Rmu_sc0003919.1_g000022

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003919.1
OG0003663
Physical Location & Seq
Reverse (-)
113373 .. 114069
697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003919.1_g000022.1.cds

Sequence Viewer

Length: 456 bp
atgaaaccgcagaacacacattgggggtattttgctgctgctcgtaacccaatctctcccagggtgaatgcgagacccccggcaacctcatcacgagaaccccgcgagcccccactcgtagatctcgagagtgaacttggacaaacaaaaacggagctggagcaggagaagaataggtgtgctaatactagagagaagcttactatcgctgtgcagagaagtcaagtgcgcgctcttgaagctgctgaattaagcaaggaggaattgattaggagtgaaaactcagttgcatcactgcaagaaacactttcccaaaataatgtaattcttcagaaacttgaagaaatgttgtctcagaccggtgtacctgaagaacttcaatctatggatattgtagagagacttcgatggcttgtggatgaaagtgttaaactcaaggaaaaatctgtggagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

17.18

Weight (kDa)

5.29

Isoelectric Point (pI)

60.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 105, 231
AciI CCGC 2 cut(s) 8, 103
AcuI CTGAAG 2 cut(s) 314, 390
AfaI GTAC 1 cut(s) 366
AgeI ACCGGT 1 cut(s) 359
AgsI TTSAA 3 cut(s) 239, 341, 380
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 3 cut(s) 157, 199, 242
AluI AGCT 3 cut(s) 157, 199, 242
Alw26I GTCTC 3 cut(s) 67, 357, 394
Ama87I CYCGRG 1 cut(s) 125
ApeKI GCWGC 3 cut(s) 35, 38, 242
AsiGI ACCGGT 1 cut(s) 359
Asp700I GAANNNNTTC 1 cut(s) 375
AspLEI GCGC 2 cut(s) 231, 233
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 125
BaeI ACNNNNGTAYC 2 cut(s) 348, 381
BanII GRGCYC 1 cut(s) 111
BarI GAAGNNNNNNTAC 2 cut(s) 387, 419
BauI CACGAG 1 cut(s) 93
BbvI GCAGC 3 cut(s) 22, 25, 229
BccI CCATC 1 cut(s) 402
BciT130I CCWGG 1 cut(s) 61
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 3 cut(s) 67, 357, 394
BfaI CTAG 1 cut(s) 189
BglII AGATCT 1 cut(s) 121
BisI GCNGC 3 cut(s) 36, 39, 243
BlsI GCNGC 3 cut(s) 37, 40, 244
Bme1390I CCNGG 2 cut(s) 61, 80
BmeT110I CYCGRG 1 cut(s) 125
BmrFI CCNGG 2 cut(s) 61, 80
BmsI GCATC 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 80
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 3 cut(s) 59, 60, 78
BsaWI WCCGGW 1 cut(s) 359
Bse118I RCCGGY 1 cut(s) 359
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 3 cut(s) 59, 60, 78
BseGI GGATG 1 cut(s) 424
BseMII CTCAG 2 cut(s) 297, 368
BsePI GCGCGC 1 cut(s) 229
BseXI GCAGC 3 cut(s) 22, 25, 229
BsgI GTGCAG 1 cut(s) 233
Bsh1236I CGCG 2 cut(s) 105, 231
BshTI ACCGGT 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 2 cut(s) 80, 360
BsmAI GTCTC 3 cut(s) 67, 357, 394
BsmI GAATGC 1 cut(s) 73
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 121
BspACI CCGC 2 cut(s) 8, 103
BspCNI CTCAG 2 cut(s) 296, 367
BspFNI CGCG 2 cut(s) 105, 231
BspTNI GGTCTC 1 cut(s) 67
BsrFI RCCGGY 1 cut(s) 359
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 3 cut(s) 59, 60, 78
BssHII GCGCGC 1 cut(s) 229
BssMI GATC 1 cut(s) 121
BssSI CACGAG 1 cut(s) 93
Bst2BI CACGAG 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 61
BstC8I GCNNGC 2 cut(s) 107, 231
BstDEI CTNAG 2 cut(s) 283, 354
BstF5I GGATG 1 cut(s) 424
BstFNI CGCG 2 cut(s) 105, 231
BstHHI GCGC 2 cut(s) 231, 233
BstKTI GATC 1 cut(s) 124
BstMAI GTCTC 3 cut(s) 67, 357, 394
BstMBI GATC 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 61
BstSCI CCNGG 2 cut(s) 59, 78
BstUI CGCG 2 cut(s) 105, 231
BstV1I GCAGC 3 cut(s) 22, 25, 229
