RLG00000031115

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000387
Physical Location & Seq
Reverse (-)
2253690 .. 2256020
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031115

Sequence Viewer

Length: 573 bp
ATGGGAGACTGGGAGGTGACCAGATTCGATTTAAGCAAAGGGAGAAAGGCAGAAAACCAACGCATAAGCAAATCCATACCTGTTCCCTCTGAAGATTTTGCACTTGCGGGTGAGTATAGGTCATTGTTGGTGGATAACTTTCATCCTGTCAATTTTTCTTTTAGATTTACGTTTGGGAATGTCTTTGAATTGATTAGGACAAATTTGATGAAGCTTCGTTGTGACTTCAAGGATGCATCTGTTGAGAATCATGGGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGTCTACTATTAATGCAGTCATTGATGGGGATGATATCATATATAGAGATTACATAGATATAAGTGCAGCTATGGGTACTCCAAAGGGCCTTGTTGTGCCAGTTATCCGCAATGCTGATAAGATGAATTGTGGTGAGATTGAGAAGGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACGTCTTTCAATTGATGATATGGCTGGAGGTTCATTTACAATATCCAATGGTGGATTTTATGGAAGCCTTATAAGTACCCCCATCATCAACCCTCCTCAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

191

Amino Acids

21.06

Weight (kDa)

6.14

Isoelectric Point (pI)

