Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0007158

Physical Location & Seq

Genomic Coordinates Forward (+)

19789616 .. 19803144

Gene Structure 13529 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0217100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3852 bp

ATGGTGGATCCGGCGAATACGCCACTTGGCAAAATGCTATTGGAGGAGATCACTCCGGTCGTCATGGTGCTCCGCACGCCTCTCGTCGAAGAAGCTTGCCAGAAGAACGGCCTCACCTTCGTCCAAATGCTCAGGCCCTTCTGCGTCTTCAACAACTTCGACGTGCCTGTGCGGACGGCGAGTGATCAACCTTACAGGCTTCAGAAGTTCAGATTACGCTTGTTTTATGACTCTGACGTCCGGCAACCGAATTTGGAGGTATCAAAAGAACGGCTGAAACAAGTCATAACCCAGGCTGGGGAGAAAGATCTTTCGGAGTTGTGCTCGGAACTGCCTCAAATTAATAATGCACTGTCCAGACCAGAATCTGAAGCTCTACCATCATGGTTTCAGTTTTTCAACAAAGAGCTTGTACATACAGTCTCTTTTTCTGACCACGAAGCTTTTGATCATCCTTTGGCATGTCTTATTGTGGTTTCTTCAAAAGATGATCAGCCCATCAACAGATTTCTCAACCTCTTTAACTCGAACAAGTTGCCGTCCCTGCTTAACAGTGGGGCAATGGACCCAAATATTTTGAAGCATTATTTACTGGTGCATGATAATCAAGATGGTTCTTCAGAGAAGGCAACTAAACTATTAACTGAAATGAGGAGCACCTTTGGCTCTGATTGTCAATTACTATGCATCAATTCTTCTCAAGATGGTGCAGTTGAACATCAAGATAATCCTTGGGTGCTTTATAGTTCTGAAGACTTACCTAGTCAACCTCTTCGTTGCTTCCTTAACGTTGATGATTTTAATGAGATAAAAGATCTCATGCAAGATTTTTCATCTAAGCACATCATCCCTTACATGGAGCAAAAAATACGTGTGCTGAATCAGCAGGTGTCTGCAACAAGAAAGGGTTTCCGGAACCAAATAAAAAATTTGTGGTGGAGAAAAGGGAAAGACGAGGTGGTGGATTCACCCAGTGGCCCTACGTATACCTTTAGCTCCATTGAATCTCAGATTAGAGTTCTGGGTGATTACGCCTTCATGCTACGTGATTATGAACTTGCATTATCGAACTATCGCCTAATATCAACAGATTATAAGCTTGATAAAGCCTGGAAACGCTTTGCTGGTGCACAGGAAATGATGGGGCTTGCATATTTCATGTTGGACCACTCAAGAAAGGAGGCAGAGAGTTGCATGGATACTGCATTTACTTACTATCTAAAATATGCATTATCTAGTCAGCAGAATGCAACACGATGTGGTCTTTGGTGGGTAGAGATGTTGAAGGCAAGATATCAGTATAGAGAGGCTGCAACTGTTTACTTTCGTGTTGGTGCTGAGGAGCCTCTACATTCTGCTGTAATGCTTGAGCAAGCATCCTATTGCTACTTATTGTCCAAACCACCTATGTTACACAAATATGGGTTTCATCTTGTTCTTTCTGGTGATCGTTACAAAAAATGTGATCAGATCAAACATGCAATTCGCACATATAGAAGTGCCATGTCTGTATATAAAGGAACTACATGGAGCCATATAACAGACCATATCCATTTCCATCTTGGACAGTGGTATGCTCTTCTGGGGTTATATGATTTGGCTGTCTCACATATGTTGGAGGTTCTGGCGTGTAGGCATCAGTCCAAGACAATGCAGGAGTTATTCCTGAAGGACTTCCTTGAAATTGTTCAGAAAACAGGAAAAACATTTGAGGTTTCAAGGCTTCAGTTGCCTGAGATCAATATTCCATCTCTTCGGGTCTTCTTTGAAGATCATCGAACCTATGCATCATCTGCAGCTGCTTCTGTTAAAGAAAGATTATGGCGTTCTTTAGAGGAGGAAATGGTTCCATCTACGTCAACTGGCAGAACTAATTGGCTGGAGTTACAATCAAAGCTTATCCCAAAGAAGTTTAAAGAATCAAATGTCTGTGTTGCAGGAGAAGCAGTCAAGATTGATATTGAGTTTAAGAACCCTCTGCAAATTCCTCTCCTCCTATCCAGTGTTTTCTTAATATGTGAGCTTTTTGCCAACTCTGATGAAATGAAATCCGATGAAAATAATTCATTGGTGGGGGTCCAAAATAATGATGAATCTACCAAATTAAACCACAGGGATGTGGACTCTGAGACATTGTTTTCTTTGTCTGATGTGGACGTTTCAATGGGAGGAGGTGAAACAACTCTGGTGCAACTAACAGTTACTCCCAGAGTAGAAGGTGTTTTACAAATTGTTGGCGTAAAGTGGAAATTATCAGGTTTTGTTGTTGGTTTCCATAACTTTGACACTAGTCCTGTGAAGATAATCGGAAAGCGGAGACAGAAGGCCAATCATCCTCAAATTATTAATTTAAAGTTTGCAGTGGTTAAGAGTGTACCCAAGCTTGAAGGTGTCATTCATTCACTACCTGAAAGGGCGTATGCAGGGGATGTACGGCACCTTGTGTTGGAGTTGAAGAACCAATCAGAATTTGCTGTTAAGAATCTGAAAATGAAGATTAGTCATCCTAGATTCCTAAATGTTGGGCAACGAGAAAGTTTGAACTCAGAGTTTCCTGCTTGCTTAGAAAAGAAAACATCTCAGCATTCTGATATACGTTCCGACTCTGACGCGTCTCATTCTGTGTTTCTATTTCCAGAGGATACTACAATTCAAGGGGAAAAACCATTGCTGTGGCCTCTTTGGTTTCGGGCAGCACTACCTGGGAACATCTCGTTGGACATATCTATATATTATGAGATGGGAGATGTGTCAAGTGGAATTAAATATCGCACTTTACGCATGCATTACAATCTACAGGTATTGCCATCGTTGGATGTGTCATTCCAAATCAGTCCTTGTCCATCAAGATTGCGTGAGTTCCTTGTGCGCATGGATGTTGTCAACAAGACCAGCTCAGAGAGTTTCCAAGTTCATCAGTTATCATCTGTTGGTCAGAAATGGGAGGTCTCATTACTTCAACCTGTCGATGCCATCTTTCCTTCACAATCTTTAATGGCTCATCAAGCATTATCATGCTTCTTCATGCTTAAGAGTCGTAGCAAGCCATCAACTTCTGAAGATGAGACATCCTCTTGTTCCCCGCTTCTGGGAAGTGATGTGACATTGGGTACTGAGGGCAGCTGTGGACCACTCTTCGATATAGCTAGCTCGCCACTGGCGGATTTCCACCATTGTGAAAGACTGCAGCAGGAAATTTCACATAAGGGAGACGTGAATACAGTTGACTTCATATTGATCTCTCGGCCTCTGAAGAATGATACTCACCCTGTGGTTTCTGATCCACCACATCTTTTTTCTCATCATGCATGTCACTGCAGTATCGAGAGCAGAAGTCCCATATCTTGGCTGGTGGATGGACCTCGAACCTTGTATCATAACTTCTCAGCTTCTTTTTGTGAAATAAATTTTCACATGACTATATACAACTCATCAGATGTCATTGCATCTGTATGCATTAAAACCTACGACTCCAACAGTGATAATTTGAGTGATGCAATTCCAGTTCAACCTGCCACGTCTTCTAGCAACCAAGATGGATGGCATGATCTCCCACTAGTTAATGAAATTAGAGTGACTTCTGATGTCGTTGGAGCTCGGACTGGGAAGTCATTGTCAGTGGAAAGTGTTACCCCATTTATTTGGTCTGGGTCAAGCTCTACTAAAGTCGAACTGGAGCCTAAGTCAAGAACTGAGATTCCCCTTCAAGTTTGTGTATTCTCTCCCGGTACATTTGATTTGTCAAATTATGTTTTGCATTGGAAACTTTTGCTTTCTAATGGTGATGGTCTACAGTCTTCTGGGGCATGCCAGGGATATCCCTATTATCTTACCGTGCTCCAGTCTGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0022804 GO:0022857 GO:0022890 GO:0031967 GO:0031975 GO:0034219 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098655 GO:0098660 GO:0098662 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

1283

Amino Acids

145.23

Weight (kDa)

6.14

Isoelectric Point (pI)

49.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TRAPPC-Trs85	PF12739	16 - 431	8.7e-91	ER-Golgi trafficking TRAPP I complex 85 kDa subunit
Ig_TPPC8_1st	PF24545	605 - 756	5.7e-15	TPPC8 first Ig-like domain
Ig_TPPC8_2nd	PF24544	809 - 926	3.7e-08	TPPC8 second Ig-like domain
Ig_TPPC8_C	PF24542	1204 - 1249	4.5e-07	TPPC8 C-terminal Ig-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007158)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16150 AT5G16150 AT5G16150