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BtsCI GGATG 1 cut(s) 424
BtsI GCAGTG 1 cut(s) 293
BtsIMutI CAGTG 1 cut(s) 293
Cac8I GCNNGC 2 cut(s) 107, 231
CfoI GCGC 2 cut(s) 231, 233
Cfr10I RCCGGY 1 cut(s) 359
Csp6I GTAC 1 cut(s) 365
CspAI ACCGGT 1 cut(s) 359
CviJI RGCY 5 cut(s) 109, 157, 199, 242, 412
CviKI_1 RGCY 5 cut(s) 109, 157, 199, 242, 412
CviQI GTAC 1 cut(s) 365
DdeI CTNAG 2 cut(s) 283, 354
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 314, 390
Eco88I CYCGRG 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 59
EcoT38I GRGCYC 1 cut(s) 111
FaiI YATR 1 cut(s) 386
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FauI CCCGC 1 cut(s) 110
Fnu4HI GCNGC 3 cut(s) 36, 39, 243
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 3 cut(s) 36, 39, 243
FspBI CTAG 1 cut(s) 189
GlaI GCGC 2 cut(s) 230, 232
GluI GCNGC 3 cut(s) 36, 39, 243
GsuI CTGGAG 1 cut(s) 179
HapII CCGG 2 cut(s) 80, 360
HhaI GCGC 2 cut(s) 231, 233
Hin6I GCGC 2 cut(s) 229, 231
HinP1I GCGC 2 cut(s) 229, 231
HindIII AAGCTT 1 cut(s) 197
HpaII CCGG 2 cut(s) 80, 360
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 2 cut(s) 134, 365
Hpy188I TCNGA 2 cut(s) 333, 357
Hpy188III TCNNGA 4 cut(s) 93, 125, 127, 236
Hpy8I GTNNAC 2 cut(s) 134, 365
HpyCH4V TGCA 3 cut(s) 214, 290, 298
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 2 cut(s) 283, 354
HspAI GCGC 2 cut(s) 229, 231
Kzo9I GATC 1 cut(s) 121
LmnI GCTCC 2 cut(s) 154, 160
LpnPI CCDG 7 cut(s) 46, 73, 93, 143, 149, 373, 381
Lsp1109I GCAGC 3 cut(s) 22, 25, 229
LweI GCATC 1 cut(s) 299
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 44
MalI GATC 1 cut(s) 123
MboI GATC 1 cut(s) 121
MboII GAAGA 4 cut(s) 181, 320, 353, 383
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 111
MluCI AATT 3 cut(s) 248, 263, 324
MnlI CCTC 2 cut(s) 97, 253
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 2 cut(s) 251, 429
MspI CCGG 2 cut(s) 80, 360
MspR9I CCNGG 2 cut(s) 61, 80
Mva1269I GAATGC 1 cut(s) 73
MvaI CCWGG 1 cut(s) 61
MvnI CGCG 2 cut(s) 105, 231
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 80
NdeII GATC 1 cut(s) 121
PaeR7I CTCGAG 1 cut(s) 125
PasI CCCWGGG 1 cut(s) 60
PauI GCGCGC 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 375
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 3 cut(s) 37, 40, 244
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PsuI RGATCY 1 cut(s) 121
PteI GCGCGC 1 cut(s) 229
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
SaqAI TTAA 2 cut(s) 251, 429
SatI GCNGC 3 cut(s) 36, 39, 243
Sau3AI GATC 1 cut(s) 121
ScrFI CCNGG 2 cut(s) 61, 80
SduI GDGCHC 1 cut(s) 111
SetI ASST 6 cut(s) 89, 159, 179, 201, 244, 370
SfaNI GCATC 1 cut(s) 299
Sfr274I CTCGAG 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 125, 434
SmoI CTYRAG 2 cut(s) 125, 434
Sse9I AATT 3 cut(s) 248, 263, 324
SsiI CCGC 2 cut(s) 8, 103
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 59, 78
TaqI TCGA 2 cut(s) 126, 406
TasI AATT 3 cut(s) 248, 263, 324
Tru1I TTAA 2 cut(s) 251, 429
Tru9I TTAA 2 cut(s) 251, 429
TscAI CASTG 1 cut(s) 300
TseI GCWGC 3 cut(s) 35, 38, 242
TspDTI ATGAA 2 cut(s) 17, 435
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 1 cut(s) 300
XhoI CTCGAG 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.