pycom01g08870

Glycosyl hydrolase family 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006678
Physical Location & Seq
Reverse (-)
9843093 .. 9845564
2472 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 912 bp
ATGATGATGAAGTCGGTTGATGTTGAAGGTGTTATGGTTGATTGCTGGTGGGGAATTGTAGAGGCACACAGTCGGCAGGTATATAATTGGAGTAGCTACAAGAAACTATTCCAAATTGTGCGCGTTCTTAACCTTAAGTTACAGGTCGTGATGTCATTCCACGAGTGTGGAGGCAATGTCGGGGATGATGTACATATCCCACTTCCTCACTGGGTGACAGAAATTGGCCAAAAGAATCCTGATTTATATTTTACTAATAAAGAAGGGAAACGCAACACTGAATGTCTCACATGGGGAATTGATAAAGAGCGGGTTTTAAGAGGCCGCACTGCTGTTGAGGTTTACTTCGACTACATGAGGAGCTTCAGAGTTGAATTTGACGAGTTTTTTGAGGAATTAATAATATCAGAATTTGAAGTTGGATTAGGTTTGTGTGGAGAGCTACGGTATCCTTCTTATCCTGAACAGCATGGTTGGAAATATCCTGGTATTGGTGAATTCCAGTGTTATGATCGATACTTGATGAAGAATCTGACGCAGGCTGCAGAAGCAAGGGGTCACTCCTTCTGGGCCAGAGTACCAGATAATGCAGGCTCTTATAATTCCCAAACACATGAGACAGGGTTTTTCCATGACGGAGGTGATTATGATAGCTACTATGGCAGATTCTTTCTTAATTGGTACTCCCGTGTTTTGATTGATCATGGTGACTGTGTACTTGCTCTGGCCAATTTAGCTTTTGAAGGCACACGCATTGCTACAAAGGTATCAGGTATCCATTGGTGGTACAAGACTGCCAGCCATCCTGCTGAATTAACTGCTGGATTTTACAACTCCTGCAGTCGATGGGTATGCACCAATTTCTGCAATGTTAAAAAAGCATGTGGCTGCTCTGAATTTCACATGTGTTGA

Protein Analysis

304

Amino Acids

35.15

Weight (kDa)

5.95

Isoelectric Point (pI)

