Rh7CG344900

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000387
Physical Location & Seq
Reverse (-)
38913238 .. 38915499
2262 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG344900.1

Sequence Viewer

Length: 606 bp
ATGGATAGTGGATCTTCTTATTACTTTTCCCATGAGCCTGGAGACCTGGGGGAAGTTGATGAACCAATTGCTCAAATTGAAACAGATAAGGTCACGATCGATGTTGCTAGTTCTGAATCCGGTGTAATTGTGAAGAAATGGATTGCTAAAAGATTGAAAGATTCACAGAATACATATGCAATGCTTACAACATTCAGTGAAGTTGATATAACAAATTTGATGAAGCTTCGTTGTGACTTCAAGGATGCATCTGTTGAGAAGCATGGGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGGCTGCTTTCAGTGGGCTCCAAAATCAGCCTACTATTAATGCAGTCATTGATGGGGATGATATCATATATAGAGATTACATAGATCCAGCTGTGGGTACTCCAAAGGGCCTTGTTGTGCCAGTTATCCGCAATGATGATAAGATGAATTGTGGTGAGATTGAGAAGGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACGTCTTTCAATTGATGATATGGCTGGAGGTTCATTTACAATATCCAATGGTGGATTTTATGGAAGCCTTATAAGTACCCCCATCATCAACCCTCTTCAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

201

Amino Acids

21.91

Weight (kDa)