fragaria_vesca	FvH4_1g25970 FvH4_1g25970 FvH4_1g25970
malus_domestica	MD09G1254500.v1.1 MD17G1246900.v1.1
prunus_persica	Prupe.3G144600_v2.0.a1
pyrus_communis	pycom09g17020 pycom17g24850
rosa_chinensis	RchiOBHm_Chr3g0485241
rosa_laevigata	RLG00000023163
rosa_multiflora	Rmu_sc0005072.1_g000021 Rmu_sc0024791.1_g000001 Rmu_sc0038513.1_g000001
rosa_roxburghii	Rroxscaffold_6G00397150
rosa_rugosa	Rorug03G0217000 Rorug03G0217100
rosa_samantha	Rh3AG266500 Rh3BG302200 Rh3CG300200 Rh3DG296400
rosa_wichuraiana	Rw0G000470 Rw3G023760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1095
AarI	CACCTGC	1 cut(s)	877
AasI	GACNNNNNNGTC	2 cut(s)	236, 3607
AatII	GACGTC	1 cut(s)	240
Acc16I	TGCGCA	1 cut(s)	2870
Acc36I	ACCTGC	2 cut(s)	877, 3520
AccB1I	GGYRCC	1 cut(s)	2436
AccB7I	CCANNNNNTGG	1 cut(s)	3345
AccI	GTMKAC	2 cut(s)	986, 3790
AccII	CGCG	1 cut(s)	2612
AccIII	TCCGGA	1 cut(s)	912
AciI	CCGC	5 cut(s)	73, 172, 2314, 3083, 3161
AclI	AACGTT	1 cut(s)	789
AclWI	GGATC	3 cut(s)	2, 15, 3275
AcsI	RAATTY	6 cut(s)	250, 928, 1983, 2468, 3195, 3406
AcuI	CTGAAG	8 cut(s)	185, 390, 603, 771, 1688, 1709, 3078, 3272
AcyI	GRCGYC	1 cut(s)	237
AdeI	CACNNNGTG	4 cut(s)	974, 1259, 2443, 3271
AfaI	GTAC	5 cut(s)	414, 2376, 2433, 3112, 3730
AfiI	CCNNNNNNNGG	7 cut(s)	297, 298, 856, 2446, 3088, 3089, 3345
AflII	CTTAAG	1 cut(s)	3029
AflIII	ACRYGT	2 cut(s)	871, 2610
AhlI	ACTAGT	2 cut(s)	2288, 3556
AjiI	CACGTC	3 cut(s)	163, 3214, 3519
AjnI	CCWGG	4 cut(s)	291, 1109, 2701, 3810
AjuI	GAANNNNNNNTTGG	2 cut(s)	2699, 2731
AleI	CACNNNNGTG	1 cut(s)	3174
AloI	GAACNNNNNNTCC	2 cut(s)	3682, 3714
Alw21I	GWGCWC	6 cut(s)	72, 326, 659, 1132, 3598, 3840
Alw26I	GTCTC	8 cut(s)	427, 1609, 2123, 2311, 2619, 2953, 3059, 3204
Alw44I	GTGCAC	1 cut(s)	1128
AlwI	GGATC	3 cut(s)	2, 15, 3275
AlwNI	CAGNNNCTG	1 cut(s)	368
Aor13HI	TCCGGA	1 cut(s)	912
AoxI	GGCC	6 cut(s)	109, 134, 976, 2325, 2675, 3245
ApaLI	GTGCAC	1 cut(s)	1128
ApeKI	GCWGC	6 cut(s)	1310, 1796, 1799, 2693, 3120, 3187
ApoI	RAATTY	6 cut(s)	250, 928, 1983, 2468, 3195, 3406
AseI	ATTAAT	2 cut(s)	342, 2346
Asp700I	GAANNNNTTC	3 cut(s)	1673, 1686, 1845
AspLEI	GCGC	1 cut(s)	2871
AspS9I	GGNCC	7 cut(s)	135, 565, 977, 1165, 2077, 3128, 3359
AsuC2I	CCSGG	1 cut(s)	3726
AsuHPI	GGTGA	7 cut(s)	106, 960, 1037, 1457, 2186, 3257, 3794
AsuNHI	GCTAGC	1 cut(s)	3146
AvaII	GGWCC	5 cut(s)	565, 1165, 2077, 3128, 3359
BaeGI	GKGCMC	1 cut(s)	1132
