Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0007278

Physical Location & Seq

Genomic Coordinates Reverse (-)

27256421 .. 27260340

Gene Structure 3920 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1224000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGCACTTCCCGCCATCGCAAGAAGACTGCGAAGCAAACATTCCCAGCTCTTCCTATCACACACCCTACTCCCATCCATTTCTTACTCACCGTCTTCGCCTTGTTCCTCACAACCTCTCCTCCGCGATCTCTGTCAGACATTTCATCGAACACTTATCGTGCTCCCTCAAACCCCTCGCCATTTCTCTACTCTCCAACCCTTCTCAGCCCAAACCCTCAACGACCCCTTGGGCCAACGATTCAACACAGACCATCCCCGCGACTCGCGTTTCACCCAATTTCTCGAATTGCTCAAACGCGCCGCCGATTTTGCCTCAGAAGCCGAGGCCGTGGCTTTTCTCGACGAGTCGGGCATCGAGGTGGATCGAGAAACGGTGCTTTTGGCGATTTGGGAGTTGAGAGAAGATTGGAAGTCGGCGTTTTTGGCTTTCCAATGGGGTGAGAAGTGGGGCTGTTGCAACGAAGAGGCTTGTAGTTTGATGGTATGGATTCTGGGGAGTCACAAAAAGTTCAGTACTGCTTGGTGTTTGATACGAGACTTGCATCGGGCTTTGATGGATACGCGGCGTGCAATGCTTATTATGATTGATAGATATGCATCTGCGAATGATCCGTGTAAGGCCATTAAGACATTCCATGTAATGGACAAGTTCAGATTGACTCCTGATCAAGAAGCATTCCACATCCTCTTGAATGCTCTCTGTAAATATGGAAACATCGAAGAAGCTGAAGAGTTCATGCTTGTAAATAAGAAGCTTTTCCCACTGGAGACCGAGAGCTTTAACATTATTCTGAATGGGTGGTGTAACATATCTGTTGATGTATTTGAAGCCAAGAGAGTTTGGAGAGAAATGTCAAAATGTTGTGTTACACCAGATGCAACTTCTTATACGCACTTGATCTCCTGCTTCTCAAAGGTTGGGAAACTATTTGACTCCCTTAGGCTCTATGATGAAATGAAGAAGAGGGGTTGGATCCCTGGGATCAGCGTTTATAACTCATTAATTTATGTTCTAACTTGTGAAAATTGTTTTAAAGAAGCTCTCAAAATATTGGACAAATTGAAAGAAGAGGGTTTGCAGGCAGATGCTACCACGTATAACTCAATGATATGTCCTTTATGTGAATCAGAAAAGCTAGAGGAGGCGAGGCAGATGTTGAGCGCTATGATTGCTAATAATCTCAGCCCGACCACTGAGACTTACAATGCGTTTCTCCAGAGCACAGGTTTAGAGGGAACTCTTGAAATTCTTAATCGGATGAAGAAAGCTAGTTTAGGTCCCAGTAGCAGTACCTTTCTTATGATTCTTGGAAAGTTCTTCAGATTAGAGCAACCTGAAATGGCATTGAAGATTTGGACGGAAATGAAGCAGTATGGAGTAGTGCCTGATTCTGCACATTACACAGTAATGGTACAAGGGCTGGCAGCTTGTCGGTTGTTAATTAAGGCCAAGGAAATATTTTCTGAGATGAAGACTAACGGATTTGTAGAAGATCCAAAGCTTAAGAGGCTTTTGGAGGTGCCAGGTAGTCGTGTTAAAGGAAAACGGAGACGGATACCAGTCAAGCAAGCAACAAAGGTTAATCAAAAGCAAGGTAGTACAAAGAGATGGAATAGTCTACGCAAATCAAGAAAGAAAAAGGCATCAATTAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

553

Amino Acids

63.2

Weight (kDa)

9.12

Isoelectric Point (pI)

