RLG00000019275

Pentatricopeptide repeat-containing protein At1g80880

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007278
Physical Location & Seq
Forward (+)
49509722 .. 49510992
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019275

Sequence Viewer

Length: 795 bp
ATGCTTATAATGATTGATAGATATGCATCTGCAAATCATCCAAGTAAGGCCATTAAGACATTTCAGACAATGGAGAAGTTCAGGTTGAGCCCTGATCAAGAAGCATTCCACATCCTCTTGAATGCACTTTGTAAAAATGGAAACATAGAGGAAGCTGAAGAATTCATGCTTTTAAGTAAGAAGTTCTTCCCACTGGAGGTCGAGGGCTTTAACATTGTTCTCAATGGGTGGTGTAACATATCTGTGGATGTATATCAAGCCAAGAGAGTTTGGAGACAAATGTCAAAATACTGTATTACACCAGATGCAACTTCTTATACACACATGATTTCCTGCTTCTCAAATGTTGGGAATCTTTTTTATTCCCTTAGACTATATGATGAAATGAAGAAAAGGGATTGGATTCCTGGTCTCAAGGTTTACAATTCCTTAATTTATGTACTAACCTGTGAAAATTGCTTTAAGGAAGCACTCAAAGTACTGGACAAAGTGAAAGAAACTGGTTTGCAGCCAGATGCTACCACCTATAACTCAATGATAGGTCCTTTATGTGAATCAAAGAAGCTAGAGGAGGCGCAACAGATGCTGAGTTCTATGATAAATGATAACCTCAGCCCAACCATAGAGACTTACCATGGATTTCTCCAGAGTCCAGGTTCAAAGGCAACTCTTGAAATTCTTAATCGAATGAAGAAAGCTAATTTAGGTCCTAACGGCAATACCTTCCTTATGATTCTTGAAAAGTTTTTTATATTAGAACAACCTGGGAAGGCATCGGAGATTTGGAATAAATGA

Protein Analysis

265

Amino Acids

30.31

Weight (kDa)

