Rroxscaffold_2G00122640

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000387
Physical Location & Seq
Reverse (-)
55985388 .. 55988169
2782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00122640.1

Sequence Viewer

Length: 735 bp
ATGTATGGAGAATTGAGGTTCGAGTCGCCGACGAGTTACCGAGTCACCGATCAAGTCGCGATGGCGGGTAAGACCGACTCGGTGTCGTACCGCAACTTGAAGGATACGAAAGTTGAAATGATTGGGATGGATGATGAGCCTCATCATCGGATCGACCTCAATTCCTCTAGGCCGTCTCCATCGCCGTCGTCGTCGTCCTCCGCTCGGGTTCCGAACCGGAAAGGCGATCTTCCGCCGCCACGAAGTTTACCGGTTCCTAATCGAACACTCGATGACTCTAATTCTCAACCGTATGGTTGCCGCCGGTGTTCGGTTCGGTTGGGCTTTGCGCTTTCGCTCTTAACTCCCTATATACAATTTACAAAGTGCTTGGCTTTTGAATTTATTTCTAGCCGATTCGGACCCAATTGTTCAGATAAAGTTGAAATTTGTTTGTTGCATAGGACAAATTTGATGAAGCTTCATTGTGACTTCAAGGATGCATCTGTTGAGATGCATGGGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGGGCCTTGTTGTTCCAGTTATCCGCAACGCTAGTAAGATGAATTGTGGTGAGATTGAGAAGGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACGCCTTTCAATTGATGATATGGCTGGAGGTTCATTTACAATATCCAATGGTGGATTTTGTGGAAGCCTTATAAGTACCCCCATCATCAACCCTCCTCGTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

244

Amino Acids

26.9

Weight (kDa)