49.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	260 - 306	3.9e-06	PPR repeat family
TPR_24	PF23276	280 - 385	5.8e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	281 - 338	5.4e-07	Pentatricopeptide repeat domain
PPR_long	PF17177	290 - 432	1.3e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	292 - 336	7.1e-08	PPR repeat family
PPR	PF01535	296 - 324	4.1e-06	PPR repeat
PPR_2	PF13041	327 - 375	2.4e-08	PPR repeat family
PPR_3	PF13812	350 - 409	1.9e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	363 - 407	4.1e-08	PPR repeat family
PPR_2	PF13041	428 - 476	9.7e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007278)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80880 AT1G80880 AT1G80880
fragaria_vesca	FvH4_6g28510 FvH4_6g28510
malus_domestica	MD17G1224000.v1.1
prunus_persica	Prupe.3G099700_v2.0.a1 Prupe.3G099700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0132781
rosa_laevigata	RLG00000019275 RLG00000019279 RLG00000019280 RLG00000019283
rosa_multiflora	Rmu_co8097930.1_g000001 Rmu_ssc0000074.1_g000001
rosa_roxburghii	Rroxscaffold_2G00111670
rosa_rugosa	Rorug02G0307400 Rorug02G0307500 Rorug02G0307600 Rorug02G0307700
rosa_samantha	Rh2AG360100 Rh2BG366000 Rh2CG343400 Rh2DG383000
rosa_wichuraiana	Rw2G029270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	996
AccB1I	GGYRCC	1 cut(s)	1522
AccB7I	CCANNNNNTGG	1 cut(s)	643
AccI	GTMKAC	1 cut(s)	1621
AccII	CGCG	5 cut(s)	126, 261, 268, 300, 565
AciI	CCGC	5 cut(s)	12, 124, 259, 303, 565
AclWI	GGATC	6 cut(s)	372, 605, 970, 983, 992, 1490
AcsI	RAATTY	1 cut(s)	1248
AcuI	CTGAAG	2 cut(s)	750, 1306
AfaI	GTAC	4 cut(s)	517, 1294, 1416, 1603
AfeI	AGCGCT	1 cut(s)	1165
AfiI	CCNNNNNNNGG	1 cut(s)	643
AflII	CTTAAG	1 cut(s)	1505
AgsI	TTSAA	6 cut(s)	244, 694, 830, 1066, 1247, 1351
AjnI	CCWGG	2 cut(s)	979, 1525
AluBI	AGCT	9 cut(s)	49, 728, 757, 780, 1043, 1138, 1271, 1430, 1504
AluI	AGCT	9 cut(s)	49, 728, 757, 780, 1043, 1138, 1271, 1430, 1504
Alw21I	GWGCWC	2 cut(s)	165, 1226
Alw26I	GTCTC	4 cut(s)	531, 764, 1193, 1546
AlwI	GGATC	6 cut(s)	372, 605, 970, 983, 992, 1490
Aor51HI	AGCGCT	1 cut(s)	1165
AoxI	GGCC	4 cut(s)	232, 327, 621, 1449
ApeKI	GCWGC	1 cut(s)	1427
ApoI	RAATTY	1 cut(s)	1248
AseI	ATTAAT	1 cut(s)	1004
Asp700I	GAANNNNTTC	1 cut(s)	758
AspLEI	GCGC	2 cut(s)	302, 1166
AspS9I	GGNCC	2 cut(s)	232, 1280
AsuHPI	GGTGA	3 cut(s)	81, 265, 452
AvaII	GGWCC	1 cut(s)	1280
AxyI	CCTNAGG	1 cut(s)	941
