Rw5G030020

Large subunit GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000387
Physical Location & Seq
Forward (+)
47181918 .. 47193744
11827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030020.1

Sequence Viewer

Length: 1041 bp
ATGCCTGGAGACCTGGTGGAAGTTGATGAAGCAATTGCTCAAATTGAAACAGATAAGGTCATGATCGATGTTGCTAGTCCTGAATCCGGTGTAATTGTGAAGTTTGTAGTCAATAAAGGGGATACTATGGAACCAAGCACCAAGATTGTTGTCATACTAAAGTCCAATGAAAGTGTATCCCATGTGGGTCCATCTGAGAAAACATTGGAGAAAGCTGTTCCACAACCAGCTTCCCCTGTAGAAACAATTGGAAGAATCAAACATCTAAAGTTGAAAAATTTGATGAAGCTTCGTTGTGACTTCAAGGATGCATCTGTTGAGAAGCATGAGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGGGCCTTGTTGTGCCAGTTATCTGCAATGCTGATAAGATGAATTGTGGTGAGATAGAGAAGGAAATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACGTCTTTCAATTGATGATATGATTCAATGGCATCATCTAGAATGTGAAGATGCTAATTATGTCTCCAAGAACCAATATAGCACCATCTTCTTGCTCCATTTTCCATCATACAAGACCTGGGCAAACGAACAGCCTAACCCTCCCAACGTGTTCAATTTGTACACGGAAAGCCACCGTCGTCTTCAAGCCGCTTCGAAACCACAGAGAGAGAGCAAAAATGCCGACAAGAACGAGAAGATGAAGCTGGGAAGAGCCGTGGTGCGGCAGAACAACCAGTTGATTCGGCAGACGAAAGAGAAAGGCATCATGTACAGAAGGATGCAGAAGAAGGTTCTAGAATCCGTCACGGAGGTCAGCGACATCGACGCCGTAATCGAACAGGCCGACCGCCCCTTCTCCCTCAACAACCCTAAACCCAATCTTCTCATCAACCTGGACGGGAGTGAGATAACCCCGCCGGAGCAAAGGAGGGAGCAGCAGAGGAAGGAGGAGGCCTTGCAAGCCGGTAGTCTCCAGGTGCCACGTAGGCCGCCGTGGACTCCAGAAATGTCAGTGGAGGAGCTCGACGACAAAGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000166 GO:0001882 GO:0001883 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005783 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009790 GO:0009791 GO:0009793 GO:0009966 GO:0009987 GO:0010154 GO:0010467 GO:0010646 GO:0010817 GO:0012505 GO:0015030 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016072 GO:0016462 GO:0016604 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0022414 GO:0022613 GO:0023051 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032879 GO:0033036 GO:0033750 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035639 GO:0036094 GO:0042254 GO:0042886 GO:0043021 GO:0043022 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046626 GO:0046907 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048583 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051049 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061458 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0090069 GO:0090070 GO:0090304 GO:0097159 GO:0097367 GO:0099402 GO:1900076 GO:1901265 GO:1901360 GO:1901363 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

346

Amino Acids

39.31

Weight (kDa)

7.07

Isoelectric Point (pI)