7.54

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 3 - 45 1e-07 PPR repeat family
PPR_2 PF13041 69 - 114 6.2e-06 PPR repeat family
PPR_3 PF13812 90 - 147 6e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 101 - 145 8.5e-06 PPR repeat family
PPR_long PF17177 105 - 217 5.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 139 - 184 6.5e-09 PPR repeat family
PPR_3 PF13812 159 - 212 5.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 168 - 199 3e-07 PPR repeat
PPR_2 PF13041 174 - 212 3.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 8
AcsI RAATTY 2 cut(s) 161, 675
AcuI CTGAAG 1 cut(s) 177
AfaI GTAC 2 cut(s) 441, 480
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 4 cut(s) 121, 660, 674, 740
AjnI CCWGG 3 cut(s) 406, 652, 763
AluBI AGCT 3 cut(s) 155, 565, 698
AluI AGCT 3 cut(s) 155, 565, 698
Alw26I GTCTC 3 cut(s) 268, 416, 620
AlwNI CAGNNNCTG 1 cut(s) 586
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 508
ApoI RAATTY 2 cut(s) 161, 675
Asp700I GAANNNNTTC 1 cut(s) 185
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 2 cut(s) 542, 707
AvaII GGWCC 2 cut(s) 542, 707
BanII GRGCYC 1 cut(s) 92
BbvCI CCTCAGC 1 cut(s) 611
BbvI GCAGC 1 cut(s) 520
BceAI ACGGC 1 cut(s) 730
BciT130I CCWGG 3 cut(s) 408, 654, 765
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 3 cut(s) 268, 416, 620
BfaI CTAG 1 cut(s) 566
BisI GCNGC 1 cut(s) 509
BlsI GCNGC 1 cut(s) 510
BmcAI AGTACT 1 cut(s) 480
Bme1390I CCNGG 3 cut(s) 408, 654, 765
Bme18I GGWCC 2 cut(s) 542, 707
BmgT120I GGNCC 2 cut(s) 542, 707
BmrFI CCNGG 3 cut(s) 408, 654, 765
BmsI GCATC 5 cut(s) 35, 295, 505, 573, 782
BoxI GACNNNNGTC 1 cut(s) 280
BpmI CTGGAG 2 cut(s) 215, 629
Bpu10I CCTNAGC 1 cut(s) 611
BpuEI CTTGAG 1 cut(s) 398
BsaBI GATNNNNATC 2 cut(s) 25, 252
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 634, 764
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 3 cut(s) 198, 486, 505
Bse8I GATNNNNATC 2 cut(s) 25, 252
BseBI CCWGG 3 cut(s) 408, 654, 765
BseDI CCNNGG 2 cut(s) 634, 764
BseGI GGATG 3 cut(s) 37, 111, 253
BseJI GATNNNNATC 2 cut(s) 25, 252
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 2 cut(s) 578, 625
BseNI ACTGG 3 cut(s) 198, 486, 505
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 1 cut(s) 520
BshFI GGCC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 3 cut(s) 268, 416, 620
BsmI GAATGC 2 cut(s) 104, 127
BsnI GGCC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 1 cut(s) 94
Bsp19I CCATGG 1 cut(s) 634
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 2 cut(s) 579, 624
BspTNI GGTCTC 1 cut(s) 416
BsrI ACTGG 3 cut(s) 198, 486, 505
BssECI CCNNGG 2 cut(s) 634, 764
BssMI GATC 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 634
Bst2UI CCWGG 3 cut(s) 408, 654, 765
Bst4CI ACNGT 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 583
BstDEI CTNAG 3 cut(s) 368, 587, 611
BstDSI CCRYGG 1 cut(s) 634
BstF5I GGATG 3 cut(s) 37, 111, 253
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 3 cut(s) 268, 416, 620
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 1 cut(s) 583
BstNI CCWGG 3 cut(s) 408, 654, 765
BstPAI GACNNNNGTC 1 cut(s) 280
BstSCI CCNGG 3 cut(s) 406, 652, 763
BstV1I GCAGC 1 cut(s) 520
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 634
BtsCI GGATG 3 cut(s) 37, 111, 253
BtsIMutI CAGTG 1 cut(s) 191
CaiI CAGNNNCTG 1 cut(s) 586
CfoI GCGC 1 cut(s) 577
Cfr13I GGNCC 2 cut(s) 542, 707
Csp6I GTAC 2 cut(s) 440, 479
CviAII CATG 3 cut(s) 166, 325, 635
CviJI RGCY 9 cut(s) 50, 90, 155, 207, 260, 511, 565, 615, 698
CviKI_1 RGCY 9 cut(s) 50, 90, 155, 207, 260, 511, 565, 615, 698
CviQI GTAC 2 cut(s) 440, 479
DdeI CTNAG 3 cut(s) 368, 587, 611
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 634
Eco24I GRGCYC 1 cut(s) 92
