Rh2AG360100

Pentatricopeptide repeat-containing protein At1g80880

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007278
Physical Location & Seq
Forward (+)
53353464 .. 53355594
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG360100.1

Sequence Viewer

Length: 1668 bp
ATGGCACTACCCTCCATAGCAAGAAGGCTACGAACTAAACACCCCCTCTGCCTATCACTCACTCTACTCCACCACCACCACCCCATCTCTACCTCACCCTATTCTTCACTTTCTCTCCATCCCCGTCTCTCTCGGCCATTTCCTCCAACATCTATCGTGCCATCCCAAAGCCCTCGCCATTTCTCTACTCTCCGACCCTTCTCCGCCGAAATCCCCCACCACCCATTGGGCTTCAATGGCCAGCGTTTCAACACAGACCACTCCCACAACTTGGGTCTCACCCAGTTTCTTGAATTGCTCACAAGTACCGCCGGTTTGGCCTCTGAAGCCGACGCCATGGCTTTTCTCGACGAGTCGGGCGTCGAGCCGAAGCGAGATACAGTCTTTCAGGCGATTTGGGAGCTGAGGGGAGATTGGAAGTTGGCGTTTTTGGGTTTCAAATGGGGTGAGAAGTGGGGCTGTTGCGATGAAGAGGCGTGTAGTTTGATGGTTTGGGTTTTCGGGAGTCACCGAAAGTTTAGTACTGCTTGGTGTTTGATACGGGACTTGAATCAGGCTTTGATTGATACGCGCCGCGCAATGCTTATAATGATTGATAGATATGCATCTGCAAATCATCCAAGTAAGGCCATTAAGACATTTCAGATAATGGAGAAGTTCAGGTTGAGCCCTGATCAAGAATCATTCCACATCCTCTTGAATGCACTTTGTAAAAATGGAAACATGGATGAAGCTGAAGAATTCATGCTTGTAAGTAAGAAGTTCTTCCCACTGGAGGTCGAGGGCTTTAACATTGTTCTTAATGGGTGGTGTAACATATCTGTGGATGCATATCAAGCCAAGAGAGTTTGGAGAGAAATGTCAAAATACTGTATTACACCAGACACAACTTCTTATACACACATGATTTCCTGCTTCTCAAAGGTTGGGAATCTTTTCGACTCCCTTAGACTATATGATGAAATGAAGAAAAGGGATTGGGTTCCTGGTCTCAAGGTTTACAATTCTTTAATTTATGTACTAACCCGTGAAAATTGCTTTAAGGAAGCACTCAAAGTACTGGACAAAGTGAAAGAAATTGGTTTGCAGCCAGATGCTACCACCTATAATTCAATGATAGGTCCTTTATGTGAATCAAAGAAGCTAGAGGAGGCGCAACAGATGCTGAGTTCTATGATAAATGATAACCTCAGCCCAACCATAGAGACTTACCATGCATTTCTGCAGAGTCCAGGTTCAAAGGCAACTGTTGAAGTTCTTAATCGAATGAAGAAAGCTAATTTAGGTCCTAATGGCAATACCTTCCTTATGATTCTTGAAAAGTTCTTTATATTAGAACAACCTGGGAAGGCATTGGAGATTTGGAATGAAATAAAGAACTATGGAGTAGTGCCTGATTCCACACATTACACCTCTATGGTACAGGGGCTGGCAAGTTGTGGGTTGTTAGCGAAGGCCAAGGAACTTTTGGCTGAGATGATGTCAAATGGCATTCCAGAAGATCCAAAGCTTAAGAAGCTCTTGAAGAAGCCAATTGGAGGTAGTGTTAAAGGGAAACGGAGAGTTAGACAAGTCAACCAAGCAAAAAGGATTAATCTAAAGCAAGACAGGGTTGTGAGATTGAGAAGTCCACGTCAGTCAAGAAAGAAGAAGACATCAAGTGAATAG

Protein Analysis

555

Amino Acids

63.16

Weight (kDa)

