Rh5CG308700

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000387
Physical Location & Seq
Reverse (-)
34086827 .. 34091351
4525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG308700.1

Sequence Viewer

Length: 1209 bp
ATGCTTGGCGCTATAAGGCGAAGACTCGTCTCTGGAGCCTCCTCTCCTTCGATTTTGGGCCACTCATTGTCTTCAATCCGACCTATTGCAAACAAGGAGATTTTACCTCAAATCGGAGGAGGATTGGGTCATGTGCGCAATTATTCTCACCTCATTTTAGCAGGTTCATCTCATTGTATCAGAACTCCGAGGGATGTTGGAAGCTCTATTCAGCCAGAGCCTATCAGGCAAATGTGGAGCAGACCCTTTTCTTCTGAGACTGGGGATTTGGTTGACGCCGTTGTGCCTTTTATGGGTGAATCTATTACTGATGGAACTTTGGCGACATTCTTGAAGAAGCCTGGAGACCGGGTGGAAGTTGATGAACCAATTGCTCAAATTGAAACAGATAAGGTCACGATTGATGTTGCTAGTCCTGAATCCGGTGTAATTGAGAAGTTTGTAGCCAATGAAGGGGATACTGTGGAACCAGGCACCAAGATTGCTGTCATACTAAAGTCTGGTGAAGATGTATCCCATGTGGGTCCATCTGAGAAAACATTGGAGAAAGCTATTCCACAACCAGCTTCCCCTGTAGAAACAATTAGCAAGAATCAAACATCTAAAGTTGAAACAACTCTAGTTACTGAGAAGCCTAAAGCTCCCTCCCCACCATCTCCCAAACCCTCAGCTCAAGAACCTCAGCTCCCCCCTAAGGAAAGAGAAAGACGGATTCCTATGACAACGCTTCGGAAACGGATTGCTAAAAGATTGAAAGATTCACAGAACACATATGCAATGCTTACAACATTCAATGAAGTTGATATGACAAATTTGATGAAGCTTCGTTCTGACTTCAAGGATGCATTTGTTGAGAAGCATGGGGTCAAGTTGGGGCTTATGTCGGGATTCATCAAGGCTGCTGTTAGTGGGCTCCAAAATCAGCCTACTATTAATGCAGTCATTGATGGGGATGATATCATATATAGAGATTACATAGATATAAGTGTAGCCGTGGGTACTCCAAAGGGCCTTGTTGTGCCAGTTATTCGTAATGCTGATAAGTTGAATTGTGCTGAGATAGAGAAGGAGATCAATTTCCTCGCAAAGAAGGCAAATGAGGGACGTCTTTCAATTGATGAGATGGCTGGAGGTTCATTTACAATATCCAATGGTGGTGTTTATGGAAGCCTTATAAGTACCCCCATCATCAACCCTCCTCAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

402

Amino Acids

43.36

Weight (kDa)

