Rh5DG285400

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000387
Physical Location & Seq
Forward (+)
35304094 .. 35307426
3333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG285400.1

Sequence Viewer

Length: 903 bp
ATGCTTGGCGCTATAAGGCGAAGACTCGTCTCTGGAGCCTCCTCTCCTTCGACTTTGGGCCACTCATTGTCTTCAATCCGACCTATTGCAAACAAGGAGATTTTACCTCAAATCGGAGGAGGATTGGGTCATGTGCGCAATTATTCTCACCTCATTTTAGCTGGTTCATCGCATTGTATCAGAACTCCGAGGGATGTTGGAAGCTCTATTCAGCCAGAGCCTATCAGGCAAATGTGGAGCAGGCCCTTTTCTTCAGAGACTGGGGATTTGGTTGACGCCGTTGTGCCTTTTATGGGTGAATCTATTACTGATGGAACTTTGGCGACATTCTTGAAGAAGCCTGGAGACCGGGTGGAAGTTGATGAGCCGATTGCTCAAATAGAAACAGATAAGGTCACGATCGATGTTGCTAGTCCTGAATCTGGTGTAATTGAGAAGTTTGTAGCCAATGAAGGGGATACTGTGGAACCAGGCACCAAGATTGCTATCATACTAAAGTCCGGTGAAGGTGTATCCCATGTGGGTCCATCTGAGAAAACACTGGAGGAAGCTATTCCACAACCAGCTTCCCCTGTAGAAACAATCAGCAAGAATGAAACATCTAAAGTTGAAACAACTCCAGTTACCGATAAGCCTAAAGGACCCTCCCCACCATCTCCCAAACCCTCAGCTCAAGAACCTCAGCTCCCCCCTAAGGAACGAGAAAGACGATCGGCAATCTTGGGTATGCACTCAATAGTGACCCGGCCAATGGTTGTTGGGGGTGAGATTGTCCCGCGGCCGATGATGTACATTGCTCTTACCTATGACCATAGGCTGATTGATGGAAGAGAGGCAGTTTACTTCTTGCGTCGTGTTAAAGATGTTGTGGAGGACCCAAGGAGGCTTCTCCTTGATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

300

Amino Acids

32.49

Weight (kDa)

6.2

Isoelectric Point (pI)

