Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004692

Physical Location & Seq

Genomic Coordinates Forward (+)

8363471 .. 8369751

Gene Structure 6281 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1098500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGGAGCTTCGTTCTCGCAAGCGCACCGCCGGCCCTTCGTCCGATGGAAATGAGCAACACGAGGAAAGGGAAGATGCTTATTCACCGTCTGATGAGGATACTTATGTACCACCGTCTTCGCCTTCCGCTTCTGATTTTGAGAGTGAAGAAGGTGAAGAGTACCAAAACAATGTGGAAGTTGTTTTTGACTTGAATGATAAACCTAATGGTAAAATGAAGGAGCCTGCGATAAAAAAGTCGAAGGTGGTGGTGGAGAACAAGGCAGAAGCAGGAGGACCAAGCTCGATGGTGCCAGCCCCCAGGTTCACATCAGGTGAAATTGACGATCTTAGGTTGCACGACAAAAAAAAGAAAAAGAATCGTAAATCTGGGGGGCAAAAGCGGCGTTGGCAGTGGAATACGTGGGAAGAGGAGCATGATAAATGGCTTGATGACAACGTAACTGAAGATTTTGATCTGGATACTCAGAAGAATGTGATATGCGATGCTGCTGAGGCACCATCTGATCTGATTATGCCTTTGCTAAGGTACCAGAAGGAATGGTTGTCATGGGCCGTGAAACAGGAAGAGTCTGAAACTAGAGGAGGAATCCTAGCAGATGAGATGGGAATGGGGAAGACTATTCAAGCCATTACTCTTGTCCTTGCTAAACGGGAACTTAATTCGACATGTTATGACACTGGTTCTTCCACAAGCTTTCCTAGAATCAAAACCACCCTTGTAGTTTGCCCTGTAGTTGCTGTGAGTCAATGGGTTAGTGAAATAGAGCGGTTTACTTCAAAGGGAAGTACAAAGGTTTTGGTTTATCATGGGGCTAATAGAGAGAAGAGTTCCCAAATTTTCTCAGAGTATGATTTTGTCATTACTACATACTCTATTGTCGAGGCTGACTACAGGAAGAATGTGATGCCTCCCAAACAAAAGTGTCAATATTGTGGAAAGTTGTATTACGAGAAGAAAATGTCAACTCACTTAAAATATTTTTGTGGGCCTAACGCTTTTAGAACTGAGAAGCAGTCTAAGCAACAAAGGAGAAAGCATCCACAACCAGTGTCGCAGAAGACATTTGAGTCTAAAGGAAAGAATGGAGCTTCCAGGAAGAGATCAGCACTGAACGAGGAAGATGATATGGATTCAGAAGATATTGGACAGCGCTTGAGCCTAGATAAGTCAGTTTTGCATTCTGTGAAGTGGAACCGTATCATTTTGGATGAGGCCCATTATATAAAATCAAGGCGTTGTAATACTGCGAAAGCAGTTCTTGCTTTAGAGTCTTCATACAAATGGGCTCTAAGTGGCACACCTCTTCAGAACCGTGTTGGAGAACTTTATTCTTTGATCCGCTTCTTGCAGTTAGTCCCCTACTCATATTACTTATGCAAGGATTGTGATTGCAGGACTCTTGATCATAGCTCCGCAGCACAATGTTCGAATTGCCCTCATAATTCAGTTAGACATTTTTGTTGGTGGAATAAATATGTGGCGACACCGATACAGATATATGGAAATCATATCCAAGGCAAAAGGGCCATGTATTTGCTAAAACACAAAATTCTAAAGAACATAGTCCTGCGACGAACTAAAAAGGGCAGGGCTGCTGATCTTGCACTACCTCCTAGAATTGTTTCATTGAGGCGGGACACCTTGGATATCAAAGAACAAGGTTTTTATGAGTCAATATATAATGATACTCAGTCACAATTTAACGCATTTGTCGAAGAGGGAACTGTGATGAACAATTATGCTCACATATTTGACCTTCTCACTCGCTTGCGACAGGCTGTTAATCATCCTTACCTTGTGGTGTATTCTGCATCTGCTGCACTGAGAAATGGAAGCAACATTAATGTCAATGAGAAAATCTGTGGAATTTGTCATGAAGCAGCAGAAGATCCAGTGGTTACTGCTTGTGAGCATGTGTTTTGCAAGGCTTGCTTGCTTGATTTTGCTGCTTCTTTGGGACAAGTCTCATGCCCGACATGCTCTAAACCAGTTACTGTTGATTTCACTACAACACCAGGTCCTGCTAATCAGACCAAGACTACCATTAAGGGGTTTAGATCTTCAAGCATTTTGAACAGAATTAAGCTTGAAAATTTTCAGACTAGCACAAAAATTGAGGCTTTGAGGGAAGAAATCAGATCCATGGTTGAAAAGGATGGCTCTGCAAAAGGAATTGTTTTCAGCCAGTTCACATCGTTCTTGGATATCATTAACTACTCCTTGCAAAAGTCCGGTGTCAATTGTGTTCAGTTGGTCGGAAGCATGACTATGTCTGCAAGGGATAATGCTATAAAGAGATTTACCGAGGATCCAGATTGCAGGATTTTCTTAATGAGCTTGAAAGCTGGAGGTGTTGCTCTTAATCTGACAGTTGCATCGCATGTTTTCTTGATGGACCCGTGGTGGAACCCAGCAGTTGAGAGGCAAGCACAAGATAGAATCCACCGGATAGGGCAGTACAAACCGATCAGAATTATGAGATTTGTGATTGAGAACACTATTGAGGACCGGATTCTAAAGCTACAGGAGAAAAAGGAATTAGTGTTCGAGGGGACCATCGGTGGTGCTTCAGACGCTCTTGCAAAATTAACAGAAGCCGACTTGAGATTCCTCTTTGTCACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000109 GO:0000166 GO:0000720 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004842 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006290 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009987 GO:0016043 GO:0016462 GO:0016567 GO:0016740 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019538 GO:0019787 GO:0030554 GO:0032392 GO:0032446 GO:0032508 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033554 GO:0034641 GO:0035639 GO:0036094 GO:0036211 GO:0042623 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046872 GO:0046914 GO:0050896 GO:0051276 GO:0051716 GO:0061630 GO:0061659 GO:0070035 GO:0070647 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097367 GO:0140096 GO:0140097 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1990391

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Helicase_C SNF2_N zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

876

Amino Acids

99.34

Weight (kDa)

8.65

Isoelectric Point (pI)

50.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNF2-rel_dom	PF00176	177 - 456	4e-49	SNF2-related domain
zf-C3HC4_3	PF13920	619 - 666	1.3e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	622 - 659	6.2e-08	RING-type zinc-finger
zf-C3HC4_2	PF13923	622 - 661	7.6e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	622 - 661	8.3e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	622 - 663	6.7e-06	zinc-RING finger domain
Helicase_C	PF00271	705 - 819	7.2e-20	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004692)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02670 AT1G02670 AT1G02670 AT1G02670 AT1G02670 AT1G02670 AT1G02670 AT1G05120 AT1G05120
fragaria_vesca	FvH4_6g42970 FvH4_6g42970
malus_domestica	MD17G1098500.v1.1
prunus_persica	Prupe.3G215900_v2.0.a1 Prupe.3G215900_v2.0.a1
