cut(s)	114, 399, 531, 576, 636, 1049, 1096
Hsp92II	CATG	7 cut(s)	158, 266, 379, 659, 718, 817, 1220
HspAI	GCGC	1 cut(s)	982
KpnI	GGTACC	1 cut(s)	231
KspAI	GTTAAC	1 cut(s)	1063
Kzo9I	GATC	5 cut(s)	299, 428, 971, 1052, 1068
LmnI	GCTCC	7 cut(s)	76, 497, 671, 742, 794, 802, 1074
Lsp1109I	GCAGC	2 cut(s)	715, 1074
LweI	GCATC	2 cut(s)	556, 641
MaeI	CTAG	4 cut(s)	66, 540, 975, 1228
MaeIII	GTNAC	6 cut(s)	359, 581, 865, 894, 906, 1139
MalI	GATC	5 cut(s)	301, 430, 973, 1054, 1070
MbiI	CCGCTC	1 cut(s)	965
MboI	GATC	5 cut(s)	299, 428, 971, 1052, 1068
MboII	GAAGA	2 cut(s)	481, 1157
MfeI	CAATTG	1 cut(s)	590
MflI	RGATCY	1 cut(s)	299
MhlI	GDGCHC	3 cut(s)	676, 739, 799
MluCI	AATT	5 cut(s)	413, 513, 571, 590, 1131
MlyI	GAGTC	1 cut(s)	578
MmeI	TCCRAC	1 cut(s)	863
Mph1103I	ATGCAT	1 cut(s)	634
MroXI	GAANNNNTTC	1 cut(s)	517
MseI	TTAA	5 cut(s)	108, 770, 927, 1028, 1062
MslI	CAYNNNNRTG	2 cut(s)	243, 1146
MspI	CCGG	1 cut(s)	1066
MspR9I	CCNGG	5 cut(s)	297, 369, 741, 753, 1137
MunI	CAATTG	1 cut(s)	590
Mva1269I	GAATGC	1 cut(s)	1001
MvaI	CCWGG	5 cut(s)	297, 369, 741, 753, 1137
MwoI	GCNNNNNNNGC	7 cut(s)	214, 536, 734, 818, 823, 1012, 1016
NcoI	CCATGG	1 cut(s)	262
NdeI	CATATG	1 cut(s)	244
NdeII	GATC	5 cut(s)	299, 428, 971, 1052, 1068
NlaIII	CATG	7 cut(s)	158, 266, 379, 659, 718, 817, 1220
NlaIV	GGNNCC	3 cut(s)	229, 499, 837
NmuCI	GTSAC	3 cut(s)	581, 894, 1139
NsbI	TGCGCA	1 cut(s)	983
NsiI	ATGCAT	1 cut(s)	634
PaqCI	CACCTGC	1 cut(s)	70
PctI	GAATGC	1 cut(s)	1001
PdmI	GAANNNNTTC	1 cut(s)	517
PfeI	GAWTC	3 cut(s)	614, 697, 959
PflMI	CCANNNNNTGG	1 cut(s)	224
PfoI	TCCNGGA	1 cut(s)	295
PkrI	GCNGC	5 cut(s)	269, 730, 811, 1089, 1223
PleI	GAGTC	1 cut(s)	578
PpsI	GAGTC	1 cut(s)	578
Psp124BI	GAGCTC	1 cut(s)	799
Psp6I	CCWGG	5 cut(s)	295, 367, 739, 751, 1135
PspFI	CCCAGC	2 cut(s)	872, 1011
PspGI	CCWGG	5 cut(s)	295, 367, 739, 751, 1135
PspN4I	GGNNCC	3 cut(s)	229, 499, 837
PspPI	GGNCC	2 cut(s)	755, 1009
PsuI	RGATCY	1 cut(s)	299
RsaI	GTAC	4 cut(s)	70, 229, 550, 1152
RsaNI	GTAC	4 cut(s)	69, 228, 549, 1151
RseI	CAYNNNNRTG	2 cut(s)	243, 1146
SacI	GAGCTC	1 cut(s)	799
SaqAI	TTAA	5 cut(s)	108, 770, 927, 1028, 1062
SatI	GCNGC	5 cut(s)	268, 729, 810, 1088, 1222
Sau3AI	GATC	5 cut(s)	299, 428, 971, 1052, 1068
Sau96I	GGNCC	2 cut(s)	755, 1009
SchI	GAGTC	1 cut(s)	578
ScrFI	CCNGG	5 cut(s)	297, 369, 741, 753, 1137
SduI	GDGCHC	3 cut(s)	676, 739, 799
SfaNI	GCATC	2 cut(s)	556, 641
SinI	GGWCC	1 cut(s)	755
SmiMI	CAYNNNNRTG	2 cut(s)	243, 1146
SmlI	CTYRAG	2 cut(s)	169, 177
SmoI	CTYRAG	2 cut(s)	169, 177
Sse9I	AATT	5 cut(s)	413, 513, 571, 590, 1131
SsiI	CCGC	5 cut(s)	267, 810, 963, 992, 1221
SspI	AATATT	1 cut(s)	775
SspMI	CTAG	4 cut(s)	66, 540, 975, 1228
SstI	GAGCTC	1 cut(s)	799
StyD4I	CCNGG	5 cut(s)	295, 367, 739, 751, 1135
StyI	CCWWGG	1 cut(s)	262
TaaI	ACNGT	3 cut(s)	106, 691, 1155
TaqI	TCGA	1 cut(s)	970
TasI	AATT	5 cut(s)	413, 513, 571, 590, 1131
TauI	GCSGC	3 cut(s)	270, 812, 1224
TfiI	GAWTC	3 cut(s)	614, 697, 959
Tru1I	TTAA	5 cut(s)	108, 770, 927, 1028, 1062
Tru9I	TTAA	5 cut(s)	108, 770, 927, 1028, 1062
TscAI	CASTG	3 cut(s)	16, 100, 109
TseFI	GTSAC	3 cut(s)	581, 894, 1139
TseI	GCWGC	2 cut(s)	728, 1087
Tsp45I	GTSAC	3 cut(s)	581, 894, 1139
TspRI	CASTG	3 cut(s)	16, 100, 109
Van91I	CCANNNNNTGG	1 cut(s)	224
VpaK11BI	GGWCC	1 cut(s)	755
XapI	RAATTY	3 cut(s)	513, 571, 1131
XmnI	GAANNNNTTC	1 cut(s)	517
XspI	CTAG	4 cut(s)	66, 540, 975, 1228
Zsp2I	ATGCAT	1 cut(s)	634

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.