Rroxscaffold_5G00345830

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000387
Physical Location & Seq
Reverse (-)
16389025 .. 16389681
657 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00345830.1

Sequence Viewer

Length: 276 bp
ATGAAGCTTCGTTGTGACTTCAAGGATGCATATGTTGAGAAGCATGGGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGCCTACTATTAATGCAGTCATTGATGGGGATGATATCATACATAGAGATTATATGGATATAAGTGCAGCTGTGGGTACTCCAAAGGGCCTTGTTGTGCCGGATATCTGCAATGCTGATAAGATGAATTGTGATGAGATTGAGAAGGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGGACGTCTTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

91

Amino Acids

10.09

Weight (kDa)

5.59

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 28 - 88 9.7e-16 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 1 cut(s) 157
AgsI TTSAA 2 cut(s) 22, 271
AluBI AGCT 2 cut(s) 7, 149
AluI AGCT 2 cut(s) 7, 149
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 1 cut(s) 146
AseI ATTAAT 1 cut(s) 90
AspS9I GGNCC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 158
BccI CCATC 1 cut(s) 98
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 166
BmsI GCATC 1 cut(s) 16
BsaHI GRCGYC 1 cut(s) 263
Bse3DI GCAATG 1 cut(s) 196
BseGI GGATG 2 cut(s) 31, 115
BseMI GCAATG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 158
BsgI GTGCAG 1 cut(s) 165
BshFI GGCC 1 cut(s) 168
BsiSI CCGG 1 cut(s) 179
BsnI GGCC 1 cut(s) 168
Bsp143I GATC 1 cut(s) 228
BspANI GGCC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 196
BssMI GATC 1 cut(s) 228
BssNI GRCGYC 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 240
BstF5I GGATG 2 cut(s) 31, 115
BstKTI GATC 1 cut(s) 231
BstMBI GATC 1 cut(s) 228
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 158
BsuRI GGCC 1 cut(s) 168
BtsCI GGATG 2 cut(s) 31, 115
Cac8I GCNNGC 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 156
CviAII CATG 1 cut(s) 44
CviJI RGCY 5 cut(s) 7, 61, 82, 149, 168
CviKI_1 RGCY 5 cut(s) 7, 61, 82, 149, 168
CviQI GTAC 1 cut(s) 156
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
Eco32I GATATC 2 cut(s) 115, 184
EcoO109I RGGNCCY 1 cut(s) 166
EcoRV GATATC 2 cut(s) 115, 184
EcoT22I ATGCAT 1 cut(s) 31
FaeI CATG 1 cut(s) 47
FaiI YATR 9 cut(s) 31, 33, 45, 65, 119, 123, 132, 134, 140
FatI CATG 1 cut(s) 43
FauNDI CATATG 1 cut(s) 31
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 2 cut(s) 38, 122
Fsp4HI GCNGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 147
HaeIII GGCC 1 cut(s) 168
HapII CCGG 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 1 cut(s) 47
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 1 cut(s) 72
HpaII CCGG 1 cut(s) 179
Hpy188III TCNNGA 1 cut(s) 69
HpyAV CCTTC 2 cut(s) 217, 241
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 5 cut(s) 29, 95, 146, 189, 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpySE526I ACGT 1 cut(s) 263
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 1 cut(s) 47
Kzo9I GATC 1 cut(s) 228
LpnPI CCDG 1 cut(s) 192
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 1 cut(s) 16
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 1 cut(s) 14
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MfeI CAATTG 1 cut(s) 271
MluCI AATT 3 cut(s) 205, 232, 271
MnlI CCTC 1 cut(s) 250
Mph1103I ATGCAT 1 cut(s) 31
MseI TTAA 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 1 cut(s) 179
MunI CAATTG 1 cut(s) 271
MwoI GCNNNNNNNGC 1 cut(s) 248
NdeI CATATG 1 cut(s) 31
NdeII GATC 1 cut(s) 228
NlaIII CATG 1 cut(s) 47
NmuCI GTSAC 1 cut(s) 14
NsiI ATGCAT 1 cut(s) 31
PfeI GAWTC 1 cut(s) 72
PkrI GCNGC 1 cut(s) 148
PshBI ATTAAT 1 cut(s) 90
PspPI GGNCC 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 1 cut(s) 157
RsaNI GTAC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 1 cut(s) 228
Sau96I GGNCC 1 cut(s) 166
SetI ASST 3 cut(s) 9, 151, 266
SfaNI GCATC 1 cut(s) 16
SgeI CNNG 8 cut(s) 34, 56, 64, 81, 91, 182, 191, 251
Sse9I AATT 3 cut(s) 205, 232, 271
TaiI ACGT 1 cut(s) 266
TaqI TCGA 1 cut(s) 68
TasI AATT 3 cut(s) 205, 232, 271
TfiI GAWTC 1 cut(s) 72
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 3 cut(s) 17, 64, 218
VspI ATTAAT 1 cut(s) 90
ZraI GACGTC 1 cut(s) 264
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.