Rh5BG355700

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000387
Physical Location & Seq
Reverse (-)
55170106 .. 55172823
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG355700.1

Sequence Viewer

Length: 804 bp
ATGGCGGGTAAGACCGACTCGGTGTCGTACTGTAACCTGAAGGATACGAAAGTTGAAATGATGGGGATGGATGCTGAGCCTCATCATCGGATCGACCTCAATTCCTCTAGGCTGTCTCCATCGCAGTCGTCGTCGTCCTTTGCTAGGGTTCCGAACGGGAACTACGATCTTCGGCCGCCGCAGAGTCTACCGGTTCCTAATCAGAACACTCTGCTGACTCTAATTCTCAGCTGTACGGTTGCCGCCGGTGTTCAGGGTGACGTGGGAGACTGGGAGGTGACCAGATTCGATTTAAGCAAAGGGAGAAAGGCAGAAAACCAACGCATAAGCAAATCCATACCTGTTCCTTCTGAAGATTTTGCACTTTCGGGCAACCGCAACGACAAGAATGAGAAAAATGAGACAAATTTGATGAAGCTTCATTGTGACTTCAAGGATGCATCTGTTGAGAAGCATGGGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGGCTGTTGTCAGTGGGGTCCAAAATCAGCCTACTATTAATGCAGTCATTGATGGGGATGATATCATATATAGAGATTACATAGATATAAGTGCAGCTGTGGGTACTCCAAAGGGCCTTGTTGTGCCAGTTATCCGCAATGCTGATAAGATGAATTGTGGTGAGATTGAGAAGGAGATCAATTTGCTTGCAAAGAAGGCAAATGAGGGACGTTTTTCAATTGATGATATGGCTGGAGGTTCATTTACAATATCCAATGGTGGATTTTATGGAAGCCTTATAAGTACCCCCATCATCAACCCTCCTCATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

267

Amino Acids

29.01

Weight (kDa)

