pycom01g03020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003663
Physical Location & Seq
Forward (+)
2440748 .. 2441235
488 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03020.2

Sequence Viewer

Length: 396 bp
ATGCGTGCCATTCCGATGTCTGAGAAACCTGGCTTGAAGCAGGTTCTGGAGGACTCGGCAGCTGAGGCGAAGGGAGGATCAACCGATTCGAGGCTGTCCAATTGCGAATGGGTTGGCCAAGGACTCGATGCAATTCGAGACGACGTCGCCGAGCCGGTGAATCAGGAATTAGGGTCGGCGTGCCCGGTGGCAGAATCGGTGGCAGACGGTGATAACCAGGTGGCGGAAGATAAAGGCAAAGTGACGGAAGATTCGGGGAAAGAGGAGTTTGTGGATTGCTCGGAGGATTACGCAATGGATGAGTTGGATCGCCTGCGGCTTTTGCTGGATACTACTGTTGACTGTAATTTTCCCATGTCAAATTACAATTTTACCCATGTATTCACAGATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.32

Weight (kDa)

4.15

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 31
AciI CCGC 2 cut(s) 224, 316
AclWI GGATC 2 cut(s) 85, 315
AcoI YGGCCR 1 cut(s) 115
AcyI GRCGYC 1 cut(s) 144
AfiI CCNNNNNNNGG 2 cut(s) 90, 223
AgsI TTSAA 1 cut(s) 37
AjnI CCWGG 2 cut(s) 28, 216
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 2 cut(s) 85, 315
AlwNI CAGNNNCTG 1 cut(s) 46
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 59
ArsI GACNNNNNNTTYG 2 cut(s) 235, 267
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 169, 221
BaeGI GKGCMC 1 cut(s) 185
BaeI ACNNNNGTAYC 2 cut(s) 321, 354
BalI TGGCCA 1 cut(s) 117
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 71
BciT130I CCWGG 2 cut(s) 30, 218
BciVI GTATCC 1 cut(s) 322
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 132
BfuAI ACCTGC 1 cut(s) 31
BfuI GTATCC 1 cut(s) 322
BisI GCNGC 2 cut(s) 60, 317
BlsI GCNGC 2 cut(s) 61, 318
Bme1390I CCNGG 3 cut(s) 30, 185, 218
BmrFI CCNGG 3 cut(s) 30, 185, 218
BmsI GCATC 1 cut(s) 118
BpmI CTGGAG 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 63
BpuMI CCSGG 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 118
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 223
Bse118I RCCGGY 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 2 cut(s) 30, 218
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 90, 223
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 2 cut(s) 12, 54
BseRI GAGGAG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 1 cut(s) 71
BshFI GGCC 1 cut(s) 117
BsiSI CCGG 2 cut(s) 155, 185
BslI CCNNNNNNNGG 2 cut(s) 90, 223
BsmAI GTCTC 1 cut(s) 132
BsmBI CGTCTC 1 cut(s) 132
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 2 cut(s) 77, 307
BspACI CCGC 2 cut(s) 224, 316
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 13, 55
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 2 cut(s) 85, 315
BsrDI GCAATG 1 cut(s) 300
BsrFI RCCGGY 1 cut(s) 154
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 2 cut(s) 77, 307
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 30, 218
Bst4CI ACNGT 3 cut(s) 209, 337, 344
BstACI GRCGYC 1 cut(s) 144
BstC8I GCNNGC 3 cut(s) 6, 181, 314
BstDEI CTNAG 2 cut(s) 21, 63
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 2 cut(s) 80, 310
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 77, 307
BstMWI GCNNNNNNNGC 2 cut(s) 65, 322
BstNI CCWGG 2 cut(s) 30, 218
BstSCI CCNGG 3 cut(s) 28, 183, 216
BstSLI GKGCMC 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 71
BsuI GTATCC 1 cut(s) 322
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 1 cut(s) 304
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 3 cut(s) 6, 181, 314
CaiI CAGNNNCTG 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 154
CsiI ACCWGGT 1 cut(s) 216
CviAII CATG 2 cut(s) 355, 377
CviJI RGCY 6 cut(s) 33, 62, 94, 117, 154, 319
