Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2039, 2373 |
| Acc36I | ACCTGC | 1 cut(s) | 2511 |
| Acc65I | GGTACC | 1 cut(s) | 2414 |
| AccB1I | GGYRCC | 2 cut(s) | 2414, 2460 |
| AciI | CCGC | 5 cut(s) | 442, 767, 1296, 2269, 2418 |
| AclWI | GGATC | 8 cut(s) | 219, 457, 764, 777, 1901, 1953, 2123, 2760 |
| AcsI | RAATTY | 3 cut(s) | 254, 640, 1607 |
| AcuI | CTGAAG | 6 cut(s) | 570, 1037, 1415, 1586, 1646, 2435 |
| AdeI | CACNNNGTG | 1 cut(s) | 1043 |
| AfaI | GTAC | 4 cut(s) | 864, 914, 2416, 2537 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1741, 2138, 2548 |
| AflIII | ACRYGT | 1 cut(s) | 2599 |
| AjnI | CCWGG | 4 cut(s) | 319, 2192, 2523, 2546 |
| Alw21I | GWGCWC | 1 cut(s) | 1366 |
| Alw26I | GTCTC | 2 cut(s) | 167, 2100 |
| AlwI | GGATC | 8 cut(s) | 219, 457, 764, 777, 1901, 1953, 2123, 2760 |
| Ama87I | CYCGRG | 2 cut(s) | 1820, 2384 |
| AoxI | GGCC | 5 cut(s) | 317, 387, 531, 2550, 2566 |
| ApoI | RAATTY | 3 cut(s) | 254, 640, 1607 |
| AseI | ATTAAT | 1 cut(s) | 2400 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1659 |
| Asp718I | GGTACC | 1 cut(s) | 2414 |
| AspLEI | GCGC | 1 cut(s) | 2107 |
| AspS9I | GGNCC | 2 cut(s) | 2550, 2767 |
| AsuC2I | CCSGG | 3 cut(s) | 1943, 2657, 2682 |
| AsuHPI | GGTGA | 6 cut(s) | 38, 503, 1133, 1570, 1684, 2068 |
| AvaI | CYCGRG | 2 cut(s) | 1820, 2384 |
| AvaII | GGWCC | 1 cut(s) | 2767 |
| AxyI | CCTNAGG | 1 cut(s) | 1602 |
| BamHI | GGATCC | 1 cut(s) | 769 |
| BanI | GGYRCC | 2 cut(s) | 2414, 2460 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 519, 551 |
| BauI | CACGAG | 2 cut(s) | 215, 717 |
| BbsI | GAAGAC | 2 cut(s) | 117, 1403 |
| Bbv12I | GWGCWC | 1 cut(s) | 1366 |
| BbvCI | CCTCAGC | 1 cut(s) | 2082 |
| BccI | CCATC | 6 cut(s) | 76, 128, 188, 218, 548, 615 |
| BceAI | ACGGC | 2 cut(s) | 783, 2319 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1798, 1832 |
| BciT130I | CCWGG | 4 cut(s) | 321, 2194, 2525, 2548 |
| BciVI | GTATCC | 1 cut(s) | 1609 |
| BclI | TGATCA | 1 cut(s) | 2301 |
| BcnI | CCSGG | 3 cut(s) | 1943, 2657, 2682 |
| BcoDI | GTCTC | 2 cut(s) | 167, 2100 |
| BfaI | CTAG | 6 cut(s) | 281, 515, 732, 1709, 2543, 2606 |
| BfuAI | ACCTGC | 1 cut(s) | 2511 |
| BfuI | GTATCC | 1 cut(s) | 1609 |
| Bme1390I | CCNGG | 7 cut(s) | 321, 1943, 2194, 2525, 2548, 2657, 2682 |
| Bme18I | GGWCC | 1 cut(s) | 2767 |
| BmeT110I | CYCGRG | 2 cut(s) | 1820, 2384 |
| BmgT120I | GGNCC | 2 cut(s) | 2550, 2767 |
| BmiI | GGNNCC | 4 cut(s) | 771, 1294, 2416, 2462 |
| BmrFI | CCNGG | 7 cut(s) | 321, 1943, 2194, 2525, 2548, 2657, 2682 |
| BpiI | GAAGAC | 2 cut(s) | 117, 1403 |
| BpmI | CTGGAG | 4 cut(s) | 342, 381, 1893, 1896 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2082, 2133 |
| BpuEI | CTTGAG | 5 cut(s) | 89, 878, 953, 1268, 1937 |
