pycom05g28420

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003297
Physical Location & Seq
Forward (+)
29159805 .. 29160936
1132 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g28420.1

Sequence Viewer

Length: 654 bp
ATGGGGGCATACAGAGCCGACGACGATTACGATTACTTGTTCAAGGTGGTGTTGATCGGCGACTCGGGCGTCGGAAAATCGAACCTTTTGGCGCGGTTCACGAAGAACGAGTTCAGCCTCGAATCCAAGTCCACCATTGGCGTCGAGTTCGCCACCAGAAGCATTCGGGTCGATGATAAGGTCGTCAAGGCCCAGATTTGGGACACCGCCGGCCAGGAAAGGTATCGTGCGATCACAAGCGCATACTATCGAGGTGCCGTTGGGGCCTTGCTTGTCTATGACGTTACTCGTCATGTTACGTTTGAAAATGTGGAAAGATGGTTGAAGGAGCTTCGCGATCACACGGACGCCAACATTGTGATCATGCTGGTGGGAAACAAGGGAGATCTGCGTCACCTGCGAGCCGTTTCCACCGATGATGCTAAGGCGTTTGCTGAACGAGAGAACACCTTTTTCATGGAAACTTCTGCCCTCGAGTCCATGAATGTCGAAAATGCTTTCACCGAAGTGCTGACGCAAATCCATCATGTTGTGAGCAGGAAGGACCTTGACATCGGGGATGACCCAGCAGCCTTGCCCAAGGGACAGACGATCAATGTTGGGGGCAAGGACGATGTATCAGCTGTGAAGCAAGACGGGTGTTGTTCTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ras

Protein Analysis

218

Amino Acids

24.13

Weight (kDa)

5.28

Isoelectric Point (pI)

