Rh6BG136500

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000387
Physical Location & Seq
Reverse (-)
21672205 .. 21674960
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG136500.1

Sequence Viewer

Length: 600 bp
ATGGCGGGTAAGACCAACTCGGTGTCGATTCGGGTGTCGTACCGCAACTTGAAGGATACGAAAGTTGAAATGATAGGAATGGATGATGAGCCTCATCATCGGATCGACCTCAATTCCTCTAGGCCATCTCCATCGTCGTTGTCGTCGTCCTCCGCTCGGGTTGCCGCCGGTGTTCAGTTCGGTTGGGCTTTGCAGCTTTCGCTCTTAACTCCCTATATACAGGGGCGACGTGGGAGACTGGGAGATTTTGCACTTTCGGGCAGCTTCAACGACAAGAATGAGAAACTTCGTTGTGACTTCAAGGATGCATCTGTTGAGAAGCATGGGGTTAAGTTGGGGCTTATGTCGAGATTCATCAAGGCTGTTGTCAATATAAGTGCAGCTGTGGGTACTCCAAAGAGCCTTGTTGTGCCAATTATCCGCAATGCTAGTAAGATGAATTGTGGTGAGATTGAGAAGGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACGCCTTTCAATTGATGATATGGCTGGAGGTTCATTTACAATATCCAATGGTGGATTTTGTGGAAGCCTTATAAGTACCCCCATCATCAACCCTCCTCAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

199

Amino Acids

21.37

Weight (kDa)

