Rh5BG222700

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003297
Physical Location & Seq
Forward (+)
25816175 .. 25818136
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG222700.1

Sequence Viewer

Length: 651 bp
ATGGCCTACAGGTCGGACGACGACTACGACTACCTCTTCAAGGTCGTCTTGATCGGCGATTCCGGCGTCGGCAAATCCAACCTACTGTCCAGGTTCACGCGCAACGAGTTCAGCCTCGAGTCTAAGTCCACCATCGGCGTCGAGTTCGCCACCCGCAGCATTCGCGTCGATGATAAGATCGTCAAGGCCCAGATTTGGGACACCGCCGGCCAAGAAAGGTACCGAGCAATTACAAGTGCATACTACCGAGGAGCTGTAGGTGCTTTACTCGTCTATGATGTCACCCGACATGTTACATTTGAGAATGTTGAGAGATGGTTAAAGGAGCTACGGGATCACACAGACACCAATATAGTGATAATGCTTGTGGGTAACAAGGCCGACCTGCGTCACCTACGTGCTGTTTCTGTTGAGGATGCTAAAGCCTTTGCTGAGAGAGAAAACACCTTCTTTATGGAGACGTCAGCACTCGAGTCTATGAATGTTGAAAATGCATTCACTGAAGTCTTAACCCAAATACATAATGTTGTGAGCCGGAAAGCACTTGAGGTTGGAGATGATCCTGCAGCCGTGCCTAAGGGACAAACCATAAATGTTGGAGGCAAGGATGATGTTTCAGCCATTAAGAAAGATGGGTGTTGTTCTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

216

Amino Acids

24.11

Weight (kDa)

5.47

Isoelectric Point (pI)