32.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 2 - 280 1.1e-93 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
Acc36I ACCTGC 1 cut(s) 67
AccBSI CCGCTC 1 cut(s) 310
AccII CGCG 1 cut(s) 123
AciI CCGC 2 cut(s) 310, 325
AcoI YGGCCR 2 cut(s) 226, 726
AcsI RAATTY 4 cut(s) 374, 410, 497, 896
AcuI CTGAAG 1 cut(s) 349
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 5 cut(s) 192, 579, 683, 717, 788
AfiI CCNNNNNNNGG 1 cut(s) 491
AflII CTTAAG 1 cut(s) 134
AflIII ACRYGT 1 cut(s) 903
AgsI TTSAA 4 cut(s) 26, 374, 416, 743
AjnI CCWGG 1 cut(s) 484
AjuI GAANNNNNNNTTGG 2 cut(s) 402, 434
AleI CACNNNNGTG 1 cut(s) 165
AluBI AGCT 5 cut(s) 96, 363, 442, 654, 737
AluI AGCT 5 cut(s) 96, 363, 442, 654, 737
Alw26I GTCTC 2 cut(s) 290, 611
AoxI GGCC 4 cut(s) 226, 322, 570, 726
ApeKI GCWGC 2 cut(s) 542, 888
ApoI RAATTY 4 cut(s) 374, 410, 497, 896
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
AseI ATTAAT 1 cut(s) 398
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 1 cut(s) 570
AsuHPI GGTGA 4 cut(s) 226, 506, 653, 719
BaeI ACNNNNGTAYC 4 cut(s) 673, 706, 750, 783
BalI TGGCCA 2 cut(s) 228, 728
BauI CACGAG 1 cut(s) 161
BbvI GCAGC 2 cut(s) 529, 875
BccI CCATC 2 cut(s) 810, 840
BcgI CGANNNNNNTGC 2 cut(s) 834, 868
BciT130I CCWGG 1 cut(s) 486
BciVI GTATCC 2 cut(s) 459, 785
BclI TGATCA 1 cut(s) 700
BcoDI GTCTC 2 cut(s) 290, 611
BfmI CTRYAG 2 cut(s) 543, 838
BfrI CTTAAG 1 cut(s) 134
BfuAI ACCTGC 1 cut(s) 67
BfuI GTATCC 2 cut(s) 459, 785
BisI GCNGC 3 cut(s) 325, 543, 889
BlsI GCNGC 3 cut(s) 326, 544, 890
Bme1390I CCNGG 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 570
BmrFI CCNGG 1 cut(s) 486
BmrI ACTGGG 1 cut(s) 220
BmuI ACTGGG 1 cut(s) 220
Bsa29I ATCGAT 1 cut(s) 514
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse1I ACTGG 2 cut(s) 215, 502
Bse3DI GCAATG 3 cut(s) 181, 753, 874
BseBI CCWGG 1 cut(s) 486
BseCI ATCGAT 1 cut(s) 514
BseGI GGATG 2 cut(s) 190, 802
BseLI CCNNNNNNNGG 1 cut(s) 491
BseMI GCAATG 3 cut(s) 181, 753, 874
BseNI ACTGG 2 cut(s) 215, 502
BseRI GAGGAG 1 cut(s) 373
BseXI GCAGC 2 cut(s) 529, 875
Bsh1236I CGCG 1 cut(s) 123
BshFI GGCC 4 cut(s) 228, 324, 572, 728
BshVI ATCGAT 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 491
BsmAI GTCTC 2 cut(s) 290, 611
BsnI GGCC 4 cut(s) 228, 324, 572, 728
Bsp1407I TGTACA 1 cut(s) 190
Bsp143I GATC 2 cut(s) 511, 700
BspACI CCGC 2 cut(s) 310, 325
BspANI GGCC 4 cut(s) 228, 324, 572, 728
BspDI ATCGAT 1 cut(s) 514
BspFNI CGCG 1 cut(s) 123
BspMAI CTGCAG 2 cut(s) 547, 842
BspMI ACCTGC 1 cut(s) 67
BspTI CTTAAG 1 cut(s) 134
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 3 cut(s) 181, 753, 874
BsrGI TGTACA 1 cut(s) 190
BsrI ACTGG 2 cut(s) 215, 502
BssMI GATC 2 cut(s) 511, 700
BssSI CACGAG 1 cut(s) 161
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 3 cut(s) 71, 447, 713
BstAFI CTTAAG 1 cut(s) 134
BstAUI TGTACA 1 cut(s) 190
BstC8I GCNNGC 3 cut(s) 540, 592, 799
BstF5I GGATG 2 cut(s) 190, 802
BstFNI CGCG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 2 cut(s) 514, 703
BstMAI GTCTC 2 cut(s) 290, 611
BstMBI GATC 2 cut(s) 511, 700
BstMWI GCNNNNNNNGC 3 cut(s) 548, 660, 734
BstNI CCWGG 1 cut(s) 486
BstNSI RCATGY 2 cut(s) 885, 907
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 2 cut(s) 543, 838
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 529, 875
BstXI CCANNNNNNTGG 1 cut(s) 167
Bsu15I ATCGAT 1 cut(s) 514
BsuI GTATCC 2 cut(s) 459, 785
BsuRI GGCC 4 cut(s) 228, 324, 572, 728
BsuTUI ATCGAT 1 cut(s) 514
BtsCI GGATG 2 cut(s) 190, 802
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 4 cut(s) 208, 276, 327, 509
BveI ACCTGC 1 cut(s) 67
Cac8I GCNNGC 3 cut(s) 540, 592, 799
CfoI GCGC 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 570
ClaI ATCGAT 1 cut(s) 514
CseI GACGC 1 cut(s) 544
Csp6I GTAC 5 cut(s) 191, 578, 682, 716, 787
