Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0007158

Physical Location & Seq

Genomic Coordinates Forward (+)

29546511 .. 29552838

Gene Structure 6328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1246900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGGGAGCCAAGCTTGCCGGTCTTAGAATGGGTTCTGATGGGATTTCCATGTCCACTTTGAAACCCAGATCAGTCCACGCTCAGGCTTCTGATGGGGATGTTGAGAATCTTATTCCCTCCAAGCCTCAGGGGAAGTCCTCTGGAACAGTATTGCCGTATGTCGGTGTTGCTTGTCTTGGAGCCATTTTGTTTGGCTATCACCTGGGGGTGGTAAATGGTGCTCTTGAGTACCTATCGAAGGATCTTGGTATTGCGGAAAATGCGGTATTGCAAGGGTGGGTGGTTAGCACACTTCTAGCAGGTGCCACAGTTGGATCATTTACTGGTGGATCGTTGGCTGACAAGTTTGGCAGGACTAAGACTTTTCAAATAAATGCAATTCCGCTAGCAGTTGGAGCATTTTTGTGTGCAACAGCCCAGAGTGTGCAAACGATGATAGTTGGCCGCTTACTTGCTGGCATTGGAATTGGCGTTACTTCTGCTATTGTCCCACTTTATATATCTGAGATCTCTCCAACTGAAATTCGTGGTGCCCTTGGATCTGTAAACCAGCTTTTTATATGTATTGGGATTCTTGCAGCACTGGTGGCTGGATTACCATTAGCCGCAAATCCCTTATGGTGGAGGACGATGTTCGGTATTGCAGTAGTACCATCTGTTCTATTGGCATTAGGAATGGCTGTTTCACCAGAAAGTCCAAGATGGCTTTTTCAGCAAGGAAAAATTTCTGAAGCTGAAAAGGCTATAAAAACACTATTCGGAAAAGAAAGAGTTACTGAGGTCATGCATGACTTGAGATCAGCAACTTCAGGTTCTGCTGAACCTGAAGCGGGCTGGTTTGATTTGTTTAGTAGCCGCTATTGGAAAGTCGTTAGTGTGGGTGCAGCACTTTTCTTGTTACAACAGATGGCTGGGATAAATGCTGTGGTCTATTATTCTACTTCTGTCTTCCGCAGTGCTGGCATTACATCTGATGTTGCAGCAAGTGCTCTGGTTGGATTAGCAAATGTCTTGGGCACAGCTGTTGCATCCTCGTTGATGGATAAACAGGGAAGGAAAAGTCTTCTACTCACAAGCTTTGGTGGAATGGCTGCTTCGATGTTGCTGCTTTCACTGTCCTTTACATGGAAAGCCCTTGCTCCATATTCTGCCCCCCTTTCTGTCGCTGGAACTGTTCTCTATGTATTGTCCTTTTCACTTGGCGCTGGCCCTGTGCCTGCTCTTCTTCTACCAGAGATTTTTGCTTCAAGAATCAGAGCAAAAGCAGTTTCTTTGTCACTGGGCATGCACTGGATTTCAAACTTCGTCATAGGCCTCTATTTCTTGAGCCTTGTAACTAAGTTCGGGATCGGCACAGTGTATTTTGGATTTGCTGTCGTCTGTCTTCTGGCTGTCTTGTACATAGCTGGTAATGTTGTTGAAACGAAAGGTCGTTCGTTGGAGGAAATAGAGCGTGCTCTTAGTGTTGTCACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0022804 GO:0022857 GO:0022890 GO:0031967 GO:0031975 GO:0034219 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098655 GO:0098660 GO:0098662 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

492

Amino Acids

51.24

Weight (kDa)

9.24

Isoelectric Point (pI)