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 2 cut(s) 542, 707
Eco57I CTGAAG 1 cut(s) 177
EcoO109I RGGNCCY 2 cut(s) 542, 707
EcoRI GAATTC 1 cut(s) 161
EcoRII CCWGG 3 cut(s) 406, 652, 763
EcoT14I CCWWGG 1 cut(s) 634
EcoT22I ATGCAT 1 cut(s) 28
EcoT38I GRGCYC 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 634
FaeI CATG 3 cut(s) 169, 328, 638
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FatI CATG 3 cut(s) 165, 324, 634
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 1 cut(s) 509
FokI GGATG 3 cut(s) 24, 98, 260
FriOI GRGCYC 1 cut(s) 92
Fsp4HI GCNGC 1 cut(s) 509
FspBI CTAG 1 cut(s) 566
GlaI GCGC 1 cut(s) 576
GluI GCNGC 1 cut(s) 509
GsuI CTGGAG 2 cut(s) 215, 629
HaeIII GGCC 1 cut(s) 50
HhaI GCGC 1 cut(s) 577
Hin1II CATG 3 cut(s) 169, 328, 638
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HinfI GANTC 5 cut(s) 352, 403, 554, 649, 733
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 2 cut(s) 66, 778
Hpy188III TCNNGA 5 cut(s) 98, 118, 646, 671, 737
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 2 cut(s) 733, 763
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4V TGCA 5 cut(s) 26, 32, 125, 308, 508
HpyF10VI GCNNNNNNNGC 1 cut(s) 583
HpyF3I CTNAG 3 cut(s) 368, 587, 611
Hsp92II CATG 3 cut(s) 169, 328, 638
HspAI GCGC 1 cut(s) 575
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 1 cut(s) 94
Lsp1109I GCAGC 1 cut(s) 520
LweI GCATC 5 cut(s) 35, 295, 505, 573, 782
MaeI CTAG 1 cut(s) 566
MaeIII GTNAC 1 cut(s) 233
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MboII GAAGA 4 cut(s) 170, 178, 400, 703
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 6 cut(s) 161, 424, 432, 454, 675, 700
MlyI GAGTC 1 cut(s) 658
MnlI CCTC 7 cut(s) 125, 142, 190, 196, 562, 565, 620
Mph1103I ATGCAT 1 cut(s) 28
MroXI GAANNNNTTC 1 cut(s) 185
MseI TTAA 6 cut(s) 54, 173, 210, 431, 462, 681
MslI CAYNNNNRTG 1 cut(s) 242
MspR9I CCNGG 3 cut(s) 408, 654, 765
Mva1269I GAATGC 2 cut(s) 104, 127
MvaI CCWGG 3 cut(s) 408, 654, 765
MwoI GCNNNNNNNGC 1 cut(s) 583
NcoI CCATGG 1 cut(s) 634
NdeII GATC 1 cut(s) 94
NlaIII CATG 3 cut(s) 169, 328, 638
NsiI ATGCAT 1 cut(s) 28
PctI GAATGC 2 cut(s) 104, 127
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 4 cut(s) 352, 403, 554, 733
PkrI GCNGC 1 cut(s) 510
PleI GAGTC 1 cut(s) 657
PpsI GAGTC 1 cut(s) 657
PpuMI RGGWCCY 2 cut(s) 542, 707
PshAI GACNNNNGTC 1 cut(s) 280
PsiI TTATAA 1 cut(s) 8
Psp5II RGGWCCY 2 cut(s) 542, 707
Psp6I CCWGG 3 cut(s) 406, 652, 763
PspGI CCWGG 3 cut(s) 406, 652, 763
PspPI GGNCC 2 cut(s) 542, 707
PspPPI RGGWCCY 2 cut(s) 542, 707
PstNI CAGNNNCTG 1 cut(s) 586
RsaI GTAC 2 cut(s) 441, 480
RsaNI GTAC 2 cut(s) 440, 479
RseI CAYNNNNRTG 1 cut(s) 242
SaqAI TTAA 6 cut(s) 54, 173, 210, 431, 462, 681
SatI GCNGC 1 cut(s) 509
Sau3AI GATC 1 cut(s) 94
Sau96I GGNCC 2 cut(s) 542, 707
ScaI AGTACT 1 cut(s) 480
SchI GAGTC 1 cut(s) 658
ScrFI CCNGG 3 cut(s) 408, 654, 765
SduI GDGCHC 1 cut(s) 92
SfaNI GCATC 5 cut(s) 35, 295, 505, 573, 782
SinI GGWCC 2 cut(s) 542, 707
SmiMI CAYNNNNRTG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 6 cut(s) 161, 424, 432, 454, 675, 700
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 3 cut(s) 406, 652, 763
StyI CCWWGG 1 cut(s) 634
TaaI ACNGT 1 cut(s) 293
TaqI TCGA 2 cut(s) 201, 685
TasI AATT 6 cut(s) 161, 424, 432, 454, 675, 700
TatI WGTACW 2 cut(s) 439, 478
TfiI GAWTC 4 cut(s) 352, 403, 554, 733
Tru1I TTAA 6 cut(s) 54, 173, 210, 431, 462, 681
Tru9I TTAA 6 cut(s) 54, 173, 210, 431, 462, 681
TscAI CASTG 1 cut(s) 198
TseI GCWGC 1 cut(s) 508
TspDTI ATGAA 4 cut(s) 154, 396, 401, 704
TspRI CASTG 1 cut(s) 198
VpaK11BI GGWCC 2 cut(s) 542, 707
XapI RAATTY 2 cut(s) 161, 675
XmnI GAANNNNTTC 1 cut(s) 185
XspI CTAG 1 cut(s) 566
ZrmI AGTACT 1 cut(s) 480
Zsp2I ATGCAT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.