50.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 2 - 52 7e-10 Biotin-requiring enzyme
2-oxoacid_dh PF00198 95 - 160 2.4e-10 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 976
AciI CCGC 5 cut(s) 648, 721, 847, 914, 989
AcsI RAATTY 1 cut(s) 277
AcyI GRCGYC 2 cut(s) 460, 825
AfaI GTAC 2 cut(s) 620, 770
AfiI CCNNNNNNNGG 2 cut(s) 86, 720
AflIII ACRYGT 1 cut(s) 606
AgsI TTSAA 7 cut(s) 47, 274, 304, 468, 485, 613, 644
AjnI CCWGG 5 cut(s) 4, 12, 575, 891, 972
AluBI AGCT 5 cut(s) 215, 230, 289, 703, 1021
AluI AGCT 5 cut(s) 215, 230, 289, 703, 1021
Alw21I GWGCWC 1 cut(s) 1023
Alw26I GTCTC 3 cut(s) 3, 526, 974
AoxI GGCC 4 cut(s) 364, 840, 951, 986
ApeKI GCWGC 1 cut(s) 934
ApoI RAATTY 1 cut(s) 277
AspS9I GGNCC 2 cut(s) 188, 364
AsuHPI GGTGA 1 cut(s) 422
AsuII TTCGAA 1 cut(s) 653
AvaII GGWCC 1 cut(s) 188
BanI GGYRCC 1 cut(s) 976
BanII GRGCYC 1 cut(s) 1023
BbsI GAAGAC 1 cut(s) 632
Bbv12I GWGCWC 1 cut(s) 1023
BbvI GCAGC 1 cut(s) 946
BccI CCATC 3 cut(s) 199, 551, 571
BceAI ACGGC 3 cut(s) 698, 812, 976
BciT130I CCWGG 5 cut(s) 6, 14, 577, 893, 974
BciVI GTATCC 2 cut(s) 115, 187
BcoDI GTCTC 3 cut(s) 3, 526, 974
BfaI CTAG 3 cut(s) 75, 497, 794
BfmI CTRYAG 1 cut(s) 237
BfuI GTATCC 2 cut(s) 115, 187
BglI GCCNNNNNGGC 1 cut(s) 985
BisI GCNGC 4 cut(s) 648, 722, 935, 989
BlsI GCNGC 4 cut(s) 649, 723, 936, 990
Bme1390I CCNGG 5 cut(s) 6, 14, 577, 893, 974
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 188, 364
BmiI GGNNCC 3 cut(s) 132, 189, 978
BmrFI CCNGG 5 cut(s) 6, 14, 577, 893, 974
BmsI GCATC 6 cut(s) 298, 320, 499, 499, 768, 771
BpiI GAAGAC 1 cut(s) 632
BpmI CTGGAG 3 cut(s) 27, 956, 984
Bpu14I TTCGAA 1 cut(s) 653
Bsa29I ATCGAT 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 983
BsaHI GRCGYC 2 cut(s) 460, 825
BsaI GGTCTC 1 cut(s) 3
BsaJI CCNNGG 3 cut(s) 576, 714, 992
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 720
Bse118I RCCGGY 1 cut(s) 962
Bse1I ACTGG 2 cut(s) 377, 733
Bse3DI GCAATG 1 cut(s) 394
BseBI CCWGG 5 cut(s) 6, 14, 577, 893, 974
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 3 cut(s) 576, 714, 992
BseGI GGATG 2 cut(s) 313, 783
BseLI CCNNNNNNNGG 2 cut(s) 86, 720
BseMI GCAATG 1 cut(s) 394
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 2 cut(s) 377, 733
BseRI GAGGAG 2 cut(s) 962, 1031
BseXI GCAGC 1 cut(s) 946
BseYI CCCAGC 1 cut(s) 703
Bsh1285I CGRYCG 1 cut(s) 847
BshFI GGCC 4 cut(s) 366, 842, 953, 988
BshNI GGYRCC 1 cut(s) 976
BshVI ATCGAT 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 847
BsiHKAI GWGCWC 1 cut(s) 1023
BsiSI CCGG 3 cut(s) 87, 917, 963
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 2 cut(s) 86, 720
BsmAI GTCTC 3 cut(s) 3, 526, 974
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 4 cut(s) 366, 842, 953, 988
Bso31I GGTCTC 1 cut(s) 3
Bsp119I TTCGAA 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 1023
Bsp1407I TGTACA 2 cut(s) 618, 768
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 5 cut(s) 648, 721, 847, 914, 989
BspANI GGCC 4 cut(s) 366, 842, 953, 988
BspCNI CTCAG 1 cut(s) 187