9.33

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 196 - 238 1.4e-07 PPR repeat family
PPR_1 PF12854 222 - 248 2.1e-06 PPR repeat
PPR_3 PF13812 282 - 340 3.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 294 - 329 9.8e-06 PPR repeat family
PPR_long PF17177 297 - 425 1.4e-07 Pentacotripeptide-repeat region of PRORP
PPR PF01535 298 - 325 9.9e-06 PPR repeat
PPR_2 PF13041 332 - 377 4.3e-09 PPR repeat family
PPR_3 PF13812 352 - 409 1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 361 - 392 8.7e-07 PPR repeat
PPR_2 PF13041 364 - 408 4e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AccB7I CCANNNNNTGG 2 cut(s) 226, 271
AccII CGCG 2 cut(s) 571, 576
AciI CCGC 3 cut(s) 204, 309, 574
AclWI GGATC 1 cut(s) 1496
AcoI YGGCCR 2 cut(s) 134, 238
AcsI RAATTY 1 cut(s) 740
AcuI CTGAAG 2 cut(s) 345, 756
AcyI GRCGYC 2 cut(s) 333, 360
AfaI GTAC 5 cut(s) 307, 523, 1020, 1059, 1422
AfiI CCNNNNNNNGG 4 cut(s) 226, 271, 775, 1538
AflII CTTAAG 1 cut(s) 1511
AjiI CACGTC 1 cut(s) 1634
AjnI CCWGG 3 cut(s) 985, 1231, 1342
AluBI AGCT 6 cut(s) 403, 734, 1144, 1277, 1510, 1519
AluI AGCT 6 cut(s) 403, 734, 1144, 1277, 1510, 1519
Alw26I GTCTC 4 cut(s) 131, 281, 995, 1199
AlwI GGATC 1 cut(s) 1496
AlwNI CAGNNNCTG 2 cut(s) 1165, 1429
AoxI GGCC 5 cut(s) 134, 238, 318, 627, 1455
ApeKI GCWGC 1 cut(s) 1087
ApoI RAATTY 1 cut(s) 740
AseI ATTAAT 1 cut(s) 1593
Asp700I GAANNNNTTC 2 cut(s) 764, 935
AspLEI GCGC 3 cut(s) 573, 578, 1156
AspS9I GGNCC 2 cut(s) 1121, 1286
AsuHPI GGTGA 4 cut(s) 87, 271, 458, 500
AvaII GGWCC 2 cut(s) 1121, 1286
BalI TGGCCA 1 cut(s) 240
BanII GRGCYC 1 cut(s) 671
BbsI GAAGAC 1 cut(s) 1658
BbvCI CCTCAGC 2 cut(s) 404, 1190
BbvI GCAGC 1 cut(s) 1099
BccI CCATC 4 cut(s) 92, 126, 169, 481
BciT130I CCWGG 3 cut(s) 987, 1233, 1344
BclI TGATCA 1 cut(s) 673
BcoDI GTCTC 4 cut(s) 131, 281, 995, 1199
BfaI CTAG 1 cut(s) 1145
BfmI CTRYAG 1 cut(s) 1223
BfrI CTTAAG 1 cut(s) 1511
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 2 cut(s) 574, 1088
BlsI GCNGC 2 cut(s) 575, 1089
BmcAI AGTACT 2 cut(s) 523, 1059
Bme1390I CCNGG 3 cut(s) 987, 1233, 1344
Bme18I GGWCC 2 cut(s) 1121, 1286
BmgBI CACGTC 1 cut(s) 1634
BmgT120I GGNCC 2 cut(s) 1121, 1286
BmiI GGNNCC 1 cut(s) 984
BmrFI CCNGG 3 cut(s) 987, 1233, 1344
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 4 cut(s) 614, 817, 1084, 1152
BmuI ACTGGG 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 1658