BaeI	ACNNNNGTAYC	2 cut(s)	3304, 3337
BamHI	GGATCC	1 cut(s)	7
BanI	GGYRCC	1 cut(s)	2436
BanII	GRGCYC	1 cut(s)	3598
BarI	GAAGNNNNNNTAC	4 cut(s)	2208, 2240, 3365, 3397
BbsI	GAAGAC	5 cut(s)	139, 759, 1753, 3513, 3789
Bbv12I	GWGCWC	6 cut(s)	72, 326, 659, 1132, 3598, 3840
BbvCI	CCTCAGC	1 cut(s)	1338
BbvI	GCAGC	6 cut(s)	1297, 1786, 1808, 2705, 3132, 3199
BceAI	ACGGC	5 cut(s)	124, 192, 287, 523, 2450
BcgI	CGANNNNNNTGC	4 cut(s)	64, 98, 517, 551
BciT130I	CCWGG	4 cut(s)	293, 1111, 2703, 3812
BciVI	GTATCC	2 cut(s)	1192, 2635
BclI	TGATCA	4 cut(s)	184, 448, 490, 1465
BcnI	CCSGG	1 cut(s)	3726
BcoDI	GTCTC	8 cut(s)	427, 1609, 2123, 2311, 2619, 2953, 3059, 3204
BcuI	ACTAGT	2 cut(s)	2288, 3556
BfaI	CTAG	8 cut(s)	762, 1236, 2289, 2508, 3147, 3525, 3557, 3850
BfmI	CTRYAG	5 cut(s)	1794, 2795, 3185, 3316, 3791
BfrI	CTTAAG	1 cut(s)	3029
BfuAI	ACCTGC	2 cut(s)	877, 3520
BfuI	GTATCC	2 cut(s)	1192, 2635
BglII	AGATCT	2 cut(s)	307, 814
BisI	GCNGC	6 cut(s)	1311, 1797, 1800, 2694, 3121, 3188
BlsI	GCNGC	6 cut(s)	1312, 1798, 1801, 2695, 3122, 3189
Bme1390I	CCNGG	5 cut(s)	293, 1111, 2703, 3726, 3812
Bme18I	GGWCC	5 cut(s)	565, 1165, 2077, 3128, 3359
BmgBI	CACGTC	3 cut(s)	163, 3214, 3519
BmgT120I	GGNCC	7 cut(s)	135, 565, 977, 1165, 2077, 3128, 3359
BmiI	GGNNCC	9 cut(s)	9, 567, 917, 1344, 1532, 1848, 2078, 2438, 3678
BmrFI	CCNGG	5 cut(s)	293, 1111, 2703, 3726, 3812
BmrI	ACTGGG	2 cut(s)	966, 3612
BmsI	GCATC	7 cut(s)	696, 1385, 1645, 1796, 2959, 3455, 3484
BmtI	GCTAGC	1 cut(s)	3150
BmuI	ACTGGG	2 cut(s)	966, 3612
BoxI	GACNNNNGTC	1 cut(s)	2289
BpiI	GAAGAC	5 cut(s)	139, 759, 1753, 3513, 3789
BplI	GAGNNNNNCTC	4 cut(s)	308, 340, 3056, 3088
BpmI	CTGGAG	3 cut(s)	1901, 3695, 3824
Bpu10I	CCTNAGC	2 cut(s)	131, 1338
BpuEI	CTTGAG	3 cut(s)	684, 1156, 1388
BpuMI	CCSGG	1 cut(s)	3726
BsaAI	YACGTR	3 cut(s)	872, 984, 1046
BsaHI	GRCGYC	1 cut(s)	237
BsaI	GGTCTC	1 cut(s)	2953
BsaJI	CCNNGG	4 cut(s)	291, 731, 2702, 3811
BsaWI	WCCGGW	2 cut(s)	55, 912
BsaXI	ACNNNNNCTCC	6 cut(s)	1932, 1962, 2188, 2218, 3668, 3698
Bsc4I	CCNNNNNNNGG	7 cut(s)	297, 298, 856, 2446, 3088, 3089, 3345
Bse1I	ACTGG	9 cut(s)	597, 972, 1867, 2001, 3162, 3503, 3607, 3678, 3841
Bse3DI	GCAATG	3 cut(s)	567, 2666, 3441
BseAI	TCCGGA	1 cut(s)	912
BseBI	CCWGG	4 cut(s)	293, 1111, 2703, 3812
BseDI	CCNNGG	4 cut(s)	291, 731, 2702, 3811
BseLI	CCNNNNNNNGG	7 cut(s)	297, 298, 856, 2446, 3088, 3089, 3345
BseMI	GCAATG	3 cut(s)	567, 2666, 3441
