Prupe.4G172300_v2.0.a1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0003297
Physical Location & Seq
Forward (+)
10208698 .. 10210752
2055 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G172300.1

Sequence Viewer

Length: 651 bp
ATGGCCTACAGAGCAGATGACGACTACGACTACCTGTTCAAGGTGGTGCTCATAGGCGACTCTGGTGTTGGAAAATCCAACCTTTTGTCCAGGTTCACGCGCAACGAGTTCAGCCTCGAGTCTAAGTCTACCATCGGCGTCGAGTTCGCCACTCGGAGCATACACGTTGATGAGAAGATCGTCAAGGCCCAGATTTGGGACACCGCCGGCCAAGAAAGGTACCGTGCAATTACAAGTGCATACTACCGAGGAGCTGTTGGTGCTTTGCTTGTCTATGACGTCACCCGGCATGTTACGTTTGAGAATGTTGAAAGATGGTTAAAGGAGCTACGGGATCATACAGACTCCAATATCGTGATAATGCTCGTGGGTAACAAGGCTGACCTGCGTCACCTGCGTGCTGTTTCTGTTGAGGATGCTAAGGCCTTCGCTGAGAGAGAAAACACCTTCTTTATGGAGACATCAGCACTTGAGTCTATGAACGTTGAAAATGCATTCACTGAGGTCCTAACACAAATATACCATGTAGTTAGCCGGAAAGCACTTGAGGTTGGGGATGATCCTGCAGCACTACCTAAGGGACAAACCATAAATGTTGGAAACAAGGATGATGTTTCAGCTGTTAAGAAAGCTGGTTGCTGTTCTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

217

Amino Acids

24.14

Weight (kDa)

5.6

Isoelectric Point (pI)