33.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	56 - 486	1e-106	Sugar (and other) transporter
MFS_1	PF07690	58 - 399	6.3e-28	Major Facilitator Superfamily
MFS_1	PF07690	329 - 468	8.1e-06	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007158)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16150 AT5G16150 AT5G16150
fragaria_vesca	FvH4_1g25970 FvH4_1g25970 FvH4_1g25970
malus_domestica	MD09G1254500.v1.1 MD17G1246900.v1.1
prunus_persica	Prupe.3G144600_v2.0.a1
pyrus_communis	pycom09g17020 pycom17g24850
rosa_chinensis	RchiOBHm_Chr3g0485241
rosa_laevigata	RLG00000023163
rosa_multiflora	Rmu_sc0005072.1_g000021 Rmu_sc0024791.1_g000001 Rmu_sc0038513.1_g000001
rosa_roxburghii	Rroxscaffold_6G00397150
rosa_rugosa	Rorug03G0217000 Rorug03G0217100
rosa_samantha	Rh3AG266500 Rh3BG302200 Rh3CG300200 Rh3DG296400
rosa_wichuraiana	Rw0G000470 Rw3G023760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	290
Acc36I	ACCTGC	1 cut(s)	290
AccB1I	GGYRCC	2 cut(s)	302, 530
AciI	CCGC	8 cut(s)	254, 263, 383, 445, 606, 830, 856, 952
AclWI	GGATC	5 cut(s)	249, 322, 337, 547, 1355
AcoI	YGGCCR	1 cut(s)	442
AcsI	RAATTY	2 cut(s)	522, 723
AcuI	CTGAAG	3 cut(s)	750, 792, 846
AfaI	GTAC	3 cut(s)	230, 651, 1400
AfiI	CCNNNNNNNGG	7 cut(s)	82, 161, 208, 238, 621, 830, 1125
AgsI	TTSAA	5 cut(s)	61, 368, 1248, 1299, 1421
AjnI	CCWGG	1 cut(s)	201
AluBI	AGCT	6 cut(s)	13, 553, 734, 1022, 1077, 1406
AluI	AGCT	6 cut(s)	13, 553, 734, 1022, 1077, 1406
Alw21I	GWGCWC	3 cut(s)	223, 991, 1459
AlwI	GGATC	5 cut(s)	249, 322, 337, 547, 1355
AlwNI	CAGNNNCTG	1 cut(s)	815
AoxI	GGCC	3 cut(s)	442, 1207, 1312
ApeKI	GCWGC	5 cut(s)	578, 884, 980, 1091, 1105
ApoI	RAATTY	2 cut(s)	522, 723
Asp700I	GAANNNNTTC	2 cut(s)	31, 724
AspLEI	GCGC	1 cut(s)	1205
AspS9I	GGNCC	1 cut(s)	1208
AsuHPI	GGTGA	2 cut(s)	191, 678
AsuNHI	GCTAGC	1 cut(s)	385
AxyI	CCTNAGG	1 cut(s)	126
BaeGI	GKGCMC	2 cut(s)	535, 1019
BanI	GGYRCC	2 cut(s)	302, 530
BbsI	GAAGAC	3 cut(s)	940, 1055, 1376
Bbv12I	GWGCWC	3 cut(s)	223, 991, 1459
BbvI	GCAGC	5 cut(s)	590, 896, 992, 1078, 1092
BccI	CCATC	6 cut(s)	32, 86, 661, 696, 901, 1033
BceAI	ACGGC	1 cut(s)	139