BseNI	ACTGG	9 cut(s)	597, 972, 1867, 2001, 3162, 3503, 3607, 3678, 3841
BseRI	GAGGAG	6 cut(s)	59, 667, 1355, 1850, 1982, 2184
BseSI	GKGCMC	1 cut(s)	1132
BseXI	GCAGC	6 cut(s)	1297, 1786, 1808, 2705, 3132, 3199
BseYI	CCCAGC	1 cut(s)	296
BsgI	GTGCAG	1 cut(s)	729
Bsh1236I	CGCG	1 cut(s)	2612
Bsh1285I	CGRYCG	1 cut(s)	60
BshFI	GGCC	6 cut(s)	111, 136, 978, 2327, 2677, 3247
BshNI	GGYRCC	1 cut(s)	2436
BsiEI	CGRYCG	1 cut(s)	60
BsiHKAI	GWGCWC	6 cut(s)	72, 326, 659, 1132, 3598, 3840
BsiSI	CCGG	5 cut(s)	11, 56, 241, 913, 3726
BslFI	GGGAC	2 cut(s)	526, 3321
BslI	CCNNNNNNNGG	7 cut(s)	297, 298, 856, 2446, 3088, 3089, 3345
BsmAI	GTCTC	8 cut(s)	427, 1609, 2123, 2311, 2619, 2953, 3059, 3204
BsmBI	CGTCTC	2 cut(s)	2619, 3204
BsmFI	GGGAC	2 cut(s)	526, 3321
BsmI	GAATGC	2 cut(s)	1252, 2584
BsnI	GGCC	6 cut(s)	111, 136, 978, 2327, 2677, 3247
Bso31I	GGTCTC	1 cut(s)	2953
Bsp1286I	GDGCHC	6 cut(s)	72, 326, 659, 1132, 3598, 3840
Bsp13I	TCCGGA	1 cut(s)	912
Bsp1407I	TGTACA	1 cut(s)	412
BspACI	CCGC	5 cut(s)	73, 172, 2314, 3083, 3161
BspANI	GGCC	6 cut(s)	111, 136, 978, 2327, 2677, 3247
BspEI	TCCGGA	1 cut(s)	912
BspFNI	CGCG	1 cut(s)	2612
BspLI	GGNNCC	9 cut(s)	9, 567, 917, 1344, 1532, 1848, 2078, 2438, 3678
BspMAI	CTGCAG	3 cut(s)	1798, 3189, 3320
BspMI	ACCTGC	2 cut(s)	877, 3520
BspOI	GCTAGC	1 cut(s)	3150
BspPI	GGATC	3 cut(s)	2, 15, 3275
BspQI	GCTCTTC	1 cut(s)	1584
BspT107I	GGYRCC	1 cut(s)	2436
BspTI	CTTAAG	1 cut(s)	3029
BspTNI	GGTCTC	1 cut(s)	2953
BsrDI	GCAATG	3 cut(s)	567, 2666, 3441
BsrGI	TGTACA	1 cut(s)	412
BsrI	ACTGG	9 cut(s)	597, 972, 1867, 2001, 3162, 3503, 3607, 3678, 3841
BssECI	CCNNGG	4 cut(s)	291, 731, 2702, 3811
BssNAI	GTATAC	1 cut(s)	987
BssNI	GRCGYC	1 cut(s)	237
BssT1I	CCWWGG	1 cut(s)	731
Bst1107I	GTATAC	1 cut(s)	987
Bst2UI	CCWGG	4 cut(s)	293, 1111, 2703, 3812
Bst6I	CTCTTC	4 cut(s)	777, 1584, 1758, 3140
BstACI	GRCGYC	1 cut(s)	237
BstAFI	CTTAAG	1 cut(s)	3029
BstAPI	GCANNNNNTGC	1 cut(s)	1793
BstAUI	TGTACA	1 cut(s)	412
BstBAI	YACGTR	3 cut(s)	872, 984, 1046
BstFNI	CGCG	1 cut(s)	2612
BstHHI	GCGC	1 cut(s)	2871
BstMAI	GTCTC	8 cut(s)	427, 1609, 2123, 2311, 2619, 2953, 3059, 3204
BstMCI	CGRYCG	1 cut(s)	60
BstMWI	GCNNNNNNNGC	9 cut(s)	76, 544, 663, 883, 1729, 1793, 1943, 2778, 3005
BstNI	CCWGG	4 cut(s)	293, 1111, 2703, 3812
BstNSI	RCATGY	5 cut(s)	465, 1481, 2785, 3312, 3810
BstPAI	GACNNNNGTC	1 cut(s)	2289
BstSCI	CCNGG	5 cut(s)	291, 1109, 2701, 3724, 3810
BstSFI	CTRYAG	5 cut(s)	1794, 2795, 3185, 3316, 3791
BstSLI	GKGCMC	1 cut(s)	1132
BstSNI	TACGTA	1 cut(s)	984