BamHI	GGATCC	1 cut(s)	975
BanI	GGYRCC	1 cut(s)	1522
BbsI	GAAGAC	3 cut(s)	31, 87, 1481
Bbv12I	GWGCWC	2 cut(s)	165, 1226
BbvI	GCAGC	1 cut(s)	1439
BccI	CCATC	6 cut(s)	23, 82, 261, 475, 550, 1605
BceAI	ACGGC	1 cut(s)	314
BciT130I	CCWGG	2 cut(s)	981, 1527
BciVI	GTATCC	2 cut(s)	553, 1551
BclI	TGATCA	1 cut(s)	667
BcoDI	GTCTC	4 cut(s)	531, 764, 1193, 1546
BfaI	CTAG	2 cut(s)	1139, 1272
BfoI	RGCGCY	1 cut(s)	1167
BfrI	CTTAAG	1 cut(s)	1505
BfuI	GTATCC	2 cut(s)	553, 1551
BisI	GCNGC	3 cut(s)	303, 566, 1428
BlsI	GCNGC	3 cut(s)	304, 567, 1429
BmcAI	AGTACT	1 cut(s)	517
Bme1390I	CCNGG	2 cut(s)	981, 1527
Bme18I	GGWCC	1 cut(s)	1280
BmgT120I	GGNCC	2 cut(s)	232, 1280
BmiI	GGNNCC	3 cut(s)	977, 1282, 1524
BmrFI	CCNGG	2 cut(s)	981, 1527
BmrI	ACTGGG	1 cut(s)	1278
BmsI	GCATC	6 cut(s)	363, 553, 608, 868, 1078, 1655
BmuI	ACTGGG	1 cut(s)	1278
BpiI	GAAGAC	3 cut(s)	31, 87, 1481
BpmI	CTGGAG	2 cut(s)	788, 1202
BsaAI	YACGTR	1 cut(s)	1098
BsaBI	GATNNNNATC	1 cut(s)	598
BsaI	GGTCTC	1 cut(s)	764
BsaJI	CCNNGG	6 cut(s)	228, 324, 330, 979, 980, 1452
BsaXI	ACNNNNNCTCC	6 cut(s)	102, 105, 132, 135, 887, 917
Bsc4I	CCNNNNNNNGG	1 cut(s)	643
Bse1I	ACTGG	3 cut(s)	771, 1284, 1562
Bse21I	CCTNAGG	1 cut(s)	941
Bse3DI	GCAATG	1 cut(s)	579
Bse8I	GATNNNNATC	1 cut(s)	598
BseBI	CCWGG	2 cut(s)	981, 1527
BseDI	CCNNGG	6 cut(s)	228, 324, 330, 979, 980, 1452
BseGI	GGATG	4 cut(s)	74, 253, 684, 1266
BseJI	GATNNNNATC	1 cut(s)	598
BseLI	CCNNNNNNNGG	1 cut(s)	643
BseMI	GCAATG	1 cut(s)	579
BseMII	CTCAG	5 cut(s)	219, 330, 1188, 1198, 1458
BseNI	ACTGG	3 cut(s)	771, 1284, 1562
BseRI	GAGGAG	2 cut(s)	110, 1157
BseXI	GCAGC	1 cut(s)	1439
BseYI	CCCAGC	1 cut(s)	45
BsgI	GTGCAG	1 cut(s)	1380
Bsh1236I	CGCG	5 cut(s)	126, 261, 268, 300, 565
BshFI	GGCC	4 cut(s)	234, 329, 623, 1451
BshNI	GGYRCC	1 cut(s)	1522
BsiHKAI	GWGCWC	2 cut(s)	165, 1226
BslFI	GGGAC	1 cut(s)	1266
BslI	CCNNNNNNNGG	1 cut(s)	643
BsmAI	GTCTC	4 cut(s)	531, 764, 1193, 1546
BsmBI	CGTCTC	1 cut(s)	1546
BsmFI	GGGAC	1 cut(s)	1266
BsmI	GAATGC	2 cut(s)	677, 700
BsnI	GGCC	4 cut(s)	234, 329, 623, 1451
Bso31I	GGTCTC	1 cut(s)	764
Bsp1286I	GDGCHC	2 cut(s)	165, 1226
Bsp143I	GATC	8 cut(s)	127, 364, 610, 667, 900, 975, 984, 1495
BspACI	CCGC	5 cut(s)	12, 124, 259, 303, 565
BspANI	GGCC	4 cut(s)	234, 329, 623, 1451
BspCNI	CTCAG	5 cut(s)	218, 329, 1189, 1197, 1459
BspFNI	CGCG	5 cut(s)	126, 261, 268, 300, 565
BspLI	GGNNCC	3 cut(s)	977, 1282, 1524
BspPI	GGATC	6 cut(s)	372, 605, 970, 983, 992, 1490