| BpuMI | CCSGG | 3 cut(s) | 1943, 2657, 2682 |
| BsaAI | YACGTR | 1 cut(s) | 2261 |
| BsaBI | GATNNNNATC | 2 cut(s) | 585, 1905 |
| BsaI | GGTCTC | 1 cut(s) | 2100 |
| BsaJI | CCNNGG | 3 cut(s) | 1144, 1538, 2547 |
| BsaWI | WCCGGW | 1 cut(s) | 2091 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 315, 345, 2519, 2549 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1741, 2138, 2548 |
| Bse118I | RCCGGY | 1 cut(s) | 315 |
| Bse1I | ACTGG | 5 cut(s) | 710, 1886, 2004, 2237, 2464 |
| Bse21I | CCTNAGG | 1 cut(s) | 1602 |
| Bse3DI | GCAATG | 3 cut(s) | 1555, 1869, 2160 |
| Bse8I | GATNNNNATC | 2 cut(s) | 585, 1905 |
| BseBI | CCWGG | 4 cut(s) | 321, 2194, 2525, 2548 |
| BseDI | CCNNGG | 3 cut(s) | 1144, 1538, 2547 |
| BseGI | GGATG | 9 cut(s) | 79, 109, 127, 169, 355, 973, 1474, 1602, 2173 |
| BseJI | GATNNNNATC | 2 cut(s) | 585, 1905 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1741, 2138, 2548 |
| BseMI | GCAATG | 3 cut(s) | 1555, 1869, 2160 |
| BseMII | CTCAG | 7 cut(s) | 276, 972, 1200, 2073, 2124, 2458, 2658 |
| BseNI | ACTGG | 5 cut(s) | 710, 1886, 2004, 2237, 2464 |
| BseRI | GAGGAG | 1 cut(s) | 1479 |
| BseYI | CCCAGC | 1 cut(s) | 595 |
| BsgI | GTGCAG | 4 cut(s) | 642, 1218, 2310, 2767 |
| BshFI | GGCC | 5 cut(s) | 319, 389, 533, 2552, 2568 |
| BshNI | GGYRCC | 2 cut(s) | 2414, 2460 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1366 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1820, 2384 |
| BsiSI | CCGG | 6 cut(s) | 316, 1778, 1943, 2092, 2657, 2681 |
| BslFI | GGGAC | 2 cut(s) | 612, 2285 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1741, 2138, 2548 |
| BsmAI | GTCTC | 2 cut(s) | 167, 2100 |
| BsmFI | GGGAC | 2 cut(s) | 612, 2285 |
| BsmI | GAATGC | 3 cut(s) | 489, 2395, 2486 |
| BsnI | GGCC | 5 cut(s) | 319, 389, 533, 2552, 2568 |
| Bso31I | GGTCTC | 1 cut(s) | 2100 |
| BsoBI | CYCGRG | 2 cut(s) | 1820, 2384 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1366 |
| Bsp1407I | TGTACA | 2 cut(s) | 912, 2535 |
| Bsp19I | CCATGG | 1 cut(s) | 1538 |
| BspACI | CCGC | 5 cut(s) | 442, 767, 1296, 2269, 2418 |
| BspANI | GGCC | 5 cut(s) | 319, 389, 533, 2552, 2568 |
| BspCNI | CTCAG | 7 cut(s) | 277, 973, 1201, 2074, 2125, 2457, 2659 |
| BspHI | TCATGA | 2 cut(s) | 1239, 2647 |
| BspLI | GGNNCC | 4 cut(s) | 771, 1294, 2416, 2462 |
| BspMI | ACCTGC | 1 cut(s) | 2511 |
| BspPI | GGATC | 8 cut(s) | 219, 457, 764, 777, 1901, 1953, 2123, 2760 |
| BspQI | GCTCTTC | 2 cut(s) | 829, 1056 |
| BspT107I | GGYRCC | 2 cut(s) | 2414, 2460 |
| BspTNI | GGTCTC | 1 cut(s) | 2100 |
| BsrDI | GCAATG | 3 cut(s) | 1555, 1869, 2160 |
| BsrFI | RCCGGY | 1 cut(s) | 315 |
| BsrGI | TGTACA | 2 cut(s) | 912, 2535 |
| BsrI | ACTGG | 5 cut(s) | 710, 1886, 2004, 2237, 2464 |
