RchiOBHm_Chr2g0119431

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000387
Physical Location & Seq
Reverse (-)
31795854 .. 31796947
1094 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49214

Sequence Viewer

Length: 450 bp
ATGCCTATGACAATGCTTCGGAAATGGATTGCTAAAAGATTGAAAGATTCACAAAATACATATGCAATGCTTACAACATTCAATGAAGTTGATATGTTACATGGGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGGCTGCTGTCAGTGGGCTCCAAAATCAGCCTACTATTAATGAAGTCATCGATGGGGATGATATCATATATAGAGATTACATAGATATAAGTGTAGTTGTGGGTACTCCAAAGGGCCTTGTTGTGCCAGTTATCCGCAATGGTGATAAGATGAATTGTGATGAGATAGAGAAGGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACGGCTTTCAATTGATGATATGGCTAGAGGTTCATTTACAATATCCAATGGTGGATTTTATGGGAGCCTTATAAGTACCCTCATCATCAACCCTCCTCAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

149

Amino Acids

16.51

Weight (kDa)

6.57

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 2 - 149 1.2e-38 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 416
AciI CCGC 1 cut(s) 271
AfaI GTAC 2 cut(s) 241, 421
AfiI CCNNNNNNNGG 1 cut(s) 442
AgsI TTSAA 3 cut(s) 43, 82, 354
AoxI GGCC 1 cut(s) 250
ApeKI GCWGC 1 cut(s) 140
AseI ATTAAT 1 cut(s) 174
AspS9I GGNCC 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 290
AxyI CCTNAGG 1 cut(s) 441
BanII GRGCYC 1 cut(s) 156
BbvI GCAGC 1 cut(s) 127
BccI CCATC 1 cut(s) 182
BceAI ACGGC 1 cut(s) 362
BfaI CTAG 1 cut(s) 369
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 2 cut(s) 155, 410
Bsa29I ATCGAT 1 cut(s) 186
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse1I ACTGG 1 cut(s) 263
Bse21I CCTNAGG 1 cut(s) 441
Bse3DI GCAATG 2 cut(s) 72, 280
BseCI ATCGAT 1 cut(s) 186
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMI GCAATG 2 cut(s) 72, 280
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 1 cut(s) 429
BseXI GCAGC 1 cut(s) 127
BshFI GGCC 1 cut(s) 252
BshVI ATCGAT 1 cut(s) 186
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 442
BsmFI GGGAC 1 cut(s) 357
BsnI GGCC 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 1 cut(s) 312
BspACI CCGC 1 cut(s) 271
BspANI GGCC 1 cut(s) 252
BspDI ATCGAT 1 cut(s) 186
BspLI GGNNCC 2 cut(s) 155, 410
BsrDI GCAATG 2 cut(s) 72, 280
BsrI ACTGG 1 cut(s) 263
BssMI GATC 1 cut(s) 312
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 1 cut(s) 441
BstF5I GGATG 1 cut(s) 199
BstKTI GATC 1 cut(s) 315
BstMBI GATC 1 cut(s) 312
BstMWI GCNNNNNNNGC 1 cut(s) 332
BstV1I GCAGC 1 cut(s) 127
Bsu15I ATCGAT 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 441
BsuRI GGCC 1 cut(s) 252
BsuTUI ATCGAT 1 cut(s) 186
BtsCI GGATG 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 250
ClaI ATCGAT 1 cut(s) 186
Csp6I GTAC 2 cut(s) 240, 420
CviAII CATG 1 cut(s) 101
CviJI RGCY 8 cut(s) 118, 140, 154, 166, 252, 349, 368, 411
CviKI_1 RGCY 8 cut(s) 118, 140, 154, 166, 252, 349, 368, 411
CviQI GTAC 2 cut(s) 240, 420
DdeI CTNAG 1 cut(s) 441
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
Eco24I GRGCYC 1 cut(s) 156
Eco32I GATATC 1 cut(s) 199
Eco81I CCTNAGG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 250
EcoRV GATATC 1 cut(s) 199
EcoT38I GRGCYC 1 cut(s) 156
FaeI CATG 1 cut(s) 104
FaqI GGGAC 1 cut(s) 357
FatI CATG 1 cut(s) 100
FauNDI CATATG 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 369
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 1 cut(s) 252
Hin1II CATG 1 cut(s) 104
HinfI GANTC 2 cut(s) 47, 129
HphI GGTGA 1 cut(s) 290
Hpy188I TCNGA 2 cut(s) 21, 449
Hpy188III TCNNGA 1 cut(s) 126
HpyAV CCTTC 2 cut(s) 301, 325
HpyCH4V TGCA 2 cut(s) 65, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 332
HpyF3I CTNAG 1 cut(s) 441
Hsp92II CATG 1 cut(s) 104
Kzo9I GATC 1 cut(s) 312
LmnI GCTCC 2 cut(s) 159, 408
LpnPI CCDG 2 cut(s) 276, 428
Lsp1109I GCAGC 1 cut(s) 127
MaeI CTAG 1 cut(s) 369
MaeIII GTNAC 1 cut(s) 96
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MfeI CAATTG 1 cut(s) 354
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 3 cut(s) 289, 316, 354
MnlI CCTC 5 cut(s) 334, 365, 434, 447, 450
MseI TTAA 1 cut(s) 174
MunI CAATTG 1 cut(s) 354
MwoI GCNNNNNNNGC 1 cut(s) 332
NdeI CATATG 1 cut(s) 61
NdeII GATC 1 cut(s) 312
NlaIII CATG 1 cut(s) 104
NlaIV GGNNCC 2 cut(s) 155, 410
PfeI GAWTC 2 cut(s) 47, 129
PkrI GCNGC 1 cut(s) 142
PshBI ATTAAT 1 cut(s) 174
PsiI TTATAA 1 cut(s) 416
PspN4I GGNNCC 2 cut(s) 155, 410
PspPI GGNCC 1 cut(s) 250
RsaI GTAC 2 cut(s) 241, 421
RsaNI GTAC 2 cut(s) 240, 420
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 1 cut(s) 312
Sau96I GGNCC 1 cut(s) 250
SduI GDGCHC 1 cut(s) 156
SetI ASST 2 cut(s) 376, 447
SgeI CNNG 8 cut(s) 113, 121, 138, 148, 266, 275, 335, 381
Sse9I AATT 3 cut(s) 289, 316, 354
SsiI CCGC 1 cut(s) 271
SspMI CTAG 1 cut(s) 369
TaqI TCGA 2 cut(s) 125, 186
TasI AATT 3 cut(s) 289, 316, 354
TfiI GAWTC 2 cut(s) 47, 129
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 154
TseI GCWGC 1 cut(s) 140
TspDTI ATGAA 5 cut(s) 99, 121, 192, 302, 366
TspRI CASTG 1 cut(s) 154
VspI ATTAAT 1 cut(s) 174
XspI CTAG 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.