CviAII CATG 8 cut(s) 291, 355, 470, 614, 632, 704, 882, 904
CviQI GTAC 5 cut(s) 191, 578, 682, 716, 787
DpnI GATC 2 cut(s) 513, 702
DpnII GATC 2 cut(s) 511, 700
DraIII CACNNNGTG 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 226, 726
Eco57I CTGAAG 1 cut(s) 349
EcoRI GAATTC 1 cut(s) 497
EcoRII CCWGG 1 cut(s) 484
FaeI CATG 8 cut(s) 294, 358, 473, 617, 635, 707, 885, 907
FatI CATG 8 cut(s) 290, 354, 469, 613, 631, 703, 881, 903
FauI CCCGC 1 cut(s) 303
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 3 cut(s) 325, 543, 889
FokI GGATG 2 cut(s) 197, 789
Fsp4HI GCNGC 3 cut(s) 325, 543, 889
GlaI GCGC 1 cut(s) 122
GluI GCNGC 3 cut(s) 325, 543, 889
HaeIII GGCC 4 cut(s) 228, 324, 572, 728
HgaI GACGC 1 cut(s) 544
HhaI GCGC 1 cut(s) 123
Hin1II CATG 8 cut(s) 294, 358, 473, 617, 635, 707, 885, 907
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HinfI GANTC 3 cut(s) 235, 529, 666
HphI GGTGA 4 cut(s) 226, 506, 653, 719
Hpy166II GTNNAC 2 cut(s) 343, 716
Hpy188I TCNGA 4 cut(s) 368, 409, 534, 895
Hpy188III TCNNGA 3 cut(s) 148, 239, 461
Hpy8I GTNNAC 2 cut(s) 343, 716
HpyAV CCTTC 5 cut(s) 20, 257, 462, 574, 737
HpyCH4III ACNGT 3 cut(s) 71, 447, 713
HpyCH4V TGCA 5 cut(s) 545, 590, 840, 855, 867
HpyF10VI GCNNNNNNNGC 3 cut(s) 548, 660, 734
Hsp92II CATG 8 cut(s) 294, 358, 473, 617, 635, 707, 885, 907
HspAI GCGC 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 2 cut(s) 511, 700
LmnI GCTCC 1 cut(s) 360
Lsp1109I GCAGC 2 cut(s) 529, 875
MaeIII GTNAC 4 cut(s) 138, 214, 557, 707
MalI GATC 2 cut(s) 513, 702
MbiI CCGCTC 1 cut(s) 310
MboI GATC 2 cut(s) 511, 700
MboII GAAGA 1 cut(s) 538
MlsI TGGCCA 2 cut(s) 228, 728
MluNI TGGCCA 2 cut(s) 228, 728
MmeI TCCRAC 2 cut(s) 400, 455
MnlI CCTC 8 cut(s) 55, 164, 216, 314, 331, 351, 385, 632
Mox20I TGGCCA 2 cut(s) 228, 728
MroXI GAANNNNTTC 1 cut(s) 107
MscI TGGCCA 2 cut(s) 228, 728
MseI TTAA 7 cut(s) 129, 135, 317, 398, 675, 815, 873
MslI CAYNNNNRTG 1 cut(s) 165
Msp20I TGGCCA 2 cut(s) 228, 728
MspCI CTTAAG 1 cut(s) 134
MspR9I CCNGG 1 cut(s) 486
MvaI CCWGG 1 cut(s) 486
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 3 cut(s) 548, 660, 734
NdeII GATC 2 cut(s) 511, 700
NlaIII CATG 8 cut(s) 294, 358, 473, 617, 635, 707, 885, 907
NmuCI GTSAC 3 cut(s) 214, 557, 707
NspI RCATGY 2 cut(s) 885, 907
OliI CACNNNNGTG 1 cut(s) 165
PciI ACATGT 1 cut(s) 903
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 3 cut(s) 235, 529, 666
PkrI GCNGC 3 cut(s) 326, 544, 890
PscI ACATGT 1 cut(s) 903
PshBI ATTAAT 1 cut(s) 398
PsiI TTATAA 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 484
PspGI CCWGG 1 cut(s) 484
PspPI GGNCC 1 cut(s) 570
PstI CTGCAG 2 cut(s) 547, 842
RsaI GTAC 5 cut(s) 192, 579, 683, 717, 788
RsaNI GTAC 5 cut(s) 191, 578, 682, 716, 787
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 7 cut(s) 129, 135, 317, 398, 675, 815, 873
SatI GCNGC 3 cut(s) 325, 543, 889
Sau3AI GATC 2 cut(s) 511, 700
Sau96I GGNCC 1 cut(s) 570
ScrFI CCNGG 1 cut(s) 486
SfcI CTRYAG 2 cut(s) 543, 838
SmiMI CAYNNNNRTG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
SsiI CCGC 2 cut(s) 310, 325
StyD4I CCNGG 1 cut(s) 484
TaaI ACNGT 3 cut(s) 71, 447, 713
TaqI TCGA 3 cut(s) 348, 514, 844
TatI WGTACW 2 cut(s) 190, 715
TauI GCSGC 1 cut(s) 327
TfiI GAWTC 3 cut(s) 235, 529, 666
Tru1I TTAA 7 cut(s) 129, 135, 317, 398, 675, 815, 873
Tru9I TTAA 7 cut(s) 129, 135, 317, 398, 675, 815, 873
TscAI CASTG 4 cut(s) 215, 283, 334, 509
TseFI GTSAC 3 cut(s) 214, 557, 707
TseI GCWGC 2 cut(s) 542, 888
Tsp45I GTSAC 3 cut(s) 214, 557, 707
TspDTI ATGAA 2 cut(s) 23, 539
TspGWI ACGGA 1 cut(s) 651
TspRI CASTG 4 cut(s) 215, 283, 334, 509
Vha464I CTTAAG 1 cut(s) 134
VspI ATTAAT 1 cut(s) 398
XapI RAATTY 4 cut(s) 374, 410, 497, 896
XceI RCATGY 2 cut(s) 885, 907
XcmI CCANNNNNNNNNTGG 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.