pycom03g14960

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000387
Physical Location & Seq
Reverse (-)
16341902 .. 16343493
1592 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g14960.5

Sequence Viewer

Length: 618 bp
ATGCTGACGACATTCAATGAAGTTGATATGACCAATTTGATGAAGCTTCGTTCTGAGTACAAGGATGCATTTGTTGAGAAGCACGGAGTCAAGTTGGGGCTTATGTCTGGATTTATCAAAGCTGCTGTCAGTGGTCTCCAAAACCAGCCTACTATTAATGCAGTCATTGATGGGGACGATATCATATACAGAGATTACATAGATATAAGTATAGCCGTTGGCACTCCAAAGGGCCTTGTTGTGCCAGTTATCCGCAATGCTGATAAAATGAATTTTGCTGAGATAGAGAAGGAGATCAACACCCTCGCAAAGAAAGCAAACGATGGATCTATATCGATCGACGAGATGGCTGGAGGTACATTTACAATATCCAATGGTGGAGTTTATGGAAGCCTTCTAAGTACCCCCATCATCAACCCCCCTCAGTCGGCAATCTTGGGCATGCACTCAATGGTTAACCGTCCAATGGTTGTCGGAGGCAACATTGTCCCGCGGCCGATGATGTACGTTGCTCTTACCTATGACCATAGGCTCATTGATGGAAGAGAGGCAGTTTACTTCTTGCGTCGTATTAAAGATGTTGTGGAGGACCCTCGGAGGCTTCTCCTCGACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00310 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl

Protein Analysis

206

Amino Acids

22.57

Weight (kDa)