4.8

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 44 - 201 5.7e-45 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 572
AatII GACGTC 1 cut(s) 505
AciI CCGC 1 cut(s) 427
AclWI GGATC 2 cut(s) 19, 377
AcsI RAATTY 1 cut(s) 214
AcuI CTGAAG 1 cut(s) 581
AcyI GRCGYC 1 cut(s) 502
AfaI GTAC 2 cut(s) 397, 577
AfiI CCNNNNNNNGG 2 cut(s) 392, 598
AgsI TTSAA 4 cut(s) 80, 157, 241, 510
AjnI CCWGG 2 cut(s) 37, 45
AluBI AGCT 2 cut(s) 226, 389
AluI AGCT 2 cut(s) 226, 389
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 2 cut(s) 19, 377
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 1 cut(s) 302
ApoI RAATTY 1 cut(s) 214
AseI ATTAAT 1 cut(s) 336
AspS9I GGNCC 1 cut(s) 406
AsuHPI GGTGA 1 cut(s) 464
BanII GRGCYC 1 cut(s) 318
BbvI GCAGC 1 cut(s) 289
BccI CCATC 2 cut(s) 344, 590
BciT130I CCWGG 2 cut(s) 39, 47
BcoDI GTCTC 1 cut(s) 36
BfaI CTAG 1 cut(s) 108
BisI GCNGC 1 cut(s) 303
BlsI GCNGC 1 cut(s) 304
Bme1390I CCNGG 2 cut(s) 39, 47
BmgT120I GGNCC 1 cut(s) 406
BmiI GGNNCC 1 cut(s) 317
BmrFI CCNGG 2 cut(s) 39, 47
BmsI GCATC 2 cut(s) 235, 257
BpmI CTGGAG 2 cut(s) 60, 546
Bsa29I ATCGAT 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 502
BsaI GGTCTC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 598
Bse1I ACTGG 1 cut(s) 419
Bse3DI GCAATG 2 cut(s) 186, 436
BseBI CCWGG 2 cut(s) 39, 47
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 46
BseGI GGATG 2 cut(s) 250, 361
BseLI CCNNNNNNNGG 2 cut(s) 392, 598
BseMI GCAATG 2 cut(s) 186, 436
BseNI ACTGG 1 cut(s) 419
BseXI GCAGC 1 cut(s) 289
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 408
BshVI ATCGAT 1 cut(s) 99
BsiEI CGRYCG 1 cut(s) 99
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 2 cut(s) 392, 598
BsmAI GTCTC 1 cut(s) 36
BsmFI GGGAC 1 cut(s) 513
BsnI GGCC 1 cut(s) 408
Bso31I GGTCTC 1 cut(s) 36
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 4 cut(s) 11, 96, 382, 468
BspACI CCGC 1 cut(s) 427
BspANI GGCC 1 cut(s) 408
BspDI ATCGAT 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 317
BspPI GGATC 2 cut(s) 19, 377
BspTNI GGTCTC 1 cut(s) 36
BsrDI GCAATG 2 cut(s) 186, 436
BsrI ACTGG 1 cut(s) 419
BssECI CCNNGG 1 cut(s) 46
BssMI GATC 4 cut(s) 11, 96, 382, 468
BssNI GRCGYC 1 cut(s) 502
Bst2UI CCWGG 2 cut(s) 39, 47
Bst6I CTCTTC 1 cut(s) 600
BstACI GRCGYC 1 cut(s) 502
BstC8I GCNNGC 1 cut(s) 480
BstF5I GGATG 2 cut(s) 250, 361
BstKTI GATC 4 cut(s) 14, 99, 385, 471
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 4 cut(s) 11, 96, 382, 468
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 2 cut(s) 39, 47
BstSCI CCNGG 2 cut(s) 37, 45
BstV1I GCAGC 1 cut(s) 289
BstX2I RGATCY 2 cut(s) 11, 382
BstXI CCANNNNNNTGG 1 cut(s) 38
BstYI RGATCY 2 cut(s) 11, 382
Bsu15I ATCGAT 1 cut(s) 99
BsuRI GGCC 1 cut(s) 408
BsuTUI ATCGAT 1 cut(s) 99
BtsCI GGATG 2 cut(s) 250, 361
BtsIMutI CAGTG 2 cut(s) 202, 316
Cac8I GCNNGC 1 cut(s) 480
Cfr13I GGNCC 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 99
Csp6I GTAC 2 cut(s) 396, 576
CviAII CATG 2 cut(s) 32, 263
CviQI GTAC 2 cut(s) 396, 576
DpnI GATC 4 cut(s) 13, 98, 384, 470
DpnII GATC 4 cut(s) 11, 96, 382, 468
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
Eco24I GRGCYC 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 36
Eco32I GATATC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 406
EcoRII CCWGG 2 cut(s) 37, 45
EcoRV GATATC 1 cut(s) 361
EcoT22I ATGCAT 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 318
FaeI CATG 2 cut(s) 35, 266
FaqI GGGAC 1 cut(s) 513
FatI CATG 2 cut(s) 31, 262
FauNDI CATATG 1 cut(s) 175
Fnu4HI GCNGC 1 cut(s) 303
FokI GGATG 2 cut(s) 257, 368
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 1 cut(s) 303
FspBI CTAG 1 cut(s) 108
GluI GCNGC 1 cut(s) 303
GsuI CTGGAG 2 cut(s) 60, 546
HaeIII GGCC 1 cut(s) 408
HapII CCGG 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 502
Hin1II CATG 2 cut(s) 35, 266
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 3 cut(s) 116, 161, 291
HpaII CCGG 1 cut(s) 120
HphI GGTGA 1 cut(s) 464
Hpy188I TCNGA 2 cut(s) 115, 605
Hpy188III TCNNGA 2 cut(s) 94, 288
HpyAV CCTTC 2 cut(s) 457, 481
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 4 cut(s) 179, 248, 341, 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpySE526I ACGT 1 cut(s) 502
Hsp92I GRCGYC 1 cut(s) 502
Hsp92II CATG 2 cut(s) 35, 266
Kzo9I GATC 4 cut(s) 11, 96, 382, 468
LmnI GCTCC 1 cut(s) 321
LpnPI CCDG 9 cut(s) 24, 32, 51, 59, 133, 399, 432, 510, 584
Lsp1109I GCAGC 1 cut(s) 289
LweI GCATC 2 cut(s) 235, 257
MaeI CTAG 1 cut(s) 108
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 2 cut(s) 91, 233
MalI GATC 4 cut(s) 13, 98, 384, 470
MboI GATC 4 cut(s) 11, 96, 382, 468
MboII GAAGA 3 cut(s) 6, 145, 587
MfeI CAATTG 2 cut(s) 66, 510
MflI RGATCY 2 cut(s) 11, 382
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 7 cut(s) 66, 75, 126, 214, 445, 472, 510
MnlI CCTC 3 cut(s) 490, 521, 603
Mph1103I ATGCAT 1 cut(s) 250
MseI TTAA 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 2 cut(s) 39, 47
MunI CAATTG 2 cut(s) 66, 510
MvaI CCWGG 2 cut(s) 39, 47
MwoI GCNNNNNNNGC 1 cut(s) 488
NdeI CATATG 1 cut(s) 175
NdeII GATC 4 cut(s) 11, 96, 382, 468
NlaIII CATG 2 cut(s) 35, 266
NlaIV GGNNCC 1 cut(s) 317
NmuCI GTSAC 2 cut(s) 91, 233
NsiI ATGCAT 1 cut(s) 250
PfeI GAWTC 3 cut(s) 116, 161, 291
PkrI GCNGC 1 cut(s) 304
Ple19I CGATCG 1 cut(s) 99
PshBI ATTAAT 1 cut(s) 336
PsiI TTATAA 1 cut(s) 572
Psp6I CCWGG 2 cut(s) 37, 45
PspGI CCWGG 2 cut(s) 37, 45
PspN4I GGNNCC 1 cut(s) 317
PspPI GGNCC 1 cut(s) 406
PsuI RGATCY 2 cut(s) 11, 382
PvuI CGATCG 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 2 cut(s) 397, 577
RsaNI GTAC 2 cut(s) 396, 576
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 1 cut(s) 303
Sau3AI GATC 4 cut(s) 11, 96, 382, 468
Sau96I GGNCC 1 cut(s) 406
ScrFI CCNGG 2 cut(s) 39, 47
SduI GDGCHC 1 cut(s) 318
SetI ASST 7 cut(s) 48, 93, 228, 391, 505, 532, 603
SfaNI GCATC 2 cut(s) 235, 257
Sse9I AATT 7 cut(s) 66, 75, 126, 214, 445, 472, 510
SsiI CCGC 1 cut(s) 427
SspMI CTAG 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 37, 45
TaiI ACGT 1 cut(s) 505
TaqI TCGA 2 cut(s) 99, 287
TasI AATT 7 cut(s) 66, 75, 126, 214, 445, 472, 510
TfiI GAWTC 3 cut(s) 116, 161, 291
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 2 cut(s) 202, 316
TseFI GTSAC 2 cut(s) 91, 233
TseI GCWGC 1 cut(s) 302
Tsp45I GTSAC 2 cut(s) 91, 233
TspDTI ATGAA 5 cut(s) 75, 236, 283, 458, 522
TspRI CASTG 2 cut(s) 202, 316
VspI ATTAAT 1 cut(s) 336
XapI RAATTY 1 cut(s) 214
XspI CTAG 1 cut(s) 108
ZraI GACGTC 1 cut(s) 503
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.