24.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 13 - 168 2.8e-12 ADP-ribosylation factor family
Ras PF00071 14 - 174 3.3e-64 Ras family
Roc PF08477 14 - 128 3.3e-35 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 14 - 120 8.7e-07 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 393
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 1 cut(s) 219
AccII CGCG 2 cut(s) 100, 165
AciI CCGC 2 cut(s) 154, 204
AclWI GGATC 2 cut(s) 342, 554
AcoI YGGCCR 1 cut(s) 208
AcuI CTGAAG 1 cut(s) 522
AcyI GRCGYC 3 cut(s) 66, 138, 461
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 3 cut(s) 40, 195, 196
AflIII ACRYGT 1 cut(s) 289
AgsI TTSAA 2 cut(s) 40, 488
AjnI CCWGG 1 cut(s) 89
AleI CACNNNNGTG 1 cut(s) 396
AluBI AGCT 2 cut(s) 254, 328
AluI AGCT 2 cut(s) 254, 328
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 2 cut(s) 342, 554
Ama87I CYCGRG 2 cut(s) 116, 470
AoxI GGCC 4 cut(s) 3, 186, 208, 378
ApeKI GCWGC 2 cut(s) 156, 566
Asp718I GGTACC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 1 cut(s) 187
AsuHPI GGTGA 2 cut(s) 274, 383
AvaI CYCGRG 2 cut(s) 116, 470
AxyI CCTNAGG 1 cut(s) 576
BanI GGYRCC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 168, 578
BccI CCATC 3 cut(s) 140, 309, 626
BceAI ACGGC 1 cut(s) 554
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 452
BfmI CTRYAG 3 cut(s) 7, 255, 564
BfuAI ACCTGC 1 cut(s) 393
BisI GCNGC 2 cut(s) 157, 567
BlsI GCNGC 2 cut(s) 158, 568
Bme1390I CCNGG 1 cut(s) 91
BmeT110I CYCGRG 2 cut(s) 116, 470
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 91
BmsI GCATC 1 cut(s) 406
BoxI GACNNNNGTC 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 566
BsaAI YACGTR 1 cut(s) 398
BsaHI GRCGYC 3 cut(s) 66, 138, 461
BsaJI CCNNGG 1 cut(s) 247
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 195, 196
Bse118I RCCGGY 1 cut(s) 206
Bse21I CCTNAGG 1 cut(s) 576
BseBI CCWGG 1 cut(s) 91
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 2 cut(s) 421, 613
BseLI CCNNNNNNNGG 3 cut(s) 40, 195, 196
BseMII CTCAG 1 cut(s) 423
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 2 cut(s) 168, 578
Bsh1236I CGCG 2 cut(s) 100, 165
BshFI GGCC 4 cut(s) 5, 188, 210, 380
BshNI GGYRCC 1 cut(s) 219
BsiHKCI CYCGRG 2 cut(s) 116, 470
BsiSI CCGG 3 cut(s) 63, 207, 535
BslFI GGGAC 2 cut(s) 212, 594
BslI CCNNNNNNNGG 3 cut(s) 40, 195, 196
BsmAI GTCTC 1 cut(s) 452
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 2 cut(s) 212, 594
BsmI GAATGC 2 cut(s) 159, 494
BsnI GGCC 4 cut(s) 5, 188, 210, 380
BsoBI CYCGRG 2 cut(s) 116, 470
Bsp143I GATC 4 cut(s) 51, 177, 334, 559
BspACI CCGC 2 cut(s) 154, 204
BspANI GGCC 4 cut(s) 5, 188, 210, 380
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 2 cut(s) 100, 165
BspLI GGNNCC 1 cut(s) 221
BspMAI CTGCAG 1 cut(s) 568
BspMI ACCTGC 1 cut(s) 393
BspPI GGATC 2 cut(s) 342, 554
BspT107I GGYRCC 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 4 cut(s) 51, 177, 334, 559
BssNI GRCGYC 3 cut(s) 66, 138, 461
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 3 cut(s) 66, 138, 461
BstBAI YACGTR 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 3 cut(s) 123, 432, 576
BstENI CCTNNNNNAGG 1 cut(s) 38
BstF5I GGATG 2 cut(s) 421, 613
BstFNI CGCG 2 cut(s) 100, 165
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 4 cut(s) 54, 180, 337, 562
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 4 cut(s) 51, 177, 334, 559
BstMWI GCNNNNNNNGC 3 cut(s) 63, 162, 260
BstNI CCWGG 1 cut(s) 91
BstNSI RCATGY 1 cut(s) 293
BstPAI GACNNNNGTC 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 89
BstSFI CTRYAG 3 cut(s) 7, 255, 564
BstUI CGCG 2 cut(s) 100, 165
BstV1I GCAGC 2 cut(s) 168, 578
Bsu36I CCTNAGG 1 cut(s) 576
BsuRI GGCC 4 cut(s) 5, 188, 210, 380
BtsCI GGATG 2 cut(s) 421, 613
BtsIMutI CAGTG 1 cut(s) 498
BveI ACCTGC 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 208
CfoI GCGC 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 187
CseI GACGC 4 cut(s) 55, 127, 154, 377
Csp6I GTAC 1 cut(s) 220
CviAII CATG 1 cut(s) 290
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 3 cut(s) 123, 432, 576
DpnI GATC 4 cut(s) 53, 179, 336, 561
DpnII GATC 4 cut(s) 51, 177, 334, 559
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 522