22.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 166 3.8e-12 ADP-ribosylation factor family
Ras PF00071 15 - 175 6.2e-64 Ras family
Roc PF08477 15 - 129 5.7e-35 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 15 - 121 9.9e-07 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 405
AasI GACNNNNNNGTC 1 cut(s) 68
Acc36I ACCTGC 1 cut(s) 405
AccB1I GGYRCC 1 cut(s) 254
AccII CGCG 2 cut(s) 94, 336
AciI CCGC 2 cut(s) 94, 207
AcoI YGGCCR 1 cut(s) 211
AcyI GRCGYC 3 cut(s) 69, 141, 348
AfiI CCNNNNNNNGG 2 cut(s) 198, 199
AgsI TTSAA 3 cut(s) 43, 305, 325
AjnI CCWGG 1 cut(s) 213
AleI CACNNNNGTG 1 cut(s) 506
AluBI AGCT 2 cut(s) 331, 623
AluI AGCT 2 cut(s) 331, 623
Ama87I CYCGRG 2 cut(s) 64, 473
AoxI GGCC 3 cut(s) 189, 211, 264
ApeKI GCWGC 1 cut(s) 569
Asp700I GAANNNNTTC 1 cut(s) 110
AspLEI GCGC 2 cut(s) 94, 242
AspS9I GGNCC 3 cut(s) 190, 264, 544
AsuHPI GGTGA 2 cut(s) 386, 493
AvaI CYCGRG 2 cut(s) 64, 473
AvaII GGWCC 1 cut(s) 544
BanI GGYRCC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 581
BccI CCATC 2 cut(s) 312, 531
BceAI ACGGC 2 cut(s) 242, 389
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 1 cut(s) 215
BclI TGATCA 1 cut(s) 360
BfuAI ACCTGC 1 cut(s) 405
BglI GCCNNNNNGGC 1 cut(s) 263
BglII AGATCT 1 cut(s) 385
BisI GCNGC 1 cut(s) 570
BlsI GCNGC 1 cut(s) 571
Bme1390I CCNGG 1 cut(s) 215
Bme18I GGWCC 1 cut(s) 544
BmeT110I CYCGRG 2 cut(s) 64, 473
BmgT120I GGNCC 3 cut(s) 190, 264, 544
BmiI GGNNCC 2 cut(s) 256, 265
BmrFI CCNGG 1 cut(s) 215
BmsI GCATC 1 cut(s) 409
Bpu10I CCTNAGC 1 cut(s) 423
BsaAI YACGTR 1 cut(s) 651
BsaHI GRCGYC 3 cut(s) 69, 141, 348
BsaJI CCNNGG 1 cut(s) 579
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 199
Bse118I RCCGGY 1 cut(s) 209
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 1 cut(s) 565
BseLI CCNNNNNNNGG 2 cut(s) 198, 199
BseXI GCAGC 1 cut(s) 581
BseYI CCCAGC 1 cut(s) 565
Bsh1236I CGCG 2 cut(s) 94, 336
BshFI GGCC 3 cut(s) 191, 213, 266
BshNI GGYRCC 1 cut(s) 254
BsiHKCI CYCGRG 2 cut(s) 64, 473
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 2 cut(s) 215, 597
BslI CCNNNNNNNGG 2 cut(s) 198, 199
BsmFI GGGAC 2 cut(s) 215, 597
BsmI GAATGC 1 cut(s) 162
BsnI GGCC 3 cut(s) 191, 213, 266
BsoBI CYCGRG 2 cut(s) 64, 473
Bsp143I GATC 6 cut(s) 54, 231, 337, 360, 385, 591
Bsp68I TCGCGA 1 cut(s) 336
BspACI CCGC 2 cut(s) 94, 207
BspANI GGCC 3 cut(s) 191, 213, 266
BspFNI CGCG 2 cut(s) 94, 336
BspLI GGNNCC 2 cut(s) 256, 265
BspMI ACCTGC 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 254
BsrFI RCCGGY 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 6 cut(s) 54, 231, 337, 360, 385, 591
BssNI GRCGYC 3 cut(s) 69, 141, 348
BssT1I CCWWGG 1 cut(s) 579
Bst2UI CCWGG 1 cut(s) 215
BstACI GRCGYC 3 cut(s) 69, 141, 348
BstBAI YACGTR 1 cut(s) 651
BstC8I GCNNGC 2 cut(s) 211, 402
BstDEI CTNAG 1 cut(s) 423
BstF5I GGATG 1 cut(s) 565
BstFNI CGCG 2 cut(s) 94, 336
BstHHI GCGC 2 cut(s) 94, 242
BstKTI GATC 6 cut(s) 57, 234, 340, 363, 388, 594
BstMBI GATC 6 cut(s) 54, 231, 337, 360, 385, 591
BstMWI GCNNNNNNNGC 4 cut(s) 14, 66, 263, 397
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 213
BstSNI TACGTA 1 cut(s) 651
BstUI CGCG 2 cut(s) 94, 336
BstV1I GCAGC 1 cut(s) 581
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 3 cut(s) 191, 213, 266
BtsCI GGATG 1 cut(s) 565
BtuMI TCGCGA 1 cut(s) 336
BveI ACCTGC 1 cut(s) 405
Cac8I GCNNGC 2 cut(s) 211, 402
CfoI GCGC 2 cut(s) 94, 242
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 3 cut(s) 190, 264, 544
CseI GACGC 5 cut(s) 58, 130, 356, 380, 523
CviAII CATG 5 cut(s) 293, 364, 457, 481, 527
CviJI RGCY 9 cut(s) 17, 117, 191, 213, 266, 331, 404, 572, 623
CviKI_1 RGCY 9 cut(s) 17, 117, 191, 213, 266, 331, 404, 572, 623
DdeI CTNAG 1 cut(s) 423
DpnI GATC 6 cut(s) 56, 233, 339, 362, 387, 593
DpnII GATC 6 cut(s) 54, 231, 337, 360, 385, 591
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
EaeI YGGCCR 1 cut(s) 211
Eco105I TACGTA 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 579
