2-oxoacid dehydrogenases acyltransferase (catalytic domain)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 20 |
| Acc65I | GGTACC | 1 cut(s) | 998 |
| AccB1I | GGYRCC | 2 cut(s) | 320, 998 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 428 |
| AccII | CGCG | 1 cut(s) | 776 |
| AciI | CCGC | 3 cut(s) | 680, 1131, 1155 |
| AclWI | GGATC | 4 cut(s) | 822, 1141, 1361, 1374 |
| AcoI | YGGCCR | 1 cut(s) | 327 |
| AcsI | RAATTY | 1 cut(s) | 811 |
| AcuI | CTGAAG | 3 cut(s) | 359, 603, 899 |
| AfaI | GTAC | 3 cut(s) | 1000, 1020, 1334 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 299, 428, 838, 1351 |
| AgsI | TTSAA | 5 cut(s) | 228, 389, 754, 793, 1149 |
| AjiI | CACGTC | 1 cut(s) | 1238 |
| AjnI | CCWGG | 3 cut(s) | 346, 360, 475 |
| AluBI | AGCT | 7 cut(s) | 29, 105, 162, 260, 685, 823, 899 |
| AluI | AGCT | 7 cut(s) | 29, 105, 162, 260, 685, 823, 899 |
| Alw21I | GWGCWC | 1 cut(s) | 616 |
| Alw26I | GTCTC | 3 cut(s) | 545, 963, 1175 |
| AlwI | GGATC | 4 cut(s) | 822, 1141, 1361, 1374 |
| Ama87I | CYCGRG | 2 cut(s) | 193, 202 |
| AoxI | GGCC | 2 cut(s) | 248, 327 |
| ApeKI | GCWGC | 4 cut(s) | 29, 89, 682, 899 |
| ApoI | RAATTY | 1 cut(s) | 811 |
| AseI | ATTAAT | 1 cut(s) | 1191 |
| Asp718I | GGTACC | 1 cut(s) | 998 |
| AspLEI | GCGC | 2 cut(s) | 911, 1344 |
| AsuHPI | GGTGA | 4 cut(s) | 362, 412, 639, 1132 |
| AsuNHI | GCTAGC | 1 cut(s) | 416 |
| AvaI | CYCGRG | 2 cut(s) | 193, 202 |
| AxyI | CCTNAGG | 1 cut(s) | 693 |
| BalI | TGGCCA | 1 cut(s) | 329 |
| BamHI | GGATCC | 1 cut(s) | 1366 |
| BanI | GGYRCC | 2 cut(s) | 320, 998 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 28, 60 |
| BauI | CACGAG | 1 cut(s) | 81 |
| BbsI | GAAGAC | 2 cut(s) | 69, 1073 |
| Bbv12I | GWGCWC | 1 cut(s) | 616 |
| BbvI | GCAGC | 4 cut(s) | 16, 76, 694, 886 |
| BccI | CCATC | 5 cut(s) | 234, 311, 649, 1117, 1310 |
| BceAI | ACGGC | 1 cut(s) | 1329 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 62, 96, 174, 208 |
| BciT130I | CCWGG | 3 cut(s) | 348, 362, 477 |
| BciVI | GTATCC | 1 cut(s) | 505 |
| BclI | TGATCA | 1 cut(s) | 1300 |
| BcoDI | GTCTC | 3 cut(s) | 545, 963, 1175 |
| BfaI | CTAG | 4 cut(s) | 102, 417, 449, 1269 |
| BfmI | CTRYAG | 3 cut(s) | 71, 468, 618 |
| BfoI | RGCGCY | 1 cut(s) | 912 |
| BfuI | GTATCC | 1 cut(s) | 505 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1091 |