25.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 402
AatII GACGTC 1 cut(s) 282
Acc36I ACCTGC 2 cut(s) 393, 402
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 1 cut(s) 219
AccI GTMKAC 1 cut(s) 128
AccII CGCG 1 cut(s) 100
AciI CCGC 1 cut(s) 204
AclI AACGTT 1 cut(s) 483
AclWI GGATC 2 cut(s) 342, 554
AcoI YGGCCR 1 cut(s) 208
AcyI GRCGYC 2 cut(s) 138, 279
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 3 cut(s) 40, 195, 196
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 3 cut(s) 40, 311, 488
AjnI CCWGG 1 cut(s) 89
AleI CACNNNNGTG 1 cut(s) 396
AluBI AGCT 4 cut(s) 254, 328, 620, 632
AluI AGCT 4 cut(s) 254, 328, 620, 632
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 2 cut(s) 342, 554
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 4 cut(s) 3, 186, 208, 423
ApeKI GCWGC 1 cut(s) 566
Asp718I GGTACC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 2 cut(s) 187, 505
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 2 cut(s) 274, 383
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 505
AxyI CCTNAGG 1 cut(s) 576
BanI GGYRCC 1 cut(s) 219
BauI CACGAG 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 578
BccI CCATC 2 cut(s) 140, 309
BciT130I CCWGG 1 cut(s) 91
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 452
BfmI CTRYAG 2 cut(s) 7, 564
BfuAI ACCTGC 2 cut(s) 393, 402
BisI GCNGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 568
Bme1390I CCNGG 2 cut(s) 91, 286
Bme18I GGWCC 1 cut(s) 505
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 2 cut(s) 187, 505
BmiI GGNNCC 1 cut(s) 221
BmrFI CCNGG 2 cut(s) 91, 286
BmsI GCATC 1 cut(s) 406
BoxI GACNNNNGTC 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 420
BpuEI CTTGAG 2 cut(s) 491, 566
BpuMI CCSGG 1 cut(s) 286
BsaHI GRCGYC 2 cut(s) 138, 279
BsaJI CCNNGG 1 cut(s) 247
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 195, 196
Bse118I RCCGGY 1 cut(s) 206
Bse21I CCTNAGG 1 cut(s) 576
BseBI CCWGG 1 cut(s) 91
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 3 cut(s) 421, 562, 613
BseLI CCNNNNNNNGG 3 cut(s) 40, 195, 196
BseMII CTCAG 2 cut(s) 423, 492
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 1 cut(s) 578
Bsh1236I CGCG 1 cut(s) 100
BshFI GGCC 4 cut(s) 5, 188, 210, 425
BshNI GGYRCC 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 3 cut(s) 207, 286, 535
BslFI GGGAC 2 cut(s) 212, 594
BslI CCNNNNNNNGG 3 cut(s) 40, 195, 196
BsmAI GTCTC 1 cut(s) 452
BsmFI GGGAC 2 cut(s) 212, 594
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 4 cut(s) 5, 188, 210, 425
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 3 cut(s) 177, 334, 559
BspACI CCGC 1 cut(s) 204
BspANI GGCC 4 cut(s) 5, 188, 210, 425
BspCNI CTCAG 2 cut(s) 424, 493
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 1 cut(s) 221
BspMAI CTGCAG 1 cut(s) 568
BspMI ACCTGC 2 cut(s) 393, 402
BspPI GGATC 2 cut(s) 342, 554
BspT107I GGYRCC 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 3 cut(s) 177, 334, 559
BssNI GRCGYC 2 cut(s) 138, 279
BssSI CACGAG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 1 cut(s) 224
BstACI GRCGYC 2 cut(s) 138, 279
BstC8I GCNNGC 2 cut(s) 208, 399
BstDEI CTNAG 5 cut(s) 123, 420, 432, 501, 576
BstENI CCTNNNNNAGG 1 cut(s) 38
BstF5I GGATG 3 cut(s) 421, 562, 613
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 3 cut(s) 180, 337, 562
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 3 cut(s) 177, 334, 559
BstMWI GCNNNNNNNGC 3 cut(s) 11, 260, 394
BstNI CCWGG 1 cut(s) 91
BstNSI RCATGY 1 cut(s) 293
BstPAI GACNNNNGTC 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 89, 284
BstSFI CTRYAG 2 cut(s) 7, 564
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 1 cut(s) 578
Bsu36I CCTNAGG 1 cut(s) 576
BsuRI GGCC 4 cut(s) 5, 188, 210, 425
BtsCI GGATG 3 cut(s) 421, 562, 613
BtsIMutI CAGTG 1 cut(s) 498
BveI ACCTGC 2 cut(s) 393, 402
Cac8I GCNNGC 2 cut(s) 208, 399
CfoI GCGC 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 2 cut(s) 187, 505
CseI GACGC 2 cut(s) 127, 377
Csp6I GTAC 1 cut(s) 220
CviAII CATG 2 cut(s) 290, 524
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 5 cut(s) 123, 420, 432, 501, 576
DpnI GATC 3 cut(s) 179, 336, 561
DpnII GATC 3 cut(s) 177, 334, 559
EaeI YGGCCR 1 cut(s) 208
Eco147I AGGCCT 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 505
Eco81I CCTNAGG 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 116
