Rh5BG276800

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000387
Physical Location & Seq
Forward (+)
34906788 .. 34910697
3910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG276800.1

Sequence Viewer

Length: 1032 bp
ATGCTTGGCGCTATAAGGCGAAGACTCGTCTCTGGAGCCTCCTCTCCTTCGACTTTGGGCCACTCATTGTCTTCAATCCGACCTATTGCAAACAAGGAGATTTTACCTCAAATCGGAGGAGGATTGGGTCATGTGCGCAATTATTCTCACCTCATTTTAGCTGGTTCATCGCATTGTATCAGAACTCCGAGGGATGTTGGAAGCTCTATTCAGCCAGAGCCTATCAGGCAAATGTGGAGCAGGCCCTTTTCTTCAGAGACTGGGGATTTGGTTGACGCCGTTGTGCCTTTTATGGGTGAATCTATTACTGATGGAACTTTGGCGACATTCTTGAAGAAGCCTGGAGACCGGGTGGAAGTTGATGAGCCGATTGCTCAAATAGAAACAGATAAGGTCACGATCGATGTTGCTAGTCCTGAATCTGGTGTAATTGAGAAGTTTGTAGCCAATGAAGGGGATACTGTGGAACCAGGCACCAAGATTGCTATCATACTAAAGTCCGGTGAAGGTGTATCCCATGTGGGTCCATCTGAGAAAACACTGGAGGAAGCTATTCCACAACCAGCTTCCCCTGTAGAAACAATCAGCAAGAATGAAACATCTAAAGTTGAAACAACTCCAGTTACCGATAAGCCTAAAGGACCCTCCCCACCATCTCCCAAACCCTCAGCTCAAGAACCTCAGCTCCCCCCTAAGGAACGAGAAAGACGAATTCCTATGACAACGCTTCGGAAACGAATTGCTAAAAGATTGAAAGATTCACAGAACACATATGCAATGCTTACAACATTCAATGAAGTTGATATGACAAATTTGATGAATCTTCGTTCTGACTTCAAGGATGCATTTGTTGAGAAGCATGGGGTCAAGTTGGGGCTTATGTCGGGATTCATCAAGGCTGCTGTCAGTGGGCTCCAAAATCAGCCTACTATTAATGCAGTCATTGATGGGGATGATATCATATATAGAGATTACATAGATATAAGTGTAGCCGTGGGTACTCCAAAGGTATGCCAGAATTCTTTCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

343

Amino Acids

37.1

Weight (kDa)