7.04

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 95 - 164 2.8e-17 Biotin-requiring enzyme
BSH_CusB PF25919 111 - 164 9.1e-06 CusB, barrel-sandwich hybrid domain
2-oxoacid_dh PF00198 233 - 402 7.3e-58 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1175
AatII GACGTC 1 cut(s) 1108
Acc16I TGCGCA 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 152
AccB1I GGYRCC 1 cut(s) 473
AcsI RAATTY 1 cut(s) 811
AcyI GRCGYC 2 cut(s) 276, 1105
AfaI GTAC 2 cut(s) 1000, 1180
AfiI CCNNNNNNNGG 6 cut(s) 113, 293, 422, 453, 694, 1201
AgsI TTSAA 9 cut(s) 75, 334, 383, 611, 754, 793, 838, 1048, 1113
AjnI CCWGG 2 cut(s) 340, 469
AloI GAACNNNNNNTCC 2 cut(s) 669, 701
AluBI AGCT 7 cut(s) 204, 551, 566, 641, 671, 685, 823
AluI AGCT 7 cut(s) 204, 551, 566, 641, 671, 685, 823
Alw26I GTCTC 3 cut(s) 34, 251, 339
AoxI GGCC 2 cut(s) 58, 1009
ApeKI GCWGC 1 cut(s) 899
ApoI RAATTY 1 cut(s) 811
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
AseI ATTAAT 1 cut(s) 933
AspLEI GCGC 2 cut(s) 11, 138
AspS9I GGNCC 3 cut(s) 58, 524, 1009
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 3 cut(s) 140, 308, 515
AvaII GGWCC 1 cut(s) 524
AxyI CCTNAGG 2 cut(s) 693, 1200
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 1 cut(s) 915
BbsI GAAGAC 2 cut(s) 28, 63
BbvCI CCTCAGC 2 cut(s) 667, 681
BbvI GCAGC 1 cut(s) 886
BccI CCATC 6 cut(s) 305, 535, 661, 941, 1117, 1193
BceAI ACGGC 2 cut(s) 263, 977
BciT130I CCWGG 2 cut(s) 342, 471
BciVI GTATCC 2 cut(s) 451, 523
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 3 cut(s) 34, 251, 339
BfaI CTAG 2 cut(s) 411, 620
BfmI CTRYAG 1 cut(s) 573
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 152
BfuI GTATCC 2 cut(s) 451, 523
BglI GCCNNNNNGGC 1 cut(s) 226
BisI GCNGC 1 cut(s) 900
BlsI GCNGC 1 cut(s) 901
Bme1390I CCNGG 3 cut(s) 342, 350, 471
Bme18I GGWCC 1 cut(s) 524
BmgT120I GGNCC 3 cut(s) 58, 524, 1009
BmiI GGNNCC 5 cut(s) 37, 468, 475, 525, 914
BmrFI CCNGG 3 cut(s) 342, 350, 471
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 832
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 28, 63
BpmI CTGGAG 3 cut(s) 54, 363, 1149
Bpu10I CCTNAGC 2 cut(s) 667, 681
BpuEI CTTGAG 1 cut(s) 657
BpuMI CCSGG 1 cut(s) 350
BsaHI GRCGYC 2 cut(s) 276, 1105
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 188, 993
BsaWI WCCGGW 1 cut(s) 422
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 669, 699
Bsc4I CCNNNNNNNGG 6 cut(s) 113, 293, 422, 453, 694, 1201
Bse1I ACTGG 2 cut(s) 265, 1022
Bse21I CCTNAGG 2 cut(s) 693, 1200
Bse3DI GCAATG 1 cut(s) 783
BseBI CCWGG 2 cut(s) 342, 471
BseDI CCNNGG 2 cut(s) 188, 993
BseGI GGATG 3 cut(s) 199, 847, 958
BseLI CCNNNNNNNGG 6 cut(s) 113, 293, 422, 453, 694, 1201
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 6 cut(s) 246, 522, 618, 681, 695, 1047
BseNI ACTGG 2 cut(s) 265, 1022
BseRI GAGGAG 3 cut(s) 31, 132, 1188
BseXI GCAGC 1 cut(s) 886
BshFI GGCC 2 cut(s) 60, 1011
BshNI GGYRCC 1 cut(s) 473
BsiSI CCGG 2 cut(s) 349, 423