6.22

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 132 - 267 1.3e-40 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 770
AccI GTMKAC 1 cut(s) 187
AciI CCGC 6 cut(s) 5, 176, 179, 243, 376, 625
AclWI GGATC 1 cut(s) 98
AcoI YGGCCR 1 cut(s) 173
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 2 cut(s) 59, 372
AfaI GTAC 4 cut(s) 29, 235, 595, 775
AfiI CCNNNNNNNGG 1 cut(s) 144
AgeI ACCGGT 1 cut(s) 190
AgsI TTSAA 3 cut(s) 56, 433, 708
AhdI GACNNNNNGTC 1 cut(s) 22
AjiI CACGTC 1 cut(s) 262
AluBI AGCT 3 cut(s) 231, 418, 587
AluI AGCT 3 cut(s) 231, 418, 587
Alw26I GTCTC 3 cut(s) 120, 261, 395
AlwI GGATC 1 cut(s) 98
AoxI GGCC 2 cut(s) 173, 604
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 1 cut(s) 406
AseI ATTAAT 1 cut(s) 528
AsiGI ACCGGT 1 cut(s) 190
AspS9I GGNCC 2 cut(s) 508, 604
AsuHPI GGTGA 3 cut(s) 269, 289, 662
AvaII GGWCC 1 cut(s) 508
BaeI ACNNNNGTAYC 2 cut(s) 36, 69
BbvI GCAGC 1 cut(s) 596
BccI CCATC 5 cut(s) 55, 61, 127, 536, 788
BciVI GTATCC 1 cut(s) 37
BcoDI GTCTC 3 cut(s) 120, 261, 395
BfaI CTAG 2 cut(s) 108, 144
BfuI GTATCC 1 cut(s) 37
BisI GCNGC 4 cut(s) 176, 179, 243, 585
BlpI GCTNAGC 1 cut(s) 75
BlsI GCNGC 4 cut(s) 177, 180, 244, 586
Bme18I GGWCC 1 cut(s) 508
BmeRI GACNNNNNGTC 1 cut(s) 22
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 508, 604
BmiI GGNNCC 3 cut(s) 150, 195, 509
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 3 cut(s) 61, 427, 449
BmuI ACTGGG 1 cut(s) 280
BpmI CTGGAG 1 cut(s) 744
Bpu1102I GCTNAGC 1 cut(s) 75
BsaWI WCCGGW 1 cut(s) 190
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse118I RCCGGY 2 cut(s) 190, 245
Bse1I ACTGG 2 cut(s) 275, 617
Bse3DI GCAATG 1 cut(s) 634
BseGI GGATG 4 cut(s) 72, 76, 442, 553
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMI GCAATG 1 cut(s) 634
BseMII CTCAG 2 cut(s) 66, 241
BseNI ACTGG 2 cut(s) 275, 617
BseRI GAGGAG 1 cut(s) 783
BseX3I CGGCCG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 596
BsgI GTGCAG 1 cut(s) 603
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 2 cut(s) 175, 606
BshTI ACCGGT 1 cut(s) 190
BsiEI CGRYCG 1 cut(s) 176
BsiSI CCGG 2 cut(s) 191, 246
BslFI GGGAC 1 cut(s) 711
BslI CCNNNNNNNGG 1 cut(s) 144
BsmAI GTCTC 3 cut(s) 120, 261, 395
BsmFI GGGAC 1 cut(s) 711
BsnI GGCC 2 cut(s) 175, 606
Bsp143I GATC 3 cut(s) 90, 166, 666
Bsp1720I GCTNAGC 1 cut(s) 75
BspACI CCGC 6 cut(s) 5, 176, 179, 243, 376, 625
BspANI GGCC 2 cut(s) 175, 606
BspCNI CTCAG 2 cut(s) 67, 240
BspLI GGNNCC 3 cut(s) 150, 195, 509
BspPI GGATC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 634
BsrFI RCCGGY 2 cut(s) 190, 245
BsrI ACTGG 2 cut(s) 275, 617
BssAI RCCGGY 2 cut(s) 190, 245
BssMI GATC 3 cut(s) 90, 166, 666
Bst4CI ACNGT 2 cut(s) 32, 238
BstC8I GCNNGC 1 cut(s) 678
BstDEI CTNAG 2 cut(s) 75, 227
BstEII GGTNACC 1 cut(s) 277
BstENI CCTNNNNNAGG 1 cut(s) 142
BstF5I GGATG 4 cut(s) 72, 76, 442, 553
BstKTI GATC 3 cut(s) 93, 169, 669
BstMAI GTCTC 3 cut(s) 120, 261, 395
BstMBI GATC 3 cut(s) 90, 166, 666
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 1 cut(s) 686
BstPI GGTNACC 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 596
BstZI CGGCCG 1 cut(s) 173
BsuI GTATCC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 175, 606
BtgZI GCGATG 1 cut(s) 105
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 4 cut(s) 72, 76, 442, 553
BtsIMutI CAGTG 1 cut(s) 508
Cac8I GCNNGC 1 cut(s) 678
Cfr10I RCCGGY 2 cut(s) 190, 245
Cfr13I GGNCC 2 cut(s) 508, 604
Csp6I GTAC 4 cut(s) 28, 234, 594, 774
CspAI ACCGGT 1 cut(s) 190
CviAII CATG 2 cut(s) 455, 797
CviQI GTAC 4 cut(s) 28, 234, 594, 774
DdeI CTNAG 2 cut(s) 75, 227
DpnI GATC 3 cut(s) 92, 168, 668
DpnII GATC 3 cut(s) 90, 166, 666
DriI GACNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 173
EagI CGGCCG 1 cut(s) 173
Eam1105I GACNNNNNGTC 1 cut(s) 22
EclXI CGGCCG 1 cut(s) 173
