Rh1AG275400

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000387
Physical Location & Seq
Reverse (-)
50362458 .. 50366489
4032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG275400.1

Sequence Viewer

Length: 441 bp
ATGCTTAGCGCATTAATTGTATCTCGTCCTGAGATGCTTGCCAAGCTATATGAGGAGATGACTGATCAAAAACCTCTCAGATTTAGGACTCCAGATGCAGTTGCAGCTCCTTCCGTCTTCATTGCCTGTAGACATGAAAATAAGCCACGTAGTGTGAAAGTGACAACCAGTTCTAGCCAACCTCAGCAACGGACAACCAAGGACAGCTATGGGTACTCCAAAGGTATGCCAGAATTCTTTCGAGGCCTTGTTGTGCCAGTTATCCACAATGCTGATAAGATGAATTGTGGTGAGATAGAGAATGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACATCTTTCAATTGATGATATGGCTGGAGGTTCATTTACAATATCCAATGGTGGATTTTATGGAAGCCTTATAAGTACCCCCATCATCAACCCTCATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

146

Amino Acids

16.03

Weight (kDa)

7.8

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 81 - 144 1.4e-21 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 410
AccI GTMKAC 1 cut(s) 130
AcsI RAATTY 1 cut(s) 233
AdeI CACNNNGTG 1 cut(s) 152
AfaI GTAC 2 cut(s) 215, 415
AgsI TTSAA 1 cut(s) 348
AluBI AGCT 3 cut(s) 46, 107, 207
AluI AGCT 3 cut(s) 46, 107, 207
AoxI GGCC 1 cut(s) 244
ApeKI GCWGC 1 cut(s) 104
ApoI RAATTY 1 cut(s) 233
AseI ATTAAT 1 cut(s) 14
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 109
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 116
BccI CCATC 1 cut(s) 428
BclI TGATCA 1 cut(s) 64
BfaI CTAG 1 cut(s) 174
BfmI CTRYAG 1 cut(s) 127
BisI GCNGC 1 cut(s) 105
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 106
BmsI GCATC 2 cut(s) 24, 85
BpiI GAAGAC 1 cut(s) 109
BpmI CTGGAG 2 cut(s) 75, 384
Bpu10I CCTNAGC 1 cut(s) 183
Bpu1102I GCTNAGC 1 cut(s) 5
BsaAI YACGTR 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 198
Bse1I ACTGG 2 cut(s) 168, 257
Bse3DI GCAATG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 198
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 3 cut(s) 21, 91, 197
BseNI ACTGG 2 cut(s) 168, 257
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 1 cut(s) 116
BshFI GGCC 1 cut(s) 246
BslFI GGGAC 1 cut(s) 351
BsmFI GGGAC 1 cut(s) 351
BsnI GGCC 1 cut(s) 246
Bsp143I GATC 2 cut(s) 64, 306
Bsp1720I GCTNAGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 246
BspCNI CTCAG 3 cut(s) 22, 90, 196
BsrDI GCAATG 1 cut(s) 120
BsrI ACTGG 2 cut(s) 168, 257
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 2 cut(s) 64, 306
BssT1I CCWWGG 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 39, 318
BstDEI CTNAG 4 cut(s) 5, 30, 77, 183
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 2 cut(s) 67, 309
BstMBI GATC 2 cut(s) 64, 306
BstMWI GCNNNNNNNGC 3 cut(s) 43, 104, 326
BstSFI CTRYAG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 116
BstV2I GAAGAC 1 cut(s) 109
BsuRI GGCC 1 cut(s) 246
Cac8I GCNNGC 2 cut(s) 39, 318
CfoI GCGC 1 cut(s) 11
Csp6I GTAC 2 cut(s) 214, 414
CviAII CATG 2 cut(s) 134, 434
CviJI RGCY 8 cut(s) 46, 107, 145, 177, 207, 246, 362, 405
CviKI_1 RGCY 8 cut(s) 46, 107, 145, 177, 207, 246, 362, 405
CviQI GTAC 2 cut(s) 214, 414
