Rroxscaffold_7G00164980

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000387
Physical Location & Seq
Reverse (-)
6888821 .. 6891054
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164980.1

Sequence Viewer

Length: 369 bp
ATGCATAAGCAAATCCATACCGTTCCCTCTGCAGATTTTGCACTTTCGGGTGAGTATAGGCCATTGTTGGTGGATAACTTTCATCCCGTCAATTTTTCTTTTAGATTTACGTTTGGGAATGTCTTTGAATTGATTAGGACAAATTTGATGAAGCTTCGTTGTGACTTCAAGGATGCATCTGTTGAGAAGCATGGGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACGCCTTTCAATTGATGATATGGCTGGAGGTTCATTTACAATATCCAATGGTGGATTTTGTGGAAGCCTTATAAGTACCCCCATCATCAACCCTCCTCGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

122

Amino Acids

13.58

Weight (kDa)

9.34

Isoelectric Point (pI)

24.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 74 - 121 6.6e-13 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 335
AcsI RAATTY 1 cut(s) 142
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 1 cut(s) 340
AfiI CCNNNNNNNGG 1 cut(s) 361
AgsI TTSAA 3 cut(s) 128, 169, 273
AluBI AGCT 1 cut(s) 154
AluI AGCT 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 1 cut(s) 59
ApoI RAATTY 1 cut(s) 142
AsuHPI GGTGA 1 cut(s) 62
AvaI CYCGRG 1 cut(s) 360
BccI CCATC 1 cut(s) 353
BfmI CTRYAG 1 cut(s) 30
BmeT110I CYCGRG 1 cut(s) 360
BmsI GCATC 2 cut(s) 163, 185
BpmI CTGGAG 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 359
Bsc4I CCNNNNNNNGG 1 cut(s) 361
BseDI CCNNGG 1 cut(s) 359
BseGI GGATG 2 cut(s) 82, 178
BseLI CCNNNNNNNGG 1 cut(s) 361
BseRI GAGGAG 1 cut(s) 348
BshFI GGCC 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 360
BslFI GGGAC 1 cut(s) 276
BslI CCNNNNNNNGG 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 276
BsnI GGCC 1 cut(s) 61
BsoBI CYCGRG 1 cut(s) 360
Bsp143I GATC 1 cut(s) 231
BspANI GGCC 1 cut(s) 61
BspMAI CTGCAG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 359
BssMI GATC 1 cut(s) 231
BssNI GRCGYC 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 265
BstAPI GCANNNNNTGC 1 cut(s) 38
BstC8I GCNNGC 1 cut(s) 243
BstF5I GGATG 2 cut(s) 82, 178
BstKTI GATC 1 cut(s) 234
BstMBI GATC 1 cut(s) 231
BstMWI GCNNNNNNNGC 2 cut(s) 38, 251
BstSFI CTRYAG 1 cut(s) 30
BsuRI GGCC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 82, 178
Cac8I GCNNGC 1 cut(s) 243
CseI GACGC 1 cut(s) 273
Csp6I GTAC 1 cut(s) 339
CviAII CATG 1 cut(s) 191
CviJI RGCY 5 cut(s) 61, 154, 208, 287, 330
CviKI_1 RGCY 5 cut(s) 61, 154, 208, 287, 330
CviQI GTAC 1 cut(s) 339
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 360
EcoT22I ATGCAT 2 cut(s) 6, 178
FaeI CATG 1 cut(s) 194
FaiI YATR 7 cut(s) 6, 18, 57, 192, 212, 284, 335
FaqI GGGAC 1 cut(s) 276
FatI CATG 1 cut(s) 190
FokI GGATG 2 cut(s) 69, 185
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 1 cut(s) 61
HgaI GACGC 1 cut(s) 273
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 1 cut(s) 194
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 1 cut(s) 219
HphI GGTGA 1 cut(s) 62
Hpy188III TCNNGA 1 cut(s) 216
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 1 cut(s) 110
HpyCH4V TGCA 5 cut(s) 4, 32, 41, 176, 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 251
HpySE526I ACGT 1 cut(s) 110
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 1 cut(s) 194
Kzo9I GATC 1 cut(s) 231
LpnPI CCDG 1 cut(s) 273
LweI GCATC 2 cut(s) 163, 185
MaeII ACGT 1 cut(s) 110
MaeIII GTNAC 1 cut(s) 161
MalI GATC 1 cut(s) 233
MboI GATC 1 cut(s) 231
MfeI CAATTG 1 cut(s) 273
MluCI AATT 5 cut(s) 91, 128, 142, 235, 273
MnlI CCTC 5 cut(s) 37, 253, 284, 366, 369
Mph1103I ATGCAT 2 cut(s) 6, 178
MunI CAATTG 1 cut(s) 273
MwoI GCNNNNNNNGC 2 cut(s) 38, 251
NdeII GATC 1 cut(s) 231
NlaIII CATG 1 cut(s) 194
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 2 cut(s) 6, 178
PfeI GAWTC 1 cut(s) 219
PsiI TTATAA 1 cut(s) 335
PstI CTGCAG 1 cut(s) 34
RsaI GTAC 1 cut(s) 340
RsaNI GTAC 1 cut(s) 339
Sau3AI GATC 1 cut(s) 231
SetI ASST 3 cut(s) 113, 156, 295
SfaNI GCATC 2 cut(s) 163, 185
SfcI CTRYAG 1 cut(s) 30
SgeI CNNG 9 cut(s) 60, 98, 181, 203, 211, 228, 238, 254, 300
Sse9I AATT 5 cut(s) 91, 128, 142, 235, 273
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 1 cut(s) 113
TaqI TCGA 1 cut(s) 215
TasI AATT 5 cut(s) 91, 128, 142, 235, 273
TfiI GAWTC 1 cut(s) 219
TseFI GTSAC 1 cut(s) 161
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 4 cut(s) 71, 164, 211, 285
XapI RAATTY 1 cut(s) 142
Zsp2I ATGCAT 2 cut(s) 6, 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.