Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0006529

Physical Location & Seq

Genomic Coordinates Forward (+)

11997619 .. 11999641

Gene Structure 2023 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1134300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 837 bp

ATGCTGCTAAGACATAAGAAAACTACAAATGGATGGATGTATACATGTGAGCAGCCCCACCCACCCTCCACTCCAATTTTAAATTCTTCCATCCAATTGTTGGAGTGCTATATAATAAGAAGTAAAGAACCAGAAGCTGGAGTTTGCAGATTTGATCTAAATCCTTCGGTTTTAACACTAAGAAGTCATAACATGGTGGTGAAGGTGTATGGGCCTCTGTACGCCGCCCCAAAGCGTGTGCTGGTATGCCTTGTTGAGAAAGAGGTTCACTTTGAGACAGTCCCCATTGATCTTCTCAAGGGAGAGAACAAACACCCTGATTTCCTCAAGTTACAGCCATTTGGAACAGTTCCTCTCATTCAAGATGGGGATTATACTCTATATGAATCACGCGCAATCATGAGGTACTACGCAGAAAAATATAAGTCACAAGGGACTGATTTGCTGGGAAAGACAATTGAGGAAAGAGGGCTTGTAGAACAATGGCTGGAAGTTGAAGCACACAACTTCCAGCCACCAATCTACAACTTGGTCGTTCACATTCTCTTTGCCCCAGTTTTGGGTTTTCCTTCAGACCCCAAGATCTTACAAGAGAGTGAAGAAAAGCTGGGAAAAGTGCTGGACATTTACGAGGAGCAGCTGTCGAAGAGCAAGTACTTGGCCGGTGATTTCTTCAGCCTTGCTGATATTAGCCACCTTCCATTCACTCACTTCTTGGTGGCTAATATGGGGAAGGAGTATATGATCAAGGACAGAAAGCATGTCTCTGCATGGTGGGATGACATTAGCAACAGACCATCTTGGAAGAGGGTCCTACAGTTTGGTGACCCATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0004601 GO:0004602 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009056 GO:0009404 GO:0009407 GO:0009414 GO:0009415 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016209 GO:0016491 GO:0016684 GO:0016740 GO:0016765 GO:0019748 GO:0030054 GO:0030312 GO:0033218 GO:0034641 GO:0042221 GO:0042277 GO:0042742 GO:0043167 GO:0043168 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048037 GO:0048046 GO:0050896 GO:0051186 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0055114 GO:0070887 GO:0071704 GO:0071944 GO:0072341 GO:0097237 GO:0098542 GO:0098754 GO:0098869 GO:1900750 GO:1901564 GO:1901681 GO:1901700 GO:1990748

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_N

Protein Analysis

279

Amino Acids

32.12

Weight (kDa)

6.37

Isoelectric Point (pI)