60.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 95 - 164 1.8e-17 Biotin-requiring enzyme
BSH_YhbJ PF25997 103 - 162 1e-05 YhbJ, barrel-sandwich hybrid domain
BSH_CusB PF25919 111 - 164 8.4e-06 CusB, barrel-sandwich hybrid domain
2-oxoacid_dh PF00198 237 - 298 1.7e-18 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 473
AccII CGCG 1 cut(s) 778
AciI CCGC 2 cut(s) 776, 778
AcoI YGGCCR 2 cut(s) 746, 779
AcuI CTGAAG 1 cut(s) 237
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 1 cut(s) 791
AfiI CCNNNNNNNGG 6 cut(s) 113, 293, 422, 453, 694, 751
AgsI TTSAA 3 cut(s) 75, 334, 611
AjnI CCWGG 2 cut(s) 340, 469
AloI GAACNNNNNNTCC 2 cut(s) 669, 701
AluBI AGCT 6 cut(s) 161, 204, 551, 566, 671, 685
AluI AGCT 6 cut(s) 161, 204, 551, 566, 671, 685
Alw26I GTCTC 3 cut(s) 34, 251, 339
AlwNI CAGNNNCTG 1 cut(s) 260
AoxI GGCC 4 cut(s) 58, 242, 746, 779
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
Asp700I GAANNNNTTC 1 cut(s) 552
AspLEI GCGC 2 cut(s) 11, 138
AspS9I GGNCC 5 cut(s) 58, 243, 524, 641, 874
AsuC2I CCSGG 2 cut(s) 350, 745
AsuHPI GGTGA 4 cut(s) 140, 308, 515, 776
AvaII GGWCC 3 cut(s) 524, 641, 874
AxyI CCTNAGG 1 cut(s) 693
BanI GGYRCC 1 cut(s) 473
BbsI GAAGAC 2 cut(s) 28, 63
BbvCI CCTCAGC 2 cut(s) 667, 681
BccI CCATC 4 cut(s) 305, 535, 661, 818
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 2 cut(s) 342, 471
BciVI GTATCC 2 cut(s) 451, 523
BcnI CCSGG 2 cut(s) 350, 745
BcoDI GTCTC 3 cut(s) 34, 251, 339
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 573
BfoI RGCGCY 1 cut(s) 12
BfuI GTATCC 2 cut(s) 451, 523
BglI GCCNNNNNGGC 1 cut(s) 226
BisI GCNGC 1 cut(s) 779
BlsI GCNGC 1 cut(s) 780
Bme1390I CCNGG 4 cut(s) 342, 350, 471, 745
Bme18I GGWCC 3 cut(s) 524, 641, 874
BmgT120I GGNCC 5 cut(s) 58, 243, 524, 641, 874
BmiI GGNNCC 6 cut(s) 37, 468, 475, 525, 643, 876
BmrFI CCNGG 4 cut(s) 342, 350, 471, 745
BmrI ACTGGG 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 28, 63
BpmI CTGGAG 4 cut(s) 54, 363, 563, 603
Bpu10I CCTNAGC 2 cut(s) 667, 681
BpuEI CTTGAG 1 cut(s) 657
BpuMI CCSGG 2 cut(s) 350, 745
Bsa29I ATCGAT 1 cut(s) 402
BsaBI GATNNNNATC 1 cut(s) 485
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 188, 776, 878
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 669, 699
Bsc4I CCNNNNNNNGG 6 cut(s) 113, 293, 422, 453, 694, 751
Bse1I ACTGG 3 cut(s) 265, 546, 620
Bse21I CCTNAGG 1 cut(s) 693
Bse3DI GCAATG 1 cut(s) 792
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 2 cut(s) 342, 471
BseCI ATCGAT 1 cut(s) 402
BseDI CCNNGG 3 cut(s) 188, 776, 878
BseGI GGATG 1 cut(s) 199
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 6 cut(s) 113, 293, 422, 453, 694, 751
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 3 cut(s) 522, 681, 695
BseNI ACTGG 3 cut(s) 265, 546, 620
BseRI GAGGAG 2 cut(s) 31, 132
BseX3I CGGCCG 1 cut(s) 779
Bsh1236I CGCG 1 cut(s) 778
Bsh1285I CGRYCG 3 cut(s) 402, 713, 782
BshFI GGCC 4 cut(s) 60, 244, 748, 781
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 402
BsiEI CGRYCG 3 cut(s) 402, 713, 782
BsiSI CCGG 3 cut(s) 349, 501, 745
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 6 cut(s) 113, 293, 422, 453, 694, 751