BspDI ATCGAT 1 cut(s) 66
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 3 cut(s) 132, 189, 978
BspQI GCTCTTC 1 cut(s) 703
BspT104I TTCGAA 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 976
BspTNI GGTCTC 1 cut(s) 3
BsrDI GCAATG 1 cut(s) 394
BsrFI RCCGGY 1 cut(s) 962
BsrGI TGTACA 2 cut(s) 618, 768
BsrI ACTGG 2 cut(s) 377, 733
BssAI RCCGGY 1 cut(s) 962
BssECI CCNNGG 3 cut(s) 576, 714, 992
BssMI GATC 1 cut(s) 63
BssNI GRCGYC 2 cut(s) 460, 825
Bst2UI CCWGG 5 cut(s) 6, 14, 577, 893, 974
Bst4CI ACNGT 1 cut(s) 635
Bst6I CTCTTC 1 cut(s) 703
BstACI GRCGYC 2 cut(s) 460, 825
BstAUI TGTACA 2 cut(s) 618, 768
BstBAI YACGTR 1 cut(s) 983
BstBI TTCGAA 1 cut(s) 653
BstC8I GCNNGC 2 cut(s) 438, 960
BstDEI CTNAG 1 cut(s) 195
BstDSI CCRYGG 2 cut(s) 714, 992
BstF5I GGATG 2 cut(s) 313, 783
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 3 cut(s) 3, 526, 974
BstMBI GATC 1 cut(s) 63
BstMCI CGRYCG 1 cut(s) 847
BstMWI GCNNNNNNNGC 3 cut(s) 446, 959, 985
BstNI CCWGG 5 cut(s) 6, 14, 577, 893, 974
BstSCI CCNGG 5 cut(s) 4, 12, 575, 891, 972
BstSFI CTRYAG 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 946
BstV2I GAAGAC 1 cut(s) 632
Bsu15I ATCGAT 1 cut(s) 66
BsuI GTATCC 2 cut(s) 115, 187
BsuRI GGCC 4 cut(s) 366, 842, 953, 988
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 2 cut(s) 714, 992
BtsCI GGATG 2 cut(s) 313, 783
BtsIMutI CAGTG 1 cut(s) 1017
Cac8I GCNNGC 2 cut(s) 438, 960
CciI TCATGA 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 962
Cfr13I GGNCC 2 cut(s) 188, 364
ClaI ATCGAT 1 cut(s) 66
CseI GACGC 1 cut(s) 833
CsiI ACCWGGT 1 cut(s) 12
Csp6I GTAC 2 cut(s) 619, 769
CviAII CATG 4 cut(s) 61, 182, 326, 766
CviQI GTAC 2 cut(s) 619, 769
DdeI CTNAG 1 cut(s) 195
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 703
EarI CTCTTC 1 cut(s) 703
Ecl136II GAGCTC 1 cut(s) 1021
Eco147I AGGCCT 1 cut(s) 953
Eco24I GRGCYC 1 cut(s) 1023
Eco31I GGTCTC 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 1021
EcoICRI GAGCTC 1 cut(s) 1021
EcoO109I RGGNCCY 1 cut(s) 364
EcoRII CCWGG 5 cut(s) 4, 12, 575, 891, 972
EcoT22I ATGCAT 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 1023
FaeI CATG 4 cut(s) 64, 185, 329, 769
FaqI GGGAC 1 cut(s) 471
FatI CATG 4 cut(s) 60, 181, 325, 765
FauI CCCGC 1 cut(s) 921
Fnu4HI GCNGC 4 cut(s) 648, 722, 935, 989
FokI GGATG 2 cut(s) 320, 790
FriOI GRGCYC 1 cut(s) 1023
Fsp4HI GCNGC 4 cut(s) 648, 722, 935, 989
FspBI CTAG 3 cut(s) 75, 497, 794
GluI GCNGC 4 cut(s) 648, 722, 935, 989
GsaI CCCAGC 1 cut(s) 707
GsuI CTGGAG 3 cut(s) 27, 956, 984
HaeIII GGCC 4 cut(s) 366, 842, 953, 988
HapII CCGG 3 cut(s) 87, 917, 963
HgaI GACGC 1 cut(s) 833
Hin1I GRCGYC 2 cut(s) 460, 825
Hin1II CATG 4 cut(s) 64, 185, 329, 769
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 7 cut(s) 83, 255, 354, 481, 739, 797, 997
HpaII CCGG 3 cut(s) 87, 917, 963
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 2 cut(s) 621, 996
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 6 cut(s) 61, 80, 351, 497, 794, 1001
Hpy8I GTNNAC 2 cut(s) 621, 996
Hpy99I CGWCG 3 cut(s) 639, 827, 1028