BpmI CTGGAG 1 cut(s) 794
Bpu10I CCTNAGC 2 cut(s) 404, 1190
BpuEI CTTGAG 1 cut(s) 977
BsaBI GATNNNNATC 2 cut(s) 604, 831
BsaHI GRCGYC 2 cut(s) 333, 360
BsaI GGTCTC 2 cut(s) 281, 995
BsaJI CCNNGG 3 cut(s) 336, 1343, 1458
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 1530, 1560
Bsc4I CCNNNNNNNGG 4 cut(s) 226, 271, 775, 1538
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 3 cut(s) 283, 777, 1065
Bse3DI GCAATG 1 cut(s) 585
Bse8I GATNNNNATC 2 cut(s) 604, 831
BseBI CCWGG 3 cut(s) 987, 1233, 1344
BseDI CCNNGG 3 cut(s) 336, 1343, 1458
BseGI GGATG 6 cut(s) 118, 161, 616, 690, 733, 832
BseJI GATNNNNATC 2 cut(s) 604, 831
BseLI CCNNNNNNNGG 4 cut(s) 226, 271, 775, 1538
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 4 cut(s) 395, 1157, 1204, 1464
BseNI ACTGG 3 cut(s) 283, 777, 1065
BseRI GAGGAG 1 cut(s) 1163
BseXI GCAGC 1 cut(s) 1099
Bsh1236I CGCG 2 cut(s) 571, 576
BshFI GGCC 5 cut(s) 136, 240, 320, 629, 1457
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 4 cut(s) 226, 271, 775, 1538
BsmAI GTCTC 4 cut(s) 131, 281, 995, 1199
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 557
BsmI GAATGC 2 cut(s) 706, 1491
BsnI GGCC 5 cut(s) 136, 240, 320, 629, 1457
Bso31I GGTCTC 2 cut(s) 281, 995
Bsp1286I GDGCHC 1 cut(s) 671
Bsp143I GATC 2 cut(s) 673, 1501
Bsp19I CCATGG 1 cut(s) 336
BspACI CCGC 3 cut(s) 204, 309, 574
BspANI GGCC 5 cut(s) 136, 240, 320, 629, 1457
BspCNI CTCAG 4 cut(s) 396, 1158, 1203, 1465
BspFNI CGCG 2 cut(s) 571, 576
BspLI GGNNCC 1 cut(s) 984
BspMAI CTGCAG 1 cut(s) 1227
BspPI GGATC 1 cut(s) 1496
BspTI CTTAAG 1 cut(s) 1511
BspTNI GGTCTC 2 cut(s) 281, 995
BsrDI GCAATG 1 cut(s) 585
BsrFI RCCGGY 1 cut(s) 311
BsrI ACTGG 3 cut(s) 283, 777, 1065
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 3 cut(s) 336, 1343, 1458
BssMI GATC 2 cut(s) 673, 1501
BssNI GRCGYC 2 cut(s) 333, 360
BssT1I CCWWGG 2 cut(s) 336, 1458
Bst2UI CCWGG 3 cut(s) 987, 1233, 1344
Bst4CI ACNGT 3 cut(s) 382, 872, 1249
Bst6I CTCTTC 1 cut(s) 465
BstACI GRCGYC 2 cut(s) 333, 360
BstAFI CTTAAG 1 cut(s) 1511
BstAPI GCANNNNNTGC 1 cut(s) 1162
BstC8I GCNNGC 2 cut(s) 242, 1431
BstDEI CTNAG 5 cut(s) 404, 947, 1166, 1190, 1473
BstDSI CCRYGG 1 cut(s) 336
BstF5I GGATG 6 cut(s) 118, 161, 616, 690, 733, 832
BstFNI CGCG 2 cut(s) 571, 576
BstHHI GCGC 3 cut(s) 573, 578, 1156
BstKTI GATC 2 cut(s) 676, 1504