53.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	65 - 138	6.3e-16	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	78 - 139	1.2e-12	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	78 - 142	3.2e-09	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	183 - 266	2.3e-13	Glutathione S-transferase, C-terminal domain
GST_C_3	PF14497	199 - 268	5.7e-06	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006529)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30860 AT2G30860
fragaria_vesca	FvH4_6g38760
malus_domestica	MD09G1147100.v1.1 MD09G1147300.v1.1 MD09G1147400.v1.1 MD17G1133600.v1.1 MD17G1133800.v1.1 MD17G1134300.v1.1
prunus_persica	Prupe.3G178100_v2.0.a1 Prupe.3G178200_v2.0.a1 Prupe.3G178300_v2.0.a1
pyrus_communis	pycom09g06760 pycom17g12830 pycom17g12850
rosa_chinensis	RchiOBHm_Chr2g0152311
rosa_laevigata	RLG00000020627
rosa_multiflora	Rmu_sc0004002.1_g000002
rosa_rugosa	Rorug02G0432000 Rorug02G0432100 Rorug02G0432200
rosa_samantha	Rh2AG493800 Rh2BG506100 Rh2CG480100 Rh2DG517200
rosa_wichuraiana	Rw2G040610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	100, 137
AccI	GTMKAC	1 cut(s)	41
AccII	CGCG	1 cut(s)	393
AciI	CCGC	1 cut(s)	225
AcoI	YGGCCR	1 cut(s)	660
AcsI	RAATTY	1 cut(s)	82
AcuI	CTGAAG	2 cut(s)	555, 658
AfaI	GTAC	3 cut(s)	221, 407, 656
AfiI	CCNNNNNNNGG	4 cut(s)	100, 137, 559, 560
AflIII	ACRYGT	1 cut(s)	44
AgsI	TTSAA	2 cut(s)	362, 497
AjuI	GAANNNNNNNTTGG	2 cut(s)	67, 99
AluBI	AGCT	3 cut(s)	137, 607, 640
AluI	AGCT	3 cut(s)	137, 607, 640
Alw26I	GTCTC	2 cut(s)	269, 769
AlwNI	CAGNNNCTG	1 cut(s)	137
AoxI	GGCC	2 cut(s)	212, 660
ApeKI	GCWGC	3 cut(s)	4, 52, 637
ApoI	RAATTY	1 cut(s)	82
AspLEI	GCGC	1 cut(s)	395
AspS9I	GGNCC	2 cut(s)	212, 811
AsuHPI	GGTGA	3 cut(s)	211, 677, 836
AvaII	GGWCC	1 cut(s)	811
BarI	GAAGNNNNNNTAC	2 cut(s)	638, 670
BbvI	GCAGC	2 cut(s)	64, 649
BccI	CCATC	4 cut(s)	27, 98, 359, 805
BclI	TGATCA	1 cut(s)	744
BcoDI	GTCTC	2 cut(s)	269, 769
BfmI	CTRYAG	1 cut(s)	815
BglII	AGATCT	1 cut(s)	582
BisI	GCNGC	4 cut(s)	5, 53, 225, 638
BlsI	GCNGC	4 cut(s)	6, 54, 226, 639
BmcAI	AGTACT	1 cut(s)	656
Bme18I	GGWCC	1 cut(s)	811
BmgT120I	GGNCC	2 cut(s)	212, 811
BmiI	GGNNCC	1 cut(s)	812
BmrI	ACTGGG	1 cut(s)	548
BmuI	ACTGGG	1 cut(s)	548
BpmI	CTGGAG	1 cut(s)	159
BpuEI	CTTGAG	2 cut(s)	281, 311
BsaBI	GATNNNNATC	1 cut(s)	159
BsaXI	ACNNNNNCTCC	4 cut(s)	50, 80, 626, 656
Bsc4I	CCNNNNNNNGG	4 cut(s)	100, 137, 559, 560
Bse118I	RCCGGY	1 cut(s)	662
Bse1I	ACTGG	1 cut(s)	554
Bse8I	GATNNNNATC	1 cut(s)	159
BseGI	GGATG	4 cut(s)	38, 42, 90, 784
BseJI	GATNNNNATC	1 cut(s)	159
BseLI	CCNNNNNNNGG	4 cut(s)	100, 137, 559, 560
BseNI	ACTGG	1 cut(s)	554
BseRI	GAGGAG	1 cut(s)	647
