Prupe.7G254700_v2.0.a1

Homeobox protein knotted-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0006503
Physical Location & Seq
Forward (+)
21408695 .. 21411189
2495 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G254700.1

Sequence Viewer

Length: 1110 bp
ATGGCGTTTCATCATCATCATCATCACCAGCAGCAGCAAACCCCTCCAGAAATGGCGTTCCACTCCTTCGCCTCGGACCAGCCGCCGCTCTCCGGAGCTCCGACGTGGCTCAACAACGCCGCGTTTCGACAACAGAACAGCTTCCTCCACGACGCGCGAAACGACGACGTCGTCATATCACCGTCGGGGAAGAGCAGCAACTGCAGCGGCCGGAACCGGAGAGAGATAAGCGGCTACGACGGCGAAGAGGAGGAAGAAGACGAACTGGAGTGCGAGAGCGCGAGGTTCAAAGCGGACTTAGTGGGCCACCCTCTGTACGAGCAGCTTGTATCGGCTCACGTCTCGTGCTTGAGGATCGCCACCCCCGTCGATCAGCTGCCGAGGATCGACGAGCAGCTCGTGCAGTCGCAGCGCGTGGTGGATAAGTACTCTGCGCTTCGAGCCAATGGGGATGTCCAAGTCATGGATGAGAAAGAACTTGATTTATTCATGACAAACTATGTTCTACTGCTATGCTCCTTCAAAGAACAATTGCAGCAACATGTCCGAGTTCATGCCATGGAAGCAGTGATGGCTTGTTGGGAGCTCGATCAATCTCTACAAAGCTTAACAGGTGTGTCAACGGGTGAAGGCACCGGTGCTACAATGTCCGACGACGACGACCAGGTCGACAGTGACATCAACTCGTATGATGGAAGCCTGGACGGGCCTGACACCATGGGATTCGGTCCTCTCGTTCCGACTGAGAGTGAGAGGTCCTTAATGGAGCGTGTAAGGCAAGAATTGAAGCATGAACTGAAACAGGGTTACAAGGAAAAGATTGTAGACATTAGGGAGGAAATTCTACGCAAGAGAAGAGCAGGTAAACTGCCAGGTGACACCACTTCTGTCCTAAAATCTTGGTGGCAATCACATTCTAAGTGGCCTTACCCTACGGAGGAAGACAAAGCCAGGTTGGTGCAGGAAACAGGCTTGCAATTGAAACAGATCAATAACTGGTTCATAAATCAAAGAAAAAGGAATTGGCACAGTAACATTTCCTCAACTTCTGTTTTGAAGAGCAAACGCAAGAGTAATGCAGGTGACATCAGCAGCCAACGATTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ELK Homeobox_KN KNOX1 KNOX2

Protein Analysis

370

Amino Acids

42.0

Weight (kDa)

5.62

Isoelectric Point (pI)