BstMAI GTCTC 4 cut(s) 131, 281, 995, 1199
BstMBI GATC 2 cut(s) 673, 1501
BstMWI GCNNNNNNNGC 6 cut(s) 237, 317, 326, 836, 1162, 1516
BstNI CCWGG 3 cut(s) 987, 1233, 1344
BstSCI CCNGG 3 cut(s) 985, 1231, 1342
BstSFI CTRYAG 1 cut(s) 1223
BstUI CGCG 2 cut(s) 571, 576
BstV1I GCAGC 1 cut(s) 1099
BstV2I GAAGAC 1 cut(s) 1658
BstX2I RGATCY 1 cut(s) 1501
BstYI RGATCY 1 cut(s) 1501
BsuRI GGCC 5 cut(s) 136, 240, 320, 629, 1457
BtgI CCRYGG 1 cut(s) 336
BtgZI GCGATG 1 cut(s) 480
BtrI CACGTC 1 cut(s) 1634
BtsCI GGATG 6 cut(s) 118, 161, 616, 690, 733, 832
BtsIMutI CAGTG 1 cut(s) 770
Cac8I GCNNGC 2 cut(s) 242, 1431
CaiI CAGNNNCTG 2 cut(s) 1165, 1429
CfoI GCGC 3 cut(s) 573, 578, 1156
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 2 cut(s) 1121, 1286
CseI GACGC 2 cut(s) 341, 349
Csp6I GTAC 5 cut(s) 306, 522, 1019, 1058, 1421
CviAII CATG 5 cut(s) 337, 724, 745, 904, 1214
CviQI GTAC 5 cut(s) 306, 522, 1019, 1058, 1421
DdeI CTNAG 5 cut(s) 404, 947, 1166, 1190, 1473
DpnI GATC 2 cut(s) 675, 1503
DpnII GATC 2 cut(s) 673, 1501
EaeI YGGCCR 2 cut(s) 134, 238
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
EciI GGCGGA 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 336, 1458
Eco24I GRGCYC 1 cut(s) 671
Eco31I GGTCTC 2 cut(s) 281, 995
Eco47I GGWCC 2 cut(s) 1121, 1286
Eco57I CTGAAG 2 cut(s) 345, 756
EcoO109I RGGNCCY 2 cut(s) 1121, 1286
EcoRI GAATTC 1 cut(s) 740
EcoRII CCWGG 3 cut(s) 985, 1231, 1342
EcoT14I CCWWGG 2 cut(s) 336, 1458
EcoT22I ATGCAT 3 cut(s) 607, 832, 1219
EcoT38I GRGCYC 1 cut(s) 671
ErhI CCWWGG 2 cut(s) 336, 1458
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 5 cut(s) 340, 727, 748, 907, 1217
FalI AAGNNNNNCTT 4 cut(s) 749, 781, 1505, 1537
FaqI GGGAC 1 cut(s) 557
FatI CATG 5 cut(s) 336, 723, 744, 903, 1213
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 2 cut(s) 574, 1088
FokI GGATG 6 cut(s) 105, 148, 603, 677, 740, 839
FriOI GRGCYC 1 cut(s) 671
Fsp4HI GCNGC 2 cut(s) 574, 1088
FspBI CTAG 1 cut(s) 1145
GlaI GCGC 3 cut(s) 572, 577, 1155
GluI GCNGC 2 cut(s) 574, 1088
GsuI CTGGAG 1 cut(s) 794
HaeIII GGCC 5 cut(s) 136, 240, 320, 629, 1457
HapII CCGG 1 cut(s) 312
HgaI GACGC 2 cut(s) 341, 349
HhaI GCGC 3 cut(s) 573, 578, 1156
Hin1I GRCGYC 2 cut(s) 333, 360
Hin1II CATG 5 cut(s) 340, 727, 748, 907, 1217
Hin6I GCGC 3 cut(s) 571, 576, 1154
HinP1I GCGC 3 cut(s) 571, 576, 1154
HincII GTYRAC 1 cut(s) 1576