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 505
EcoRII CCWGG 1 cut(s) 89
EcoT22I ATGCAT 1 cut(s) 496
FaeI CATG 2 cut(s) 293, 527
FaqI GGGAC 2 cut(s) 212, 594
FatI CATG 2 cut(s) 289, 523
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 567
FokI GGATG 3 cut(s) 428, 569, 620
Fsp4HI GCNGC 1 cut(s) 567
GlaI GCGC 1 cut(s) 101
GluI GCNGC 1 cut(s) 567
HaeIII GGCC 4 cut(s) 5, 188, 210, 425
HapII CCGG 3 cut(s) 207, 286, 535
HgaI GACGC 2 cut(s) 127, 377
HhaI GCGC 1 cut(s) 102
Hin1I GRCGYC 2 cut(s) 138, 279
Hin1II CATG 2 cut(s) 293, 527
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HinfI GANTC 4 cut(s) 59, 119, 344, 473
HpaII CCGG 3 cut(s) 207, 286, 535
HphI GGTGA 2 cut(s) 274, 383
Hpy166II GTNNAC 2 cut(s) 96, 129
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 1 cut(s) 355
Hpy8I GTNNAC 2 cut(s) 96, 129
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 436, 457
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 4 cut(s) 165, 279, 296, 483
HpyCH4V TGCA 4 cut(s) 227, 239, 494, 566
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 260, 394
HpyF3I CTNAG 5 cut(s) 123, 420, 432, 501, 576
HpySE526I ACGT 4 cut(s) 165, 279, 296, 483
Hsp92I GRCGYC 2 cut(s) 138, 279
Hsp92II CATG 2 cut(s) 293, 527
HspAI GCGC 1 cut(s) 100
KpnI GGTACC 1 cut(s) 223
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 3 cut(s) 177, 334, 559
LmnI GCTCC 3 cut(s) 156, 251, 325
Lsp1109I GCAGC 1 cut(s) 578
LweI GCATC 1 cut(s) 406
MaeII ACGT 4 cut(s) 165, 279, 296, 483
MaeIII GTNAC 4 cut(s) 280, 292, 371, 389
MalI GATC 3 cut(s) 179, 336, 561
MboI GATC 3 cut(s) 177, 334, 559
MboII GAAGA 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 1 cut(s) 228
MlyI GAGTC 4 cut(s) 53, 128, 338, 482
MmeI TCCRAC 3 cut(s) 49, 102, 577
MnlI CCTC 5 cut(s) 125, 242, 406, 496, 541
Mph1103I ATGCAT 1 cut(s) 496
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 2 cut(s) 320, 624
MslI CAYNNNNRTG 2 cut(s) 168, 396
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 3 cut(s) 207, 286, 535
MspR9I CCNGG 2 cut(s) 91, 286
Mva1269I GAATGC 1 cut(s) 494
MvaI CCWGG 1 cut(s) 91
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 3 cut(s) 11, 260, 394
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 286
NdeII GATC 3 cut(s) 177, 334, 559
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 2 cut(s) 293, 527
NlaIV GGNNCC 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 280, 389
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 1 cut(s) 293
OliI CACNNNNGTG 1 cut(s) 396
PaeR7I CTCGAG 1 cut(s) 116
PaqCI CACCTGC 1 cut(s) 402
PceI AGGCCT 1 cut(s) 425
PctI GAATGC 1 cut(s) 494
PdiI GCCGGC 1 cut(s) 208
PkrI GCNGC 1 cut(s) 568
PleI GAGTC 4 cut(s) 53, 127, 338, 481
PpsI GAGTC 4 cut(s) 53, 127, 338, 481
PpuMI RGGWCCY 1 cut(s) 505
PshAI GACNNNNGTC 1 cut(s) 387
Psp1406I AACGTT 1 cut(s) 483
Psp5II RGGWCCY 1 cut(s) 505
Psp6I CCWGG 1 cut(s) 89
PspGI CCWGG 1 cut(s) 89
PspN4I GGNNCC 1 cut(s) 221
PspPI GGNCC 2 cut(s) 187, 505
PspPPI RGGWCCY 1 cut(s) 505
PspXI VCTCGAGB 1 cut(s) 116
PstI CTGCAG 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 2 cut(s) 168, 396
SaqAI TTAA 2 cut(s) 320, 624
SatI GCNGC 1 cut(s) 567
Sau3AI GATC 3 cut(s) 177, 334, 559
Sau96I GGNCC 2 cut(s) 187, 505
SchI GAGTC 4 cut(s) 53, 128, 338, 482
ScrFI CCNGG 2 cut(s) 91, 286
SduI GDGCHC 1 cut(s) 51
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 2 cut(s) 7, 564
Sfr274I CTCGAG 1 cut(s) 116
SinI GGWCC 1 cut(s) 505
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 2 cut(s) 168, 396
SmlI CTYRAG 3 cut(s) 116, 470, 545
SmoI CTYRAG 3 cut(s) 116, 470, 545
Sse9I AATT 1 cut(s) 228
SseBI AGGCCT 1 cut(s) 425
SsiI CCGC 1 cut(s) 204
StuI AGGCCT 1 cut(s) 425
StyD4I CCNGG 2 cut(s) 89, 284
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 4 cut(s) 168, 282, 299, 486
TaqI TCGA 2 cut(s) 117, 141
TasI AATT 1 cut(s) 228
Tru1I TTAA 2 cut(s) 320, 624
Tru9I TTAA 2 cut(s) 320, 624
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 2 cut(s) 280, 389
TseI GCWGC 1 cut(s) 566
Tsp45I GTSAC 2 cut(s) 280, 389
TspDTI ATGAA 1 cut(s) 494
TspRI CASTG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 505
XagI CCTNNNNNAGG 1 cut(s) 38
XceI RCATGY 1 cut(s) 293
XhoI CTCGAG 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 128
ZraI GACGTC 1 cut(s) 280
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.