| BisI | GCNGC | 4 cut(s) | 30, 90, 683, 900 |
| BlsI | GCNGC | 4 cut(s) | 31, 91, 684, 901 |
| Bme1390I | CCNGG | 3 cut(s) | 348, 362, 477 |
| BmeT110I | CYCGRG | 2 cut(s) | 193, 202 |
| BmgBI | CACGTC | 1 cut(s) | 1238 |
| BmiI | GGNNCC | 6 cut(s) | 322, 481, 714, 1000, 1105, 1368 |
| BmrFI | CCNGG | 3 cut(s) | 348, 362, 477 |
| BmsI | GCATC | 6 cut(s) | 101, 148, 207, 527, 832, 928 |
| BmtI | GCTAGC | 1 cut(s) | 420 |
| BpiI | GAAGAC | 2 cut(s) | 69, 1073 |
| BpmI | CTGGAG | 1 cut(s) | 288 |
| BsaAI | YACGTR | 1 cut(s) | 1296 |
| BsaJI | CCNNGG | 3 cut(s) | 276, 324, 483 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 261, 291, 453, 483, 1245, 1275 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 299, 428, 838, 1351 |
| Bse1I | ACTGG | 4 cut(s) | 33, 271, 926, 1022 |
| Bse21I | CCTNAGG | 1 cut(s) | 693 |
| BseBI | CCWGG | 3 cut(s) | 348, 362, 477 |
| BseDI | CCNNGG | 3 cut(s) | 276, 324, 483 |
| BseGI | GGATG | 4 cut(s) | 641, 847, 958, 1360 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 299, 428, 838, 1351 |
| BseMII | CTCAG | 4 cut(s) | 498, 536, 551, 1214 |
| BseNI | ACTGG | 4 cut(s) | 33, 271, 926, 1022 |
| BseRI | GAGGAG | 2 cut(s) | 564, 1188 |
| BseXI | GCAGC | 4 cut(s) | 16, 76, 694, 886 |
| BseYI | CCCAGC | 1 cut(s) | 421 |
| Bsh1236I | CGCG | 1 cut(s) | 776 |
| BshFI | GGCC | 2 cut(s) | 250, 329 |
| BshNI | GGYRCC | 2 cut(s) | 320, 998 |
| BsiHKAI | GWGCWC | 1 cut(s) | 616 |
| BsiHKCI | CYCGRG | 2 cut(s) | 193, 202 |
| BslFI | GGGAC | 1 cut(s) | 1023 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 299, 428, 838, 1351 |
| BsmAI | GTCTC | 3 cut(s) | 545, 963, 1175 |
| BsmFI | GGGAC | 1 cut(s) | 1023 |
| BsnI | GGCC | 2 cut(s) | 250, 329 |
| BsoBI | CYCGRG | 2 cut(s) | 193, 202 |
| Bsp1286I | GDGCHC | 1 cut(s) | 616 |
| Bsp143I | GATC | 5 cut(s) | 827, 1133, 1185, 1300, 1366 |
| BspACI | CCGC | 3 cut(s) | 680, 1131, 1155 |
| BspANI | GGCC | 2 cut(s) | 250, 329 |
| BspCNI | CTCAG | 4 cut(s) | 499, 535, 550, 1213 |
| BspFNI | CGCG | 1 cut(s) | 776 |
| BspLI | GGNNCC | 6 cut(s) | 322, 481, 714, 1000, 1105, 1368 |
| BspMAI | CTGCAG | 1 cut(s) | 75 |
| BspOI | GCTAGC | 1 cut(s) | 420 |
| BspPI | GGATC | 4 cut(s) | 822, 1141, 1361, 1374 |
| BspQI | GCTCTTC | 1 cut(s) | 49 |