HindII GTYRAC 1 cut(s) 1576
HindIII AAGCTT 1 cut(s) 1508
HpaII CCGG 1 cut(s) 312
HphI GGTGA 4 cut(s) 87, 271, 458, 500
Hpy166II GTNNAC 3 cut(s) 1000, 1576, 1631
Hpy188I TCNGA 3 cut(s) 194, 325, 645
Hpy188III TCNNGA 9 cut(s) 290, 347, 502, 677, 697, 1316, 1496, 1522, 1641
Hpy8I GTNNAC 3 cut(s) 1000, 1576, 1631
Hpy99I CGWCG 3 cut(s) 335, 353, 365
HpyAV CCTTC 5 cut(s) 18, 208, 1312, 1342, 1447
HpyCH4III ACNGT 3 cut(s) 382, 872, 1249
HpyCH4IV ACGT 1 cut(s) 1633
HpyCH4V TGCA 7 cut(s) 605, 611, 704, 830, 1087, 1217, 1225
HpyF10VI GCNNNNNNNGC 6 cut(s) 237, 317, 326, 836, 1162, 1516
HpyF3I CTNAG 5 cut(s) 404, 947, 1166, 1190, 1473
HpySE526I ACGT 1 cut(s) 1633
Hsp92I GRCGYC 2 cut(s) 333, 360
Hsp92II CATG 5 cut(s) 340, 727, 748, 907, 1217
HspAI GCGC 3 cut(s) 571, 576, 1154
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 2 cut(s) 673, 1501
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 1 cut(s) 1099
LweI GCATC 4 cut(s) 614, 817, 1084, 1152
MaeI CTAG 1 cut(s) 1145
MaeII ACGT 1 cut(s) 1633
MaeIII GTNAC 2 cut(s) 506, 812
MalI GATC 2 cut(s) 675, 1503
MboI GATC 2 cut(s) 673, 1501
MfeI CAATTG 1 cut(s) 1533
MflI RGATCY 1 cut(s) 1501
MhlI GDGCHC 1 cut(s) 671
MlsI TGGCCA 1 cut(s) 240
MluCI AATT 9 cut(s) 293, 740, 1003, 1011, 1033, 1077, 1108, 1279, 1533
MluNI TGGCCA 1 cut(s) 240
MlyI GAGTC 4 cut(s) 362, 514, 935, 1237
MmeI TCCRAC 2 cut(s) 170, 217
Mox20I TGGCCA 1 cut(s) 240
Mph1103I ATGCAT 3 cut(s) 607, 832, 1219
MroXI GAANNNNTTC 2 cut(s) 764, 935
MscI TGGCCA 1 cut(s) 240
MseI TTAA 9 cut(s) 633, 789, 801, 1010, 1041, 1260, 1512, 1548, 1593
MslI CAYNNNNRTG 2 cut(s) 821, 1415
Msp20I TGGCCA 1 cut(s) 240
MspCI CTTAAG 1 cut(s) 1511
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 3 cut(s) 987, 1233, 1344
MteI GCGCNGCGC 1 cut(s) 574
MunI CAATTG 1 cut(s) 1533
Mva1269I GAATGC 2 cut(s) 706, 1491
MvaI CCWGG 3 cut(s) 987, 1233, 1344
MvnI CGCG 2 cut(s) 571, 576
MwoI GCNNNNNNNGC 6 cut(s) 237, 317, 326, 836, 1162, 1516
NcoI CCATGG 1 cut(s) 336
NdeII GATC 2 cut(s) 673, 1501
NlaIII CATG 5 cut(s) 340, 727, 748, 907, 1217
NlaIV GGNNCC 1 cut(s) 984
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 506
NsiI ATGCAT 3 cut(s) 607, 832, 1219
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PctI GAATGC 2 cut(s) 706, 1491
PdmI GAANNNNTTC 2 cut(s) 764, 935
PfeI GAWTC 6 cut(s) 550, 680, 931, 1133, 1312, 1397
PflMI CCANNNNNTGG 2 cut(s) 226, 271