BseXI	GCAGC	2 cut(s)	64, 649
BseYI	CCCAGC	2 cut(s)	445, 607
Bsh1236I	CGCG	1 cut(s)	393
BshFI	GGCC	2 cut(s)	214, 662
BsiSI	CCGG	1 cut(s)	663
BslFI	GGGAC	2 cut(s)	266, 448
BslI	CCNNNNNNNGG	4 cut(s)	100, 137, 559, 560
BsmAI	GTCTC	2 cut(s)	269, 769
BsmFI	GGGAC	2 cut(s)	266, 448
BsnI	GGCC	2 cut(s)	214, 662
Bsp143I	GATC	4 cut(s)	154, 289, 582, 744
BspACI	CCGC	1 cut(s)	225
BspANI	GGCC	2 cut(s)	214, 662
BspFNI	CGCG	1 cut(s)	393
BspHI	TCATGA	1 cut(s)	399
BspLI	GGNNCC	1 cut(s)	812
BspQI	GCTCTTC	1 cut(s)	641
BsrFI	RCCGGY	1 cut(s)	662
BsrI	ACTGG	1 cut(s)	554
BssAI	RCCGGY	1 cut(s)	662
BssMI	GATC	4 cut(s)	154, 289, 582, 744
BssNAI	GTATAC	1 cut(s)	42
Bst1107I	GTATAC	1 cut(s)	42
Bst4CI	ACNGT	3 cut(s)	280, 349, 819
Bst6I	CTCTTC	2 cut(s)	641, 800
BstDEI	CTNAG	2 cut(s)	8, 179
BstEII	GGTNACC	1 cut(s)	824
BstF5I	GGATG	4 cut(s)	38, 42, 90, 784
BstFNI	CGCG	1 cut(s)	393
BstHHI	GCGC	1 cut(s)	395
BstKTI	GATC	4 cut(s)	157, 292, 585, 747
BstMAI	GTCTC	2 cut(s)	269, 769
BstMBI	GATC	4 cut(s)	154, 289, 582, 744
BstNSI	RCATGY	2 cut(s)	48, 764
BstPI	GGTNACC	1 cut(s)	824
BstSFI	CTRYAG	1 cut(s)	815
BstUI	CGCG	1 cut(s)	393
BstV1I	GCAGC	2 cut(s)	64, 649
BstX2I	RGATCY	1 cut(s)	582
BstYI	RGATCY	1 cut(s)	582
BstZ17I	GTATAC	1 cut(s)	42
BsuRI	GGCC	2 cut(s)	214, 662
BtsCI	GGATG	4 cut(s)	38, 42, 90, 784
CaiI	CAGNNNCTG	1 cut(s)	137
CciI	TCATGA	1 cut(s)	399
CfoI	GCGC	1 cut(s)	395
Cfr10I	RCCGGY	1 cut(s)	662
Cfr13I	GGNCC	2 cut(s)	212, 811
Csp6I	GTAC	3 cut(s)	220, 406, 655
CviAII	CATG	5 cut(s)	45, 193, 400, 761, 771
CviQI	GTAC	3 cut(s)	220, 406, 655
DdeI	CTNAG	2 cut(s)	8, 179
DpnI	GATC	4 cut(s)	156, 291, 584, 746
DpnII	GATC	4 cut(s)	154, 289, 582, 744
DraI	TTTAAA	1 cut(s)	81
EaeI	YGGCCR	1 cut(s)	660
Eam1104I	CTCTTC	2 cut(s)	641, 800
EarI	CTCTTC	2 cut(s)	641, 800
Eco47I	GGWCC	1 cut(s)	811
Eco57I	CTGAAG	2 cut(s)	555, 658
Eco91I	GGTNACC	1 cut(s)	824
EcoO109I	RGGNCCY	1 cut(s)	811
EcoO65I	GGTNACC	1 cut(s)	824
FaeI	CATG	5 cut(s)	48, 196, 403, 764, 774
FaqI	GGGAC	2 cut(s)	266, 448
FatI	CATG	5 cut(s)	44, 192, 399, 760, 770
FbaI	TGATCA	1 cut(s)	744
FblI	GTMKAC	1 cut(s)	41
Fnu4HI	GCNGC	4 cut(s)	5, 53, 225, 638
FokI	GGATG	4 cut(s)	45, 49, 77, 791
Fsp4HI	GCNGC	4 cut(s)	5, 53, 225, 638
GlaI	GCGC	1 cut(s)	394
GluI	GCNGC	4 cut(s)	5, 53, 225, 638
GsaI	CCCAGC	2 cut(s)	449, 611
GsuI	CTGGAG	1 cut(s)	159
HaeIII	GGCC	2 cut(s)	214, 662
HapII	CCGG	1 cut(s)	663
HhaI	GCGC	1 cut(s)	395
Hin1II	CATG	5 cut(s)	48, 196, 403, 764, 774
Hin6I	GCGC	1 cut(s)	393
HinP1I	GCGC	1 cut(s)	393
HinfI	GANTC	1 cut(s)	386
HpaII	CCGG	1 cut(s)	663
HphI	GGTGA	3 cut(s)	211, 677, 836
Hpy166II	GTNNAC	3 cut(s)	42, 268, 538
Hpy188I	TCNGA	1 cut(s)	574
Hpy188III	TCNNGA	2 cut(s)	362, 400