| BspT107I | GGYRCC | 2 cut(s) | 320, 998 |
| BsrI | ACTGG | 4 cut(s) | 33, 271, 926, 1022 |
| BssECI | CCNNGG | 3 cut(s) | 276, 324, 483 |
| BssMI | GATC | 5 cut(s) | 827, 1133, 1185, 1300, 1366 |
| BssSI | CACGAG | 1 cut(s) | 81 |
| BssT1I | CCWWGG | 2 cut(s) | 324, 483 |
| Bst2BI | CACGAG | 1 cut(s) | 81 |
| Bst2UI | CCWGG | 3 cut(s) | 348, 362, 477 |
| Bst4CI | ACNGT | 4 cut(s) | 277, 469, 666, 1144 |
| Bst6I | CTCTTC | 1 cut(s) | 49 |
| BstBAI | YACGTR | 1 cut(s) | 1296 |
| BstC8I | GCNNGC | 6 cut(s) | 12, 107, 183, 418, 1129, 1220 |
| BstDEI | CTNAG | 6 cut(s) | 507, 522, 537, 693, 1005, 1200 |
| BstDSI | CCRYGG | 1 cut(s) | 276 |
| BstEII | GGTNACC | 1 cut(s) | 526 |
| BstF5I | GGATG | 4 cut(s) | 641, 847, 958, 1360 |
| BstFNI | CGCG | 1 cut(s) | 776 |
| BstH2I | RGCGCY | 1 cut(s) | 912 |
| BstHHI | GCGC | 2 cut(s) | 911, 1344 |
| BstKTI | GATC | 5 cut(s) | 830, 1136, 1188, 1303, 1369 |
| BstMAI | GTCTC | 3 cut(s) | 545, 963, 1175 |
| BstMBI | GATC | 5 cut(s) | 827, 1133, 1185, 1300, 1366 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 182, 371, 422, 620, 908, 1091 |
| BstNI | CCWGG | 3 cut(s) | 348, 362, 477 |
| BstNSI | RCATGY | 1 cut(s) | 1222 |
| BstPI | GGTNACC | 1 cut(s) | 526 |
| BstSCI | CCNGG | 3 cut(s) | 346, 360, 475 |
| BstSFI | CTRYAG | 3 cut(s) | 71, 468, 618 |
| BstSNI | TACGTA | 1 cut(s) | 1296 |
| BstUI | CGCG | 1 cut(s) | 776 |
| BstV1I | GCAGC | 4 cut(s) | 16, 76, 694, 886 |
| BstV2I | GAAGAC | 2 cut(s) | 69, 1073 |
| BstX2I | RGATCY | 1 cut(s) | 1366 |
| BstYI | RGATCY | 1 cut(s) | 1366 |
| Bsu36I | CCTNAGG | 1 cut(s) | 693 |
| BsuI | GTATCC | 1 cut(s) | 505 |
| BsuRI | GGCC | 2 cut(s) | 250, 329 |
| BtgI | CCRYGG | 1 cut(s) | 276 |
| BtrI | CACGTC | 1 cut(s) | 1238 |
| BtsCI | GGATG | 4 cut(s) | 641, 847, 958, 1360 |
| BtsI | GCAGTG | 1 cut(s) | 68 |
| BtsIMutI | CAGTG | 1 cut(s) | 68 |
| Cac8I | GCNNGC | 6 cut(s) | 12, 107, 183, 418, 1129, 1220 |
| CfoI | GCGC | 2 cut(s) | 911, 1344 |
| CsiI | ACCWGGT | 1 cut(s) | 346 |
| Csp6I | GTAC | 3 cut(s) | 999, 1019, 1333 |
| CviAII | CATG | 3 cut(s) | 152, 860, 1219 |
| CviQI | GTAC | 3 cut(s) | 999, 1019, 1333 |
| DdeI | CTNAG | 6 cut(s) | 507, 522, 537, 693, 1005, 1200 |
| DpnI | GATC | 5 cut(s) | 829, 1135, 1187, 1302, 1368 |
| DpnII | GATC | 5 cut(s) | 827, 1133, 1185, 1300, 1366 |