BslFI GGGAC 1 cut(s) 1116
BslI CCNNNNNNNGG 6 cut(s) 113, 293, 422, 453, 694, 1201
BsmAI GTCTC 3 cut(s) 34, 251, 339
BsmBI CGTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 1116
BsnI GGCC 2 cut(s) 60, 1011
Bso31I GGTCTC 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 915
Bsp143I GATC 1 cut(s) 1071
BspANI GGCC 2 cut(s) 60, 1011
BspCNI CTCAG 6 cut(s) 247, 523, 619, 680, 694, 1048
BspLI GGNNCC 5 cut(s) 37, 468, 475, 525, 914
BspMI ACCTGC 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 783
BsrI ACTGG 2 cut(s) 265, 1022
BssECI CCNNGG 2 cut(s) 188, 993
BssMI GATC 1 cut(s) 1071
BssNI GRCGYC 2 cut(s) 276, 1105
Bst2UI CCWGG 2 cut(s) 342, 471
Bst4CI ACNGT 1 cut(s) 463
BstACI GRCGYC 2 cut(s) 276, 1105
BstDEI CTNAG 8 cut(s) 255, 531, 627, 667, 681, 693, 1056, 1200
BstDSI CCRYGG 1 cut(s) 993
BstF5I GGATG 3 cut(s) 199, 847, 958
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 138
BstKTI GATC 1 cut(s) 1074
BstMAI GTCTC 3 cut(s) 34, 251, 339
BstMBI GATC 1 cut(s) 1071
BstMWI GCNNNNNNNGC 2 cut(s) 226, 1091
BstNI CCWGG 2 cut(s) 342, 471
BstSCI CCNGG 3 cut(s) 340, 348, 469
BstSFI CTRYAG 1 cut(s) 573
BstV1I GCAGC 1 cut(s) 886
BstV2I GAAGAC 2 cut(s) 28, 63
Bsu36I CCTNAGG 2 cut(s) 693, 1200
BsuI GTATCC 2 cut(s) 451, 523
BsuRI GGCC 2 cut(s) 60, 1011
BtgI CCRYGG 1 cut(s) 993
BtsCI GGATG 3 cut(s) 199, 847, 958
BveI ACCTGC 1 cut(s) 152
CfoI GCGC 2 cut(s) 11, 138
Cfr13I GGNCC 3 cut(s) 58, 524, 1009
CseI GACGC 1 cut(s) 284
Csp6I GTAC 2 cut(s) 999, 1179
CviAII CATG 3 cut(s) 131, 518, 860
CviQI GTAC 2 cut(s) 999, 1179
DdeI CTNAG 8 cut(s) 255, 531, 627, 667, 681, 693, 1056, 1200
DpnI GATC 1 cut(s) 1073
DpnII GATC 1 cut(s) 1071
Eco24I GRGCYC 1 cut(s) 915
Eco31I GGTCTC 1 cut(s) 339
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 1 cut(s) 524
Eco81I CCTNAGG 2 cut(s) 693, 1200
EcoO109I RGGNCCY 1 cut(s) 1009
EcoRII CCWGG 2 cut(s) 340, 469
EcoRV GATATC 1 cut(s) 958
EcoT22I ATGCAT 1 cut(s) 847
EcoT38I GRGCYC 1 cut(s) 915
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 3 cut(s) 134, 521, 863
FaqI GGGAC 1 cut(s) 1116
FatI CATG 3 cut(s) 130, 517, 859
FauNDI CATATG 1 cut(s) 772
Fnu4HI GCNGC 1 cut(s) 900
FokI GGATG 3 cut(s) 206, 854, 965
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 1 cut(s) 900
FspBI CTAG 2 cut(s) 411, 620
FspI TGCGCA 1 cut(s) 137
GlaI GCGC 2 cut(s) 10, 137
GluI GCNGC 1 cut(s) 900
GsuI CTGGAG 3 cut(s) 54, 363, 1149
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 60, 1011
HapII CCGG 2 cut(s) 349, 423
HgaI GACGC 1 cut(s) 284
HhaI GCGC 2 cut(s) 11, 138
Hin1I GRCGYC 2 cut(s) 276, 1105
Hin1II CATG 3 cut(s) 134, 521, 863
Hin6I GCGC 2 cut(s) 9, 136
HinP1I GCGC 2 cut(s) 9, 136
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 821
HinfI GANTC 7 cut(s) 24, 299, 419, 592, 712, 758, 888
HpaII CCGG 2 cut(s) 349, 423
HphI GGTGA 3 cut(s) 140, 308, 515
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 9 cut(s) 80, 116, 182, 189, 256, 532, 732, 832, 1208