pyrus_communis	pycom09g03310 pycom17g09390
rosa_chinensis	RchiOBHm_Chr2g0158951
rosa_laevigata	RLG00000021126
rosa_multiflora	Rmu_sc0002845.1_g000016 Rmu_sc0007967.1_g000006 Rmu_sc0013139.1_g000001 Rmu_sc0030100.1_g000001
rosa_roxburghii	Rroxscaffold_1G00030500 Rroxscaffold_2G00090850
rosa_rugosa	Rorug02G0473200
rosa_samantha	Rh2AG539700 Rh2BG552600 Rh2CG523000 Rh2DG562300
rosa_wichuraiana	Rw2G044670 Rw7G039330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1069
Acc65I	GGTACC	1 cut(s)	528
AccB1I	GGYRCC	3 cut(s)	289, 496, 528
AccBSI	CCGCTC	1 cut(s)	769
AciI	CCGC	7 cut(s)	27, 126, 382, 769, 1342, 1416, 1636
AclWI	GGATC	5 cut(s)	1333, 1886, 2136, 2306, 2319
AcsI	RAATTY	4 cut(s)	837, 1551, 1869, 2095
AcuI	CTGAAG	3 cut(s)	465, 1292, 2556
AfaI	GTAC	5 cut(s)	108, 161, 530, 790, 2462
AfeI	AGCGCT	1 cut(s)	1154
AfiI	CCNNNNNNNGG	1 cut(s)	2052
AflIII	ACRYGT	1 cut(s)	668
AgsI	TTSAA	8 cut(s)	193, 626, 780, 2067, 2077, 2093, 2153, 2344
AjnI	CCWGG	3 cut(s)	299, 1094, 2017
AloI	GAACNNNNNNTCC	2 cut(s)	2531, 2563
AluBI	AGCT	9 cut(s)	7, 282, 696, 1091, 1413, 2089, 2340, 2348, 2524
AluI	AGCT	9 cut(s)	7, 282, 696, 1091, 1413, 2089, 2340, 2348, 2524
Alw26I	GTCTC	1 cut(s)	1972
AlwI	GGATC	5 cut(s)	1333, 1886, 2136, 2306, 2319
AlwNI	CAGNNNCTG	2 cut(s)	1997, 2024
Aor51HI	AGCGCT	1 cut(s)	1154
AoxI	GGCC	5 cut(s)	31, 552, 989, 1215, 1527
ApeKI	GCWGC	6 cut(s)	488, 1418, 1595, 1820, 1883, 1949
ApoI	RAATTY	4 cut(s)	837, 1551, 1869, 2095
AseI	ATTAAT	1 cut(s)	1845
Asp700I	GAANNNNTTC	2 cut(s)	1624, 2097
Asp718I	GGTACC	1 cut(s)	528
AspLEI	GCGC	2 cut(s)	24, 1155
AsuHPI	GGTGA	4 cut(s)	75, 164, 326, 2614
AsuII	TTCGAA	1 cut(s)	1430
AvaII	GGWCC	5 cut(s)	275, 2021, 2398, 2509, 2556
BaeI	ACNNNNGTAYC	4 cut(s)	90, 90, 123, 123
BamHI	GGATCC	1 cut(s)	2311
BanI	GGYRCC	3 cut(s)	289, 496, 528
BanII	GRGCYC	1 cut(s)	1291
BauI	CACGAG	1 cut(s)	59
BbsI	GAAGAC	4 cut(s)	108, 623, 1067, 1266
BbvCI	CCTCAGC	1 cut(s)	492
BbvI	GCAGC	6 cut(s)	475, 1430, 1582, 1807, 1895, 1936
BccI	CCATC	7 cut(s)	38, 280, 508, 598, 2153, 2389, 2567
BceAI	ACGGC	1 cut(s)	539
BcgI	CGANNNNNNTGC	2 cut(s)	1410, 1444
BciT130I	CCWGG	3 cut(s)	301, 1096, 2019
BciVI	GTATCC	2 cut(s)	91, 454
BclI	TGATCA	1 cut(s)	1405
BcoDI	GTCTC	1 cut(s)	1972
BfaI	CTAG	6 cut(s)	579, 593, 702, 1163, 1617, 2106
BfmI	CTRYAG	3 cut(s)	732, 892, 2525
BfoI	RGCGCY	1 cut(s)	1156
BfuI	GTATCC	2 cut(s)	91, 454