52.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1070
AasI GACNNNNNNGTC 2 cut(s) 170, 665
AatII GACGTC 1 cut(s) 171
Acc36I ACCTGC 2 cut(s) 851, 1070
AccB1I GGYRCC 1 cut(s) 632
AccB7I CCANNNNNTGG 1 cut(s) 463
AccBSI CCGCTC 1 cut(s) 88
AccI GTMKAC 2 cut(s) 669, 825
AccII CGCG 5 cut(s) 122, 155, 157, 281, 414
AccIII TCCGGA 1 cut(s) 92
AciI CCGC 6 cut(s) 83, 86, 120, 207, 231, 293
AclWI GGATC 2 cut(s) 362, 392
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 840
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 2 cut(s) 317, 428
AfiI CCNNNNNNNGG 3 cut(s) 92, 463, 937
AflIII ACRYGT 1 cut(s) 541
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 6 cut(s) 289, 523, 787, 982, 1057, 1105
AjiI CACGTC 2 cut(s) 105, 340
AjnI CCWGG 4 cut(s) 663, 699, 871, 950
AjuI GAANNNNNNNTTGG 2 cut(s) 1006, 1038
AluBI AGCT 7 cut(s) 98, 141, 325, 376, 397, 586, 606
AluI AGCT 7 cut(s) 98, 141, 325, 376, 397, 586, 606
Alw21I GWGCWC 2 cut(s) 100, 588
Alw26I GTCTC 1 cut(s) 346
AlwI GGATC 2 cut(s) 362, 392
AlwNI CAGNNNCTG 1 cut(s) 201
Aor13HI TCCGGA 1 cut(s) 92
AoxI GGCC 4 cut(s) 208, 304, 707, 923
ApoI RAATTY 1 cut(s) 840
AsiGI ACCGGT 1 cut(s) 635
Asp700I GAANNNNTTC 1 cut(s) 140
AspLEI GCGC 4 cut(s) 157, 281, 414, 436
AspS9I GGNCC 5 cut(s) 76, 304, 707, 728, 756
AsuHPI GGTGA 5 cut(s) 17, 171, 638, 887, 1094
AvaII GGWCC 3 cut(s) 76, 728, 756
BanI GGYRCC 1 cut(s) 632
BanII GRGCYC 2 cut(s) 100, 588
BauI CACGAG 2 cut(s) 343, 398
BbsI GAAGAC 2 cut(s) 264, 948
Bbv12I GWGCWC 2 cut(s) 100, 588
BccI CCATC 2 cut(s) 565, 686
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 4 cut(s) 665, 701, 873, 952
BcoDI GTCTC 1 cut(s) 346
BfmI CTRYAG 1 cut(s) 202
BfuAI ACCTGC 2 cut(s) 851, 1070
BmcAI AGTACT 1 cut(s) 428
Bme1390I CCNGG 4 cut(s) 665, 701, 873, 952
Bme18I GGWCC 3 cut(s) 76, 728, 756
BmgBI CACGTC 2 cut(s) 105, 340
BmgT120I GGNCC 5 cut(s) 76, 304, 707, 728, 756
BmiI GGNNCC 2 cut(s) 215, 634
BmrFI CCNGG 4 cut(s) 665, 701, 873, 952
BpiI GAAGAC 2 cut(s) 264, 948
BpmI CTGGAG 2 cut(s) 30, 287
BpuEI CTTGAG 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 4 cut(s) 72, 380, 558, 717
BsaWI WCCGGW 3 cut(s) 92, 216, 635
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 463, 937
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 2 cut(s) 270, 1001
BseAI TCCGGA 1 cut(s) 92
BseBI CCWGG 4 cut(s) 665, 701, 873, 952
BseDI CCNNGG 4 cut(s) 72, 380, 558, 717
BseGI GGATG 2 cut(s) 457, 472
BseLI CCNNNNNNNGG 3 cut(s) 92, 463, 937
BseMII CTCAG 1 cut(s) 735
BseNI ACTGG 2 cut(s) 270, 1001
BseRI GAGGAG 1 cut(s) 263
BseX3I CGGCCG 1 cut(s) 208
BsgI GTGCAG 2 cut(s) 422, 980
Bsh1236I CGCG 5 cut(s) 122, 155, 157, 281, 414
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 4 cut(s) 210, 306, 709, 925
BshNI GGYRCC 1 cut(s) 632
BshTI ACCGGT 1 cut(s) 635