BcgI	CGANNNNNNTGC	2 cut(s)	1087, 1121
BciT130I	CCWGG	1 cut(s)	203
BfaI	CTAG	2 cut(s)	296, 386
BfoI	RGCGCY	1 cut(s)	1206
BfuAI	ACCTGC	1 cut(s)	290
BglII	AGATCT	1 cut(s)	507
BisI	GCNGC	8 cut(s)	445, 579, 606, 856, 885, 981, 1092, 1106
BlsI	GCNGC	8 cut(s)	446, 580, 607, 857, 886, 982, 1093, 1107
Bme1390I	CCNGG	1 cut(s)	203
BmgT120I	GGNCC	1 cut(s)	1208
BmiI	GGNNCC	4 cut(s)	7, 181, 304, 532
BmrFI	CCNGG	1 cut(s)	203
BmrI	ACTGGG	1 cut(s)	1289
BmsI	GCATC	1 cut(s)	1037
BmtI	GCTAGC	1 cut(s)	389
BmuI	ACTGGG	1 cut(s)	1289
BpiI	GAAGAC	3 cut(s)	940, 1055, 1376
Bpu10I	CCTNAGC	1 cut(s)	81
BpuEI	CTTGAG	3 cut(s)	245, 814, 1345
BsaJI	CCNNGG	2 cut(s)	202, 535
Bsc4I	CCNNNNNNNGG	7 cut(s)	82, 161, 208, 238, 621, 830, 1125
Bse118I	RCCGGY	1 cut(s)	17
Bse1I	ACTGG	4 cut(s)	328, 588, 1284, 1295
Bse21I	CCTNAGG	1 cut(s)	126
BseBI	CCWGG	1 cut(s)	203
BseDI	CCNNGG	2 cut(s)	202, 535
BseGI	GGATG	2 cut(s)	103, 1028
BseLI	CCNNNNNNNGG	7 cut(s)	82, 161, 208, 238, 621, 830, 1125
BseMII	CTCAG	4 cut(s)	95, 140, 495, 768
BseNI	ACTGG	4 cut(s)	328, 588, 1284, 1295
BseSI	GKGCMC	2 cut(s)	535, 1019
BseXI	GCAGC	5 cut(s)	590, 896, 992, 1078, 1092
BseYI	CCCAGC	1 cut(s)	911
BsgI	GTGCAG	1 cut(s)	903
BshFI	GGCC	3 cut(s)	444, 1209, 1314
BshNI	GGYRCC	2 cut(s)	302, 530
BsiHKAI	GWGCWC	3 cut(s)	223, 991, 1459
BsiSI	CCGG	1 cut(s)	18
BslFI	GGGAC	1 cut(s)	473
BslI	CCNNNNNNNGG	7 cut(s)	82, 161, 208, 238, 621, 830, 1125
BsmFI	GGGAC	1 cut(s)	473
BsnI	GGCC	3 cut(s)	444, 1209, 1314
Bsp1286I	GDGCHC	5 cut(s)	223, 535, 991, 1019, 1459
Bsp1407I	TGTACA	1 cut(s)	1398
Bsp143I	GATC	8 cut(s)	68, 241, 314, 329, 507, 539, 797, 1347
BspACI	CCGC	8 cut(s)	254, 263, 383, 445, 606, 830, 856, 952
BspANI	GGCC	3 cut(s)	444, 1209, 1314
BspCNI	CTCAG	4 cut(s)	94, 139, 496, 769
BspLI	GGNNCC	4 cut(s)	7, 181, 304, 532
BspMI	ACCTGC	1 cut(s)	290
BspOI	GCTAGC	1 cut(s)	389
BspPI	GGATC	5 cut(s)	249, 322, 337, 547, 1355
BspQI	GCTCTTC	1 cut(s)	1227
BspT107I	GGYRCC	2 cut(s)	302, 530
BsrFI	RCCGGY	1 cut(s)	17
BsrGI	TGTACA	1 cut(s)	1398
BsrI	ACTGG	4 cut(s)	328, 588, 1284, 1295
BssAI	RCCGGY	1 cut(s)	17
BssECI	CCNNGG	2 cut(s)	202, 535