BstUI	CGCG	1 cut(s)	2612
BstV1I	GCAGC	6 cut(s)	1297, 1786, 1808, 2705, 3132, 3199
BstV2I	GAAGAC	5 cut(s)	139, 759, 1753, 3513, 3789
BstX2I	RGATCY	3 cut(s)	7, 307, 814
BstXI	CCANNNNNNTGG	2 cut(s)	2673, 3642
BstYI	RGATCY	3 cut(s)	7, 307, 814
BstZ17I	GTATAC	1 cut(s)	987
BsuI	GTATCC	2 cut(s)	1192, 2635
BsuRI	GGCC	6 cut(s)	111, 136, 978, 2327, 2677, 3247
BtrI	CACGTC	3 cut(s)	163, 3214, 3519
BtsI	GCAGTG	2 cut(s)	2367, 3313
BveI	ACCTGC	2 cut(s)	877, 3520
CaiI	CAGNNNCTG	1 cut(s)	368
CfoI	GCGC	1 cut(s)	2871
Cfr13I	GGNCC	7 cut(s)	135, 565, 977, 1165, 2077, 3128, 3359
CseI	GACGC	3 cut(s)	133, 2601, 2618
Csp6I	GTAC	5 cut(s)	413, 2375, 2432, 3111, 3729
CviQI	GTAC	5 cut(s)	413, 2375, 2432, 3111, 3729
DraI	TTTAAA	2 cut(s)	1915, 2352
DraIII	CACNNNGTG	4 cut(s)	974, 1259, 2443, 3271
DrdI	GACNNNNNNGTC	2 cut(s)	236, 3607
DseDI	GACNNNNNNGTC	2 cut(s)	236, 3607
Eam1104I	CTCTTC	4 cut(s)	777, 1584, 1758, 3140
EarI	CTCTTC	4 cut(s)	777, 1584, 1758, 3140
EciI	GGCGGA	1 cut(s)	3176
Ecl136II	GAGCTC	1 cut(s)	3596
Eco105I	TACGTA	1 cut(s)	984
Eco130I	CCWWGG	1 cut(s)	731
Eco24I	GRGCYC	1 cut(s)	3598
Eco31I	GGTCTC	1 cut(s)	2953
Eco32I	GATATC	2 cut(s)	1295, 3818
Eco47I	GGWCC	5 cut(s)	565, 1165, 2077, 3128, 3359
Eco53kI	GAGCTC	1 cut(s)	3596
Eco57I	CTGAAG	8 cut(s)	185, 390, 603, 771, 1688, 1709, 3078, 3272
EcoICRI	GAGCTC	1 cut(s)	3596
EcoO109I	RGGNCCY	1 cut(s)	135
EcoRII	CCWGG	4 cut(s)	291, 1109, 2701, 3810
EcoRV	GATATC	2 cut(s)	1295, 3818
EcoT14I	CCWWGG	1 cut(s)	731
EcoT22I	ATGCAT	6 cut(s)	689, 1231, 1789, 2787, 3310, 3458
EcoT38I	GRGCYC	1 cut(s)	3598
ErhI	CCWWGG	1 cut(s)	731
Esp3I	CGTCTC	2 cut(s)	2619, 3204
FaqI	GGGAC	2 cut(s)	526, 3321
FauI	CCCGC	1 cut(s)	3090
FauNDI	CATATG	1 cut(s)	1611
FbaI	TGATCA	4 cut(s)	184, 448, 490, 1465
FblI	GTMKAC	2 cut(s)	986, 3790
Fnu4HI	GCNGC	6 cut(s)	1311, 1797, 1800, 2694, 3121, 3188
FriOI	GRGCYC	1 cut(s)	3598
Fsp4HI	GCNGC	6 cut(s)	1311, 1797, 1800, 2694, 3121, 3188
FspAI	RTGCGCAY	1 cut(s)	2870
FspBI	CTAG	8 cut(s)	762, 1236, 2289, 2508, 3147, 3525, 3557, 3850
FspI	TGCGCA	1 cut(s)	2870
GlaI	GCGC	1 cut(s)	2870
GluI	GCNGC	6 cut(s)	1311, 1797, 1800, 2694, 3121, 3188
GsaI	CCCAGC	1 cut(s)	300
GsuI	CTGGAG	3 cut(s)	1901, 3695, 3824
HaeIII	GGCC	6 cut(s)	111, 136, 978, 2327, 2677, 3247
HapII	CCGG	5 cut(s)	11, 56, 241, 913, 3726
HgaI	GACGC	3 cut(s)	133, 2601, 2618
HhaI	GCGC	1 cut(s)	2871
Hin1I	GRCGYC	1 cut(s)	237
Hin6I	GCGC	1 cut(s)	2869
HinP1I	GCGC	1 cut(s)	2869