CviKI_1 RGCY 6 cut(s) 33, 62, 94, 117, 154, 319
DdeI CTNAG 2 cut(s) 21, 63
DpnI GATC 2 cut(s) 79, 309
DpnII GATC 2 cut(s) 77, 307
EaeI YGGCCR 1 cut(s) 115
EciI GGCGGA 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 118
EcoRII CCWGG 2 cut(s) 28, 216
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
Esp3I CGTCTC 1 cut(s) 132
FaeI CATG 2 cut(s) 358, 380
FaiI YATR 2 cut(s) 356, 378
FatI CATG 2 cut(s) 354, 376
Fnu4HI GCNGC 2 cut(s) 60, 317
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 60, 317
GluI GCNGC 2 cut(s) 60, 317
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 155, 185
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 2 cut(s) 358, 380
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 6 cut(s) 53, 86, 123, 160, 194, 251
HpaII CCGG 2 cut(s) 155, 185
HphI GGTGA 2 cut(s) 169, 221
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 3 cut(s) 15, 22, 283
Hpy188III TCNNGA 3 cut(s) 47, 137, 164
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 2 cut(s) 146, 149
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 3 cut(s) 209, 337, 344
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 1 cut(s) 131
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 322
HpyF3I CTNAG 2 cut(s) 21, 63
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 2 cut(s) 358, 380
Kzo9I GATC 2 cut(s) 77, 307
Lsp1109I GCAGC 1 cut(s) 71
LweI GCATC 1 cut(s) 118
MabI ACCWGGT 1 cut(s) 216
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 241
MalI GATC 2 cut(s) 79, 309
MboI GATC 2 cut(s) 77, 307
MboII GAAGA 2 cut(s) 239, 260
MfeI CAATTG 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 185
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 6 cut(s) 100, 132, 167, 346, 361, 367
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 2 cut(s) 47, 117
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 6 cut(s) 43, 58, 68, 84, 256, 277
Mox20I TGGCCA 1 cut(s) 117
MscI TGGCCA 1 cut(s) 117
MslI CAYNNNNRTG 1 cut(s) 14
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 2 cut(s) 155, 185
MspR9I CCNGG 3 cut(s) 30, 185, 218
MunI CAATTG 1 cut(s) 100
MvaI CCWGG 2 cut(s) 30, 218
MwoI GCNNNNNNNGC 2 cut(s) 65, 322
NciI CCSGG 1 cut(s) 185
NdeII GATC 2 cut(s) 77, 307
NlaIII CATG 2 cut(s) 358, 380
NmeAIII GCCGAG 2 cut(s) 35, 175
NmuCI GTSAC 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 4 cut(s) 86, 160, 194, 251
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 2 cut(s) 61, 318
PleI GAGTC 2 cut(s) 47, 117
PpsI GAGTC 2 cut(s) 47, 117
Psp6I CCWGG 2 cut(s) 28, 216
PspGI CCWGG 2 cut(s) 28, 216
PstNI CAGNNNCTG 1 cut(s) 46
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 62
RseI CAYNNNNRTG 1 cut(s) 14
SatI GCNGC 2 cut(s) 60, 317
Sau3AI GATC 2 cut(s) 77, 307
SchI GAGTC 2 cut(s) 47, 117
ScrFI CCNGG 3 cut(s) 30, 185, 218
SduI GDGCHC 1 cut(s) 185
SetI ASST 5 cut(s) 31, 45, 64, 147, 222
SexAI ACCWGGT 1 cut(s) 216
SfaNI GCATC 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 6 cut(s) 100, 132, 167, 346, 361, 367
SsiI CCGC 2 cut(s) 224, 316
StyD4I CCNGG 3 cut(s) 28, 183, 216
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 209, 337, 344
TaiI ACGT 1 cut(s) 147
TaqI TCGA 3 cut(s) 89, 126, 136
TasI AATT 6 cut(s) 100, 132, 167, 346, 361, 367
TauI GCSGC 1 cut(s) 319
TfiI GAWTC 4 cut(s) 86, 160, 194, 251
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 241
TspGWI ACGGA 1 cut(s) 260
Tth111I GACNNNGTC 1 cut(s) 143
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.