BsiEI CGRYCG 1 cut(s) 211
BsiHKAI GWGCWC 2 cut(s) 100, 588
BsiSI CCGG 4 cut(s) 93, 211, 217, 636
BslI CCNNNNNNNGG 3 cut(s) 92, 463, 937
BsmAI GTCTC 1 cut(s) 346
BsmBI CGTCTC 1 cut(s) 346
BsnI GGCC 4 cut(s) 210, 306, 709, 925
Bsp1286I GDGCHC 2 cut(s) 100, 588
Bsp13I TCCGGA 1 cut(s) 92
Bsp143I GATC 5 cut(s) 354, 370, 384, 589, 987
Bsp19I CCATGG 2 cut(s) 558, 717
BspACI CCGC 6 cut(s) 83, 86, 120, 207, 231, 293
BspANI GGCC 4 cut(s) 210, 306, 709, 925
BspCNI CTCAG 1 cut(s) 736
BspEI TCCGGA 1 cut(s) 92
BspFNI CGCG 5 cut(s) 122, 155, 157, 281, 414
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 2 cut(s) 215, 634
BspMAI CTGCAG 1 cut(s) 206
BspMI ACCTGC 2 cut(s) 851, 1070
BspPI GGATC 2 cut(s) 362, 392
BspQI GCTCTTC 3 cut(s) 185, 850, 1052
BspT107I GGYRCC 1 cut(s) 632
BsrBI CCGCTC 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 2 cut(s) 270, 1001
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 4 cut(s) 72, 380, 558, 717
BssMI GATC 5 cut(s) 354, 370, 384, 589, 987
BssNI GRCGYC 1 cut(s) 168
BssSI CACGAG 2 cut(s) 343, 398
BssT1I CCWWGG 2 cut(s) 558, 717
Bst2BI CACGAG 2 cut(s) 343, 398
Bst2UI CCWGG 4 cut(s) 665, 701, 873, 952
Bst4CI ACNGT 3 cut(s) 183, 674, 1031
Bst6I CTCTTC 4 cut(s) 185, 240, 850, 1052
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 2 cut(s) 201, 400
BstC8I GCNNGC 1 cut(s) 974
BstDEI CTNAG 3 cut(s) 298, 744, 918
BstDSI CCRYGG 2 cut(s) 558, 717
BstF5I GGATG 2 cut(s) 457, 472
BstFNI CGCG 5 cut(s) 122, 155, 157, 281, 414
BstHHI GCGC 4 cut(s) 157, 281, 414, 436
BstKTI GATC 5 cut(s) 357, 373, 387, 592, 990
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 5 cut(s) 354, 370, 384, 589, 987
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 9 cut(s) 201, 204, 240, 400, 409, 440, 563, 572, 775
BstNI CCWGG 4 cut(s) 665, 701, 873, 952
BstNSI RCATGY 1 cut(s) 545
BstSCI CCNGG 4 cut(s) 663, 699, 871, 950
BstSFI CTRYAG 1 cut(s) 202
BstUI CGCG 5 cut(s) 122, 155, 157, 281, 414
BstV2I GAAGAC 2 cut(s) 264, 948
BstZI CGGCCG 1 cut(s) 208
BsuRI GGCC 4 cut(s) 210, 306, 709, 925
BtgI CCRYGG 2 cut(s) 558, 717
BtrI CACGTC 2 cut(s) 105, 340
BtsCI GGATG 2 cut(s) 457, 472
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 2 cut(s) 573, 679
BveI ACCTGC 2 cut(s) 851, 1070
Cac8I GCNNGC 1 cut(s) 974
CaiI CAGNNNCTG 1 cut(s) 201
CciI TCATGA 1 cut(s) 489
CfoI GCGC 4 cut(s) 157, 281, 414, 436
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 5 cut(s) 76, 304, 707, 728, 756
CseI GACGC 1 cut(s) 161
CsiI ACCWGGT 1 cut(s) 663
Csp6I GTAC 2 cut(s) 316, 427
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 7 cut(s) 463, 490, 542, 554, 559, 718, 791
CviQI GTAC 2 cut(s) 316, 427
DdeI CTNAG 3 cut(s) 298, 744, 918
DpnI GATC 5 cut(s) 356, 372, 386, 591, 989
DpnII GATC 5 cut(s) 354, 370, 384, 589, 987