DdeI CTNAG 4 cut(s) 5, 30, 77, 183
DpnI GATC 2 cut(s) 66, 308
DpnII GATC 2 cut(s) 64, 306
DraIII CACNNNGTG 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 198
Eco147I AGGCCT 1 cut(s) 246
EcoRI GAATTC 1 cut(s) 233
EcoT14I CCWWGG 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 2 cut(s) 137, 437
FaiI YATR 9 cut(s) 49, 51, 135, 210, 227, 359, 399, 410, 435
FaqI GGGAC 1 cut(s) 351
FatI CATG 2 cut(s) 133, 433
FbaI TGATCA 1 cut(s) 64
FblI GTMKAC 1 cut(s) 130
Fnu4HI GCNGC 1 cut(s) 105
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 1 cut(s) 174
GlaI GCGC 1 cut(s) 10
GluI GCNGC 1 cut(s) 105
GsuI CTGGAG 2 cut(s) 75, 384
HaeIII GGCC 1 cut(s) 246
HhaI GCGC 1 cut(s) 11
Hin1II CATG 2 cut(s) 137, 437
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 1 cut(s) 88
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 1 cut(s) 131
Hpy188I TCNGA 2 cut(s) 80, 440
Hpy188III TCNNGA 2 cut(s) 29, 92
Hpy8I GTNNAC 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 120, 319
HpyCH4IV ACGT 1 cut(s) 148
HpyCH4V TGCA 3 cut(s) 98, 104, 320
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 104, 326
HpyF3I CTNAG 4 cut(s) 5, 30, 77, 183
HpySE526I ACGT 1 cut(s) 148
Hsp92II CATG 2 cut(s) 137, 437
HspAI GCGC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 2 cut(s) 64, 306
LmnI GCTCC 1 cut(s) 112
LpnPI CCDG 7 cut(s) 42, 105, 139, 181, 243, 270, 348
Lsp1109I GCAGC 1 cut(s) 116
LweI GCATC 2 cut(s) 24, 85
MaeI CTAG 1 cut(s) 174
MaeII ACGT 1 cut(s) 148
MaeIII GTNAC 1 cut(s) 160
MalI GATC 2 cut(s) 66, 308
MboI GATC 2 cut(s) 64, 306
MboII GAAGA 1 cut(s) 109
MfeI CAATTG 1 cut(s) 348
MluCI AATT 5 cut(s) 15, 233, 283, 310, 348
MlyI GAGTC 1 cut(s) 82
MnlI CCTC 7 cut(s) 46, 84, 192, 236, 328, 359, 441
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 1 cut(s) 14
MunI CAATTG 1 cut(s) 348
MwoI GCNNNNNNNGC 3 cut(s) 43, 104, 326
NdeII GATC 2 cut(s) 64, 306
NlaIII CATG 2 cut(s) 137, 437
NmuCI GTSAC 1 cut(s) 160
PceI AGGCCT 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 237
PkrI GCNGC 1 cut(s) 106
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
Ppu21I YACGTR 1 cut(s) 149
PshBI ATTAAT 1 cut(s) 14
PsiI TTATAA 1 cut(s) 410
RsaI GTAC 2 cut(s) 215, 415
RsaNI GTAC 2 cut(s) 214, 414
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 2 cut(s) 64, 306
SchI GAGTC 1 cut(s) 82
SetI ASST 8 cut(s) 48, 76, 109, 151, 184, 209, 226, 370
SfaNI GCATC 2 cut(s) 24, 85
SfcI CTRYAG 1 cut(s) 127
Sse9I AATT 5 cut(s) 15, 233, 283, 310, 348
SseBI AGGCCT 1 cut(s) 246
SspMI CTAG 1 cut(s) 174
StuI AGGCCT 1 cut(s) 246
StyI CCWWGG 1 cut(s) 198
TaiI ACGT 1 cut(s) 151
TaqI TCGA 1 cut(s) 241
TasI AATT 5 cut(s) 15, 233, 283, 310, 348
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 104
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 4 cut(s) 109, 150, 296, 360
TspGWI ACGGA 2 cut(s) 103, 205
VspI ATTAAT 1 cut(s) 14
XapI RAATTY 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 130
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.