Rw4G019560

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000387
Physical Location & Seq
Reverse (-)
42947158 .. 42948190
1033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019560.1

Sequence Viewer

Length: 432 bp
ATGCTTGCAACATTCAATGAAGTTGATATGACAAATTTGATGAAGCTTCGTTGTGACTTCAAGGATGCATCTGTTGAGAAGCATGGGGTCAAGTTGGGGCTTATGTCGAGATTCATCAAGGCTGCTGTCAGTGGGCTCCAAAATCAGCCTACTATTAATGCAGTCATTGATGGGGATGATATCATATATAGAGATTACATAGATATAAGTGCAGCTGTGGGTACTCCAAAGGGCCTTGTTGTGCCAGTTATCCGCAATGCTGATATGATGAATTGTGGTGAGATTGAGAAGGAGATCAATTCGCTTGCAAAGAAGGCAAATGAGGGACGTCTTTCAATTGATGATATGGCTGGAGGTTCGTTTACAATATCCAATTGTGGATTTTATGGAAGCCTTATAAGTACCCCCATCATCAACCCTCCTCAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

143

Amino Acids

15.46

Weight (kDa)

5.08

Isoelectric Point (pI)

24.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 3 - 143 3.8e-47 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 398
AatII GACGTC 1 cut(s) 331
AciI CCGC 1 cut(s) 253
AcsI RAATTY 1 cut(s) 34
AcyI GRCGYC 1 cut(s) 328
AfaI GTAC 2 cut(s) 223, 403
AfiI CCNNNNNNNGG 1 cut(s) 424
AgsI TTSAA 3 cut(s) 16, 61, 336
AluBI AGCT 2 cut(s) 46, 215
AluI AGCT 2 cut(s) 46, 215
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 2 cut(s) 122, 212
ApoI RAATTY 1 cut(s) 34
AseI ATTAAT 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 290
AxyI CCTNAGG 1 cut(s) 423
BanII GRGCYC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 109, 224
BccI CCATC 2 cut(s) 164, 416
BisI GCNGC 2 cut(s) 123, 213
BlsI GCNGC 2 cut(s) 124, 214
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 137
BmsI GCATC 2 cut(s) 55, 77
BpmI CTGGAG 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 328
Bsc4I CCNNNNNNNGG 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 245
Bse21I CCTNAGG 1 cut(s) 423
Bse3DI GCAATG 1 cut(s) 262
BseGI GGATG 2 cut(s) 70, 181
BseLI CCNNNNNNNGG 1 cut(s) 424
BseMI GCAATG 1 cut(s) 262
BseNI ACTGG 1 cut(s) 245
BseRI GAGGAG 1 cut(s) 411
BseXI GCAGC 2 cut(s) 109, 224
BsgI GTGCAG 1 cut(s) 231
BshFI GGCC 1 cut(s) 234
BslFI GGGAC 1 cut(s) 339
BslI CCNNNNNNNGG 1 cut(s) 424
BsmFI GGGAC 1 cut(s) 339
BsnI GGCC 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 1 cut(s) 294
BspACI CCGC 1 cut(s) 253
BspANI GGCC 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 1 cut(s) 245
BssMI GATC 1 cut(s) 294
BssNI GRCGYC 1 cut(s) 328
BstACI GRCGYC 1 cut(s) 328
BstC8I GCNNGC 2 cut(s) 6, 306
BstDEI CTNAG 1 cut(s) 423
BstF5I GGATG 2 cut(s) 70, 181
BstKTI GATC 1 cut(s) 297
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstV1I GCAGC 2 cut(s) 109, 224
Bsu36I CCTNAGG 1 cut(s) 423
BsuRI GGCC 1 cut(s) 234
BtsCI GGATG 2 cut(s) 70, 181
BtsIMutI CAGTG 1 cut(s) 136
Cac8I GCNNGC 2 cut(s) 6, 306
Cfr13I GGNCC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 222, 402
CviAII CATG 1 cut(s) 83
CviJI RGCY 9 cut(s) 46, 100, 122, 136, 148, 215, 234, 350, 393
CviKI_1 RGCY 9 cut(s) 46, 100, 122, 136, 148, 215, 234, 350, 393
CviQI GTAC 2 cut(s) 222, 402
DdeI CTNAG 1 cut(s) 423
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 138
Eco32I GATATC 1 cut(s) 181
Eco81I CCTNAGG 1 cut(s) 423
EcoO109I RGGNCCY 1 cut(s) 232
EcoRV GATATC 1 cut(s) 181
EcoT22I ATGCAT 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 138
FaeI CATG 1 cut(s) 86
FaqI GGGAC 1 cut(s) 339
FatI CATG 1 cut(s) 82
Fnu4HI GCNGC 2 cut(s) 123, 213
FokI GGATG 2 cut(s) 77, 188
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 123, 213
GluI GCNGC 2 cut(s) 123, 213
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 328
Hin1II CATG 1 cut(s) 86
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 1 cut(s) 111
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 1 cut(s) 431
Hpy188III TCNNGA 1 cut(s) 108
Hpy8I GTNNAC 1 cut(s) 363
HpyAV CCTTC 2 cut(s) 283, 307
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 5 cut(s) 8, 68, 161, 212, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 328
Hsp92I GRCGYC 1 cut(s) 328
Hsp92II CATG 1 cut(s) 86
Kzo9I GATC 1 cut(s) 294
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 3 cut(s) 258, 336, 410
Lsp1109I GCAGC 2 cut(s) 109, 224
LweI GCATC 2 cut(s) 55, 77
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 1 cut(s) 53
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MfeI CAATTG 2 cut(s) 336, 373
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 5 cut(s) 34, 271, 298, 336, 373
MnlI CCTC 4 cut(s) 316, 347, 429, 432
Mph1103I ATGCAT 1 cut(s) 70
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 215
MunI CAATTG 2 cut(s) 336, 373
MwoI GCNNNNNNNGC 1 cut(s) 314
NdeII GATC 1 cut(s) 294
NlaIII CATG 1 cut(s) 86
NlaIV GGNNCC 1 cut(s) 137
NmuCI GTSAC 1 cut(s) 53
NsiI ATGCAT 1 cut(s) 70
PfeI GAWTC 1 cut(s) 111
PkrI GCNGC 2 cut(s) 124, 214
PshBI ATTAAT 1 cut(s) 156
PsiI TTATAA 1 cut(s) 398
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 2 cut(s) 223, 403
RsaNI GTAC 2 cut(s) 222, 402
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 2 cut(s) 123, 213
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 1 cut(s) 232
SduI GDGCHC 1 cut(s) 138
SetI ASST 5 cut(s) 48, 217, 331, 358, 429
SfaNI GCATC 2 cut(s) 55, 77
Sse9I AATT 5 cut(s) 34, 271, 298, 336, 373
SsiI CCGC 1 cut(s) 253
TaiI ACGT 1 cut(s) 331
TaqI TCGA 1 cut(s) 107
TasI AATT 5 cut(s) 34, 271, 298, 336, 373
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 53
TseI GCWGC 2 cut(s) 122, 212
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 4 cut(s) 33, 56, 103, 284
TspRI CASTG 1 cut(s) 136
VspI ATTAAT 1 cut(s) 156
XapI RAATTY 1 cut(s) 34
ZraI GACGTC 1 cut(s) 329
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.