| BssAI | RCCGGY | 1 cut(s) | 315 |
| BssECI | CCNNGG | 3 cut(s) | 1144, 1538, 2547 |
| BssSI | CACGAG | 2 cut(s) | 215, 717 |
| BssT1I | CCWWGG | 2 cut(s) | 1144, 1538 |
| Bst2BI | CACGAG | 2 cut(s) | 215, 717 |
| Bst2UI | CCWGG | 4 cut(s) | 321, 2194, 2525, 2548 |
| Bst4CI | ACNGT | 6 cut(s) | 48, 1043, 1074, 2150, 2288, 2690 |
| Bst6I | CTCTTC | 9 cut(s) | 30, 93, 141, 165, 522, 829, 1056, 1446, 1458 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1205, 1868 |
| BstAUI | TGTACA | 2 cut(s) | 912, 2535 |
| BstBAI | YACGTR | 1 cut(s) | 2261 |
| BstC8I | GCNNGC | 2 cut(s) | 367, 1068 |
| BstDSI | CCRYGG | 1 cut(s) | 1538 |
| BstF5I | GGATG | 9 cut(s) | 79, 109, 127, 169, 355, 973, 1474, 1602, 2173 |
| BstHHI | GCGC | 1 cut(s) | 2107 |
| BstMAI | GTCTC | 2 cut(s) | 167, 2100 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 665, 764, 1063, 1205, 1805, 1868, 1874, 2111 |
| BstNI | CCWGG | 4 cut(s) | 321, 2194, 2525, 2548 |
| BstNSI | RCATGY | 2 cut(s) | 1070, 2603 |
| BstSCI | CCNGG | 7 cut(s) | 319, 1941, 2192, 2523, 2546, 2655, 2680 |
| BstV2I | GAAGAC | 2 cut(s) | 117, 1403 |
| BstX2I | RGATCY | 5 cut(s) | 211, 769, 1906, 1945, 2752 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2564 |
| BstYI | RGATCY | 5 cut(s) | 211, 769, 1906, 1945, 2752 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1602 |
| BsuI | GTATCC | 1 cut(s) | 1609 |
| BsuRI | GGCC | 5 cut(s) | 319, 389, 533, 2552, 2568 |
| BtgI | CCRYGG | 1 cut(s) | 1538 |
| BtsCI | GGATG | 9 cut(s) | 79, 109, 127, 169, 355, 973, 1474, 1602, 2173 |
| BtsI | GCAGTG | 2 cut(s) | 2754, 2779 |
| BtsIMutI | CAGTG | 4 cut(s) | 1048, 1997, 2754, 2779 |
| BveI | ACCTGC | 1 cut(s) | 2511 |
| Cac8I | GCNNGC | 2 cut(s) | 367, 1068 |
| CciI | TCATGA | 2 cut(s) | 1239, 2647 |
| CfoI | GCGC | 1 cut(s) | 2107 |
| Cfr10I | RCCGGY | 1 cut(s) | 315 |
| Cfr13I | GGNCC | 2 cut(s) | 2550, 2767 |
| CseI | GACGC | 2 cut(s) | 985, 2495 |
| Csp6I | GTAC | 4 cut(s) | 863, 913, 2415, 2536 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 904, 939, 2722, 2757 |
| CviQI | GTAC | 4 cut(s) | 863, 913, 2415, 2536 |
| DraIII | CACNNNGTG | 1 cut(s) | 1043 |
| Eam1104I | CTCTTC | 9 cut(s) | 30, 93, 141, 165, 522, 829, 1056, 1446, 1458 |
| EarI | CTCTTC | 9 cut(s) | 30, 93, 141, 165, 522, 829, 1056, 1446, 1458 |
| EciI | GGCGGA | 1 cut(s) | 457 |
| Eco130I | CCWWGG | 2 cut(s) | 1144, 1538 |
| Eco31I | GGTCTC | 1 cut(s) | 2100 |
| Eco47I | GGWCC | 1 cut(s) | 2767 |
| Eco57I | CTGAAG | 6 cut(s) | 570, 1037, 1415, 1586, 1646, 2435 |
| Eco81I | CCTNAGG | 1 cut(s) | 1602 |
| Eco88I | CYCGRG | 2 cut(s) | 1820, 2384 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2550 |
| EcoRII | CCWGG | 4 cut(s) | 319, 2192, 2523, 2546 |
| EcoT14I | CCWWGG | 2 cut(s) | 1144, 1538 |