Hpy188III TCNNGA 6 cut(s) 33, 331, 397, 416, 674, 885
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 4 cut(s) 57, 446, 1060, 1084
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4IV ACGT 1 cut(s) 1105
HpyCH4V TGCA 4 cut(s) 89, 776, 845, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 1091
HpyF3I CTNAG 8 cut(s) 255, 531, 627, 667, 681, 693, 1056, 1200
HpySE526I ACGT 1 cut(s) 1105
Hsp92I GRCGYC 2 cut(s) 276, 1105
Hsp92II CATG 3 cut(s) 134, 521, 863
HspAI GCGC 2 cut(s) 9, 136
Kzo9I GATC 1 cut(s) 1071
LmnI GCTCC 5 cut(s) 35, 237, 646, 690, 918
Lsp1109I GCAGC 1 cut(s) 886
LweI GCATC 1 cut(s) 832
MaeI CTAG 2 cut(s) 411, 620
MaeII ACGT 1 cut(s) 1105
MaeIII GTNAC 2 cut(s) 394, 622
MalI GATC 1 cut(s) 1073
MboI GATC 1 cut(s) 1071
MboII GAAGA 5 cut(s) 33, 63, 243, 346, 518
MfeI CAATTG 2 cut(s) 369, 1113
MhlI GDGCHC 1 cut(s) 915
MluCI AATT 9 cut(s) 139, 369, 378, 429, 582, 811, 1048, 1075, 1113
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 2 cut(s) 103, 178
Mph1103I ATGCAT 1 cut(s) 847
MseI TTAA 1 cut(s) 933
MspI CCGG 2 cut(s) 349, 423
MspR9I CCNGG 3 cut(s) 342, 350, 471
MunI CAATTG 2 cut(s) 369, 1113
MvaI CCWGG 2 cut(s) 342, 471
MwoI GCNNNNNNNGC 2 cut(s) 226, 1091
NciI CCSGG 1 cut(s) 350
NdeI CATATG 1 cut(s) 772
NdeII GATC 1 cut(s) 1071
NlaIII CATG 3 cut(s) 134, 521, 863
NlaIV GGNNCC 5 cut(s) 37, 468, 475, 525, 914
NmuCI GTSAC 1 cut(s) 394
NsbI TGCGCA 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 847
PfeI GAWTC 6 cut(s) 299, 419, 592, 712, 758, 888
PkrI GCNGC 1 cut(s) 901
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
PshBI ATTAAT 1 cut(s) 933
PsiI TTATAA 1 cut(s) 1175
Psp6I CCWGG 2 cut(s) 340, 469
PspGI CCWGG 2 cut(s) 340, 469
PspN4I GGNNCC 5 cut(s) 37, 468, 475, 525, 914
PspPI GGNCC 3 cut(s) 58, 524, 1009
RsaI GTAC 2 cut(s) 1000, 1180
RsaNI GTAC 2 cut(s) 999, 1179
SaqAI TTAA 1 cut(s) 933
SatI GCNGC 1 cut(s) 900
Sau3AI GATC 1 cut(s) 1071
Sau96I GGNCC 3 cut(s) 58, 524, 1009
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 3 cut(s) 342, 350, 471
SduI GDGCHC 1 cut(s) 915
SfaNI GCATC 1 cut(s) 832
SfcI CTRYAG 1 cut(s) 573
SinI GGWCC 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 672
SmoI CTYRAG 1 cut(s) 672
Sse9I AATT 9 cut(s) 139, 369, 378, 429, 582, 811, 1048, 1075, 1113
SspMI CTAG 2 cut(s) 411, 620
StyD4I CCNGG 3 cut(s) 340, 348, 469
TaaI ACNGT 1 cut(s) 463
TaiI ACGT 1 cut(s) 1108
TaqI TCGA 1 cut(s) 50
TasI AATT 9 cut(s) 139, 369, 378, 429, 582, 811, 1048, 1075, 1113
TfiI GAWTC 6 cut(s) 299, 419, 592, 712, 758, 888
Tru1I TTAA 1 cut(s) 933
Tru9I TTAA 1 cut(s) 933
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 1 cut(s) 899
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 7 cut(s) 156, 378, 465, 810, 833, 880, 1125
TspGWI ACGGA 2 cut(s) 724, 751
VpaK11BI GGWCC 1 cut(s) 524
VspI ATTAAT 1 cut(s) 933
XapI RAATTY 1 cut(s) 811
XspI CTAG 2 cut(s) 411, 620
ZraI GACGTC 1 cut(s) 1106
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.