Eco32I GATATC 1 cut(s) 553
Eco47I GGWCC 1 cut(s) 508
Eco52I CGGCCG 1 cut(s) 173
Eco57I CTGAAG 2 cut(s) 59, 372
Eco91I GGTNACC 1 cut(s) 277
EcoNI CCTNNNNNAGG 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 604
EcoO65I GGTNACC 1 cut(s) 277
EcoRV GATATC 1 cut(s) 553
EcoT22I ATGCAT 1 cut(s) 442
FaeI CATG 2 cut(s) 458, 800
FaqI GGGAC 1 cut(s) 711
FatI CATG 2 cut(s) 454, 796
FblI GTMKAC 1 cut(s) 187
Fnu4HI GCNGC 4 cut(s) 176, 179, 243, 585
FokI GGATG 4 cut(s) 79, 83, 449, 560
Fsp4HI GCNGC 4 cut(s) 176, 179, 243, 585
FspBI CTAG 2 cut(s) 108, 144
GluI GCNGC 4 cut(s) 176, 179, 243, 585
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 2 cut(s) 175, 606
HapII CCGG 2 cut(s) 191, 246
Hin1II CATG 2 cut(s) 458, 800
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 5 cut(s) 17, 184, 217, 285, 483
HpaII CCGG 2 cut(s) 191, 246
HphI GGTGA 3 cut(s) 269, 289, 662
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 5 cut(s) 90, 153, 204, 352, 803
Hpy188III TCNNGA 1 cut(s) 480
Hpy8I GTNNAC 1 cut(s) 188
Hpy99I CGWCG 2 cut(s) 133, 136
HpyAV CCTTC 4 cut(s) 34, 357, 655, 679
HpyCH4III ACNGT 2 cut(s) 32, 238
HpyCH4IV ACGT 2 cut(s) 261, 700
HpyCH4V TGCA 5 cut(s) 362, 440, 533, 584, 680
HpyF10VI GCNNNNNNNGC 1 cut(s) 686
HpyF3I CTNAG 2 cut(s) 75, 227
HpySE526I ACGT 2 cut(s) 261, 700
Hsp92II CATG 2 cut(s) 458, 800
Kzo9I GATC 3 cut(s) 90, 166, 666
LpnPI CCDG 9 cut(s) 50, 204, 239, 256, 259, 295, 354, 630, 708
Lsp1109I GCAGC 1 cut(s) 596
LweI GCATC 3 cut(s) 61, 427, 449
MaeI CTAG 2 cut(s) 108, 144
MaeII ACGT 2 cut(s) 261, 700
MaeIII GTNAC 4 cut(s) 32, 257, 277, 425
MalI GATC 3 cut(s) 92, 168, 668
MboI GATC 3 cut(s) 90, 166, 666
MboII GAAGA 2 cut(s) 161, 365
MfeI CAATTG 1 cut(s) 708
MluCI AATT 6 cut(s) 100, 222, 406, 643, 670, 708
MlyI GAGTC 3 cut(s) 11, 193, 211
MnlI CCTC 8 cut(s) 90, 107, 115, 268, 688, 719, 801, 804
Mph1103I ATGCAT 1 cut(s) 442
MseI TTAA 2 cut(s) 293, 528
MspA1I CMGCKG 2 cut(s) 231, 587
MspI CCGG 2 cut(s) 191, 246
MunI CAATTG 1 cut(s) 708
MwoI GCNNNNNNNGC 1 cut(s) 686
NdeII GATC 3 cut(s) 90, 166, 666
NlaIII CATG 2 cut(s) 458, 800
NlaIV GGNNCC 3 cut(s) 150, 195, 509
NmuCI GTSAC 3 cut(s) 257, 277, 425
NsiI ATGCAT 1 cut(s) 442
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 162
PfeI GAWTC 2 cut(s) 285, 483
PinAI ACCGGT 1 cut(s) 190
PkrI GCNGC 4 cut(s) 177, 180, 244, 586
PleI GAGTC 3 cut(s) 11, 192, 211
PpsI GAGTC 3 cut(s) 11, 192, 211
PshBI ATTAAT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 770
PspEI GGTNACC 1 cut(s) 277
PspN4I GGNNCC 3 cut(s) 150, 195, 509
PspPI GGNCC 2 cut(s) 508, 604
PsrI GAACNNNNNNTAC 2 cut(s) 146, 178
PvuII CAGCTG 2 cut(s) 231, 587
RsaI GTAC 4 cut(s) 29, 235, 595, 775
RsaNI GTAC 4 cut(s) 28, 234, 594, 774
SaqAI TTAA 2 cut(s) 293, 528
SatI GCNGC 4 cut(s) 176, 179, 243, 585
Sau3AI GATC 3 cut(s) 90, 166, 666
Sau96I GGNCC 2 cut(s) 508, 604
SchI GAGTC 3 cut(s) 11, 193, 211
SfaNI GCATC 3 cut(s) 61, 427, 449
SgrAI CRCCGGYG 1 cut(s) 245
SinI GGWCC 1 cut(s) 508
Sse9I AATT 6 cut(s) 100, 222, 406, 643, 670, 708
SsiI CCGC 6 cut(s) 5, 176, 179, 243, 376, 625
SspMI CTAG 2 cut(s) 108, 144
TaaI ACNGT 2 cut(s) 32, 238
TaiI ACGT 2 cut(s) 264, 703
TaqI TCGA 3 cut(s) 93, 288, 479
TaqII GACCGA 1 cut(s) 29
TasI AATT 6 cut(s) 100, 222, 406, 643, 670, 708
TauI GCSGC 3 cut(s) 178, 181, 245
TfiI GAWTC 2 cut(s) 285, 483
Tru1I TTAA 2 cut(s) 293, 528
Tru9I TTAA 2 cut(s) 293, 528
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 3 cut(s) 257, 277, 425
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 3 cut(s) 257, 277, 425
TspDTI ATGAA 5 cut(s) 410, 428, 475, 656, 720
TspRI CASTG 1 cut(s) 508
VpaK11BI GGWCC 1 cut(s) 508
VspI ATTAAT 1 cut(s) 528
XagI CCTNNNNNAGG 1 cut(s) 142
XapI RAATTY 1 cut(s) 406
XmiI GTMKAC 1 cut(s) 187
XspI CTAG 2 cut(s) 108, 144
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.