| EcoT22I | ATGCAT | 1 cut(s) | 586 |
| ErhI | CCWWGG | 2 cut(s) | 1144, 1538 |
| FaqI | GGGAC | 2 cut(s) | 612, 2285 |
| FauI | CCCGC | 1 cut(s) | 2262 |
| FbaI | TGATCA | 1 cut(s) | 2301 |
| FokI | GGATG | 9 cut(s) | 86, 116, 134, 176, 362, 960, 1481, 1609, 2160 |
| FspBI | CTAG | 6 cut(s) | 281, 515, 732, 1709, 2543, 2606 |
| GlaI | GCGC | 1 cut(s) | 2106 |
| GsaI | CCCAGC | 1 cut(s) | 599 |
| GsuI | CTGGAG | 4 cut(s) | 342, 381, 1893, 1896 |
| HaeIII | GGCC | 5 cut(s) | 319, 389, 533, 2552, 2568 |
| HapII | CCGG | 6 cut(s) | 316, 1778, 1943, 2092, 2657, 2681 |
| HgaI | GACGC | 2 cut(s) | 985, 2495 |
| HhaI | GCGC | 1 cut(s) | 2107 |
| Hin6I | GCGC | 1 cut(s) | 2105 |
| HinP1I | GCGC | 1 cut(s) | 2105 |
| HindIII | AAGCTT | 3 cut(s) | 461, 1568, 2367 |
| HpaII | CCGG | 6 cut(s) | 316, 1778, 1943, 2092, 2657, 2681 |
| HphI | GGTGA | 6 cut(s) | 38, 503, 1133, 1570, 1684, 2068 |
| HpyAV | CCTTC | 8 cut(s) | 331, 1118, 1153, 1822, 2047, 2563, 2614, 2705 |
| HpyCH4III | ACNGT | 6 cut(s) | 48, 1043, 1074, 2150, 2288, 2690 |
| HpyCH4IV | ACGT | 1 cut(s) | 2260 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 665, 764, 1063, 1205, 1805, 1868, 1874, 2111 |
| HpySE526I | ACGT | 1 cut(s) | 2260 |
| HspAI | GCGC | 1 cut(s) | 2105 |
| KpnI | GGTACC | 1 cut(s) | 2418 |
| Ksp22I | TGATCA | 1 cut(s) | 2301 |
| LguI | GCTCTTC | 2 cut(s) | 829, 1056 |
| LmnI | GCTCC | 5 cut(s) | 362, 1874, 2102, 2299, 2744 |
| MaeI | CTAG | 6 cut(s) | 281, 515, 732, 1709, 2543, 2606 |
| MaeII | ACGT | 1 cut(s) | 2260 |
| MaeIII | GTNAC | 5 cut(s) | 1771, 2056, 2572, 2684, 2690 |
| MfeI | CAATTG | 1 cut(s) | 651 |
| MflI | RGATCY | 5 cut(s) | 211, 769, 1906, 1945, 2752 |
| MhlI | GDGCHC | 1 cut(s) | 1366 |
| MlyI | GAGTC | 4 cut(s) | 1252, 2053, 2436, 2569 |
| MmeI | TCCRAC | 2 cut(s) | 802, 1001 |
| Mph1103I | ATGCAT | 1 cut(s) | 586 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1659 |
| MslI | CAYNNNNRTG | 5 cut(s) | 685, 1022, 1044, 1543, 2343 |
| MspA1I | CMGCKG | 2 cut(s) | 767, 2585 |
| MspI | CCGG | 6 cut(s) | 316, 1778, 1943, 2092, 2657, 2681 |
| MspR9I | CCNGG | 7 cut(s) | 321, 1943, 2194, 2525, 2548, 2657, 2682 |
| MunI | CAATTG | 1 cut(s) | 651 |
| Mva1269I | GAATGC | 3 cut(s) | 489, 2395, 2486 |
| MvaI | CCWGG | 4 cut(s) | 321, 2194, 2525, 2548 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 665, 764, 1063, 1205, 1805, 1868, 1874, 2111 |
| NciI | CCSGG | 3 cut(s) | 1943, 2657, 2682 |
| NcoI | CCATGG | 1 cut(s) | 1538 |
| NlaIV | GGNNCC | 4 cut(s) | 771, 1294, 2416, 2462 |
| NmuCI | GTSAC | 3 cut(s) | 2056, 2572, 2690 |
| NsiI | ATGCAT | 1 cut(s) | 586 |
| NspI | RCATGY | 2 cut(s) | 1070, 2603 |
| PaeI | GCATGC | 1 cut(s) | 1070 |
| PaeR7I | CTCGAG | 1 cut(s) | 1820 |
| PagI | TCATGA | 2 cut(s) | 1239, 2647 |
| PciI | ACATGT | 1 cut(s) | 2599 |