BglII	AGATCT	1 cut(s)	2060
BisI	GCNGC	7 cut(s)	383, 489, 1419, 1596, 1821, 1884, 1950
BlsI	GCNGC	7 cut(s)	384, 490, 1420, 1597, 1822, 1885, 1951
Bme1390I	CCNGG	3 cut(s)	301, 1096, 2019
Bme18I	GGWCC	5 cut(s)	275, 2021, 2398, 2509, 2556
BmiI	GGNNCC	9 cut(s)	222, 291, 498, 530, 1196, 2313, 2400, 2411, 2557
BmrFI	CCNGG	3 cut(s)	301, 1096, 2019
BmsI	GCATC	6 cut(s)	64, 475, 897, 1048, 1823, 2387
BpiI	GAAGAC	4 cut(s)	108, 623, 1067, 1266
BpmI	CTGGAG	1 cut(s)	2370
Bpu10I	CCTNAGC	2 cut(s)	492, 524
Bpu14I	TTCGAA	1 cut(s)	1430
BpuEI	CTTGAG	2 cut(s)	1177, 2626
BsaAI	YACGTR	1 cut(s)	402
BsaBI	GATNNNNATC	1 cut(s)	453
BsaJI	CCNNGG	6 cut(s)	299, 1516, 1644, 2145, 2307, 2402
BsaWI	WCCGGW	3 cut(s)	2234, 2448, 2511
Bsc4I	CCNNNNNNNGG	1 cut(s)	2052
Bse118I	RCCGGY	1 cut(s)	29
Bse1I	ACTGG	5 cut(s)	685, 1049, 1895, 1991, 2188
Bse8I	GATNNNNATC	1 cut(s)	453
BseBI	CCWGG	3 cut(s)	301, 1096, 2019
BseDI	CCNNGG	6 cut(s)	299, 1516, 1644, 2145, 2307, 2402
BseGI	GGATG	4 cut(s)	1039, 1216, 1789, 2164
BseJI	GATNNNNATC	1 cut(s)	453
BseLI	CCNNNNNNNGG	1 cut(s)	2052
BseMII	CTCAG	6 cut(s)	479, 483, 858, 999, 1706, 1817
BseNI	ACTGG	5 cut(s)	685, 1049, 1895, 1991, 2188
BseRI	GAGGAG	2 cut(s)	425, 597
BseXI	GCAGC	6 cut(s)	475, 1430, 1582, 1807, 1895, 1936
BseYI	CCCAGC	1 cut(s)	2413
BsgI	GTGCAG	1 cut(s)	1806
BshFI	GGCC	5 cut(s)	33, 554, 991, 1217, 1529
BshNI	GGYRCC	3 cut(s)	289, 496, 528
BsiSI	CCGG	4 cut(s)	30, 2235, 2449, 2512
BslFI	GGGAC	4 cut(s)	1343, 1652, 1974, 2569
BslI	CCNNNNNNNGG	1 cut(s)	2052
BsmAI	GTCTC	1 cut(s)	1972
BsmFI	GGGAC	4 cut(s)	1343, 1652, 1974, 2569
BsmI	GAATGC	1 cut(s)	1180
BsnI	GGCC	5 cut(s)	33, 554, 991, 1217, 1529
Bsp119I	TTCGAA	1 cut(s)	1430
Bsp1286I	GDGCHC	1 cut(s)	1291
Bsp19I	CCATGG	1 cut(s)	2145
BspACI	CCGC	7 cut(s)	27, 126, 382, 769, 1342, 1416, 1636
BspANI	GGCC	5 cut(s)	33, 554, 991, 1217, 1529
BspCNI	CTCAG	6 cut(s)	478, 484, 857, 1000, 1705, 1818
BspHI	TCATGA	1 cut(s)	1876
BspLI	GGNNCC	9 cut(s)	222, 291, 498, 530, 1196, 2313, 2400, 2411, 2557
BspPI	GGATC	5 cut(s)	1333, 1886, 2136, 2306, 2319
BspT104I	TTCGAA	1 cut(s)	1430
BspT107I	GGYRCC	3 cut(s)	289, 496, 528
BsrBI	CCGCTC	1 cut(s)	769
BsrFI	RCCGGY	1 cut(s)	29
BsrI	ACTGG	5 cut(s)	685, 1049, 1895, 1991, 2188
BssAI	RCCGGY	1 cut(s)	29
BssECI	CCNNGG	6 cut(s)	299, 1516, 1644, 2145, 2307, 2402
BssSI	CACGAG	1 cut(s)	59
BssT1I	CCWWGG	3 cut(s)	1516, 1644, 2145
