Rroxscaffold_2G00127540

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000387
Physical Location & Seq
Forward (+)
63086621 .. 63090916
4296 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00127540.1

Sequence Viewer

Length: 165 bp
ATGAATTGTGGTGAGATAGAGAAGGAGATCAATTTGCTTGCAAAGAAGGCAAATGAAGGACGTCTTTCAATTGATGATATGGCTGGAGGTTCATTTACAATATCCAGTGGTGGATTTTATGGAAGCCTTATAAGTACCCCTATCATCAACCCTCCTCAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

54

Amino Acids

5.69

Weight (kDa)

4.66

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 1 - 54 5.3e-15 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AatII GACGTC 1 cut(s) 64
AcyI GRCGYC 1 cut(s) 61
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 1 cut(s) 157
AgsI TTSAA 1 cut(s) 69
AsuHPI GGTGA 1 cut(s) 23
AxyI CCTNAGG 1 cut(s) 156
BpmI CTGGAG 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 61
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 105
Bse21I CCTNAGG 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 157
BseNI ACTGG 1 cut(s) 105
BseRI GAGGAG 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 157
Bsp143I GATC 1 cut(s) 27
BsrI ACTGG 1 cut(s) 105
BssMI GATC 1 cut(s) 27
BssNI GRCGYC 1 cut(s) 61
BstACI GRCGYC 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 1 cut(s) 156
BstKTI GATC 1 cut(s) 30
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 1 cut(s) 47
Bsu36I CCTNAGG 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 39
Csp6I GTAC 1 cut(s) 135
CviJI RGCY 2 cut(s) 83, 126
CviKI_1 RGCY 2 cut(s) 83, 126
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 1 cut(s) 156
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
Eco81I CCTNAGG 1 cut(s) 156
FaiI YATR 3 cut(s) 80, 120, 131
FalI AAGNNNNNCTT 2 cut(s) 48, 80
GsuI CTGGAG 1 cut(s) 105
Hin1I GRCGYC 1 cut(s) 61
HphI GGTGA 1 cut(s) 23
Hpy188I TCNGA 1 cut(s) 164
HpyAV CCTTC 3 cut(s) 16, 40, 50
HpyCH4IV ACGT 1 cut(s) 61
HpyCH4V TGCA 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 1 cut(s) 61
Hsp92I GRCGYC 1 cut(s) 61
Kzo9I GATC 1 cut(s) 27
LpnPI CCDG 3 cut(s) 69, 118, 143
MaeII ACGT 1 cut(s) 61
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MfeI CAATTG 1 cut(s) 69
MluCI AATT 3 cut(s) 4, 31, 69
MnlI CCTC 3 cut(s) 80, 162, 165
MunI CAATTG 1 cut(s) 69
MwoI GCNNNNNNNGC 1 cut(s) 47
NdeII GATC 1 cut(s) 27
PsiI TTATAA 1 cut(s) 131
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
Sau3AI GATC 1 cut(s) 27
SetI ASST 3 cut(s) 64, 91, 162
SgeI CNNG 3 cut(s) 50, 96, 117
Sse9I AATT 3 cut(s) 4, 31, 69
TaiI ACGT 1 cut(s) 64
TasI AATT 3 cut(s) 4, 31, 69
TscAI CASTG 1 cut(s) 112
TspDTI ATGAA 3 cut(s) 17, 69, 81
TspRI CASTG 1 cut(s) 112
ZraI GACGTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.