| PciSI | GCTCTTC | 2 cut(s) | 829, 1056 |
| PctI | GAATGC | 3 cut(s) | 489, 2395, 2486 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1659 |
| PfoI | TCCNGGA | 1 cut(s) | 1941 |
| PleI | GAGTC | 4 cut(s) | 1251, 2053, 2436, 2569 |
| PpsI | GAGTC | 4 cut(s) | 1251, 2053, 2436, 2569 |
| Ppu21I | YACGTR | 1 cut(s) | 2261 |
| PscI | ACATGT | 1 cut(s) | 2599 |
| PshBI | ATTAAT | 1 cut(s) | 2400 |
| PsiI | TTATAA | 2 cut(s) | 2039, 2373 |
| Psp6I | CCWGG | 4 cut(s) | 319, 2192, 2523, 2546 |
| PspFI | CCCAGC | 1 cut(s) | 595 |
| PspGI | CCWGG | 4 cut(s) | 319, 2192, 2523, 2546 |
| PspN4I | GGNNCC | 4 cut(s) | 771, 1294, 2416, 2462 |
| PspPI | GGNCC | 2 cut(s) | 2550, 2767 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 846, 878 |
| PsuI | RGATCY | 5 cut(s) | 211, 769, 1906, 1945, 2752 |
| PvuII | CAGCTG | 1 cut(s) | 2585 |
| RsaI | GTAC | 4 cut(s) | 864, 914, 2416, 2537 |
| RsaNI | GTAC | 4 cut(s) | 863, 913, 2415, 2536 |
| RseI | CAYNNNNRTG | 5 cut(s) | 685, 1022, 1044, 1543, 2343 |
| SapI | GCTCTTC | 2 cut(s) | 829, 1056 |
| Sau96I | GGNCC | 2 cut(s) | 2550, 2767 |
| SchI | GAGTC | 4 cut(s) | 1252, 2053, 2436, 2569 |
| ScrFI | CCNGG | 7 cut(s) | 321, 1943, 2194, 2525, 2548, 2657, 2682 |
| SduI | GDGCHC | 1 cut(s) | 1366 |
| Sfr274I | CTCGAG | 1 cut(s) | 1820 |
| SinI | GGWCC | 1 cut(s) | 2767 |
| SlaI | CTCGAG | 1 cut(s) | 1820 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 685, 1022, 1044, 1543, 2343 |
| SmlI | CTYRAG | 6 cut(s) | 68, 893, 932, 1247, 1820, 1952 |
| SmoI | CTYRAG | 6 cut(s) | 68, 893, 932, 1247, 1820, 1952 |
| SphI | GCATGC | 1 cut(s) | 1070 |
| SsiI | CCGC | 5 cut(s) | 442, 767, 1296, 2269, 2418 |
| SspI | AATATT | 2 cut(s) | 794, 1414 |
| SspMI | CTAG | 6 cut(s) | 281, 515, 732, 1709, 2543, 2606 |
| StyD4I | CCNGG | 7 cut(s) | 319, 1941, 2192, 2523, 2546, 2655, 2680 |
| StyI | CCWWGG | 2 cut(s) | 1144, 1538 |
| TaaI | ACNGT | 6 cut(s) | 48, 1043, 1074, 2150, 2288, 2690 |
| TaiI | ACGT | 1 cut(s) | 2263 |
| TaqI | TCGA | 6 cut(s) | 481, 1336, 1404, 1653, 1821, 2761 |
| TatI | WGTACW | 3 cut(s) | 862, 912, 2535 |
| TscAI | CASTG | 4 cut(s) | 1048, 2004, 2754, 2786 |
| TseFI | GTSAC | 3 cut(s) | 2056, 2572, 2690 |
| Tsp45I | GTSAC | 3 cut(s) | 2056, 2572, 2690 |
| TspGWI | ACGGA | 1 cut(s) | 1757 |
| TspRI | CASTG | 4 cut(s) | 1048, 2004, 2754, 2786 |
| VpaK11BI | GGWCC | 1 cut(s) | 2767 |
| VspI | ATTAAT | 1 cut(s) | 2400 |
| XapI | RAATTY | 3 cut(s) | 254, 640, 1607 |
| XceI | RCATGY | 2 cut(s) | 1070, 2603 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 614, 1142 |
| XhoI | CTCGAG | 1 cut(s) | 1820 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1659 |
| XspI | CTAG | 6 cut(s) | 281, 515, 732, 1709, 2543, 2606 |
| Zsp2I | ATGCAT | 1 cut(s) | 586 |