Bst2BI	CACGAG	1 cut(s)	59
Bst2UI	CCWGG	3 cut(s)	301, 1096, 2019
Bst4CI	ACNGT	7 cut(s)	87, 114, 1199, 1316, 1729, 1999, 2374
Bst6I	CTCTTC	7 cut(s)	150, 402, 561, 821, 1094, 1311, 1713
BstAPI	GCANNNNNTGC	3 cut(s)	1262, 1820, 1932
BstBAI	YACGTR	1 cut(s)	402
BstBI	TTCGAA	1 cut(s)	1430
BstC8I	GCNNGC	8 cut(s)	20, 31, 225, 294, 1772, 1933, 1937, 2430
BstDSI	CCRYGG	2 cut(s)	2145, 2402
BstF5I	GGATG	4 cut(s)	1039, 1216, 1789, 2164
BstH2I	RGCGCY	1 cut(s)	1156
BstHHI	GCGC	2 cut(s)	24, 1155
BstMAI	GTCTC	1 cut(s)	1972
BstNI	CCWGG	3 cut(s)	301, 1096, 2019
BstNSI	RCATGY	4 cut(s)	672, 1919, 1983, 2387
BstSCI	CCNGG	3 cut(s)	299, 1094, 2017
BstSFI	CTRYAG	3 cut(s)	732, 892, 2525
BstV1I	GCAGC	6 cut(s)	475, 1430, 1582, 1807, 1895, 1936
BstV2I	GAAGAC	4 cut(s)	108, 623, 1067, 1266
BstX2I	RGATCY	4 cut(s)	1891, 2060, 2141, 2311
BstYI	RGATCY	4 cut(s)	1891, 2060, 2141, 2311
BsuI	GTATCC	2 cut(s)	91, 454
BsuRI	GGCC	5 cut(s)	33, 554, 991, 1217, 1529
BtgI	CCRYGG	2 cut(s)	2145, 2402
BtgZI	GCGATG	2 cut(s)	498, 2364
BtsCI	GGATG	4 cut(s)	1039, 1216, 1789, 2164
BtsI	GCAGTG	1 cut(s)	398
BtsIMutI	CAGTG	6 cut(s)	398, 678, 1056, 1109, 1823, 1902
Cac8I	GCNNGC	8 cut(s)	20, 31, 225, 294, 1772, 1933, 1937, 2430
CaiI	CAGNNNCTG	2 cut(s)	1997, 2024
CciI	TCATGA	1 cut(s)	1876
CfoI	GCGC	2 cut(s)	24, 1155
Cfr10I	RCCGGY	1 cut(s)	29
CseI	GACGC	1 cut(s)	2585
CsiI	ACCWGGT	1 cut(s)	2017
Csp6I	GTAC	5 cut(s)	107, 160, 529, 789, 2461
CviQI	GTAC	5 cut(s)	107, 160, 529, 789, 2461
DrdI	GACNNNNNNGTC	1 cut(s)	1069
DseDI	GACNNNNNNGTC	1 cut(s)	1069
Eam1104I	CTCTTC	7 cut(s)	150, 402, 561, 821, 1094, 1311, 1713
EarI	CTCTTC	7 cut(s)	150, 402, 561, 821, 1094, 1311, 1713
Eco130I	CCWWGG	3 cut(s)	1516, 1644, 2145
Eco24I	GRGCYC	1 cut(s)	1291
Eco32I	GATATC	2 cut(s)	1651, 2209
Eco47I	GGWCC	5 cut(s)	275, 2021, 2398, 2509, 2556
Eco47III	AGCGCT	1 cut(s)	1154
Eco57I	CTGAAG	3 cut(s)	465, 1292, 2556
EcoO109I	RGGNCCY	1 cut(s)	2021
EcoRII	CCWGG	3 cut(s)	299, 1094, 2017
EcoRV	GATATC	2 cut(s)	1651, 2209
EcoT14I	CCWWGG	3 cut(s)	1516, 1644, 2145
EcoT38I	GRGCYC	1 cut(s)	1291
ErhI	CCWWGG	3 cut(s)	1516, 1644, 2145
FalI	AAGNNNNNCTT	4 cut(s)	1245, 1277, 1919, 1951
FaqI	GGGAC	4 cut(s)	1343, 1652, 1974, 2569
FauI	CCCGC	1 cut(s)	1629
FbaI	TGATCA	1 cut(s)	1405
Fnu4HI	GCNGC	7 cut(s)	383, 489, 1419, 1596, 1821, 1884, 1950
FokI	GGATG	4 cut(s)	1026, 1223, 1776, 2171
FriOI	GRGCYC	1 cut(s)	1291