| EaeI | YGGCCR | 1 cut(s) | 327 |
| Eam1104I | CTCTTC | 1 cut(s) | 49 |
| EarI | CTCTTC | 1 cut(s) | 49 |
| EciI | GGCGGA | 1 cut(s) | 1146 |
| Eco105I | TACGTA | 1 cut(s) | 1296 |
| Eco130I | CCWWGG | 2 cut(s) | 324, 483 |
| Eco147I | AGGCCT | 1 cut(s) | 250 |
| Eco32I | GATATC | 2 cut(s) | 958, 982 |
| Eco57I | CTGAAG | 3 cut(s) | 359, 603, 899 |
| Eco81I | CCTNAGG | 1 cut(s) | 693 |
| Eco88I | CYCGRG | 2 cut(s) | 193, 202 |
| Eco91I | GGTNACC | 1 cut(s) | 526 |
| EcoO65I | GGTNACC | 1 cut(s) | 526 |
| EcoRII | CCWGG | 3 cut(s) | 346, 360, 475 |
| EcoRV | GATATC | 2 cut(s) | 958, 982 |
| EcoT14I | CCWWGG | 2 cut(s) | 324, 483 |
| EcoT22I | ATGCAT | 1 cut(s) | 847 |
| ErhI | CCWWGG | 2 cut(s) | 324, 483 |
| FaeI | CATG | 3 cut(s) | 155, 863, 1222 |
| FaqI | GGGAC | 1 cut(s) | 1023 |
| FatI | CATG | 3 cut(s) | 151, 859, 1218 |
| FauNDI | CATATG | 1 cut(s) | 297 |
| FbaI | TGATCA | 1 cut(s) | 1300 |
| Fnu4HI | GCNGC | 4 cut(s) | 30, 90, 683, 900 |
| FokI | GGATG | 4 cut(s) | 628, 854, 965, 1367 |
| Fsp4HI | GCNGC | 4 cut(s) | 30, 90, 683, 900 |
| FspBI | CTAG | 4 cut(s) | 102, 417, 449, 1269 |
| GlaI | GCGC | 2 cut(s) | 910, 1343 |
| GluI | GCNGC | 4 cut(s) | 30, 90, 683, 900 |
| GsaI | CCCAGC | 1 cut(s) | 425 |
| GsuI | CTGGAG | 1 cut(s) | 288 |
| HaeII | RGCGCY | 1 cut(s) | 912 |
| HaeIII | GGCC | 2 cut(s) | 250, 329 |
| HhaI | GCGC | 2 cut(s) | 911, 1344 |
| Hin1II | CATG | 3 cut(s) | 155, 863, 1222 |
| Hin6I | GCGC | 2 cut(s) | 909, 1342 |
| HinP1I | GCGC | 2 cut(s) | 909, 1342 |
| HincII | GTYRAC | 1 cut(s) | 454 |
| HindII | GTYRAC | 1 cut(s) | 454 |
| HinfI | GANTC | 3 cut(s) | 305, 357, 758 |
| HphI | GGTGA | 4 cut(s) | 362, 412, 639, 1132 |
| Hpy166II | GTNNAC | 2 cut(s) | 454, 1333 |
| Hpy188I | TCNGA | 8 cut(s) | 121, 339, 508, 540, 717, 732, 827, 1185 |
| Hpy188III | TCNNGA | 3 cut(s) | 202, 885, 1385 |
| Hpy8I | GTNNAC | 2 cut(s) | 454, 1333 |
| HpyAV | CCTTC | 6 cut(s) | 60, 452, 579, 923, 1084, 1094 |
| HpyCH4III | ACNGT | 4 cut(s) | 277, 469, 666, 1144 |
| HpyCH4IV | ACGT | 2 cut(s) | 1237, 1295 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 182, 371, 422, 620, 908, 1091 |
| HpyF3I | CTNAG | 6 cut(s) | 507, 522, 537, 693, 1005, 1200 |
| HpySE526I | ACGT | 2 cut(s) | 1237, 1295 |
| Hsp92II | CATG | 3 cut(s) | 155, 863, 1222 |