BspQI	GCTCTTC	1 cut(s)	56
BspT107I	GGYRCC	1 cut(s)	1522
BspTI	CTTAAG	1 cut(s)	1505
BspTNI	GGTCTC	1 cut(s)	764
BsrDI	GCAATG	1 cut(s)	579
BsrI	ACTGG	3 cut(s)	771, 1284, 1562
BssECI	CCNNGG	6 cut(s)	228, 324, 330, 979, 980, 1452
BssMI	GATC	8 cut(s)	127, 364, 610, 667, 900, 975, 984, 1495
BssT1I	CCWWGG	2 cut(s)	228, 1452
Bst2UI	CCWGG	2 cut(s)	981, 1527
Bst4CI	ACNGT	3 cut(s)	93, 376, 1408
Bst6I	CTCTTC	5 cut(s)	56, 459, 726, 959, 1065
BstAFI	CTTAAG	1 cut(s)	1505
BstBAI	YACGTR	1 cut(s)	1098
BstC8I	GCNNGC	4 cut(s)	570, 1083, 1425, 1572
BstDEI	CTNAG	6 cut(s)	205, 316, 941, 1184, 1197, 1467
BstDSI	CCRYGG	1 cut(s)	330
BstF5I	GGATG	4 cut(s)	74, 253, 684, 1266
BstFNI	CGCG	5 cut(s)	126, 261, 268, 300, 565
BstH2I	RGCGCY	1 cut(s)	1167
BstHHI	GCGC	2 cut(s)	302, 1166
BstKTI	GATC	8 cut(s)	130, 367, 613, 670, 903, 978, 987, 1498
BstMAI	GTCTC	4 cut(s)	531, 764, 1193, 1546
BstMBI	GATC	8 cut(s)	127, 364, 610, 667, 900, 975, 984, 1495
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 311, 320, 425, 574, 1172, 1510
BstNI	CCWGG	2 cut(s)	981, 1527
BstSCI	CCNGG	2 cut(s)	979, 1525
BstUI	CGCG	5 cut(s)	126, 261, 268, 300, 565
BstV1I	GCAGC	1 cut(s)	1439
BstV2I	GAAGAC	3 cut(s)	31, 87, 1481
BstX2I	RGATCY	2 cut(s)	975, 1495
BstYI	RGATCY	2 cut(s)	975, 1495
Bsu36I	CCTNAGG	1 cut(s)	941
BsuI	GTATCC	2 cut(s)	553, 1551
BsuRI	GGCC	4 cut(s)	234, 329, 623, 1451
BtgI	CCRYGG	1 cut(s)	330
BtsCI	GGATG	4 cut(s)	74, 253, 684, 1266
BtsIMutI	CAGTG	2 cut(s)	764, 1194
Cac8I	GCNNGC	4 cut(s)	570, 1083, 1425, 1572
CfoI	GCGC	2 cut(s)	302, 1166
Cfr13I	GGNCC	2 cut(s)	232, 1280
Csp6I	GTAC	4 cut(s)	516, 1293, 1415, 1602
CviAII	CATG	2 cut(s)	638, 739
CviQI	GTAC	4 cut(s)	516, 1293, 1415, 1602
DdeI	CTNAG	6 cut(s)	205, 316, 941, 1184, 1197, 1467
DpnI	GATC	8 cut(s)	129, 366, 612, 669, 902, 977, 986, 1497
DpnII	GATC	8 cut(s)	127, 364, 610, 667, 900, 975, 984, 1495
DraI	TTTAAA	1 cut(s)	1036
Eam1104I	CTCTTC	5 cut(s)	56, 459, 726, 959, 1065
EarI	CTCTTC	5 cut(s)	56, 459, 726, 959, 1065
Eco130I	CCWWGG	2 cut(s)	228, 1452
Eco31I	GGTCTC	1 cut(s)	764
Eco47I	GGWCC	1 cut(s)	1280
Eco47III	AGCGCT	1 cut(s)	1165
Eco57I	CTGAAG	2 cut(s)	750, 1306
Eco81I	CCTNAGG	1 cut(s)	941
EcoO109I	RGGNCCY	1 cut(s)	1280
EcoRII	CCWGG	2 cut(s)	979, 1525
EcoT14I	CCWWGG	2 cut(s)	228, 1452
EcoT22I	ATGCAT	1 cut(s)	601
ErhI	CCWWGG	2 cut(s)	228, 1452
Esp3I	CGTCTC	1 cut(s)	1546
FaeI	CATG	2 cut(s)	641, 742
FaqI	GGGAC	1 cut(s)	1266