6.14

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 95 - 164 2.2e-17 Biotin-requiring enzyme
BSH_CusB PF25919 111 - 164 1e-05 CusB, barrel-sandwich hybrid domain
2-oxoacid_dh PF00198 233 - 336 1.9e-26 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 473
AcsI RAATTY 3 cut(s) 711, 811, 1018
AcuI CTGAAG 1 cut(s) 237
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 1 cut(s) 1000
AfiI CCNNNNNNNGG 5 cut(s) 113, 293, 422, 453, 694
AgsI TTSAA 6 cut(s) 75, 334, 611, 754, 793, 838
AjnI CCWGG 2 cut(s) 340, 469
AloI GAACNNNNNNTCC 2 cut(s) 669, 701
AluBI AGCT 6 cut(s) 161, 204, 551, 566, 671, 685
AluI AGCT 6 cut(s) 161, 204, 551, 566, 671, 685
Alw26I GTCTC 3 cut(s) 34, 251, 339
AlwNI CAGNNNCTG 1 cut(s) 260
AoxI GGCC 2 cut(s) 58, 242
ApeKI GCWGC 1 cut(s) 899
ApoI RAATTY 3 cut(s) 711, 811, 1018
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
AseI ATTAAT 1 cut(s) 933
Asp700I GAANNNNTTC 2 cut(s) 552, 1022
AspLEI GCGC 2 cut(s) 11, 138
AspS9I GGNCC 4 cut(s) 58, 243, 524, 641
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 3 cut(s) 140, 308, 515
AvaII GGWCC 2 cut(s) 524, 641
AxyI CCTNAGG 1 cut(s) 693
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 1 cut(s) 915
BbsI GAAGAC 2 cut(s) 28, 63
BbvCI CCTCAGC 2 cut(s) 667, 681
BbvI GCAGC 1 cut(s) 886
BccI CCATC 4 cut(s) 305, 535, 661, 941
BceAI ACGGC 2 cut(s) 263, 977
BciT130I CCWGG 2 cut(s) 342, 471
BciVI GTATCC 2 cut(s) 451, 523
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 3 cut(s) 34, 251, 339
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 573
BfoI RGCGCY 1 cut(s) 12
BfuI GTATCC 2 cut(s) 451, 523
BglI GCCNNNNNGGC 1 cut(s) 226
BisI GCNGC 1 cut(s) 900
BlsI GCNGC 1 cut(s) 901
Bme1390I CCNGG 3 cut(s) 342, 350, 471
Bme18I GGWCC 2 cut(s) 524, 641
BmgT120I GGNCC 4 cut(s) 58, 243, 524, 641
BmiI GGNNCC 6 cut(s) 37, 468, 475, 525, 643, 914
BmrFI CCNGG 3 cut(s) 342, 350, 471
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 832
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 28, 63
BpmI CTGGAG 4 cut(s) 54, 363, 563, 603
Bpu10I CCTNAGC 2 cut(s) 667, 681
BpuEI CTTGAG 1 cut(s) 657
BpuMI CCSGG 1 cut(s) 350
Bsa29I ATCGAT 1 cut(s) 402
BsaBI GATNNNNATC 1 cut(s) 485
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 188, 993
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 669, 699
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 293, 422, 453, 694
Bse1I ACTGG 3 cut(s) 265, 546, 620
Bse21I CCTNAGG 1 cut(s) 693
Bse3DI GCAATG 1 cut(s) 783
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 2 cut(s) 342, 471
BseCI ATCGAT 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 188, 993
BseGI GGATG 3 cut(s) 199, 847, 958
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 5 cut(s) 113, 293, 422, 453, 694
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 3 cut(s) 522, 681, 695
BseNI ACTGG 3 cut(s) 265, 546, 620
BseRI GAGGAG 2 cut(s) 31, 132
BseXI GCAGC 1 cut(s) 886
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 2 cut(s) 60, 244
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 402
BsiEI CGRYCG 1 cut(s) 402
BsiSI CCGG 2 cut(s) 349, 501
BslI CCNNNNNNNGG 5 cut(s) 113, 293, 422, 453, 694
BsmAI GTCTC 3 cut(s) 34, 251, 339
BsmBI CGTCTC 1 cut(s) 34
BsnI GGCC 2 cut(s) 60, 244
Bso31I GGTCTC 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 915
Bsp143I GATC 1 cut(s) 399
BspANI GGCC 2 cut(s) 60, 244
BspCNI CTCAG 3 cut(s) 523, 680, 694
BspDI ATCGAT 1 cut(s) 402
BspLI GGNNCC 6 cut(s) 37, 468, 475, 525, 643, 914
BspT107I GGYRCC 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 783
BsrI ACTGG 3 cut(s) 265, 546, 620
BssECI CCNNGG 2 cut(s) 188, 993
BssMI GATC 1 cut(s) 399
BssNI GRCGYC 1 cut(s) 276
Bst2UI CCWGG 2 cut(s) 342, 471
Bst4CI ACNGT 1 cut(s) 463
BstACI GRCGYC 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 4 cut(s) 531, 667, 681, 693
BstDSI CCRYGG 1 cut(s) 993
BstF5I GGATG 3 cut(s) 199, 847, 958
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 138
BstKTI GATC 1 cut(s) 402
BstMAI GTCTC 3 cut(s) 34, 251, 339
BstMBI GATC 1 cut(s) 399
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 2 cut(s) 342, 471
BstSCI CCNGG 3 cut(s) 340, 348, 469
BstSFI CTRYAG 1 cut(s) 573
BstV1I GCAGC 1 cut(s) 886
BstV2I GAAGAC 2 cut(s) 28, 63
Bsu15I ATCGAT 1 cut(s) 402
Bsu36I CCTNAGG 1 cut(s) 693
BsuI GTATCC 2 cut(s) 451, 523
BsuRI GGCC 2 cut(s) 60, 244
BsuTUI ATCGAT 1 cut(s) 402
BtgI CCRYGG 1 cut(s) 993
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 199, 847, 958
BtsIMutI CAGTG 2 cut(s) 539, 913
Cac8I GCNNGC 1 cut(s) 242
CaiI CAGNNNCTG 1 cut(s) 260
CfoI GCGC 2 cut(s) 11, 138