BssMI	GATC	8 cut(s)	68, 241, 314, 329, 507, 539, 797, 1347
BssT1I	CCWWGG	1 cut(s)	535
Bst2UI	CCWGG	1 cut(s)	203
Bst4CI	ACNGT	5 cut(s)	148, 310, 1116, 1174, 1357
Bst6I	CTCTTC	1 cut(s)	1227
BstAPI	GCANNNNNTGC	1 cut(s)	986
BstAUI	TGTACA	1 cut(s)	1398
BstC8I	GCNNGC	9 cut(s)	15, 387, 457, 832, 961, 1207, 1218, 1286, 1455
BstDEI	CTNAG	8 cut(s)	23, 81, 126, 357, 504, 777, 1338, 1460
BstENI	CCTNNNNNAGG	1 cut(s)	236
BstF5I	GGATG	2 cut(s)	103, 1028
BstH2I	RGCGCY	1 cut(s)	1206
BstHHI	GCGC	1 cut(s)	1205
BstKTI	GATC	8 cut(s)	71, 244, 317, 332, 510, 542, 800, 1350
BstMBI	GATC	8 cut(s)	68, 241, 314, 329, 507, 539, 797, 1347
BstMWI	GCNNNNNNNGC	8 cut(s)	14, 260, 395, 587, 712, 740, 960, 986
BstNI	CCWGG	1 cut(s)	203
BstNSI	RCATGY	1 cut(s)	1288
BstSCI	CCNGG	1 cut(s)	201
BstSLI	GKGCMC	2 cut(s)	535, 1019
BstV1I	GCAGC	5 cut(s)	590, 896, 992, 1078, 1092
BstV2I	GAAGAC	3 cut(s)	940, 1055, 1376
BstX2I	RGATCY	3 cut(s)	241, 507, 539
BstYI	RGATCY	3 cut(s)	241, 507, 539
Bsu36I	CCTNAGG	1 cut(s)	126
BsuRI	GGCC	3 cut(s)	444, 1209, 1314
BtsCI	GGATG	2 cut(s)	103, 1028
BtsI	GCAGTG	1 cut(s)	961
BtsIMutI	CAGTG	6 cut(s)	581, 961, 1112, 1277, 1288, 1362
BveI	ACCTGC	1 cut(s)	290
Cac8I	GCNNGC	9 cut(s)	15, 387, 457, 832, 961, 1207, 1218, 1286, 1455
CaiI	CAGNNNCTG	1 cut(s)	815
CfoI	GCGC	1 cut(s)	1205
Cfr10I	RCCGGY	1 cut(s)	17
Cfr13I	GGNCC	1 cut(s)	1208
Csp6I	GTAC	3 cut(s)	229, 650, 1399
CviAII	CATG	5 cut(s)	49, 784, 788, 1125, 1285
CviQI	GTAC	3 cut(s)	229, 650, 1399
DdeI	CTNAG	8 cut(s)	23, 81, 126, 357, 504, 777, 1338, 1460
DpnI	GATC	8 cut(s)	70, 243, 316, 331, 509, 541, 799, 1349
DpnII	GATC	8 cut(s)	68, 241, 314, 329, 507, 539, 797, 1347
EaeI	YGGCCR	1 cut(s)	442
Eam1104I	CTCTTC	1 cut(s)	1227
EarI	CTCTTC	1 cut(s)	1227
Eco130I	CCWWGG	1 cut(s)	535
Eco147I	AGGCCT	1 cut(s)	1314
Eco57I	CTGAAG	3 cut(s)	750, 792, 846
Eco81I	CCTNAGG	1 cut(s)	126
EcoNI	CCTNNNNNAGG	1 cut(s)	236
EcoRII	CCWGG	1 cut(s)	201
EcoT14I	CCWWGG	1 cut(s)	535
EcoT22I	ATGCAT	1 cut(s)	789
ErhI	CCWWGG	1 cut(s)	535
FaeI	CATG	5 cut(s)	52, 787, 791, 1128, 1288
FaqI	GGGAC	1 cut(s)	473
FatI	CATG	5 cut(s)	48, 783, 787, 1124, 1284
FauI	CCCGC	1 cut(s)	823