Eco47I GGWCC 1 cut(s) 544
Eco88I CYCGRG 2 cut(s) 64, 473
EcoO109I RGGNCCY 2 cut(s) 264, 544
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 579
FaeI CATG 5 cut(s) 296, 367, 460, 484, 530
FaiI YATR 8 cut(s) 10, 244, 279, 294, 365, 458, 482, 528
FaqI GGGAC 2 cut(s) 215, 597
FatI CATG 5 cut(s) 292, 363, 456, 480, 526
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 1 cut(s) 570
FokI GGATG 1 cut(s) 572
Fsp4HI GCNGC 1 cut(s) 570
GlaI GCGC 2 cut(s) 93, 241
GluI GCNGC 1 cut(s) 570
GsaI CCCAGC 1 cut(s) 569
HaeIII GGCC 3 cut(s) 191, 213, 266
HapII CCGG 1 cut(s) 210
HgaI GACGC 5 cut(s) 58, 130, 356, 380, 523
HhaI GCGC 2 cut(s) 94, 242
Hin1I GRCGYC 3 cut(s) 69, 141, 348
Hin1II CATG 5 cut(s) 296, 367, 460, 484, 530
Hin6I GCGC 2 cut(s) 92, 240
HinP1I GCGC 2 cut(s) 92, 240
HinfI GANTC 3 cut(s) 62, 122, 476
HpaII CCGG 1 cut(s) 210
HphI GGTGA 2 cut(s) 386, 493
Hpy166II GTNNAC 2 cut(s) 99, 132
Hpy188I TCNGA 1 cut(s) 74
Hpy188III TCNNGA 2 cut(s) 100, 335
Hpy8I GTNNAC 2 cut(s) 99, 132
Hpy99I CGWCG 4 cut(s) 23, 26, 74, 146
HpyAV CCTTC 2 cut(s) 319, 535
HpyCH4IV ACGT 3 cut(s) 282, 299, 650
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 66, 263, 397
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 3 cut(s) 282, 299, 650
Hsp92I GRCGYC 3 cut(s) 69, 141, 348
Hsp92II CATG 5 cut(s) 296, 367, 460, 484, 530
HspAI GCGC 2 cut(s) 92, 240
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 6 cut(s) 54, 231, 337, 360, 385, 591
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 9 cut(s) 169, 200, 206, 223, 227, 353, 410, 523, 579
Lsp1109I GCAGC 1 cut(s) 581
LweI GCATC 1 cut(s) 409
MaeII ACGT 3 cut(s) 282, 299, 650
MaeIII GTNAC 3 cut(s) 283, 295, 392
MalI GATC 6 cut(s) 56, 233, 339, 362, 387, 593
MboI GATC 6 cut(s) 54, 231, 337, 360, 385, 591
MboII GAAGA 1 cut(s) 115
MflI RGATCY 1 cut(s) 385
MlyI GAGTC 2 cut(s) 56, 485
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 3 cut(s) 128, 245, 482
MroNI GCCGGC 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 110
MslI CAYNNNNRTG 2 cut(s) 368, 506
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 215
Mva1269I GAATGC 1 cut(s) 162
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 2 cut(s) 94, 336
MwoI GCNNNNNNNGC 4 cut(s) 14, 66, 263, 397
NaeI GCCGGC 1 cut(s) 211
NdeII GATC 6 cut(s) 54, 231, 337, 360, 385, 591
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 5 cut(s) 296, 367, 460, 484, 530
NlaIV GGNNCC 2 cut(s) 256, 265
NmuCI GTSAC 1 cut(s) 392
NruI TCGCGA 1 cut(s) 336
OliI CACNNNNGTG 1 cut(s) 506
PaeR7I CTCGAG 1 cut(s) 473
PaqCI CACCTGC 1 cut(s) 405
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 122
PkrI GCNGC 1 cut(s) 571
PleI GAGTC 2 cut(s) 56, 484
PpsI GAGTC 2 cut(s) 56, 484
Ppu21I YACGTR 1 cut(s) 651
PpuMI RGGWCCY 1 cut(s) 544
Psp5II RGGWCCY 1 cut(s) 544
Psp6I CCWGG 1 cut(s) 213
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 256, 265
PspPI GGNCC 3 cut(s) 190, 264, 544
PspPPI RGGWCCY 1 cut(s) 544
PspXI VCTCGAGB 1 cut(s) 473
PsuI RGATCY 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 623
RruI TCGCGA 1 cut(s) 336
RseI CAYNNNNRTG 2 cut(s) 368, 506
SatI GCNGC 1 cut(s) 570
Sau3AI GATC 6 cut(s) 54, 231, 337, 360, 385, 591
Sau96I GGNCC 3 cut(s) 190, 264, 544
SchI GAGTC 2 cut(s) 56, 485
ScrFI CCNGG 1 cut(s) 215
SfaNI GCATC 1 cut(s) 409
Sfr274I CTCGAG 1 cut(s) 473
SinI GGWCC 1 cut(s) 544
SlaI CTCGAG 1 cut(s) 473
SmiMI CAYNNNNRTG 2 cut(s) 368, 506
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
SnaBI TACGTA 1 cut(s) 651
SsiI CCGC 2 cut(s) 94, 207
StyD4I CCNGG 1 cut(s) 213
StyI CCWWGG 1 cut(s) 579
TaiI ACGT 3 cut(s) 285, 302, 653
TaqI TCGA 7 cut(s) 80, 120, 144, 171, 250, 474, 489
TfiI GAWTC 1 cut(s) 122
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 1 cut(s) 569
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 2 cut(s) 445, 497
TspGWI ACGGA 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 544
XhoI CTCGAG 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.