| HspAI | GCGC | 2 cut(s) | 909, 1342 |
| KpnI | GGTACC | 1 cut(s) | 1002 |
| Ksp22I | TGATCA | 1 cut(s) | 1300 |
| Kzo9I | GATC | 5 cut(s) | 827, 1133, 1185, 1300, 1366 |
| LguI | GCTCTTC | 1 cut(s) | 49 |
| LmnI | GCTCC | 2 cut(s) | 619, 828 |
| Lsp1109I | GCAGC | 4 cut(s) | 16, 76, 694, 886 |
| LweI | GCATC | 6 cut(s) | 101, 148, 207, 527, 832, 928 |
| MabI | ACCWGGT | 1 cut(s) | 346 |
| MaeI | CTAG | 4 cut(s) | 102, 417, 449, 1269 |
| MaeII | ACGT | 2 cut(s) | 1237, 1295 |
| MaeIII | GTNAC | 3 cut(s) | 400, 526, 1233 |
| MalI | GATC | 5 cut(s) | 829, 1135, 1187, 1302, 1368 |
| MboI | GATC | 5 cut(s) | 827, 1133, 1185, 1300, 1366 |
| MboII | GAAGA | 9 cut(s) | 36, 69, 87, 216, 352, 553, 596, 1078, 1373 |
| MflI | RGATCY | 1 cut(s) | 1366 |
| MhlI | GDGCHC | 1 cut(s) | 616 |
| MlsI | TGGCCA | 1 cut(s) | 329 |
| MluCI | AATT | 3 cut(s) | 309, 384, 811 |
| MluNI | TGGCCA | 1 cut(s) | 329 |
| MlyI | GAGTC | 2 cut(s) | 366, 752 |
| Mox20I | TGGCCA | 1 cut(s) | 329 |
| Mph1103I | ATGCAT | 1 cut(s) | 847 |
| MscI | TGGCCA | 1 cut(s) | 329 |
| MseI | TTAA | 2 cut(s) | 894, 1191 |
| Msp20I | TGGCCA | 1 cut(s) | 329 |
| MspA1I | CMGCKG | 1 cut(s) | 260 |
| MspR9I | CCNGG | 3 cut(s) | 348, 362, 477 |
| MvaI | CCWGG | 3 cut(s) | 348, 362, 477 |
| MvnI | CGCG | 1 cut(s) | 776 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 182, 371, 422, 620, 908, 1091 |
| NdeI | CATATG | 1 cut(s) | 297 |
| NdeII | GATC | 5 cut(s) | 827, 1133, 1185, 1300, 1366 |
| NheI | GCTAGC | 1 cut(s) | 416 |
| NlaIII | CATG | 3 cut(s) | 155, 863, 1222 |
| NlaIV | GGNNCC | 6 cut(s) | 322, 481, 714, 1000, 1105, 1368 |
| NmeAIII | GCCGAG | 2 cut(s) | 1081, 1194 |
| NmuCI | GTSAC | 2 cut(s) | 400, 1233 |
| NsiI | ATGCAT | 1 cut(s) | 847 |
| NspI | RCATGY | 1 cut(s) | 1222 |
| PaeI | GCATGC | 1 cut(s) | 1222 |
| PaeR7I | CTCGAG | 1 cut(s) | 202 |
| PceI | AGGCCT | 1 cut(s) | 250 |
| PciSI | GCTCTTC | 1 cut(s) | 49 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 200, 209 |
| PfeI | GAWTC | 1 cut(s) | 305 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 428 |
| PkrI | GCNGC | 4 cut(s) | 31, 91, 684, 901 |
| PleI | GAGTC | 2 cut(s) | 365, 752 |
| PpsI | GAGTC | 2 cut(s) | 365, 752 |
| Ppu21I | YACGTR | 1 cut(s) | 1296 |
| PshBI | ATTAAT | 1 cut(s) | 1191 |
| PsiI | TTATAA | 1 cut(s) | 20 |