Fnu4HI	GCNGC	8 cut(s)	445, 579, 606, 856, 885, 981, 1092, 1106
FokI	GGATG	2 cut(s)	110, 1015
Fsp4HI	GCNGC	8 cut(s)	445, 579, 606, 856, 885, 981, 1092, 1106
FspBI	CTAG	2 cut(s)	296, 386
GlaI	GCGC	1 cut(s)	1204
GluI	GCNGC	8 cut(s)	445, 579, 606, 856, 885, 981, 1092, 1106
GsaI	CCCAGC	1 cut(s)	915
HaeII	RGCGCY	1 cut(s)	1206
HaeIII	GGCC	3 cut(s)	444, 1209, 1314
HapII	CCGG	1 cut(s)	18
HhaI	GCGC	1 cut(s)	1205
Hin1II	CATG	5 cut(s)	52, 787, 791, 1128, 1288
Hin6I	GCGC	1 cut(s)	1203
HinP1I	GCGC	1 cut(s)	1203
HindIII	AAGCTT	2 cut(s)	11, 1075
HinfI	GANTC	3 cut(s)	106, 571, 1251
HpaII	CCGG	1 cut(s)	18
HphI	GGTGA	2 cut(s)	191, 678
Hpy166II	GTNNAC	3 cut(s)	54, 76, 547
Hpy188I	TCNGA	7 cut(s)	37, 91, 505, 730, 761, 973, 1256
Hpy188III	TCNNGA	5 cut(s)	141, 224, 1248, 1324, 1345
Hpy8I	GTNNAC	3 cut(s)	54, 76, 547
HpyAV	CCTTC	2 cut(s)	232, 1047
HpyCH4III	ACNGT	5 cut(s)	148, 310, 1116, 1174, 1357
HpyF10VI	GCNNNNNNNGC	8 cut(s)	14, 260, 395, 587, 712, 740, 960, 986
HpyF3I	CTNAG	8 cut(s)	23, 81, 126, 357, 504, 777, 1338, 1460
Hsp92II	CATG	5 cut(s)	52, 787, 791, 1128, 1288
HspAI	GCGC	1 cut(s)	1203
Kzo9I	GATC	8 cut(s)	68, 241, 314, 329, 507, 539, 797, 1347
LguI	GCTCTTC	1 cut(s)	1227
LmnI	GCTCC	4 cut(s)	5, 179, 395, 1144
Lsp1109I	GCAGC	5 cut(s)	590, 896, 992, 1078, 1092
LweI	GCATC	1 cut(s)	1037
MaeI	CTAG	2 cut(s)	296, 386
MaeIII	GTNAC	6 cut(s)	472, 772, 897, 1275, 1333, 1468
MalI	GATC	8 cut(s)	70, 243, 316, 331, 509, 541, 799, 1349
MboI	GATC	8 cut(s)	68, 241, 314, 329, 507, 539, 797, 1347
MboII	GAAGA	5 cut(s)	940, 1055, 1214, 1217, 1376
MflI	RGATCY	3 cut(s)	241, 507, 539
MhlI	GDGCHC	5 cut(s)	223, 535, 991, 1019, 1459
MluCI	AATT	4 cut(s)	378, 465, 522, 723
MmeI	TCCRAC	5 cut(s)	292, 373, 539, 976, 1419
MnlI	CCTC	8 cut(s)	127, 135, 148, 618, 772, 1042, 1325, 1435
Mph1103I	ATGCAT	1 cut(s)	789
MroXI	GAANNNNTTC	2 cut(s)	31, 724
MslI	CAYNNNNRTG	1 cut(s)	403
MspA1I	CMGCKG	1 cut(s)	1022
MspI	CCGG	1 cut(s)	18
MspR9I	CCNGG	1 cut(s)	203
MvaI	CCWGG	1 cut(s)	203
MwoI	GCNNNNNNNGC	8 cut(s)	14, 260, 395, 587, 712, 740, 960, 986
NdeII	GATC	8 cut(s)	68, 241, 314, 329, 507, 539, 797, 1347
