RchiOBHm_Chr5g0052431

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000387
Physical Location & Seq
Forward (+)
54284772 .. 54287039
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32983

Sequence Viewer

Length: 393 bp
ATGAAGCTTCATTGTGACTTCAAGGATGCATCTGTTGAGAAGCATGGGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGGCTGTTGTCAGTGGGGTCCAAAATCAGCCTACTATTAATGCAGTCATTGATGGGGATGATATCATATATAGAGATTACATAGATATAAGTGCAGCTGTGGGTACTCCAAAGGGCCTTGTTGTGCCAGTTATCCGCAATGCTGATAAGATGAATTGTGGTGAGATTGAGAAGGAGATCAATTTGCTTGCAAAGAAGGCAAATGAGGGACGTTTTTCAATTGATGATATGGCTGGAGGTTCATTTACAATATCCAATGGTGGATTTTATGGAAGCCTTATAAGTACCCCCATCATCAACCCTCCTCATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

130

Amino Acids

13.99

Weight (kDa)

6.27

Isoelectric Point (pI)

24.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 5 - 130 9.4e-40 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 359
AciI CCGC 1 cut(s) 214
AfaI GTAC 2 cut(s) 184, 364
AgsI TTSAA 2 cut(s) 22, 297
AluBI AGCT 2 cut(s) 7, 176
AluI AGCT 2 cut(s) 7, 176
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 1 cut(s) 173
AseI ATTAAT 1 cut(s) 117
AspS9I GGNCC 2 cut(s) 97, 193
AsuHPI GGTGA 1 cut(s) 251
AvaII GGWCC 1 cut(s) 97
BbvI GCAGC 1 cut(s) 185
BccI CCATC 2 cut(s) 125, 377
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 97, 193
BmiI GGNNCC 1 cut(s) 98
BmsI GCATC 2 cut(s) 16, 38
BpmI CTGGAG 1 cut(s) 333
Bse1I ACTGG 1 cut(s) 206
Bse3DI GCAATG 1 cut(s) 223
BseGI GGATG 2 cut(s) 31, 142
BseMI GCAATG 1 cut(s) 223
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 1 cut(s) 372
BseXI GCAGC 1 cut(s) 185
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 1 cut(s) 195
BslFI GGGAC 1 cut(s) 300
BsmFI GGGAC 1 cut(s) 300
BsnI GGCC 1 cut(s) 195
Bsp143I GATC 1 cut(s) 255
BspACI CCGC 1 cut(s) 214
BspANI GGCC 1 cut(s) 195
BspLI GGNNCC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 223
BsrI ACTGG 1 cut(s) 206
BssMI GATC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 267
BstF5I GGATG 2 cut(s) 31, 142
BstKTI GATC 1 cut(s) 258
BstMBI GATC 1 cut(s) 255
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstV1I GCAGC 1 cut(s) 185
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 2 cut(s) 31, 142
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 267
Cfr13I GGNCC 2 cut(s) 97, 193
Csp6I GTAC 2 cut(s) 183, 363
CviAII CATG 2 cut(s) 44, 386
CviJI RGCY 8 cut(s) 7, 61, 83, 109, 176, 195, 311, 354
CviKI_1 RGCY 8 cut(s) 7, 61, 83, 109, 176, 195, 311, 354
CviQI GTAC 2 cut(s) 183, 363
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
Eco32I GATATC 1 cut(s) 142
Eco47I GGWCC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 193
EcoRV GATATC 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 31
FaeI CATG 2 cut(s) 47, 389
FaqI GGGAC 1 cut(s) 300
FatI CATG 2 cut(s) 43, 385
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 2 cut(s) 38, 149
Fsp4HI GCNGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 1 cut(s) 195
Hin1II CATG 2 cut(s) 47, 389
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 1 cut(s) 72
HphI GGTGA 1 cut(s) 251
Hpy188I TCNGA 1 cut(s) 392
Hpy188III TCNNGA 1 cut(s) 69
HpyAV CCTTC 2 cut(s) 244, 268
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 4 cut(s) 29, 122, 173, 269
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 2 cut(s) 47, 389
Kzo9I GATC 1 cut(s) 255
LpnPI CCDG 2 cut(s) 219, 297
Lsp1109I GCAGC 1 cut(s) 185
LweI GCATC 2 cut(s) 16, 38
MaeII ACGT 1 cut(s) 289
MaeIII GTNAC 1 cut(s) 14
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MfeI CAATTG 1 cut(s) 297
MluCI AATT 3 cut(s) 232, 259, 297
MnlI CCTC 4 cut(s) 277, 308, 390, 393
Mph1103I ATGCAT 1 cut(s) 31
MseI TTAA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 176
MunI CAATTG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 275
NdeII GATC 1 cut(s) 255
NlaIII CATG 2 cut(s) 47, 389
NlaIV GGNNCC 1 cut(s) 98
NmuCI GTSAC 1 cut(s) 14
NsiI ATGCAT 1 cut(s) 31
PfeI GAWTC 1 cut(s) 72
PkrI GCNGC 1 cut(s) 175
PshBI ATTAAT 1 cut(s) 117
PsiI TTATAA 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 98
PspPI GGNCC 2 cut(s) 97, 193
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 2 cut(s) 184, 364
RsaNI GTAC 2 cut(s) 183, 363
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 1 cut(s) 255
Sau96I GGNCC 2 cut(s) 97, 193
SetI ASST 4 cut(s) 9, 178, 292, 319
SfaNI GCATC 2 cut(s) 16, 38
SgeI CNNG 9 cut(s) 34, 56, 64, 81, 91, 209, 218, 278, 324
SinI GGWCC 1 cut(s) 97
Sse9I AATT 3 cut(s) 232, 259, 297
SsiI CCGC 1 cut(s) 214
TaiI ACGT 1 cut(s) 292
TaqI TCGA 1 cut(s) 68
TasI AATT 3 cut(s) 232, 259, 297
TfiI GAWTC 1 cut(s) 72
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 4 cut(s) 17, 64, 245, 309
TspRI CASTG 1 cut(s) 97
VpaK11BI GGWCC 1 cut(s) 97
VspI ATTAAT 1 cut(s) 117
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.