BsmAI GTCTC 3 cut(s) 34, 251, 339
BsmBI CGTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 758
BsnI GGCC 4 cut(s) 60, 244, 748, 781
Bso31I GGTCTC 1 cut(s) 339
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 2 cut(s) 399, 710
BspACI CCGC 2 cut(s) 776, 778
BspANI GGCC 4 cut(s) 60, 244, 748, 781
BspCNI CTCAG 3 cut(s) 523, 680, 694
BspDI ATCGAT 1 cut(s) 402
BspFNI CGCG 1 cut(s) 778
BspLI GGNNCC 6 cut(s) 37, 468, 475, 525, 643, 876
BspT107I GGYRCC 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 792
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 3 cut(s) 265, 546, 620
BssECI CCNNGG 3 cut(s) 188, 776, 878
BssMI GATC 2 cut(s) 399, 710
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 878
Bst2UI CCWGG 2 cut(s) 342, 471
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 1 cut(s) 823
BstACI GRCGYC 1 cut(s) 276
BstAUI TGTACA 1 cut(s) 789
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 4 cut(s) 531, 667, 681, 693
BstDSI CCRYGG 1 cut(s) 776
BstF5I GGATG 1 cut(s) 199
BstFNI CGCG 1 cut(s) 778
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 138
BstKTI GATC 2 cut(s) 402, 713
BstMAI GTCTC 3 cut(s) 34, 251, 339
BstMBI GATC 2 cut(s) 399, 710
BstMCI CGRYCG 3 cut(s) 402, 713, 782
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 2 cut(s) 342, 471
BstSCI CCNGG 4 cut(s) 340, 348, 469, 743
BstSFI CTRYAG 1 cut(s) 573
BstUI CGCG 1 cut(s) 778
BstV2I GAAGAC 2 cut(s) 28, 63
BstZI CGGCCG 1 cut(s) 779
Bsu15I ATCGAT 1 cut(s) 402
Bsu36I CCTNAGG 1 cut(s) 693
BsuI GTATCC 2 cut(s) 451, 523
BsuRI GGCC 4 cut(s) 60, 244, 748, 781
BsuTUI ATCGAT 1 cut(s) 402
BtgI CCRYGG 1 cut(s) 776
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 539
Cac8I GCNNGC 1 cut(s) 242
CaiI CAGNNNCTG 1 cut(s) 260
CfoI GCGC 2 cut(s) 11, 138
Cfr13I GGNCC 5 cut(s) 58, 243, 524, 641, 874
Cfr42I CCGCGG 1 cut(s) 779
ClaI ATCGAT 1 cut(s) 402
CseI GACGC 2 cut(s) 284, 839
Csp6I GTAC 1 cut(s) 790
CviAII CATG 2 cut(s) 131, 518
CviQI GTAC 1 cut(s) 790
DdeI CTNAG 4 cut(s) 531, 667, 681, 693
DpnI GATC 2 cut(s) 401, 712
DpnII GATC 2 cut(s) 399, 710
EaeI YGGCCR 2 cut(s) 746, 779
EagI CGGCCG 1 cut(s) 779
Eam1104I CTCTTC 1 cut(s) 823
EarI CTCTTC 1 cut(s) 823
EclXI CGGCCG 1 cut(s) 779
Eco130I CCWWGG 1 cut(s) 878
Eco31I GGTCTC 1 cut(s) 339
Eco47I GGWCC 3 cut(s) 524, 641, 874
Eco52I CGGCCG 1 cut(s) 779
Eco57I CTGAAG 1 cut(s) 237
Eco81I CCTNAGG 1 cut(s) 693
EcoO109I RGGNCCY 3 cut(s) 243, 641, 874
EcoRII CCWGG 2 cut(s) 340, 469
EcoT14I CCWWGG 1 cut(s) 878
ErhI CCWWGG 1 cut(s) 878
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 2 cut(s) 134, 521
FaiI YATR 9 cut(s) 14, 132, 293, 491, 519, 728, 807, 813, 901
FaqI GGGAC 1 cut(s) 758
FatI CATG 2 cut(s) 130, 517
FauI CCCGC 1 cut(s) 783
Fnu4HI GCNGC 1 cut(s) 779
FokI GGATG 1 cut(s) 206
Fsp4HI GCNGC 1 cut(s) 779
FspBI CTAG 1 cut(s) 411
FspI TGCGCA 1 cut(s) 137
GlaI GCGC 2 cut(s) 10, 137
GluI GCNGC 1 cut(s) 779
GsuI CTGGAG 4 cut(s) 54, 363, 563, 603
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 4 cut(s) 60, 244, 748, 781
HapII CCGG 3 cut(s) 349, 501, 745
HgaI GACGC 2 cut(s) 284, 839
HhaI GCGC 2 cut(s) 11, 138