Fsp4HI	GCNGC	7 cut(s)	383, 489, 1419, 1596, 1821, 1884, 1950
FspBI	CTAG	6 cut(s)	579, 593, 702, 1163, 1617, 2106
GlaI	GCGC	2 cut(s)	23, 1154
GluI	GCNGC	7 cut(s)	383, 489, 1419, 1596, 1821, 1884, 1950
GsaI	CCCAGC	1 cut(s)	2417
GsuI	CTGGAG	1 cut(s)	2370
HaeII	RGCGCY	1 cut(s)	1156
HaeIII	GGCC	5 cut(s)	33, 554, 991, 1217, 1529
HapII	CCGG	4 cut(s)	30, 2235, 2449, 2512
HgaI	GACGC	1 cut(s)	2585
HhaI	GCGC	2 cut(s)	24, 1155
Hin6I	GCGC	2 cut(s)	22, 1153
HinP1I	GCGC	2 cut(s)	22, 1153
HincII	GTYRAC	1 cut(s)	966
HindII	GTYRAC	1 cut(s)	966
HindIII	AAGCTT	2 cut(s)	694, 2087
HpaII	CCGG	4 cut(s)	30, 2235, 2449, 2512
HphI	GGTGA	4 cut(s)	75, 164, 326, 2614
Hpy166II	GTNNAC	4 cut(s)	306, 774, 966, 2193
Hpy188III	TCNNGA	5 cut(s)	458, 1403, 1877, 2315, 2392
Hpy8I	GTNNAC	4 cut(s)	306, 774, 966, 2193
Hpy99I	CGWCG	1 cut(s)	1578
HpyAV	CCTTC	7 cut(s)	45, 132, 143, 211, 235, 529, 1769
HpyCH4III	ACNGT	7 cut(s)	87, 114, 1199, 1316, 1729, 1999, 2374
HpyCH4IV	ACGT	2 cut(s)	401, 438
HpySE526I	ACGT	2 cut(s)	401, 438
HspAI	GCGC	2 cut(s)	22, 1153
KpnI	GGTACC	1 cut(s)	532
KroI	GCCGGC	1 cut(s)	29
KroNI	GCCGGC	1 cut(s)	31
Ksp22I	TGATCA	1 cut(s)	1405
LmnI	GCTCC	5 cut(s)	4, 220, 412, 1088, 1418
Lsp1109I	GCAGC	6 cut(s)	475, 1430, 1582, 1807, 1895, 1936
LweI	GCATC	6 cut(s)	64, 475, 897, 1048, 1823, 2387
MabI	ACCWGGT	1 cut(s)	2017
MaeI	CTAG	6 cut(s)	579, 593, 702, 1163, 1617, 2106
MaeII	ACGT	2 cut(s)	401, 438
MaeIII	GTNAC	5 cut(s)	439, 1695, 1900, 1993, 2620
MbiI	CCGCTC	1 cut(s)	769
MfeI	CAATTG	1 cut(s)	2242
MflI	RGATCY	4 cut(s)	1891, 2060, 2141, 2311
MhlI	GDGCHC	1 cut(s)	1291
MlyI	GAGTC	6 cut(s)	578, 754, 1079, 1280, 1393, 1682
MmeI	TCCRAC	2 cut(s)	1300, 2239
MroNI	GCCGGC	1 cut(s)	29
MroXI	GAANNNNTTC	2 cut(s)	1624, 2097
MslI	CAYNNNNRTG	2 cut(s)	1282, 2270
MspI	CCGG	4 cut(s)	30, 2235, 2449, 2512
MspR9I	CCNGG	3 cut(s)	301, 1096, 2019
MunI	CAATTG	1 cut(s)	2242
Mva1269I	GAATGC	1 cut(s)	1180
MvaI	CCWGG	3 cut(s)	301, 1096, 2019
NaeI	GCCGGC	1 cut(s)	31
NcoI	CCATGG	1 cut(s)	2145
NgoMIV	GCCGGC	1 cut(s)	29
NlaIV	GGNNCC	9 cut(s)	222, 291, 498, 530, 1196, 2313, 2400, 2411, 2557
NmuCI	GTSAC	2 cut(s)	1695, 2620
NspI	RCATGY	4 cut(s)	672, 1919, 1983, 2387
NspV	TTCGAA	1 cut(s)	1430
PagI	TCATGA	1 cut(s)	1876
PciI	ACATGT	1 cut(s)	668
PcsI	WCGNNNNNNNCGW	1 cut(s)	1713
PctI	GAATGC	1 cut(s)	1180
PdiI	GCCGGC	1 cut(s)	31
PdmI	GAANNNNTTC	2 cut(s)	1624, 2097
PfeI	GAWTC	7 cut(s)	358, 588, 705, 1133, 2442, 2515, 2610