DrdI GACNNNNNNGTC 2 cut(s) 170, 665
DseDI GACNNNNNNGTC 2 cut(s) 170, 665
EaeI YGGCCR 1 cut(s) 208
EagI CGGCCG 1 cut(s) 208
Eam1104I CTCTTC 4 cut(s) 185, 240, 850, 1052
EarI CTCTTC 4 cut(s) 185, 240, 850, 1052
Ecl136II GAGCTC 2 cut(s) 98, 586
EclXI CGGCCG 1 cut(s) 208
Eco130I CCWWGG 2 cut(s) 558, 717
Eco24I GRGCYC 2 cut(s) 100, 588
Eco47I GGWCC 3 cut(s) 76, 728, 756
Eco52I CGGCCG 1 cut(s) 208
Eco53kI GAGCTC 2 cut(s) 98, 586
EcoICRI GAGCTC 2 cut(s) 98, 586
EcoO109I RGGNCCY 1 cut(s) 756
EcoRII CCWGG 4 cut(s) 663, 699, 871, 950
EcoT14I CCWWGG 2 cut(s) 558, 717
EcoT38I GRGCYC 2 cut(s) 100, 588
ErhI CCWWGG 2 cut(s) 558, 717
Esp3I CGTCTC 1 cut(s) 346
FaeI CATG 7 cut(s) 466, 493, 545, 557, 562, 721, 794
FatI CATG 7 cut(s) 462, 489, 541, 553, 558, 717, 790
FblI GTMKAC 2 cut(s) 669, 825
FokI GGATG 2 cut(s) 464, 479
FriOI GRGCYC 2 cut(s) 100, 588
GlaI GCGC 4 cut(s) 156, 280, 413, 435
GsuI CTGGAG 2 cut(s) 30, 287
HaeIII GGCC 4 cut(s) 210, 306, 709, 925
HapII CCGG 4 cut(s) 93, 211, 217, 636
HgaI GACGC 1 cut(s) 161
HhaI GCGC 4 cut(s) 157, 281, 414, 436
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 7 cut(s) 466, 493, 545, 557, 562, 721, 794
Hin6I GCGC 4 cut(s) 155, 279, 412, 434
HinP1I GCGC 4 cut(s) 155, 279, 412, 434
HincII GTYRAC 2 cut(s) 621, 670
HindII GTYRAC 2 cut(s) 621, 670
HindIII AAGCTT 1 cut(s) 604
HinfI GANTC 1 cut(s) 723
HpaII CCGG 4 cut(s) 93, 211, 217, 636
HphI GGTGA 5 cut(s) 17, 171, 638, 887, 1094
Hpy166II GTNNAC 4 cut(s) 621, 670, 826, 866
Hpy188I TCNGA 5 cut(s) 76, 102, 548, 652, 741
Hpy188III TCNNGA 3 cut(s) 47, 93, 490
Hpy8I GTNNAC 4 cut(s) 621, 670, 826, 866
HpyAV CCTTC 3 cut(s) 76, 529, 623
HpyCH4III ACNGT 3 cut(s) 183, 674, 1031
HpyCH4IV ACGT 3 cut(s) 104, 168, 339
HpyCH4V TGCA 6 cut(s) 204, 403, 535, 961, 976, 1079
HpyF10VI GCNNNNNNNGC 9 cut(s) 201, 204, 240, 400, 409, 440, 563, 572, 775
HpyF3I CTNAG 3 cut(s) 298, 744, 918
HpySE526I ACGT 3 cut(s) 104, 168, 339
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 7 cut(s) 466, 493, 545, 557, 562, 721, 794
HspAI GCGC 4 cut(s) 155, 279, 412, 434
Kpn2I TCCGGA 1 cut(s) 92
Kzo9I GATC 5 cut(s) 354, 370, 384, 589, 987
LguI GCTCTTC 3 cut(s) 185, 850, 1052
LmnI GCTCC 5 cut(s) 95, 103, 521, 583, 766
MabI ACCWGGT 1 cut(s) 663
MaeII ACGT 3 cut(s) 104, 168, 339
MaeIII GTNAC 5 cut(s) 674, 806, 875, 1031, 1082
MalI GATC 5 cut(s) 356, 372, 386, 591, 989
MbiI CCGCTC 1 cut(s) 88
MboI GATC 5 cut(s) 354, 370, 384, 589, 987
MboII GAAGA 7 cut(s) 202, 257, 266, 269, 867, 953, 1069
MfeI CAATTG 2 cut(s) 530, 977
MhlI GDGCHC 2 cut(s) 100, 588
MluCI AATT 5 cut(s) 530, 782, 840, 977, 1021
MmeI TCCRAC 3 cut(s) 125, 675, 764
MroI TCCGGA 1 cut(s) 92
MroXI GAANNNNTTC 1 cut(s) 140
MseI TTAA 2 cut(s) 608, 761
MspA1I CMGCKG 2 cut(s) 207, 376
MspI CCGG 4 cut(s) 93, 211, 217, 636
