Rh6BG220000

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000387
Physical Location & Seq
Reverse (-)
41457682 .. 41458290
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG220000.1

Sequence Viewer

Length: 306 bp
ATGTTGAGATTCATCAAGGCTGCTGTCAATATAAGAGTAGCTGTGGGTACTCCAAAGGGCCTTGCTGTGCCAGTTATCCGCAATGCTGATAAGATGAATTGTGGTGAGATAGAGGAGGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACGTCTTTCAATTGATGATATGGCTAGAGAAGAGGGTGGATTTTATGGAAGCCTTATAAATACCCCATCATCAACCCACCTCAGGTCTGATTCTTTATCTGACCCAATTGACCGGACTTTGATCGTCTTACTGCAACAAATGGAACTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

101

Amino Acids

11.06

Weight (kDa)

5.34

Isoelectric Point (pI)

55.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 6 - 59 1.6e-15 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 209
AatII GACGTC 1 cut(s) 157
AciI CCGC 1 cut(s) 79
AcyI GRCGYC 1 cut(s) 154
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 234
AgsI TTSAA 1 cut(s) 162
AluBI AGCT 1 cut(s) 41
AluI AGCT 1 cut(s) 41
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 58
AsuHPI GGTGA 1 cut(s) 116
AxyI CCTNAGG 1 cut(s) 233
BbvI GCAGC 1 cut(s) 7
BccI CCATC 1 cut(s) 226
BfaI CTAG 1 cut(s) 177
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 154
BsaWI WCCGGW 1 cut(s) 264
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse1I ACTGG 1 cut(s) 71
Bse21I CCTNAGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 247
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 2 cut(s) 128, 131
BseXI GCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 60
BsiSI CCGG 1 cut(s) 265
BslFI GGGAC 1 cut(s) 165
BslI CCNNNNNNNGG 1 cut(s) 234
BsmFI GGGAC 1 cut(s) 165
BsnI GGCC 1 cut(s) 60
Bsp143I GATC 2 cut(s) 120, 273
BspACI CCGC 1 cut(s) 79
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 88
BsrI ACTGG 1 cut(s) 71
BssMI GATC 2 cut(s) 120, 273
BssNI GRCGYC 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 177
BstACI GRCGYC 1 cut(s) 154
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 1 cut(s) 233
BstKTI GATC 2 cut(s) 123, 276
BstMBI GATC 2 cut(s) 120, 273
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstV1I GCAGC 1 cut(s) 7
Bsu36I CCTNAGG 1 cut(s) 233
BsuRI GGCC 1 cut(s) 60
Cac8I GCNNGC 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 48
CviJI RGCY 5 cut(s) 20, 41, 60, 176, 204
CviKI_1 RGCY 5 cut(s) 20, 41, 60, 176, 204
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 233
DpnI GATC 2 cut(s) 122, 275
DpnII GATC 2 cut(s) 120, 273
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco81I CCTNAGG 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 58
FaiI YATR 5 cut(s) 32, 173, 198, 209, 304
FaqI GGGAC 1 cut(s) 165
Fnu4HI GCNGC 1 cut(s) 21
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 1 cut(s) 177
GluI GCNGC 1 cut(s) 21
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 265
Hin1I GRCGYC 1 cut(s) 154
HinfI GANTC 2 cut(s) 9, 242
HpaII CCGG 1 cut(s) 265
HphI GGTGA 1 cut(s) 116
Hpy188I TCNGA 2 cut(s) 241, 253
HpyAV CCTTC 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 134, 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 233
HpySE526I ACGT 1 cut(s) 154
Hsp92I GRCGYC 1 cut(s) 154
Kzo9I GATC 2 cut(s) 120, 273
LpnPI CCDG 3 cut(s) 84, 220, 278
Lsp1109I GCAGC 1 cut(s) 7
MaeI CTAG 1 cut(s) 177
MaeII ACGT 1 cut(s) 154
MalI GATC 2 cut(s) 122, 275
MboI GATC 2 cut(s) 120, 273
MboII GAAGA 1 cut(s) 194
MfeI CAATTG 2 cut(s) 162, 258
MluCI AATT 4 cut(s) 97, 124, 162, 258
MnlI CCTC 5 cut(s) 106, 109, 142, 178, 242
MspI CCGG 1 cut(s) 265
MunI CAATTG 2 cut(s) 162, 258
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 2 cut(s) 120, 273
PfeI GAWTC 2 cut(s) 9, 242
PkrI GCNGC 1 cut(s) 22
PsiI TTATAA 1 cut(s) 209
PspPI GGNCC 1 cut(s) 58
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 2 cut(s) 120, 273
Sau96I GGNCC 1 cut(s) 58
SetI ASST 4 cut(s) 43, 157, 234, 239
SgeI CNNG 7 cut(s) 28, 74, 83, 143, 189, 247, 277
Sse9I AATT 4 cut(s) 97, 124, 162, 258
SsiI CCGC 1 cut(s) 79
SspMI CTAG 1 cut(s) 177
TaiI ACGT 1 cut(s) 157
TasI AATT 4 cut(s) 97, 124, 162, 258
TfiI GAWTC 2 cut(s) 9, 242
TseI GCWGC 1 cut(s) 20
TspDTI ATGAA 1 cut(s) 110
XspI CTAG 1 cut(s) 177
ZraI GACGTC 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.