9.03

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 162 - 243 9e-23 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 701
AccBSI CCGCTC 1 cut(s) 203
AccII CGCG 1 cut(s) 59
AciI CCGC 7 cut(s) 65, 91, 201, 233, 236, 301, 556
AclWI GGATC 1 cut(s) 158
AcsI RAATTY 3 cut(s) 380, 426, 448
AcyI GRCGYC 1 cut(s) 631
AfaI GTAC 2 cut(s) 89, 706
AfiI CCNNNNNNNGG 2 cut(s) 204, 310
AgeI ACCGGT 1 cut(s) 250
AgsI TTSAA 6 cut(s) 100, 116, 380, 425, 475, 639
AhdI GACNNNNNGTC 1 cut(s) 82
AluBI AGCT 1 cut(s) 460
AluI AGCT 1 cut(s) 460
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 2 cut(s) 170, 535
ApoI RAATTY 3 cut(s) 380, 426, 448
AsiGI ACCGGT 1 cut(s) 250
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 2 cut(s) 401, 535
AsuHPI GGTGA 2 cut(s) 37, 593
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 1 cut(s) 401
BaeI ACNNNNGTAYC 2 cut(s) 96, 129
BauI CACGAG 1 cut(s) 726
BccI CCATC 4 cut(s) 55, 121, 187, 719
BceAI ACGGC 2 cut(s) 157, 169
BciVI GTATCC 1 cut(s) 97
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 3 cut(s) 168, 390, 564
BfuI GTATCC 1 cut(s) 97
BisI GCNGC 2 cut(s) 236, 301
BlsI GCNGC 2 cut(s) 237, 302
Bme18I GGWCC 1 cut(s) 401
BmeRI GACNNNNNGTC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 204
BmgT120I GGNCC 2 cut(s) 401, 535
BmiI GGNNCC 3 cut(s) 210, 255, 403
BmsI GCATC 3 cut(s) 469, 483, 491
BpmI CTGGAG 1 cut(s) 675
BsaHI GRCGYC 1 cut(s) 631
BsaWI WCCGGW 2 cut(s) 216, 250
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 310
Bse118I RCCGGY 2 cut(s) 250, 303
Bse1I ACTGG 1 cut(s) 548
BseGI GGATG 3 cut(s) 132, 136, 484
BseLI CCNNNNNNNGG 2 cut(s) 204, 310
BseNI ACTGG 1 cut(s) 548
BseRI GAGGAG 1 cut(s) 714
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 2 cut(s) 172, 537
BshTI ACCGGT 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 3 cut(s) 217, 251, 304
BslFI GGGAC 1 cut(s) 642
BslI CCNNNNNNNGG 2 cut(s) 204, 310
BsmAI GTCTC 1 cut(s) 180
BsmBI CGTCTC 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 642
BsnI GGCC 2 cut(s) 172, 537
BsoBI CYCGRG 1 cut(s) 204
Bsp143I GATC 4 cut(s) 49, 150, 226, 597
Bsp68I TCGCGA 1 cut(s) 59
BspACI CCGC 7 cut(s) 65, 91, 201, 233, 236, 301, 556
BspANI GGCC 2 cut(s) 172, 537
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 3 cut(s) 210, 255, 403
BspPI GGATC 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 203
BsrFI RCCGGY 2 cut(s) 250, 303
BsrI ACTGG 1 cut(s) 548
BssAI RCCGGY 2 cut(s) 250, 303
BssMI GATC 4 cut(s) 49, 150, 226, 597
BssNI GRCGYC 1 cut(s) 631
BssSI CACGAG 1 cut(s) 726
Bst2BI CACGAG 1 cut(s) 726
Bst4CI ACNGT 1 cut(s) 291
BstACI GRCGYC 1 cut(s) 631
BstC8I GCNNGC 1 cut(s) 609
BstF5I GGATG 3 cut(s) 132, 136, 484
BstFNI CGCG 1 cut(s) 59
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 4 cut(s) 52, 153, 229, 600
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 4 cut(s) 49, 150, 226, 597
BstMWI GCNNNNNNNGC 1 cut(s) 617
BstUI CGCG 1 cut(s) 59
BsuI GTATCC 1 cut(s) 97
BsuRI GGCC 2 cut(s) 172, 537
BtgZI GCGATG 2 cut(s) 74, 165
BtsCI GGATG 3 cut(s) 132, 136, 484
BtuMI TCGCGA 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 609
CfoI GCGC 1 cut(s) 331
Cfr10I RCCGGY 2 cut(s) 250, 303
Cfr13I GGNCC 2 cut(s) 401, 535
CseI GACGC 1 cut(s) 639
Csp6I GTAC 2 cut(s) 88, 705
CspAI ACCGGT 1 cut(s) 250
CviAII CATG 1 cut(s) 497
CviQI GTAC 2 cut(s) 88, 705
DpnI GATC 4 cut(s) 51, 152, 228, 599
DpnII GATC 4 cut(s) 49, 150, 226, 597