Cfr13I GGNCC 4 cut(s) 58, 243, 524, 641
ClaI ATCGAT 1 cut(s) 402
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 999
CviAII CATG 3 cut(s) 131, 518, 860
CviQI GTAC 1 cut(s) 999
DdeI CTNAG 4 cut(s) 531, 667, 681, 693
DpnI GATC 1 cut(s) 401
DpnII GATC 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 915
Eco31I GGTCTC 1 cut(s) 339
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 2 cut(s) 524, 641
Eco57I CTGAAG 1 cut(s) 237
Eco81I CCTNAGG 1 cut(s) 693
EcoO109I RGGNCCY 2 cut(s) 243, 641
EcoRI GAATTC 2 cut(s) 711, 1018
EcoRII CCWGG 2 cut(s) 340, 469
EcoRV GATATC 1 cut(s) 958
EcoT22I ATGCAT 1 cut(s) 847
EcoT38I GRGCYC 1 cut(s) 915
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 3 cut(s) 134, 521, 863
FatI CATG 3 cut(s) 130, 517, 859
FauNDI CATATG 1 cut(s) 772
Fnu4HI GCNGC 1 cut(s) 900
FokI GGATG 3 cut(s) 206, 854, 965
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 1 cut(s) 900
FspBI CTAG 1 cut(s) 411
FspI TGCGCA 1 cut(s) 137
GlaI GCGC 2 cut(s) 10, 137
GluI GCNGC 1 cut(s) 900
GsuI CTGGAG 4 cut(s) 54, 363, 563, 603
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 60, 244
HapII CCGG 2 cut(s) 349, 501
HgaI GACGC 1 cut(s) 284
HhaI GCGC 2 cut(s) 11, 138
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 3 cut(s) 134, 521, 863
Hin6I GCGC 2 cut(s) 9, 136
HinP1I GCGC 2 cut(s) 9, 136
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 6 cut(s) 24, 299, 419, 758, 820, 888
HpaII CCGG 2 cut(s) 349, 501
HphI GGTGA 3 cut(s) 140, 308, 515
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 8 cut(s) 80, 116, 182, 189, 256, 532, 732, 832
Hpy188III TCNNGA 6 cut(s) 33, 331, 397, 416, 674, 885
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 3 cut(s) 57, 446, 500
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 4 cut(s) 89, 776, 845, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 4 cut(s) 531, 667, 681, 693
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 3 cut(s) 134, 521, 863
HspAI GCGC 2 cut(s) 9, 136
Kzo9I GATC 1 cut(s) 399
LmnI GCTCC 4 cut(s) 35, 237, 690, 918
Lsp1109I GCAGC 1 cut(s) 886
LweI GCATC 1 cut(s) 832
MaeI CTAG 1 cut(s) 411
MaeIII GTNAC 2 cut(s) 394, 622
MalI GATC 1 cut(s) 401
MboI GATC 1 cut(s) 399
MboII GAAGA 5 cut(s) 33, 63, 243, 346, 815
MhlI GDGCHC 1 cut(s) 915
MluCI AATT 6 cut(s) 139, 429, 711, 738, 811, 1018
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 2 cut(s) 103, 178
Mph1103I ATGCAT 1 cut(s) 847
MroXI GAANNNNTTC 2 cut(s) 552, 1022
MseI TTAA 1 cut(s) 933
MspI CCGG 2 cut(s) 349, 501
MspR9I CCNGG 3 cut(s) 342, 350, 471
MvaI CCWGG 2 cut(s) 342, 471
MwoI GCNNNNNNNGC 1 cut(s) 226
NciI CCSGG 1 cut(s) 350
NdeI CATATG 1 cut(s) 772
NdeII GATC 1 cut(s) 399
NlaIII CATG 3 cut(s) 134, 521, 863
NlaIV GGNNCC 6 cut(s) 37, 468, 475, 525, 643, 914
NmuCI GTSAC 1 cut(s) 394
NsbI TGCGCA 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 847
PcsI WCGNNNNNNNCGW 1 cut(s) 706
PdmI GAANNNNTTC 2 cut(s) 552, 1022
PfeI GAWTC 5 cut(s) 299, 419, 758, 820, 888
PkrI GCNGC 1 cut(s) 901
Ple19I CGATCG 1 cut(s) 402
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
PpuMI RGGWCCY 1 cut(s) 641
PshBI ATTAAT 1 cut(s) 933
Psp5II RGGWCCY 1 cut(s) 641
Psp6I CCWGG 2 cut(s) 340, 469
PspGI CCWGG 2 cut(s) 340, 469
PspN4I GGNNCC 6 cut(s) 37, 468, 475, 525, 643, 914
PspPI GGNCC 4 cut(s) 58, 243, 524, 641
PspPPI RGGWCCY 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 260
PvuI CGATCG 1 cut(s) 402
RsaI GTAC 1 cut(s) 1000
RsaNI GTAC 1 cut(s) 999
SaqAI TTAA 1 cut(s) 933
SatI GCNGC 1 cut(s) 900
Sau3AI GATC 1 cut(s) 399
Sau96I GGNCC 4 cut(s) 58, 243, 524, 641
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 3 cut(s) 342, 350, 471
SduI GDGCHC 1 cut(s) 915
SfaNI GCATC 1 cut(s) 832
SfcI CTRYAG 1 cut(s) 573
SinI GGWCC 2 cut(s) 524, 641
SmlI CTYRAG 1 cut(s) 672
SmoI CTYRAG 1 cut(s) 672
Sse9I AATT 6 cut(s) 139, 429, 711, 738, 811, 1018
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 3 cut(s) 340, 348, 469
TaaI ACNGT 1 cut(s) 463
TaqI TCGA 3 cut(s) 50, 402, 1026
TasI AATT 6 cut(s) 139, 429, 711, 738, 811, 1018
TfiI GAWTC 5 cut(s) 299, 419, 758, 820, 888
Tru1I TTAA 1 cut(s) 933
Tru9I TTAA 1 cut(s) 933
TscAI CASTG 2 cut(s) 546, 913
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 1 cut(s) 899
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 6 cut(s) 156, 465, 609, 810, 833, 880
TspRI CASTG 2 cut(s) 546, 913
VpaK11BI GGWCC 2 cut(s) 524, 641
VspI ATTAAT 1 cut(s) 933
XapI RAATTY 3 cut(s) 711, 811, 1018
XmnI GAANNNNTTC 2 cut(s) 552, 1022
XspI CTAG 1 cut(s) 411
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.