FatI	CATG	2 cut(s)	637, 738
FauI	CCCGC	2 cut(s)	19, 266
FbaI	TGATCA	1 cut(s)	667
FblI	GTMKAC	1 cut(s)	1621
Fnu4HI	GCNGC	3 cut(s)	303, 566, 1428
FokI	GGATG	4 cut(s)	61, 240, 671, 1273
Fsp4HI	GCNGC	3 cut(s)	303, 566, 1428
FspBI	CTAG	2 cut(s)	1139, 1272
GlaI	GCGC	2 cut(s)	301, 1165
GluI	GCNGC	3 cut(s)	303, 566, 1428
GsaI	CCCAGC	1 cut(s)	49
GsuI	CTGGAG	2 cut(s)	788, 1202
HaeII	RGCGCY	1 cut(s)	1167
HaeIII	GGCC	4 cut(s)	234, 329, 623, 1451
HhaI	GCGC	2 cut(s)	302, 1166
Hin1II	CATG	2 cut(s)	641, 742
Hin6I	GCGC	2 cut(s)	300, 1164
HinP1I	GCGC	2 cut(s)	300, 1164
HindIII	AAGCTT	2 cut(s)	755, 1502
HphI	GGTGA	3 cut(s)	81, 265, 452
Hpy166II	GTNNAC	1 cut(s)	1622
Hpy188I	TCNGA	8 cut(s)	138, 319, 656, 795, 1132, 1260, 1325, 1468
Hpy188III	TCNNGA	9 cut(s)	284, 341, 368, 665, 671, 691, 1219, 1244, 1632
Hpy8I	GTNNAC	1 cut(s)	1622
Hpy99I	CGWCG	1 cut(s)	347
HpyAV	CCTTC	1 cut(s)	211
HpyCH4III	ACNGT	3 cut(s)	93, 376, 1408
HpyCH4IV	ACGT	1 cut(s)	1097
HpyCH4V	TGCA	7 cut(s)	459, 544, 572, 599, 881, 1081, 1397
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 311, 320, 425, 574, 1172, 1510
HpyF3I	CTNAG	6 cut(s)	205, 316, 941, 1184, 1197, 1467
HpySE526I	ACGT	1 cut(s)	1097
Hsp92II	CATG	2 cut(s)	641, 742
HspAI	GCGC	2 cut(s)	300, 1164
Ksp22I	TGATCA	1 cut(s)	667
Kzo9I	GATC	8 cut(s)	127, 364, 610, 667, 900, 975, 984, 1495
LguI	GCTCTTC	1 cut(s)	56
LmnI	GCTCC	1 cut(s)	168
Lsp1109I	GCAGC	1 cut(s)	1439
LweI	GCATC	6 cut(s)	363, 553, 608, 868, 1078, 1655
MaeI	CTAG	2 cut(s)	1139, 1272
MaeII	ACGT	1 cut(s)	1097
MaeIII	GTNAC	3 cut(s)	500, 806, 868
MalI	GATC	8 cut(s)	129, 366, 612, 669, 902, 977, 986, 1497
MboI	GATC	8 cut(s)	127, 364, 610, 667, 900, 975, 984, 1495
MflI	RGATCY	2 cut(s)	975, 1495
MhlI	GDGCHC	2 cut(s)	165, 1226
MluCI	AATT	8 cut(s)	278, 287, 1005, 1027, 1061, 1248, 1443, 1650
MlyI	GAGTC	5 cut(s)	257, 356, 508, 655, 929
MmeI	TCCRAC	2 cut(s)	220, 953
Mph1103I	ATGCAT	1 cut(s)	601
MroXI	GAANNNNTTC	1 cut(s)	758
MslI	CAYNNNNRTG	2 cut(s)	359, 1409
MspCI	CTTAAG	1 cut(s)	1505
MspR9I	CCNGG	2 cut(s)	981, 1527
Mva1269I	GAATGC	2 cut(s)	677, 700
MvaI	CCWGG	2 cut(s)	981, 1527
MvnI	CGCG	5 cut(s)	126, 261, 268, 300, 565
MwoI	GCNNNNNNNGC	7 cut(s)	11, 311, 320, 425, 574, 1172, 1510
NdeII	GATC	8 cut(s)	127, 364, 610, 667, 900, 975, 984, 1495
NlaIII	CATG	2 cut(s)	641, 742
NlaIV	GGNNCC	3 cut(s)	977, 1282, 1524
NmeAIII	GCCGAG	1 cut(s)	349
NmuCI	GTSAC	1 cut(s)	500
NsiI	ATGCAT	1 cut(s)	601