DriI GACNNNNNGTC 1 cut(s) 82
Eam1105I GACNNNNNGTC 1 cut(s) 82
EciI GGCGGA 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 204
EcoO109I RGGNCCY 1 cut(s) 535
EcoT22I ATGCAT 2 cut(s) 484, 498
Esp3I CGTCTC 1 cut(s) 180
FaeI CATG 1 cut(s) 500
FaiI YATR 9 cut(s) 6, 294, 351, 353, 441, 498, 518, 650, 701
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 1 cut(s) 642
FatI CATG 1 cut(s) 496
FauI CCCGC 1 cut(s) 58
Fnu4HI GCNGC 2 cut(s) 236, 301
FokI GGATG 3 cut(s) 139, 143, 491
Fsp4HI GCNGC 2 cut(s) 236, 301
FspBI CTAG 3 cut(s) 168, 390, 564
GlaI GCGC 1 cut(s) 330
GluI GCNGC 2 cut(s) 236, 301
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 2 cut(s) 172, 537
HapII CCGG 3 cut(s) 217, 251, 304
HgaI GACGC 1 cut(s) 639
HhaI GCGC 1 cut(s) 331
Hin1I GRCGYC 1 cut(s) 631
Hin1II CATG 1 cut(s) 500
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 6 cut(s) 23, 42, 77, 275, 396, 525
HpaII CCGG 3 cut(s) 217, 251, 304
HphI GGTGA 2 cut(s) 37, 593
Hpy166II GTNNAC 1 cut(s) 248
Hpy188I TCNGA 4 cut(s) 150, 213, 401, 415
Hpy188III TCNNGA 2 cut(s) 58, 522
Hpy8I GTNNAC 1 cut(s) 248
Hpy99I CGWCG 4 cut(s) 34, 190, 193, 196
HpyAV CCTTC 3 cut(s) 94, 586, 610
HpyCH4III ACNGT 1 cut(s) 291
HpyCH4V TGCA 4 cut(s) 439, 482, 496, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 617
Hsp92I GRCGYC 1 cut(s) 631
Hsp92II CATG 1 cut(s) 500
HspAI GCGC 1 cut(s) 329
Kzo9I GATC 4 cut(s) 49, 150, 226, 597
LpnPI CCDG 5 cut(s) 230, 264, 317, 561, 639
LweI GCATC 3 cut(s) 469, 483, 491
MaeI CTAG 3 cut(s) 168, 390, 564
MaeIII GTNAC 3 cut(s) 35, 43, 467
MalI GATC 4 cut(s) 51, 152, 228, 599
MbiI CCGCTC 1 cut(s) 203
MboI GATC 4 cut(s) 49, 150, 226, 597
MboII GAAGA 1 cut(s) 221
MfeI CAATTG 2 cut(s) 406, 639
MlyI GAGTC 4 cut(s) 32, 51, 71, 269
MnlI CCTC 9 cut(s) 9, 150, 167, 175, 208, 619, 650, 732, 735
Mph1103I ATGCAT 2 cut(s) 484, 498
MseI TTAA 1 cut(s) 341
MspI CCGG 3 cut(s) 217, 251, 304
MunI CAATTG 2 cut(s) 406, 639
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 617
NdeII GATC 4 cut(s) 49, 150, 226, 597
NlaIII CATG 1 cut(s) 500
NlaIV GGNNCC 3 cut(s) 210, 255, 403
NmuCI GTSAC 2 cut(s) 43, 467
NruI TCGCGA 1 cut(s) 59
NsiI ATGCAT 2 cut(s) 484, 498
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PfeI GAWTC 2 cut(s) 396, 525
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 2 cut(s) 237, 302
PleI GAGTC 4 cut(s) 31, 50, 71, 269
PpsI GAGTC 4 cut(s) 31, 50, 71, 269
PsiI TTATAA 1 cut(s) 701
PspN4I GGNNCC 3 cut(s) 210, 255, 403
PspPI GGNCC 2 cut(s) 401, 535
RruI TCGCGA 1 cut(s) 59
RsaI GTAC 2 cut(s) 89, 706
RsaNI GTAC 2 cut(s) 88, 705
SaqAI TTAA 1 cut(s) 341
SatI GCNGC 2 cut(s) 236, 301
Sau3AI GATC 4 cut(s) 49, 150, 226, 597
Sau96I GGNCC 2 cut(s) 401, 535
SchI GAGTC 4 cut(s) 32, 51, 71, 269
SetI ASST 4 cut(s) 20, 159, 462, 661
SfaNI GCATC 3 cut(s) 469, 483, 491
SgrAI CRCCGGYG 1 cut(s) 303
SinI GGWCC 1 cut(s) 401
SsiI CCGC 7 cut(s) 65, 91, 201, 233, 236, 301, 556
SspMI CTAG 3 cut(s) 168, 390, 564
TaaI ACNGT 1 cut(s) 291
TaqI TCGA 5 cut(s) 21, 153, 262, 270, 521
TaqII GACCGA 1 cut(s) 89
TauI GCSGC 2 cut(s) 238, 303
TfiI GAWTC 2 cut(s) 396, 525
Tru1I TTAA 1 cut(s) 341
Tru9I TTAA 1 cut(s) 341
TseFI GTSAC 2 cut(s) 43, 467
Tsp45I GTSAC 2 cut(s) 43, 467
TspDTI ATGAA 5 cut(s) 452, 470, 517, 587, 651
VpaK11BI GGWCC 1 cut(s) 401
XapI RAATTY 3 cut(s) 380, 426, 448
XspI CTAG 3 cut(s) 168, 390, 564
Zsp2I ATGCAT 2 cut(s) 484, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.