9.6

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 120 - 199 4.5e-27 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AccBSI CCGCTC 1 cut(s) 155
AciI CCGC 5 cut(s) 5, 43, 153, 165, 421
AclWI GGATC 1 cut(s) 110
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 3 cut(s) 41, 391, 571
AfiI CCNNNNNNNGG 2 cut(s) 156, 592
AgsI TTSAA 5 cut(s) 52, 68, 268, 301, 504
AjiI CACGTC 1 cut(s) 230
AluBI AGCT 3 cut(s) 196, 264, 383
AluI AGCT 3 cut(s) 196, 264, 383
Alw26I GTCTC 1 cut(s) 229
AlwI GGATC 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 1 cut(s) 122
ApeKI GCWGC 3 cut(s) 193, 261, 380
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 156
AxyI CCTNAGG 1 cut(s) 591
BaeI ACNNNNGTAYC 2 cut(s) 48, 81
BbvI GCAGC 3 cut(s) 205, 273, 392
BccI CCATC 3 cut(s) 133, 139, 584
BciVI GTATCC 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 229
BfaI CTAG 2 cut(s) 120, 429
BfuI GTATCC 1 cut(s) 49
BisI GCNGC 4 cut(s) 165, 194, 262, 381
BlsI GCNGC 4 cut(s) 166, 195, 263, 382
BmeT110I CYCGRG 1 cut(s) 156
BmgBI CACGTC 1 cut(s) 230
BmrI ACTGGG 1 cut(s) 248
BmsI GCATC 2 cut(s) 295, 317
BmuI ACTGGG 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 496
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 592
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 243
Bse21I CCTNAGG 1 cut(s) 591
Bse3DI GCAATG 1 cut(s) 430
BseGI GGATG 2 cut(s) 88, 310
BseLI CCNNNNNNNGG 2 cut(s) 156, 592
BseMI GCAATG 1 cut(s) 430
BseNI ACTGG 1 cut(s) 243
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 3 cut(s) 205, 273, 392
BsgI GTGCAG 1 cut(s) 399
BshFI GGCC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 507
BslI CCNNNNNNNGG 2 cut(s) 156, 592
BsmAI GTCTC 1 cut(s) 229
BsmFI GGGAC 1 cut(s) 507
BsnI GGCC 1 cut(s) 124
BsoBI CYCGRG 1 cut(s) 156
Bsp143I GATC 2 cut(s) 102, 462
BspACI CCGC 5 cut(s) 5, 43, 153, 165, 421
BspANI GGCC 1 cut(s) 124
BspPI GGATC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 430
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 1 cut(s) 243
BssAI RCCGGY 1 cut(s) 167
BssMI GATC 2 cut(s) 102, 462
BssNI GRCGYC 1 cut(s) 496
BstACI GRCGYC 1 cut(s) 496
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 1 cut(s) 591
BstF5I GGATG 2 cut(s) 88, 310
BstKTI GATC 2 cut(s) 105, 465
BstMAI GTCTC 1 cut(s) 229
BstMBI GATC 2 cut(s) 102, 462
BstMWI GCNNNNNNNGC 3 cut(s) 161, 199, 482
BstV1I GCAGC 3 cut(s) 205, 273, 392
Bsu36I CCTNAGG 1 cut(s) 591
BsuI GTATCC 1 cut(s) 49
BsuRI GGCC 1 cut(s) 124
BtrI CACGTC 1 cut(s) 230
BtsCI GGATG 2 cut(s) 88, 310
Cac8I GCNNGC 1 cut(s) 474
Cfr10I RCCGGY 1 cut(s) 167
CseI GACGC 1 cut(s) 504
Csp6I GTAC 3 cut(s) 40, 390, 570
CviAII CATG 1 cut(s) 323
CviQI GTAC 3 cut(s) 40, 390, 570
DdeI CTNAG 1 cut(s) 591
DpnI GATC 2 cut(s) 104, 464
DpnII GATC 2 cut(s) 102, 462
Eco81I CCTNAGG 1 cut(s) 591
Eco88I CYCGRG 1 cut(s) 156
EcoT22I ATGCAT 1 cut(s) 310
FaeI CATG 1 cut(s) 326
FaiI YATR 7 cut(s) 216, 218, 324, 344, 374, 515, 566
FaqI GGGAC 1 cut(s) 507
FatI CATG 1 cut(s) 322
Fnu4HI GCNGC 4 cut(s) 165, 194, 262, 381
FokI GGATG 2 cut(s) 95, 317
Fsp4HI GCNGC 4 cut(s) 165, 194, 262, 381
FspBI CTAG 2 cut(s) 120, 429
GluI GCNGC 4 cut(s) 165, 194, 262, 381
GsuI CTGGAG 1 cut(s) 540
HaeIII GGCC 1 cut(s) 124
HapII CCGG 1 cut(s) 168
HgaI GACGC 1 cut(s) 504
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 1 cut(s) 326
HinfI GANTC 2 cut(s) 28, 351
HpaII CCGG 1 cut(s) 168
HphI GGTGA 1 cut(s) 458
Hpy188I TCNGA 1 cut(s) 102
Hpy188III TCNNGA 1 cut(s) 348
Hpy99I CGWCG 3 cut(s) 139, 148, 231
HpyAV CCTTC 3 cut(s) 46, 451, 475
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 5 cut(s) 193, 251, 308, 380, 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 199, 482
HpyF3I CTNAG 1 cut(s) 591
HpySE526I ACGT 1 cut(s) 229
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 1 cut(s) 326
Kzo9I GATC 2 cut(s) 102, 462
LpnPI CCDG 5 cut(s) 181, 206, 224, 504, 578
Lsp1109I GCAGC 3 cut(s) 205, 273, 392
LweI GCATC 2 cut(s) 295, 317
MaeI CTAG 2 cut(s) 120, 429
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 1 cut(s) 293
MalI GATC 2 cut(s) 104, 464
MbiI CCGCTC 1 cut(s) 155
MboI GATC 2 cut(s) 102, 462
MfeI CAATTG 1 cut(s) 504
MluCI AATT 5 cut(s) 112, 414, 439, 466, 504
MnlI CCTC 8 cut(s) 102, 119, 127, 160, 484, 515, 597, 600
Mph1103I ATGCAT 1 cut(s) 310
MseI TTAA 2 cut(s) 206, 330
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 1 cut(s) 168
MunI CAATTG 1 cut(s) 504
MwoI GCNNNNNNNGC 3 cut(s) 161, 199, 482
NdeII GATC 2 cut(s) 102, 462
NlaIII CATG 1 cut(s) 326
NmuCI GTSAC 1 cut(s) 293
NsiI ATGCAT 1 cut(s) 310
PcsI WCGNNNNNNNCGW 2 cut(s) 140, 143
PfeI GAWTC 2 cut(s) 28, 351
PkrI GCNGC 4 cut(s) 166, 195, 263, 382
PsiI TTATAA 1 cut(s) 566
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 3 cut(s) 41, 391, 571
RsaNI GTAC 3 cut(s) 40, 390, 570
SaqAI TTAA 2 cut(s) 206, 330
SatI GCNGC 4 cut(s) 165, 194, 262, 381
Sau3AI GATC 2 cut(s) 102, 462
SetI ASST 7 cut(s) 111, 198, 232, 266, 385, 526, 597
SfaNI GCATC 2 cut(s) 295, 317
SgrAI CRCCGGYG 1 cut(s) 167
Sse9I AATT 5 cut(s) 112, 414, 439, 466, 504
SsiI CCGC 5 cut(s) 5, 43, 153, 165, 421
SspMI CTAG 2 cut(s) 120, 429
TaiI ACGT 1 cut(s) 232
TaqI TCGA 3 cut(s) 26, 105, 347
TasI AATT 5 cut(s) 112, 414, 439, 466, 504
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 2 cut(s) 28, 351
Tru1I TTAA 2 cut(s) 206, 330
Tru9I TTAA 2 cut(s) 206, 330
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 3 cut(s) 193, 261, 380
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 3 cut(s) 343, 452, 516
XspI CTAG 2 cut(s) 120, 429
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.