Eco81I CCTNAGG 1 cut(s) 576
Eco88I CYCGRG 2 cut(s) 116, 470
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 89
EcoT22I ATGCAT 1 cut(s) 496
Esp3I CGTCTC 1 cut(s) 452
FaeI CATG 1 cut(s) 293
FaiI YATR 8 cut(s) 241, 276, 291, 353, 455, 479, 522, 590
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FaqI GGGAC 2 cut(s) 212, 594
FatI CATG 1 cut(s) 289
FauI CCCGC 1 cut(s) 161
Fnu4HI GCNGC 2 cut(s) 157, 567
FokI GGATG 2 cut(s) 428, 620
Fsp4HI GCNGC 2 cut(s) 157, 567
GlaI GCGC 1 cut(s) 101
GluI GCNGC 2 cut(s) 157, 567
HaeIII GGCC 4 cut(s) 5, 188, 210, 380
HapII CCGG 3 cut(s) 63, 207, 535
HgaI GACGC 4 cut(s) 55, 127, 154, 377
HhaI GCGC 1 cut(s) 102
Hin1I GRCGYC 3 cut(s) 66, 138, 461
Hin1II CATG 1 cut(s) 293
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HinfI GANTC 3 cut(s) 59, 119, 473
HpaII CCGG 3 cut(s) 63, 207, 535
HphI GGTGA 2 cut(s) 274, 383
Hpy166II GTNNAC 2 cut(s) 96, 129
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 49
Hpy8I GTNNAC 2 cut(s) 96, 129
Hpy99I CGWCG 4 cut(s) 23, 71, 143, 170
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4IV ACGT 2 cut(s) 397, 461
HpyCH4V TGCA 3 cut(s) 239, 494, 566
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 162, 260
HpyF3I CTNAG 3 cut(s) 123, 432, 576
HpySE526I ACGT 2 cut(s) 397, 461
Hsp92I GRCGYC 3 cut(s) 66, 138, 461
Hsp92II CATG 1 cut(s) 293
HspAI GCGC 1 cut(s) 100
KpnI GGTACC 1 cut(s) 223
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 4 cut(s) 51, 177, 334, 559
LmnI GCTCC 2 cut(s) 251, 325
LpnPI CCDG 8 cut(s) 76, 76, 103, 203, 220, 398, 548, 576
Lsp1109I GCAGC 2 cut(s) 168, 578
LweI GCATC 1 cut(s) 406
MaeII ACGT 2 cut(s) 397, 461
MaeIII GTNAC 4 cut(s) 280, 292, 371, 389
MalI GATC 4 cut(s) 53, 179, 336, 561
MboI GATC 4 cut(s) 51, 177, 334, 559
MboII GAAGA 1 cut(s) 28
MluCI AATT 1 cut(s) 228
MlyI GAGTC 2 cut(s) 128, 482
MmeI TCCRAC 3 cut(s) 102, 532, 577
MnlI CCTC 6 cut(s) 44, 125, 242, 406, 541, 593
Mph1103I ATGCAT 1 cut(s) 496
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 3 cut(s) 320, 509, 624
MslI CAYNNNNRTG 1 cut(s) 396
MspI CCGG 3 cut(s) 63, 207, 535
MspR9I CCNGG 1 cut(s) 91
Mva1269I GAATGC 2 cut(s) 159, 494
MvaI CCWGG 1 cut(s) 91
MvnI CGCG 2 cut(s) 100, 165
MwoI GCNNNNNNNGC 3 cut(s) 63, 162, 260
NaeI GCCGGC 1 cut(s) 208
NdeII GATC 4 cut(s) 51, 177, 334, 559
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 1 cut(s) 293
NlaIV GGNNCC 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 280, 389
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 1 cut(s) 293
OliI CACNNNNGTG 1 cut(s) 396
PaeR7I CTCGAG 2 cut(s) 116, 470
PciI ACATGT 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 2 cut(s) 159, 494
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 158, 568
PleI GAGTC 2 cut(s) 127, 481
PpsI GAGTC 2 cut(s) 127, 481
Ppu21I YACGTR 1 cut(s) 398
PscI ACATGT 1 cut(s) 289
PshAI GACNNNNGTC 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 89
PspGI CCWGG 1 cut(s) 89
PspN4I GGNNCC 1 cut(s) 221
PspPI GGNCC 1 cut(s) 187
PspXI VCTCGAGB 2 cut(s) 116, 470
PstI CTGCAG 1 cut(s) 568
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 3 cut(s) 320, 509, 624
SatI GCNGC 2 cut(s) 157, 567
Sau3AI GATC 4 cut(s) 51, 177, 334, 559
Sau96I GGNCC 1 cut(s) 187
SchI GAGTC 2 cut(s) 128, 482
ScrFI CCNGG 1 cut(s) 91
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 3 cut(s) 7, 255, 564
Sfr274I CTCGAG 2 cut(s) 116, 470
SlaI CTCGAG 2 cut(s) 116, 470
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 3 cut(s) 116, 470, 545
SmoI CTYRAG 3 cut(s) 116, 470, 545
Sse9I AATT 1 cut(s) 228
SsiI CCGC 2 cut(s) 154, 204
StyD4I CCNGG 1 cut(s) 89
TaaI ACNGT 1 cut(s) 87
TaiI ACGT 2 cut(s) 400, 464
TaqI TCGA 4 cut(s) 117, 141, 168, 471
TasI AATT 1 cut(s) 228
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 3 cut(s) 320, 509, 624
Tru9I TTAA 3 cut(s) 320, 509, 624
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 2 cut(s) 280, 389
TseI GCWGC 2 cut(s) 156, 566
Tsp45I GTSAC 2 cut(s) 280, 389
TspDTI ATGAA 1 cut(s) 494
TspRI CASTG 1 cut(s) 505
XagI CCTNNNNNAGG 1 cut(s) 38
XceI RCATGY 1 cut(s) 293
XhoI CTCGAG 2 cut(s) 116, 470
ZraI GACGTC 1 cut(s) 462
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.