| Psp6I | CCWGG | 3 cut(s) | 346, 360, 475 |
| PspEI | GGTNACC | 1 cut(s) | 526 |
| PspFI | CCCAGC | 1 cut(s) | 421 |
| PspGI | CCWGG | 3 cut(s) | 346, 360, 475 |
| PspN4I | GGNNCC | 6 cut(s) | 322, 481, 714, 1000, 1105, 1368 |
| PstI | CTGCAG | 1 cut(s) | 75 |
| PsuI | RGATCY | 1 cut(s) | 1366 |
| PvuII | CAGCTG | 1 cut(s) | 260 |
| RsaI | GTAC | 3 cut(s) | 1000, 1020, 1334 |
| RsaNI | GTAC | 3 cut(s) | 999, 1019, 1333 |
| SapI | GCTCTTC | 1 cut(s) | 49 |
| SaqAI | TTAA | 2 cut(s) | 894, 1191 |
| SatI | GCNGC | 4 cut(s) | 30, 90, 683, 900 |
| Sau3AI | GATC | 5 cut(s) | 827, 1133, 1185, 1300, 1366 |
| SchI | GAGTC | 2 cut(s) | 366, 752 |
| ScrFI | CCNGG | 3 cut(s) | 348, 362, 477 |
| SduI | GDGCHC | 1 cut(s) | 616 |
| SexAI | ACCWGGT | 1 cut(s) | 346 |
| SfaNI | GCATC | 6 cut(s) | 101, 148, 207, 527, 832, 928 |
| SfcI | CTRYAG | 3 cut(s) | 71, 468, 618 |
| Sfr274I | CTCGAG | 1 cut(s) | 202 |
| SlaI | CTCGAG | 1 cut(s) | 202 |
| SmlI | CTYRAG | 1 cut(s) | 202 |
| SmoI | CTYRAG | 1 cut(s) | 202 |
| SnaBI | TACGTA | 1 cut(s) | 1296 |
| SphI | GCATGC | 1 cut(s) | 1222 |
| Sse9I | AATT | 3 cut(s) | 309, 384, 811 |
| SseBI | AGGCCT | 1 cut(s) | 250 |
| SsiI | CCGC | 3 cut(s) | 680, 1131, 1155 |
| SspMI | CTAG | 4 cut(s) | 102, 417, 449, 1269 |
| StuI | AGGCCT | 1 cut(s) | 250 |
| StyD4I | CCNGG | 3 cut(s) | 346, 360, 475 |
| StyI | CCWWGG | 2 cut(s) | 324, 483 |
| TaaI | ACNGT | 4 cut(s) | 277, 469, 666, 1144 |
| TaiI | ACGT | 2 cut(s) | 1240, 1298 |
| TaqI | TCGA | 2 cut(s) | 203, 1226 |
| TasI | AATT | 3 cut(s) | 309, 384, 811 |
| TatI | WGTACW | 1 cut(s) | 1332 |
| TfiI | GAWTC | 1 cut(s) | 305 |
| Tru1I | TTAA | 2 cut(s) | 894, 1191 |
| Tru9I | TTAA | 2 cut(s) | 894, 1191 |
| TscAI | CASTG | 1 cut(s) | 75 |
| TseFI | GTSAC | 2 cut(s) | 400, 1233 |
| TseI | GCWGC | 4 cut(s) | 29, 89, 682, 899 |
| Tsp45I | GTSAC | 2 cut(s) | 400, 1233 |
| TspDTI | ATGAA | 6 cut(s) | 155, 810, 833, 867, 1061, 1113 |
| TspRI | CASTG | 1 cut(s) | 75 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 428 |
| VspI | ATTAAT | 1 cut(s) | 1191 |
| XapI | RAATTY | 1 cut(s) | 811 |
| XceI | RCATGY | 1 cut(s) | 1222 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1120 |
| XhoI | CTCGAG | 1 cut(s) | 202 |
| XspI | CTAG | 4 cut(s) | 102, 417, 449, 1269 |
| Zsp2I | ATGCAT | 1 cut(s) | 847 |