HincII	GTYRAC	4 cut(s)	767, 1860, 2884, 3226
HindII	GTYRAC	4 cut(s)	767, 1860, 2884, 3226
HindIII	AAGCTT	5 cut(s)	93, 441, 1097, 1895, 2381
HpaII	CCGG	5 cut(s)	11, 56, 241, 913, 3726
HphI	GGTGA	7 cut(s)	106, 960, 1037, 1457, 2186, 3257, 3794
Hpy99I	CGWCG	2 cut(s)	89, 164
HpyF10VI	GCNNNNNNNGC	9 cut(s)	76, 544, 663, 883, 1729, 1793, 1943, 2778, 3005
Hsp92I	GRCGYC	1 cut(s)	237
HspAI	GCGC	1 cut(s)	2869
Kpn2I	TCCGGA	1 cut(s)	912
Ksp22I	TGATCA	4 cut(s)	184, 448, 490, 1465
LguI	GCTCTTC	1 cut(s)	1584
LmnI	GCTCC	9 cut(s)	75, 654, 859, 1001, 1342, 1530, 3593, 3676, 3843
Lsp1109I	GCAGC	6 cut(s)	1297, 1786, 1808, 2705, 3132, 3199
LweI	GCATC	7 cut(s)	696, 1385, 1645, 1796, 2959, 3455, 3484
MaeI	CTAG	8 cut(s)	762, 1236, 2289, 2508, 3147, 3525, 3557, 3850
MaeIII	GTNAC	8 cut(s)	1410, 1451, 1884, 2200, 3100, 3311, 3574, 3628
MflI	RGATCY	3 cut(s)	7, 307, 814
MhlI	GDGCHC	6 cut(s)	72, 326, 659, 1132, 3598, 3840
MluI	ACGCGT	1 cut(s)	2610
MlyI	GAGTC	5 cut(s)	224, 2117, 2597, 3043, 3464
MmeI	TCCRAC	8 cut(s)	1143, 1596, 2427, 2625, 2697, 2793, 3498, 3571
Mph1103I	ATGCAT	6 cut(s)	689, 1231, 1789, 2787, 3310, 3458
MroI	TCCGGA	1 cut(s)	912
MroXI	GAANNNNTTC	3 cut(s)	1673, 1686, 1845
MslI	CAYNNNNRTG	6 cut(s)	1419, 2115, 2671, 3013, 3174, 3451
MspA1I	CMGCKG	2 cut(s)	1799, 3123
MspCI	CTTAAG	1 cut(s)	3029
MspI	CCGG	5 cut(s)	11, 56, 241, 913, 3726
MspR9I	CCNGG	5 cut(s)	293, 1111, 2703, 3726, 3812
Mva1269I	GAATGC	2 cut(s)	1252, 2584
MvaI	CCWGG	4 cut(s)	293, 1111, 2703, 3812
MvnI	CGCG	1 cut(s)	2612
MwoI	GCNNNNNNNGC	9 cut(s)	76, 544, 663, 883, 1729, 1793, 1943, 2778, 3005
NciI	CCSGG	1 cut(s)	3726
NdeI	CATATG	1 cut(s)	1611
NheI	GCTAGC	1 cut(s)	3146
NlaIV	GGNNCC	9 cut(s)	9, 567, 917, 1344, 1532, 1848, 2078, 2438, 3678
NmeAIII	GCCGAG	1 cut(s)	3223
NmuCI	GTSAC	3 cut(s)	3100, 3311, 3574
NsbI	TGCGCA	1 cut(s)	2870
NsiI	ATGCAT	6 cut(s)	689, 1231, 1789, 2787, 3310, 3458
NspI	RCATGY	5 cut(s)	465, 1481, 2785, 3312, 3810
OliI	CACNNNNGTG	1 cut(s)	3174
PaeI	GCATGC	2 cut(s)	2785, 3810
PaqCI	CACCTGC	1 cut(s)	877
PciSI	GCTCTTC	1 cut(s)	1584
PctI	GAATGC	2 cut(s)	1252, 2584
PdmI	GAANNNNTTC	3 cut(s)	1673, 1686, 1845
PfeI	GAWTC	9 cut(s)	365, 880, 965, 1004, 1919, 2093, 2482, 2511, 3697
PflMI	CCANNNNNTGG	1 cut(s)	3345
PkrI	GCNGC	6 cut(s)	1312, 1798, 1801, 2695, 3122, 3189
PleI	GAGTC	5 cut(s)	224, 2117, 2597, 3042, 3464
PpsI	GAGTC	5 cut(s)	224, 2117, 2597, 3042, 3464
Ppu21I	YACGTR	3 cut(s)	872, 984, 1046
PshAI	GACNNNNGTC	1 cut(s)	2289
PshBI	ATTAAT	2 cut(s)	342, 2346