PflFI	GACNNNGTC	1 cut(s)	2272
PfoI	TCCNGGA	1 cut(s)	1094
PkrI	GCNGC	7 cut(s)	384, 490, 1420, 1597, 1822, 1885, 1951
PleI	GAGTC	6 cut(s)	577, 753, 1078, 1279, 1393, 1681
PpsI	GAGTC	6 cut(s)	577, 753, 1078, 1279, 1393, 1681
Ppu21I	YACGTR	1 cut(s)	402
PpuMI	RGGWCCY	1 cut(s)	2021
PscI	ACATGT	1 cut(s)	668
PshBI	ATTAAT	1 cut(s)	1845
Psp5II	RGGWCCY	1 cut(s)	2021
Psp6I	CCWGG	3 cut(s)	299, 1094, 2017
PspFI	CCCAGC	1 cut(s)	2413
PspGI	CCWGG	3 cut(s)	299, 1094, 2017
PspN4I	GGNNCC	9 cut(s)	222, 291, 498, 530, 1196, 2313, 2400, 2411, 2557
PspPPI	RGGWCCY	1 cut(s)	2021
PstNI	CAGNNNCTG	2 cut(s)	1997, 2024
PsuI	RGATCY	4 cut(s)	1891, 2060, 2141, 2311
PsyI	GACNNNGTC	1 cut(s)	2272
RsaI	GTAC	5 cut(s)	108, 161, 530, 790, 2462
RsaNI	GTAC	5 cut(s)	107, 160, 529, 789, 2461
RseI	CAYNNNNRTG	2 cut(s)	1282, 2270
SatI	GCNGC	7 cut(s)	383, 489, 1419, 1596, 1821, 1884, 1950
SchI	GAGTC	6 cut(s)	578, 754, 1079, 1280, 1393, 1682
ScrFI	CCNGG	3 cut(s)	301, 1096, 2019
SduI	GDGCHC	1 cut(s)	1291
SexAI	ACCWGGT	1 cut(s)	2017
SfaNI	GCATC	6 cut(s)	64, 475, 897, 1048, 1823, 2387
SfcI	CTRYAG	3 cut(s)	732, 892, 2525
SfuI	TTCGAA	1 cut(s)	1430
SinI	GGWCC	5 cut(s)	275, 2021, 2398, 2509, 2556
SmiMI	CAYNNNNRTG	2 cut(s)	1282, 2270
SmlI	CTYRAG	2 cut(s)	1156, 2605
SmoI	CTYRAG	2 cut(s)	1156, 2605
SsiI	CCGC	7 cut(s)	27, 126, 382, 769, 1342, 1416, 1636
SspI	AATATT	2 cut(s)	932, 980
SspMI	CTAG	6 cut(s)	579, 593, 702, 1163, 1617, 2106
StyD4I	CCNGG	3 cut(s)	299, 1094, 2017
StyI	CCWWGG	3 cut(s)	1516, 1644, 2145
TaaI	ACNGT	7 cut(s)	87, 114, 1199, 1316, 1729, 1999, 2374
TaiI	ACGT	2 cut(s)	404, 441
TaqI	TCGA	7 cut(s)	239, 284, 665, 882, 1430, 1716, 2550
TatI	WGTACW	2 cut(s)	788, 2460
TauI	GCSGC	1 cut(s)	385
TfiI	GAWTC	7 cut(s)	358, 588, 705, 1133, 2442, 2515, 2610
TscAI	CASTG	6 cut(s)	398, 685, 1056, 1116, 1830, 1902
TseFI	GTSAC	2 cut(s)	1695, 2620
TseI	GCWGC	6 cut(s)	488, 1418, 1595, 1820, 1883, 1949
Tsp45I	GTSAC	2 cut(s)	1695, 2620
TspDTI	ATGAA	5 cut(s)	230, 1266, 1617, 1748, 1893
TspRI	CASTG	6 cut(s)	398, 685, 1056, 1116, 1830, 1902
Tth111I	GACNNNGTC	1 cut(s)	2272
VpaK11BI	GGWCC	5 cut(s)	275, 2021, 2398, 2509, 2556
VspI	ATTAAT	1 cut(s)	1845
XapI	RAATTY	4 cut(s)	837, 1551, 1869, 2095
XceI	RCATGY	4 cut(s)	672, 1919, 1983, 2387
XmnI	GAANNNNTTC	2 cut(s)	1624, 2097
XspI	CTAG	6 cut(s)	579, 593, 702, 1163, 1617, 2106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.