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 2 cut(s) 134, 521
Hin6I GCGC 2 cut(s) 9, 136
HinP1I GCGC 2 cut(s) 9, 136
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 3 cut(s) 24, 299, 419
HpaII CCGG 3 cut(s) 349, 501, 745
HphI GGTGA 4 cut(s) 140, 308, 515, 776
Hpy166II GTNNAC 2 cut(s) 274, 841
Hpy188I TCNGA 6 cut(s) 80, 116, 182, 189, 256, 532
Hpy188III TCNNGA 5 cut(s) 33, 331, 397, 416, 674
Hpy8I GTNNAC 2 cut(s) 274, 841
Hpy99I CGWCG 1 cut(s) 855
HpyAV CCTTC 3 cut(s) 57, 446, 500
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 2 cut(s) 89, 730
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 4 cut(s) 531, 667, 681, 693
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 2 cut(s) 134, 521
HspAI GCGC 2 cut(s) 9, 136
KspI CCGCGG 1 cut(s) 779
Kzo9I GATC 2 cut(s) 399, 710
LmnI GCTCC 3 cut(s) 35, 237, 690
MaeI CTAG 1 cut(s) 411
MaeIII GTNAC 3 cut(s) 394, 622, 739
MalI GATC 2 cut(s) 401, 712
MboI GATC 2 cut(s) 399, 710
MboII GAAGA 5 cut(s) 33, 63, 243, 346, 840
MluCI AATT 2 cut(s) 139, 429
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 2 cut(s) 103, 178
MroXI GAANNNNTTC 1 cut(s) 552
MseI TTAA 1 cut(s) 858
MspA1I CMGCKG 1 cut(s) 778
MspI CCGG 3 cut(s) 349, 501, 745
MspR9I CCNGG 4 cut(s) 342, 350, 471, 745
MvaI CCWGG 2 cut(s) 342, 471
MvnI CGCG 1 cut(s) 778
MwoI GCNNNNNNNGC 1 cut(s) 226
NciI CCSGG 2 cut(s) 350, 745
NdeII GATC 2 cut(s) 399, 710
NlaIII CATG 2 cut(s) 134, 521
NlaIV GGNNCC 6 cut(s) 37, 468, 475, 525, 643, 876
NmuCI GTSAC 2 cut(s) 394, 739
NsbI TGCGCA 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 706
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 2 cut(s) 299, 419
PkrI GCNGC 1 cut(s) 780
Ple19I CGATCG 2 cut(s) 402, 713
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
PpuMI RGGWCCY 2 cut(s) 641, 874
Psp5II RGGWCCY 2 cut(s) 641, 874
Psp6I CCWGG 2 cut(s) 340, 469
PspGI CCWGG 2 cut(s) 340, 469
PspN4I GGNNCC 6 cut(s) 37, 468, 475, 525, 643, 876
PspPI GGNCC 5 cut(s) 58, 243, 524, 641, 874
PspPPI RGGWCCY 2 cut(s) 641, 874
PstNI CAGNNNCTG 1 cut(s) 260
PvuI CGATCG 2 cut(s) 402, 713
RsaI GTAC 1 cut(s) 791
RsaNI GTAC 1 cut(s) 790
SacII CCGCGG 1 cut(s) 779
SaqAI TTAA 1 cut(s) 858
SatI GCNGC 1 cut(s) 779
Sau3AI GATC 2 cut(s) 399, 710
Sau96I GGNCC 5 cut(s) 58, 243, 524, 641, 874
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 4 cut(s) 342, 350, 471, 745
SfcI CTRYAG 1 cut(s) 573
Sfr303I CCGCGG 1 cut(s) 779
SgrBI CCGCGG 1 cut(s) 779
SinI GGWCC 3 cut(s) 524, 641, 874
SmlI CTYRAG 1 cut(s) 672
SmoI CTYRAG 1 cut(s) 672
Sse9I AATT 2 cut(s) 139, 429
SsiI CCGC 2 cut(s) 776, 778
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 4 cut(s) 340, 348, 469, 743
StyI CCWWGG 1 cut(s) 878
TaaI ACNGT 1 cut(s) 463
TaqI TCGA 2 cut(s) 50, 402
TasI AATT 2 cut(s) 139, 429
TatI WGTACW 1 cut(s) 789
TauI GCSGC 1 cut(s) 781
TfiI GAWTC 2 cut(s) 299, 419
Tru1I TTAA 1 cut(s) 858
Tru9I TTAA 1 cut(s) 858
TscAI CASTG 1 cut(s) 546
TseFI GTSAC 2 cut(s) 394, 739
Tsp45I GTSAC 2 cut(s) 394, 739
TspDTI ATGAA 3 cut(s) 156, 465, 609
TspRI CASTG 1 cut(s) 546
VpaK11BI GGWCC 3 cut(s) 524, 641, 874
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.