MspR9I CCNGG 4 cut(s) 665, 701, 873, 952
MunI CAATTG 2 cut(s) 530, 977
MvaI CCWGG 4 cut(s) 665, 701, 873, 952
MvnI CGCG 5 cut(s) 122, 155, 157, 281, 414
MwoI GCNNNNNNNGC 9 cut(s) 201, 204, 240, 400, 409, 440, 563, 572, 775
NcoI CCATGG 2 cut(s) 558, 717
NdeII GATC 5 cut(s) 354, 370, 384, 589, 987
NlaIII CATG 7 cut(s) 466, 493, 545, 557, 562, 721, 794
NlaIV GGNNCC 2 cut(s) 215, 634
NmeAIII GCCGAG 1 cut(s) 405
NmuCI GTSAC 3 cut(s) 674, 875, 1082
NspI RCATGY 1 cut(s) 545
PagI TCATGA 1 cut(s) 489
PaqCI CACCTGC 1 cut(s) 1070
PciI ACATGT 1 cut(s) 541
PciSI GCTCTTC 3 cut(s) 185, 850, 1052
PcsI WCGNNNNNNNCGW 6 cut(s) 159, 168, 363, 396, 666, 732
PdmI GAANNNNTTC 1 cut(s) 140
PfeI GAWTC 1 cut(s) 723
PflFI GACNNNGTC 3 cut(s) 167, 170, 665
PflMI CCANNNNNTGG 1 cut(s) 463
PinAI ACCGGT 1 cut(s) 635
PpuMI RGGWCCY 1 cut(s) 756
PscI ACATGT 1 cut(s) 541
Psp124BI GAGCTC 2 cut(s) 100, 588
Psp5II RGGWCCY 1 cut(s) 756
Psp6I CCWGG 4 cut(s) 663, 699, 871, 950
PspGI CCWGG 4 cut(s) 663, 699, 871, 950
PspN4I GGNNCC 2 cut(s) 215, 634
PspPI GGNCC 5 cut(s) 76, 304, 707, 728, 756
PspPPI RGGWCCY 1 cut(s) 756
PstI CTGCAG 1 cut(s) 206
PstNI CAGNNNCTG 1 cut(s) 201
PsyI GACNNNGTC 3 cut(s) 167, 170, 665
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 2 cut(s) 317, 428
RsaNI GTAC 2 cut(s) 316, 427
SacI GAGCTC 2 cut(s) 100, 588
SalI GTCGAC 1 cut(s) 668
SapI GCTCTTC 3 cut(s) 185, 850, 1052
SaqAI TTAA 2 cut(s) 608, 761
Sau3AI GATC 5 cut(s) 354, 370, 384, 589, 987
Sau96I GGNCC 5 cut(s) 76, 304, 707, 728, 756
ScaI AGTACT 1 cut(s) 428
ScrFI CCNGG 4 cut(s) 665, 701, 873, 952
SduI GDGCHC 2 cut(s) 100, 588
SexAI ACCWGGT 1 cut(s) 663
SfcI CTRYAG 1 cut(s) 202
SgrAI CRCCGGYG 1 cut(s) 635
SinI GGWCC 3 cut(s) 76, 728, 756
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 5 cut(s) 530, 782, 840, 977, 1021
SsiI CCGC 6 cut(s) 83, 86, 120, 207, 231, 293
SstI GAGCTC 2 cut(s) 100, 588
StyD4I CCNGG 4 cut(s) 663, 699, 871, 950
StyI CCWWGG 2 cut(s) 558, 717
TaaI ACNGT 3 cut(s) 183, 674, 1031
TaiI ACGT 3 cut(s) 107, 171, 342
TaqI TCGA 6 cut(s) 127, 369, 387, 439, 588, 669
TaqII GACCGA 1 cut(s) 716
TasI AATT 5 cut(s) 530, 782, 840, 977, 1021
TatI WGTACW 1 cut(s) 426
TauI GCSGC 5 cut(s) 85, 88, 122, 210, 234
TfiI GAWTC 1 cut(s) 723
Tru1I TTAA 2 cut(s) 608, 761
Tru9I TTAA 2 cut(s) 608, 761
TscAI CASTG 2 cut(s) 573, 679
TseFI GTSAC 3 cut(s) 674, 875, 1082
Tsp45I GTSAC 3 cut(s) 674, 875, 1082
TspDTI ATGAA 4 cut(s) 478, 542, 807, 991
TspGWI ACGGA 1 cut(s) 950
TspRI CASTG 2 cut(s) 573, 679
Tth111I GACNNNGTC 3 cut(s) 167, 170, 665
Van91I CCANNNNNTGG 1 cut(s) 463
VpaK11BI GGWCC 3 cut(s) 76, 728, 756
XapI RAATTY 1 cut(s) 840
XceI RCATGY 1 cut(s) 545
XmiI GTMKAC 2 cut(s) 669, 825
XmnI GAANNNNTTC 1 cut(s) 140
ZraI GACGTC 1 cut(s) 169
ZrmI AGTACT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.