5.93

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26910 AT4G26910 AT4G26910 AT5G55070 AT5G55070
fragaria_vesca FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_3g23070 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960 FvH4_6g42960
malus_domestica MD03G1198100.v1.1 MD09G1110700.v1.1 MD17G1098600.v1.1
prunus_persica Prupe.3G215800_v2.0.a1 Prupe.4G216900_v2.0.a1 Prupe.4G216900_v2.0.a1
pyrus_communis pycom03g14960 pycom09g03320 pycom17g09400
rosa_chinensis RchiOBHm_Chr2g0119431 RchiOBHm_Chr2g0119441 RchiOBHm_Chr2g0158941 RchiOBHm_Chr5g0040581 RchiOBHm_Chr5g0052421 RchiOBHm_Chr5g0052431
rosa_laevigata RLG00000002270 RLG00000012296 RLG00000021125 RLG00000031115 RLG00000033989
rosa_multiflora Rmu_co8099772.1_g000001 Rmu_co8181026.1_g000001 Rmu_co8403685.1_g000001 Rmu_sc0001893.1_g000003 Rmu_sc0004787.1_g000003 Rmu_sc0007967.1_g000007 Rmu_sc0010369.1_g000001 Rmu_ssc0000174.1_g000008
rosa_roxburghii Rroxscaffold_1G00040090 Rroxscaffold_1G00040800 Rroxscaffold_1G00049000 Rroxscaffold_2G00090860 Rroxscaffold_2G00122640 Rroxscaffold_2G00127540 Rroxscaffold_2G00128360 Rroxscaffold_3G00249890 Rroxscaffold_4G00314140 Rroxscaffold_5G00345820 Rroxscaffold_5G00345830 Rroxscaffold_5G00371680 Rroxscaffold_7G00164980 Rroxscaffold_7G00209200
rosa_rugosa Rorug01G0153500.1 Rorug02G0473100 Rorug02G0473200 Rorug02G0473200 Rorug03G0343200 Rorug05G0185800
rosa_samantha Rh1AG155300 Rh1AG275400 Rh1BG123600 Rh1CG026200 Rh1CG145600 Rh2AG539600 Rh2BG510900 Rh2BG552500 Rh2CG522900 Rh2DG522600 Rh2DG522700 Rh2DG562200 Rh3CG234300 Rh3CG302000 Rh3CG302100 Rh4DG305100 Rh5AG250900 Rh5AG272600 Rh5AG475300 Rh5BG276800 Rh5BG355700 Rh5CG308700 Rh5DG285400 Rh6BG136500 Rh6BG167900 Rh6BG220000 Rh7AG327600 Rh7AG349200 Rh7AG349300 Rh7AG349400 Rh7AG353200 Rh7CG344900 Rh7CG366500 Rh7CG366600
rosa_wichuraiana Rw1G002110 Rw2G044660 Rw3G008210 Rw4G016310 Rw4G019560 Rw5G025520 Rw5G030020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 493
AciI CCGC 3 cut(s) 253, 491, 493
AclWI GGATC 1 cut(s) 334
AcoI YGGCCR 1 cut(s) 494
AcsI RAATTY 1 cut(s) 271
AfaI GTAC 4 cut(s) 59, 358, 403, 506
AfiI CCNNNNNNNGG 2 cut(s) 427, 466
AgsI TTSAA 1 cut(s) 16
AluBI AGCT 2 cut(s) 46, 122
AluI AGCT 2 cut(s) 46, 122
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 1 cut(s) 334
AoxI GGCC 2 cut(s) 232, 494
ApeKI GCWGC 1 cut(s) 122
ApoI RAATTY 1 cut(s) 271
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
AseI ATTAAT 1 cut(s) 156
AspS9I GGNCC 2 cut(s) 232, 589
AvaII GGWCC 1 cut(s) 589
BbvI GCAGC 1 cut(s) 109
BccI CCATC 5 cut(s) 164, 317, 340, 416, 533
BceAI ACGGC 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 140
BisI GCNGC 2 cut(s) 123, 494
BlsI GCNGC 2 cut(s) 124, 495
Bme18I GGWCC 1 cut(s) 589
BmgT120I GGNCC 2 cut(s) 232, 589
BmiI GGNNCC 1 cut(s) 591
BmsI GCATC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 372
Bsa29I ATCGAT 1 cut(s) 335
BsaBI GATNNNNATC 1 cut(s) 331
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 2 cut(s) 491, 593
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 466
Bse1I ACTGG 1 cut(s) 245
Bse3DI GCAATG 1 cut(s) 262
Bse8I GATNNNNATC 1 cut(s) 331
BseCI ATCGAT 1 cut(s) 335
BseDI CCNNGG 2 cut(s) 491, 593
BseGI GGATG 1 cut(s) 70
BseJI GATNNNNATC 1 cut(s) 331
BseLI CCNNNNNNNGG 2 cut(s) 427, 466
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 3 cut(s) 45, 270, 437
BseNI ACTGG 1 cut(s) 245
BseRI GAGGAG 1 cut(s) 596
BseX3I CGGCCG 1 cut(s) 494
BseXI GCAGC 1 cut(s) 109
Bsh1236I CGCG 1 cut(s) 493
Bsh1285I CGRYCG 2 cut(s) 339, 497
BshFI GGCC 2 cut(s) 234, 496
BshVI ATCGAT 1 cut(s) 335
BsiEI CGRYCG 2 cut(s) 339, 497
BslFI GGGAC 2 cut(s) 188, 473
BslI CCNNNNNNNGG 2 cut(s) 427, 466
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 2 cut(s) 188, 473
BsnI GGCC 2 cut(s) 234, 496
Bso31I GGTCTC 1 cut(s) 140
Bsp143I GATC 3 cut(s) 294, 326, 336
BspACI CCGC 3 cut(s) 253, 491, 493
BspANI GGCC 2 cut(s) 234, 496
BspCNI CTCAG 3 cut(s) 46, 271, 436
BspDI ATCGAT 1 cut(s) 335
BspFNI CGCG 1 cut(s) 493
BspLI GGNNCC 1 cut(s) 591
BspPI GGATC 1 cut(s) 334
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 491, 593
BssMI GATC 3 cut(s) 294, 326, 336
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 1 cut(s) 538
BstC8I GCNNGC 1 cut(s) 443
BstDEI CTNAG 4 cut(s) 54, 279, 398, 423
BstDSI CCRYGG 1 cut(s) 491