Hpy8I	GTNNAC	3 cut(s)	42, 268, 538
HpyAV	CCTTC	5 cut(s)	174, 196, 579, 707, 727
HpyCH4III	ACNGT	3 cut(s)	280, 349, 819
HpyCH4V	TGCA	2 cut(s)	147, 770
HpyF3I	CTNAG	2 cut(s)	8, 179
Hsp92II	CATG	5 cut(s)	48, 196, 403, 764, 774
HspAI	GCGC	1 cut(s)	393
Ksp22I	TGATCA	1 cut(s)	744
Kzo9I	GATC	4 cut(s)	154, 289, 582, 744
LguI	GCTCTTC	1 cut(s)	641
LmnI	GCTCC	1 cut(s)	634
Lsp1109I	GCAGC	2 cut(s)	64, 649
MaeIII	GTNAC	3 cut(s)	330, 426, 824
MalI	GATC	4 cut(s)	156, 291, 584, 746
MboI	GATC	4 cut(s)	154, 289, 582, 744
MboII	GAAGA	6 cut(s)	78, 284, 611, 658, 664, 817
MfeI	CAATTG	2 cut(s)	95, 456
MflI	RGATCY	1 cut(s)	582
MluCI	AATT	4 cut(s)	75, 82, 95, 456
MmeI	TCCRAC	1 cut(s)	81
MseI	TTAA	3 cut(s)	80, 173, 835
MslI	CAYNNNNRTG	1 cut(s)	197
MspA1I	CMGCKG	1 cut(s)	640
MspI	CCGG	1 cut(s)	663
MunI	CAATTG	2 cut(s)	95, 456
MvnI	CGCG	1 cut(s)	393
NdeII	GATC	4 cut(s)	154, 289, 582, 744
NlaIII	CATG	5 cut(s)	48, 196, 403, 764, 774
NlaIV	GGNNCC	1 cut(s)	812
NmuCI	GTSAC	2 cut(s)	426, 824
NspI	RCATGY	2 cut(s)	48, 764
PagI	TCATGA	1 cut(s)	399
PciI	ACATGT	1 cut(s)	44
PciSI	GCTCTTC	1 cut(s)	641
PfeI	GAWTC	1 cut(s)	386
PflMI	CCANNNNNTGG	2 cut(s)	100, 137
PkrI	GCNGC	4 cut(s)	6, 54, 226, 639
PpuMI	RGGWCCY	1 cut(s)	811
PscI	ACATGT	1 cut(s)	44
Psp5II	RGGWCCY	1 cut(s)	811
PspEI	GGTNACC	1 cut(s)	824
PspFI	CCCAGC	2 cut(s)	445, 607
PspN4I	GGNNCC	1 cut(s)	812
PspPI	GGNCC	2 cut(s)	212, 811
PspPPI	RGGWCCY	1 cut(s)	811
PstNI	CAGNNNCTG	1 cut(s)	137
PsuI	RGATCY	1 cut(s)	582
PvuII	CAGCTG	1 cut(s)	640
RsaI	GTAC	3 cut(s)	221, 407, 656
RsaNI	GTAC	3 cut(s)	220, 406, 655
RseI	CAYNNNNRTG	1 cut(s)	197
SapI	GCTCTTC	1 cut(s)	641
SaqAI	TTAA	3 cut(s)	80, 173, 835
SatI	GCNGC	4 cut(s)	5, 53, 225, 638
Sau3AI	GATC	4 cut(s)	154, 289, 582, 744
Sau96I	GGNCC	2 cut(s)	212, 811
ScaI	AGTACT	1 cut(s)	656
SetI	ASST	7 cut(s)	139, 207, 267, 407, 609, 642, 699
SfcI	CTRYAG	1 cut(s)	815
SinI	GGWCC	1 cut(s)	811
SmiMI	CAYNNNNRTG	1 cut(s)	197
SmlI	CTYRAG	2 cut(s)	296, 326
SmoI	CTYRAG	2 cut(s)	296, 326
Sse9I	AATT	4 cut(s)	75, 82, 95, 456
SsiI	CCGC	1 cut(s)	225
TaaI	ACNGT	3 cut(s)	280, 349, 819
TaqI	TCGA	1 cut(s)	644
TasI	AATT	4 cut(s)	75, 82, 95, 456
TatI	WGTACW	1 cut(s)	654
TauI	GCSGC	1 cut(s)	227
TfiI	GAWTC	1 cut(s)	386
Tru1I	TTAA	3 cut(s)	80, 173, 835
Tru9I	TTAA	3 cut(s)	80, 173, 835
TseFI	GTSAC	2 cut(s)	426, 824
TseI	GCWGC	3 cut(s)	4, 52, 637
Tsp45I	GTSAC	2 cut(s)	426, 824
TspDTI	ATGAA	1 cut(s)	399
Van91I	CCANNNNNTGG	2 cut(s)	100, 137
VpaK11BI	GGWCC	1 cut(s)	811
XapI	RAATTY	1 cut(s)	82
XceI	RCATGY	2 cut(s)	48, 764
XcmI	CCANNNNNNNNNTGG	1 cut(s)	97
XmiI	GTMKAC	1 cut(s)	41
ZrmI	AGTACT	1 cut(s)	656

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.