PsiI	TTATAA	1 cut(s)	1095
Psp124BI	GAGCTC	1 cut(s)	3598
Psp1406I	AACGTT	1 cut(s)	789
Psp6I	CCWGG	4 cut(s)	291, 1109, 2701, 3810
PspFI	CCCAGC	1 cut(s)	296
PspGI	CCWGG	4 cut(s)	291, 1109, 2701, 3810
PspN4I	GGNNCC	9 cut(s)	9, 567, 917, 1344, 1532, 1848, 2078, 2438, 3678
PspPI	GGNCC	7 cut(s)	135, 565, 977, 1165, 2077, 3128, 3359
PstI	CTGCAG	3 cut(s)	1798, 3189, 3320
PstNI	CAGNNNCTG	1 cut(s)	368
PsuI	RGATCY	3 cut(s)	7, 307, 814
PvuII	CAGCTG	2 cut(s)	1799, 3123
RsaI	GTAC	5 cut(s)	414, 2376, 2433, 3112, 3730
RsaNI	GTAC	5 cut(s)	413, 2375, 2432, 3111, 3729
RseI	CAYNNNNRTG	6 cut(s)	1419, 2115, 2671, 3013, 3174, 3451
SacI	GAGCTC	1 cut(s)	3598
SapI	GCTCTTC	1 cut(s)	1584
SatI	GCNGC	6 cut(s)	1311, 1797, 1800, 2694, 3121, 3188
Sau96I	GGNCC	7 cut(s)	135, 565, 977, 1165, 2077, 3128, 3359
SchI	GAGTC	5 cut(s)	224, 2117, 2597, 3043, 3464
ScrFI	CCNGG	5 cut(s)	293, 1111, 2703, 3726, 3812
SduI	GDGCHC	6 cut(s)	72, 326, 659, 1132, 3598, 3840
SfaNI	GCATC	7 cut(s)	696, 1385, 1645, 1796, 2959, 3455, 3484
SfcI	CTRYAG	5 cut(s)	1794, 2795, 3185, 3316, 3791
SinI	GGWCC	5 cut(s)	565, 1165, 2077, 3128, 3359
SmiMI	CAYNNNNRTG	6 cut(s)	1419, 2115, 2671, 3013, 3174, 3451
SmlI	CTYRAG	4 cut(s)	699, 1171, 1367, 3029
SmoI	CTYRAG	4 cut(s)	699, 1171, 1367, 3029
SnaBI	TACGTA	1 cut(s)	984
SpeI	ACTAGT	2 cut(s)	2288, 3556
SphI	GCATGC	2 cut(s)	2785, 3810
SsiI	CCGC	5 cut(s)	73, 172, 2314, 3083, 3161
SspI	AATATT	2 cut(s)	574, 1744
SspMI	CTAG	8 cut(s)	762, 1236, 2289, 2508, 3147, 3525, 3557, 3850
SstI	GAGCTC	1 cut(s)	3598
StyD4I	CCNGG	5 cut(s)	291, 1109, 2701, 3724, 3810
StyI	CCWWGG	1 cut(s)	731
TatI	WGTACW	1 cut(s)	412
TfiI	GAWTC	9 cut(s)	365, 880, 965, 1004, 1919, 2093, 2482, 2511, 3697
TseFI	GTSAC	3 cut(s)	3100, 3311, 3574
TseI	GCWGC	6 cut(s)	1310, 1796, 1799, 2693, 3120, 3187
Tsp45I	GTSAC	3 cut(s)	3100, 3311, 3574
Van91I	CCANNNNNTGG	1 cut(s)	3345
Vha464I	CTTAAG	1 cut(s)	3029
VneI	GTGCAC	1 cut(s)	1128
VpaK11BI	GGWCC	5 cut(s)	565, 1165, 2077, 3128, 3359
VspI	ATTAAT	2 cut(s)	342, 2346
XapI	RAATTY	6 cut(s)	250, 928, 1983, 2468, 3195, 3406
XceI	RCATGY	5 cut(s)	465, 1481, 2785, 3312, 3810
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1559, 3346
XmiI	GTMKAC	2 cut(s)	986, 3790
XmnI	GAANNNNTTC	3 cut(s)	1673, 1686, 1845
XspI	CTAG	8 cut(s)	762, 1236, 2289, 2508, 3147, 3525, 3557, 3850
ZraI	GACGTC	1 cut(s)	238
Zsp2I	ATGCAT	6 cut(s)	689, 1231, 1789, 2787, 3310, 3458

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.