BstF5I GGATG 1 cut(s) 70
BstFNI CGCG 1 cut(s) 493
BstKTI GATC 3 cut(s) 297, 329, 339
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 3 cut(s) 294, 326, 336
BstMCI CGRYCG 2 cut(s) 339, 497
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNSI RCATGY 1 cut(s) 445
BstUI CGCG 1 cut(s) 493
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
BstZI CGGCCG 1 cut(s) 494
Bsu15I ATCGAT 1 cut(s) 335
BsuRI GGCC 2 cut(s) 234, 496
BsuTUI ATCGAT 1 cut(s) 335
BtgI CCRYGG 1 cut(s) 491
BtsCI GGATG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 136
Cac8I GCNNGC 1 cut(s) 443
Cfr13I GGNCC 2 cut(s) 232, 589
Cfr42I CCGCGG 1 cut(s) 494
ClaI ATCGAT 1 cut(s) 335
CseI GACGC 1 cut(s) 554
Csp6I GTAC 4 cut(s) 58, 357, 402, 505
CviAII CATG 1 cut(s) 442
CviQI GTAC 4 cut(s) 58, 357, 402, 505
DdeI CTNAG 4 cut(s) 54, 279, 398, 423
DpnI GATC 3 cut(s) 296, 328, 338
DpnII GATC 3 cut(s) 294, 326, 336
EaeI YGGCCR 1 cut(s) 494
EagI CGGCCG 1 cut(s) 494
Eam1104I CTCTTC 1 cut(s) 538
EarI CTCTTC 1 cut(s) 538
EclXI CGGCCG 1 cut(s) 494
Eco31I GGTCTC 1 cut(s) 140
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 589
Eco52I CGGCCG 1 cut(s) 494
EcoO109I RGGNCCY 2 cut(s) 232, 589
EcoRV GATATC 1 cut(s) 181
EcoT22I ATGCAT 1 cut(s) 70
FaeI CATG 1 cut(s) 445
FaqI GGGAC 2 cut(s) 188, 473
FatI CATG 1 cut(s) 441
FauI CCCGC 1 cut(s) 498
Fnu4HI GCNGC 2 cut(s) 123, 494
FokI GGATG 1 cut(s) 77
Fsp4HI GCNGC 2 cut(s) 123, 494
GluI GCNGC 2 cut(s) 123, 494
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 2 cut(s) 234, 496
HgaI GACGC 1 cut(s) 554
Hin1II CATG 1 cut(s) 445
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 1 cut(s) 87
HpaI GTTAAC 1 cut(s) 457
Hpy166II GTNNAC 2 cut(s) 457, 556
Hpy188I TCNGA 3 cut(s) 55, 476, 597
Hpy188III TCNNGA 1 cut(s) 108
Hpy8I GTNNAC 2 cut(s) 457, 556
Hpy99I CGWCG 3 cut(s) 344, 570, 614
HpyAV CCTTC 2 cut(s) 283, 404
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 2 cut(s) 507, 612
HpyCH4V TGCA 3 cut(s) 68, 161, 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 4 cut(s) 54, 279, 398, 423
HpySE526I ACGT 2 cut(s) 507, 612
Hsp92II CATG 1 cut(s) 445
KspAI GTTAAC 1 cut(s) 457
KspI CCGCGG 1 cut(s) 494
Kzo9I GATC 3 cut(s) 294, 326, 336
LpnPI CCDG 4 cut(s) 93, 158, 258, 336
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 55
MaeII ACGT 2 cut(s) 507, 612
MalI GATC 3 cut(s) 296, 328, 338
MboI GATC 3 cut(s) 294, 326, 336
MboII GAAGA 1 cut(s) 555
MflI RGATCY 1 cut(s) 326
MluCI AATT 2 cut(s) 34, 271
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 454
MnlI CCTC 9 cut(s) 314, 347, 432, 470, 541, 580, 591, 603, 617
Mph1103I ATGCAT 1 cut(s) 70
MseI TTAA 3 cut(s) 156, 456, 573
MspA1I CMGCKG 1 cut(s) 493
MvnI CGCG 1 cut(s) 493
MwoI GCNNNNNNNGC 1 cut(s) 314
NdeII GATC 3 cut(s) 294, 326, 336
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 1 cut(s) 591
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 1 cut(s) 445
PaeI GCATGC 1 cut(s) 445
PkrI GCNGC 2 cut(s) 124, 495
Ple19I CGATCG 1 cut(s) 339
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PpuMI RGGWCCY 1 cut(s) 589
PshBI ATTAAT 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 589
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 2 cut(s) 232, 589
PspPPI RGGWCCY 1 cut(s) 589
PsuI RGATCY 1 cut(s) 326
PvuI CGATCG 1 cut(s) 339
RsaI GTAC 4 cut(s) 59, 358, 403, 506
RsaNI GTAC 4 cut(s) 58, 357, 402, 505
SacII CCGCGG 1 cut(s) 494
SaqAI TTAA 3 cut(s) 156, 456, 573
SatI GCNGC 2 cut(s) 123, 494
Sau3AI GATC 3 cut(s) 294, 326, 336
Sau96I GGNCC 2 cut(s) 232, 589
SchI GAGTC 1 cut(s) 96
SetI ASST 6 cut(s) 48, 124, 358, 510, 521, 615
SfaNI GCATC 1 cut(s) 55
Sfr303I CCGCGG 1 cut(s) 494
SgrBI CCGCGG 1 cut(s) 494
SinI GGWCC 1 cut(s) 589
SphI GCATGC 1 cut(s) 445
Sse9I AATT 2 cut(s) 34, 271
SsiI CCGC 3 cut(s) 253, 491, 493
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 2 cut(s) 510, 615
TaqI TCGA 3 cut(s) 335, 339, 609
TasI AATT 2 cut(s) 34, 271
TatI WGTACW 1 cut(s) 57
TauI GCSGC 1 cut(s) 496
Tru1I TTAA 3 cut(s) 156, 456, 573
Tru9I TTAA 3 cut(s) 156, 456, 573
TscAI CASTG 1 cut(s) 136
TseI GCWGC 1 cut(s) 122
TspDTI ATGAA 3 cut(s) 33, 56, 284
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 589
VspI ATTAAT 1 cut(s) 156
XapI RAATTY 1 cut(s) 271
XceI RCATGY 1 cut(s) 445
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.