NheI	GCTAGC	1 cut(s)	385
NlaIII	CATG	5 cut(s)	52, 787, 791, 1128, 1288
NlaIV	GGNNCC	4 cut(s)	7, 181, 304, 532
NmuCI	GTSAC	2 cut(s)	1275, 1468
NsiI	ATGCAT	1 cut(s)	789
NspI	RCATGY	1 cut(s)	1288
PaeI	GCATGC	1 cut(s)	1288
PaqCI	CACCTGC	1 cut(s)	290
PceI	AGGCCT	1 cut(s)	1314
PciSI	GCTCTTC	1 cut(s)	1227
PdmI	GAANNNNTTC	2 cut(s)	31, 724
PfeI	GAWTC	3 cut(s)	106, 571, 1251
PkrI	GCNGC	8 cut(s)	446, 580, 607, 857, 886, 982, 1093, 1107
Psp6I	CCWGG	1 cut(s)	201
PspFI	CCCAGC	1 cut(s)	911
PspGI	CCWGG	1 cut(s)	201
PspN4I	GGNNCC	4 cut(s)	7, 181, 304, 532
PspPI	GGNCC	1 cut(s)	1208
PsrI	GAACNNNNNNTAC	2 cut(s)	642, 674
PstNI	CAGNNNCTG	1 cut(s)	815
PsuI	RGATCY	3 cut(s)	241, 507, 539
PvuII	CAGCTG	1 cut(s)	1022
RsaI	GTAC	3 cut(s)	230, 651, 1400
RsaNI	GTAC	3 cut(s)	229, 650, 1399
RseI	CAYNNNNRTG	1 cut(s)	403
SapI	GCTCTTC	1 cut(s)	1227
SatI	GCNGC	8 cut(s)	445, 579, 606, 856, 885, 981, 1092, 1106
Sau3AI	GATC	8 cut(s)	68, 241, 314, 329, 507, 539, 797, 1347
Sau96I	GGNCC	1 cut(s)	1208
ScrFI	CCNGG	1 cut(s)	203
SduI	GDGCHC	5 cut(s)	223, 535, 991, 1019, 1459
SfaNI	GCATC	1 cut(s)	1037
SmiMI	CAYNNNNRTG	1 cut(s)	403
SmlI	CTYRAG	3 cut(s)	224, 793, 1324
SmoI	CTYRAG	3 cut(s)	224, 793, 1324
SphI	GCATGC	1 cut(s)	1288
Sse9I	AATT	4 cut(s)	378, 465, 522, 723
SseBI	AGGCCT	1 cut(s)	1314
SsiI	CCGC	8 cut(s)	254, 263, 383, 445, 606, 830, 856, 952
SspMI	CTAG	2 cut(s)	296, 386
StuI	AGGCCT	1 cut(s)	1314
StyD4I	CCNGG	1 cut(s)	201
StyI	CCWWGG	1 cut(s)	535
TaaI	ACNGT	5 cut(s)	148, 310, 1116, 1174, 1357
TaqI	TCGA	2 cut(s)	236, 1097
TasI	AATT	4 cut(s)	378, 465, 522, 723
TatI	WGTACW	1 cut(s)	1398
TauI	GCSGC	3 cut(s)	447, 608, 858
TfiI	GAWTC	3 cut(s)	106, 571, 1251
TscAI	CASTG	6 cut(s)	588, 961, 1119, 1284, 1295, 1362
TseFI	GTSAC	2 cut(s)	1275, 1468
TseI	GCWGC	5 cut(s)	578, 884, 980, 1091, 1105
Tsp45I	GTSAC	2 cut(s)	1275, 1468
TspRI	CASTG	6 cut(s)	588, 961, 1119, 1284, 1295, 1362
XagI	CCTNNNNNAGG	1 cut(s)	236
XapI	RAATTY	2 cut(s)	522, 723
XceI	RCATGY	1 cut(s)	1288
XmnI	GAANNNNTTC	2 cut(s)	31, 724
XspI	CTAG	2 cut(s)	296, 386
Zsp2I	ATGCAT	1 cut(s)	789

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.