PkrI GCNGC 2 cut(s) 575, 1089
PleI GAGTC 4 cut(s) 361, 513, 935, 1236
PpsI GAGTC 4 cut(s) 361, 513, 935, 1236
PpuMI RGGWCCY 2 cut(s) 1121, 1286
PshBI ATTAAT 1 cut(s) 1593
PsiI TTATAA 1 cut(s) 587
Psp5II RGGWCCY 2 cut(s) 1121, 1286
Psp6I CCWGG 3 cut(s) 985, 1231, 1342
PspGI CCWGG 3 cut(s) 985, 1231, 1342
PspN4I GGNNCC 1 cut(s) 984
PspPI GGNCC 2 cut(s) 1121, 1286
PspPPI RGGWCCY 2 cut(s) 1121, 1286
PstI CTGCAG 1 cut(s) 1227
PstNI CAGNNNCTG 2 cut(s) 1165, 1429
PsuI RGATCY 1 cut(s) 1501
RsaI GTAC 5 cut(s) 307, 523, 1020, 1059, 1422
RsaNI GTAC 5 cut(s) 306, 522, 1019, 1058, 1421
RseI CAYNNNNRTG 2 cut(s) 821, 1415
SaqAI TTAA 9 cut(s) 633, 789, 801, 1010, 1041, 1260, 1512, 1548, 1593
SatI GCNGC 2 cut(s) 574, 1088
Sau3AI GATC 2 cut(s) 673, 1501
Sau96I GGNCC 2 cut(s) 1121, 1286
ScaI AGTACT 2 cut(s) 523, 1059
SchI GAGTC 4 cut(s) 362, 514, 935, 1237
ScrFI CCNGG 3 cut(s) 987, 1233, 1344
SduI GDGCHC 1 cut(s) 671
SfaNI GCATC 4 cut(s) 614, 817, 1084, 1152
SfcI CTRYAG 1 cut(s) 1223
SinI GGWCC 2 cut(s) 1121, 1286
SmiMI CAYNNNNRTG 2 cut(s) 821, 1415
SmlI CTYRAG 2 cut(s) 992, 1511
SmoI CTYRAG 2 cut(s) 992, 1511
Sse9I AATT 9 cut(s) 293, 740, 1003, 1011, 1033, 1077, 1108, 1279, 1533
SsiI CCGC 3 cut(s) 204, 309, 574
SspMI CTAG 1 cut(s) 1145
StyD4I CCNGG 3 cut(s) 985, 1231, 1342
StyI CCWWGG 2 cut(s) 336, 1458
TaaI ACNGT 3 cut(s) 382, 872, 1249
TaiI ACGT 1 cut(s) 1636
TaqI TCGA 5 cut(s) 348, 363, 780, 939, 1264
TasI AATT 9 cut(s) 293, 740, 1003, 1011, 1033, 1077, 1108, 1279, 1533
TatI WGTACW 3 cut(s) 521, 1018, 1057
TauI GCSGC 1 cut(s) 576
TfiI GAWTC 6 cut(s) 550, 680, 931, 1133, 1312, 1397
Tru1I TTAA 9 cut(s) 633, 789, 801, 1010, 1041, 1260, 1512, 1548, 1593
Tru9I TTAA 9 cut(s) 633, 789, 801, 1010, 1041, 1260, 1512, 1548, 1593
TscAI CASTG 1 cut(s) 777
TseFI GTSAC 1 cut(s) 506
TseI GCWGC 1 cut(s) 1087
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 7 cut(s) 483, 733, 744, 975, 980, 1283, 1383
TspGWI ACGGA 1 cut(s) 1573
TspRI CASTG 1 cut(s) 777
Van91I CCANNNNNTGG 2 cut(s) 226, 271
Vha464I CTTAAG 1 cut(s) 1511
VpaK11BI GGWCC 2 cut(s) 1121, 1286
VspI ATTAAT 1 cut(s) 1593
XapI RAATTY 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 1465
XmnI GAANNNNTTC 2 cut(s) 764, 935
XspI CTAG 1 cut(s) 1145
ZrmI AGTACT 2 cut(s) 523, 1059
Zsp2I ATGCAT 3 cut(s) 607, 832, 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.