24.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 63 - 190 1.2e-34 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AatII GACGTC 1 cut(s) 472
AccI GTMKAC 1 cut(s) 290
AciI CCGC 2 cut(s) 107, 394
AcsI RAATTY 1 cut(s) 202
AcuI CTGAAG 1 cut(s) 111
AcyI GRCGYC 1 cut(s) 469
AfaI GTAC 2 cut(s) 364, 544
AfiI CCNNNNNNNGG 1 cut(s) 565
AgsI TTSAA 3 cut(s) 188, 229, 477
AluBI AGCT 2 cut(s) 214, 356
AluI AGCT 2 cut(s) 214, 356
AoxI GGCC 1 cut(s) 373
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 1 cut(s) 202
AseI ATTAAT 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 373
AsuHPI GGTGA 3 cut(s) 28, 122, 431
AxyI CCTNAGG 1 cut(s) 564
BbvI GCAGC 1 cut(s) 365
BccI CCATC 2 cut(s) 305, 557
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
BmgT120I GGNCC 1 cut(s) 373
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 2 cut(s) 223, 245
BmuI ACTGGG 1 cut(s) 19
BpmI CTGGAG 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 469
Bsc4I CCNNNNNNNGG 1 cut(s) 565
Bse1I ACTGG 2 cut(s) 14, 386
Bse21I CCTNAGG 1 cut(s) 564
Bse3DI GCAATG 1 cut(s) 403
BseGI GGATG 3 cut(s) 142, 238, 322
BseLI CCNNNNNNNGG 1 cut(s) 565
BseMI GCAATG 1 cut(s) 403
BseNI ACTGG 2 cut(s) 14, 386
BseRI GAGGAG 1 cut(s) 552
BseXI GCAGC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 1 cut(s) 375
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 565
BsmFI GGGAC 1 cut(s) 480
BsnI GGCC 1 cut(s) 375
Bsp143I GATC 1 cut(s) 435
BspACI CCGC 2 cut(s) 107, 394
BspANI GGCC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 403
BsrI ACTGG 2 cut(s) 14, 386
BssMI GATC 1 cut(s) 435
BssNI GRCGYC 1 cut(s) 469
BstACI GRCGYC 1 cut(s) 469
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 1 cut(s) 564
BstEII GGTNACC 1 cut(s) 16
BstF5I GGATG 3 cut(s) 142, 238, 322
BstKTI GATC 1 cut(s) 438
BstMBI GATC 1 cut(s) 435
BstMWI GCNNNNNNNGC 1 cut(s) 455
BstPI GGTNACC 1 cut(s) 16
BstV1I GCAGC 1 cut(s) 365
Bsu36I CCTNAGG 1 cut(s) 564
BsuRI GGCC 1 cut(s) 375
BtsCI GGATG 3 cut(s) 142, 238, 322
Cac8I GCNNGC 1 cut(s) 447
Cfr13I GGNCC 1 cut(s) 373
Csp6I GTAC 2 cut(s) 363, 543
CviAII CATG 1 cut(s) 251
CviJI RGCY 6 cut(s) 214, 268, 356, 375, 491, 534
CviKI_1 RGCY 6 cut(s) 214, 268, 356, 375, 491, 534
CviQI GTAC 2 cut(s) 363, 543
DdeI CTNAG 1 cut(s) 564
DpnI GATC 1 cut(s) 437
DpnII GATC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 111
Eco81I CCTNAGG 1 cut(s) 564
Eco91I GGTNACC 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 373
EcoO65I GGTNACC 1 cut(s) 16
EcoRV GATATC 1 cut(s) 322
EcoT22I ATGCAT 1 cut(s) 238
FaeI CATG 1 cut(s) 254
FaqI GGGAC 1 cut(s) 480
FatI CATG 1 cut(s) 250
FauI CCCGC 1 cut(s) 100
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 1 cut(s) 354
FokI GGATG 3 cut(s) 129, 245, 329
Fsp4HI GCNGC 1 cut(s) 354
GluI GCNGC 1 cut(s) 354
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 469
Hin1II CATG 1 cut(s) 254
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 3 cut(s) 24, 247, 279
HphI GGTGA 3 cut(s) 28, 122, 431
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 2 cut(s) 91, 572
Hpy188III TCNNGA 1 cut(s) 276
Hpy8I GTNNAC 1 cut(s) 291
HpyAV CCTTC 2 cut(s) 424, 448
HpyCH4IV ACGT 2 cut(s) 170, 469
HpyCH4V TGCA 5 cut(s) 101, 236, 302, 353, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 455
HpyF3I CTNAG 1 cut(s) 564
HpySE526I ACGT 2 cut(s) 170, 469
Hsp92I GRCGYC 1 cut(s) 469
Hsp92II CATG 1 cut(s) 254
Kzo9I GATC 1 cut(s) 435
LpnPI CCDG 6 cut(s) 34, 93, 159, 399, 477, 551
Lsp1109I GCAGC 1 cut(s) 365
LweI GCATC 2 cut(s) 223, 245
MaeII ACGT 2 cut(s) 170, 469
MaeIII GTNAC 2 cut(s) 16, 221
MalI GATC 1 cut(s) 437
MboI GATC 1 cut(s) 435
MboII GAAGA 1 cut(s) 104
MfeI CAATTG 1 cut(s) 477
MluCI AATT 6 cut(s) 151, 188, 202, 412, 439, 477
MnlI CCTC 6 cut(s) 7, 97, 457, 488, 570, 573
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 2 cut(s) 32, 297
MunI CAATTG 1 cut(s) 477
MwoI GCNNNNNNNGC 1 cut(s) 455
NdeII GATC 1 cut(s) 435
NlaIII CATG 1 cut(s) 254
NmuCI GTSAC 2 cut(s) 16, 221
NsiI ATGCAT 1 cut(s) 238
PfeI GAWTC 3 cut(s) 24, 247, 279
PkrI GCNGC 1 cut(s) 355
PshBI ATTAAT 1 cut(s) 297
PsiI TTATAA 1 cut(s) 539
PspEI GGTNACC 1 cut(s) 16
PspPI GGNCC 1 cut(s) 373
RsaI GTAC 2 cut(s) 364, 544
RsaNI GTAC 2 cut(s) 363, 543
SaqAI TTAA 2 cut(s) 32, 297
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 1 cut(s) 435
Sau96I GGNCC 1 cut(s) 373
SetI ASST 9 cut(s) 18, 82, 122, 173, 216, 358, 472, 499, 570
SfaNI GCATC 2 cut(s) 223, 245
Sse9I AATT 6 cut(s) 151, 188, 202, 412, 439, 477
SsiI CCGC 2 cut(s) 107, 394
TaiI ACGT 2 cut(s) 173, 472
TaqI TCGA 2 cut(s) 27, 275
TasI AATT 6 cut(s) 151, 188, 202, 412, 439, 477
TfiI GAWTC 3 cut(s) 24, 247, 279
Tru1I TTAA 2 cut(s) 32, 297
Tru9I TTAA 2 cut(s) 32, 297
TseFI GTSAC 2 cut(s) 16, 221
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 2 cut(s) 16, 221
TspDTI ATGAA 5 cut(s) 131, 224, 271, 425, 489
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 290
ZraI GACGTC 1 cut(s) 470
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.