HpyAV CCTTC 6 cut(s) 415, 439, 768, 781, 862, 937
HpyCH4III ACNGT 1 cut(s) 635
HpyCH4IV ACGT 3 cut(s) 460, 606, 982
HpyCH4V TGCA 5 cut(s) 311, 387, 440, 781, 958
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 959, 985
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 3 cut(s) 460, 606, 982
Hsp92I GRCGYC 2 cut(s) 460, 825
Hsp92II CATG 4 cut(s) 64, 185, 329, 769
Kzo9I GATC 1 cut(s) 63
LguI GCTCTTC 1 cut(s) 703
LmnI GCTCC 4 cut(s) 558, 919, 931, 1018
Lsp1109I GCAGC 1 cut(s) 946
LweI GCATC 6 cut(s) 298, 320, 499, 499, 768, 771
MabI ACCWGGT 1 cut(s) 12
MaeI CTAG 3 cut(s) 75, 497, 794
MaeII ACGT 3 cut(s) 460, 606, 982
MaeIII GTNAC 2 cut(s) 296, 802
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 8 cut(s) 264, 518, 538, 632, 706, 720, 796, 872
MfeI CAATTG 3 cut(s) 33, 246, 468
MhlI GDGCHC 1 cut(s) 1023
MlyI GAGTC 1 cut(s) 991
Mph1103I ATGCAT 1 cut(s) 313
MspI CCGG 3 cut(s) 87, 917, 963
MspR9I CCNGG 5 cut(s) 6, 14, 577, 893, 974
MunI CAATTG 3 cut(s) 33, 246, 468
MvaI CCWGG 5 cut(s) 6, 14, 577, 893, 974
MwoI GCNNNNNNNGC 3 cut(s) 446, 959, 985
NdeII GATC 1 cut(s) 63
NlaIII CATG 4 cut(s) 64, 185, 329, 769
NlaIV GGNNCC 3 cut(s) 132, 189, 978
NmuCI GTSAC 2 cut(s) 296, 802
NsiI ATGCAT 1 cut(s) 313
NspV TTCGAA 1 cut(s) 653
PagI TCATGA 1 cut(s) 60
PceI AGGCCT 1 cut(s) 953
PciSI GCTCTTC 1 cut(s) 703
PcsI WCGNNNNNNNCGW 2 cut(s) 831, 840
PfeI GAWTC 6 cut(s) 83, 255, 354, 481, 739, 797
PkrI GCNGC 4 cut(s) 649, 723, 936, 990
PleI GAGTC 1 cut(s) 991
PpsI GAGTC 1 cut(s) 991
Ppu21I YACGTR 1 cut(s) 983
Psp124BI GAGCTC 1 cut(s) 1023
Psp6I CCWGG 5 cut(s) 4, 12, 575, 891, 972
PspFI CCCAGC 1 cut(s) 703
PspGI CCWGG 5 cut(s) 4, 12, 575, 891, 972
PspN4I GGNNCC 3 cut(s) 132, 189, 978
PspPI GGNCC 2 cut(s) 188, 364
RsaI GTAC 2 cut(s) 620, 770
RsaNI GTAC 2 cut(s) 619, 769
SacI GAGCTC 1 cut(s) 1023
SapI GCTCTTC 1 cut(s) 703
SatI GCNGC 4 cut(s) 648, 722, 935, 989
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 2 cut(s) 188, 364
SchI GAGTC 1 cut(s) 991
ScrFI CCNGG 5 cut(s) 6, 14, 577, 893, 974
SduI GDGCHC 1 cut(s) 1023
SexAI ACCWGGT 1 cut(s) 12
SfaNI GCATC 6 cut(s) 298, 320, 499, 499, 768, 771
SfcI CTRYAG 1 cut(s) 237
SfuI TTCGAA 1 cut(s) 653
SinI GGWCC 1 cut(s) 188
SseBI AGGCCT 1 cut(s) 953
SsiI CCGC 5 cut(s) 648, 721, 847, 914, 989
SspMI CTAG 3 cut(s) 75, 497, 794
SstI GAGCTC 1 cut(s) 1023
StuI AGGCCT 1 cut(s) 953
StyD4I CCNGG 5 cut(s) 4, 12, 575, 891, 972
TaaI ACNGT 1 cut(s) 635
TaiI ACGT 3 cut(s) 463, 609, 985
TaqI TCGA 6 cut(s) 66, 350, 653, 822, 834, 1023
TatI WGTACW 2 cut(s) 618, 768
TauI GCSGC 3 cut(s) 650, 724, 991
TfiI GAWTC 6 cut(s) 83, 255, 354, 481, 739, 797
TscAI CASTG 1 cut(s) 1017
TseFI GTSAC 2 cut(s) 296, 802
TseI GCWGC 1 cut(s) 934
Tsp45I GTSAC 2 cut(s) 296, 802
TspDTI ATGAA 6 cut(s) 42, 183, 299, 346, 416, 713
TspGWI ACGGA 3 cut(s) 638, 790, 821
TspRI CASTG 1 cut(s) 1017
VpaK11BI GGWCC 1 cut(s) 188
XapI RAATTY 1 cut(s) 277
XbaI TCTAGA 2 cut(s) 496, 793
XspI CTAG 3 cut(s) 75, 497, 794
ZraI GACGTC 1 cut(s) 461
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.