PacI	TTAATTAA	1 cut(s)	1446
PasI	CCCWGGG	1 cut(s)	980
PciSI	GCTCTTC	1 cut(s)	56
PctI	GAATGC	2 cut(s)	677, 700
PdmI	GAANNNNTTC	1 cut(s)	758
PfeI	GAWTC	5 cut(s)	240, 490, 1127, 1306, 1391
PflMI	CCANNNNNTGG	1 cut(s)	643
PkrI	GCNGC	3 cut(s)	304, 567, 1429
PleI	GAGTC	5 cut(s)	257, 355, 507, 655, 929
PpsI	GAGTC	5 cut(s)	257, 355, 507, 655, 929
Ppu21I	YACGTR	1 cut(s)	1098
PpuMI	RGGWCCY	1 cut(s)	1280
PshBI	ATTAAT	1 cut(s)	1004
PsiI	TTATAA	1 cut(s)	996
Psp5II	RGGWCCY	1 cut(s)	1280
Psp6I	CCWGG	2 cut(s)	979, 1525
PspFI	CCCAGC	1 cut(s)	45
PspGI	CCWGG	2 cut(s)	979, 1525
PspN4I	GGNNCC	3 cut(s)	977, 1282, 1524
PspPI	GGNCC	2 cut(s)	232, 1280
PspPPI	RGGWCCY	1 cut(s)	1280
PsuI	RGATCY	2 cut(s)	975, 1495
RsaI	GTAC	4 cut(s)	517, 1294, 1416, 1603
RsaNI	GTAC	4 cut(s)	516, 1293, 1415, 1602
RseI	CAYNNNNRTG	2 cut(s)	359, 1409
SapI	GCTCTTC	1 cut(s)	56
SatI	GCNGC	3 cut(s)	303, 566, 1428
Sau3AI	GATC	8 cut(s)	127, 364, 610, 667, 900, 975, 984, 1495
Sau96I	GGNCC	2 cut(s)	232, 1280
ScaI	AGTACT	1 cut(s)	517
SchI	GAGTC	5 cut(s)	257, 356, 508, 655, 929
ScrFI	CCNGG	2 cut(s)	981, 1527
SduI	GDGCHC	2 cut(s)	165, 1226
SfaNI	GCATC	6 cut(s)	363, 553, 608, 868, 1078, 1655
SinI	GGWCC	1 cut(s)	1280
SmiMI	CAYNNNNRTG	2 cut(s)	359, 1409
SmlI	CTYRAG	1 cut(s)	1505
SmoI	CTYRAG	1 cut(s)	1505
Sse9I	AATT	8 cut(s)	278, 287, 1005, 1027, 1061, 1248, 1443, 1650
SsiI	CCGC	5 cut(s)	12, 124, 259, 303, 565
SspI	AATATT	2 cut(s)	1053, 1461
SspMI	CTAG	2 cut(s)	1139, 1272
StyD4I	CCNGG	2 cut(s)	979, 1525
StyI	CCWWGG	2 cut(s)	228, 1452
TaaI	ACNGT	3 cut(s)	93, 376, 1408
TaiI	ACGT	1 cut(s)	1100
TaqI	TCGA	6 cut(s)	148, 285, 342, 357, 367, 720
TaqII	GACCGA	1 cut(s)	788
TasI	AATT	8 cut(s)	278, 287, 1005, 1027, 1061, 1248, 1443, 1650
TatI	WGTACW	2 cut(s)	515, 1601
TauI	GCSGC	2 cut(s)	305, 568
TfiI	GAWTC	5 cut(s)	240, 490, 1127, 1306, 1391
TscAI	CASTG	2 cut(s)	771, 1201
TseFI	GTSAC	1 cut(s)	500
TseI	GCWGC	1 cut(s)	1427
Tsp45I	GTSAC	1 cut(s)	500
TspDTI	ATGAA	7 cut(s)	134, 727, 969, 974, 1277, 1382, 1487
TspGWI	ACGGA	5 cut(s)	603, 1376, 1497, 1564, 1570
TspRI	CASTG	2 cut(s)	771, 1201
Van91I	CCANNNNNTGG	1 cut(s)	643
Vha464I	CTTAAG	1 cut(s)	1505
VpaK11BI	GGWCC	1 cut(s)	1280
VspI	ATTAAT	1 cut(s)	1004
XapI	RAATTY	1 cut(s)	1248
XmiI	GTMKAC	1 cut(s)	1621
XmnI	GAANNNNTTC	1 cut(s)	758
XspI	CTAG	2 cut(s)	1139, 1272
ZrmI	AGTACT	1 cut(s)	517
Zsp2I	ATGCAT	1 cut(s)	601

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.