pycom01g08970

Glycosyl hydrolase family 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006678
Physical Location & Seq
Reverse (-)
9959130 .. 9959982
853 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ATGGAATCAATGCAGGTTTACTTCGACTACATGAGGAGTTTCAGAGTTGAATTTGATGAATTTTTTGAGGAAGGAATAATATCAGAAATTGAAGTTGGATTAGGTCAGTGTGGAGAGCTACGGTATCCTTCTTATCCTGAACAGCATGGTTGGAAATATCCTGGTATTGGTGAATTCCAGTGTTATGATCGATACTTGATGAAGAATCTGACGCAGGCTGCAGAAGCAAGGGGCCACTCCTTCTGGGCCAGAGTACCAGATAATGCAGGCTCTTATAATTCCCAACCACATGAGACAGGGTTTTTTCGTGACGGAGGTGATTATGATAGCTACTATGGCAGATTCTTTCTTAATTGGTACTCCCGTGTTTTGATTGATCATGGTGACCGTGTACTTGCTCTGGCCAATTTAGCTTTTGAAGGCACACGCATTGCTACAAAGGTATCAGGTATCCACTGGTGGTACAAGACTGCCAGCCATCCTGCTGAATTAACTGCTGGATTTTACAACTCCTGCAATCGTGATGGGTATGCTATGCGCCAATTTCTGCAATGTTAA

Protein Analysis

186

Amino Acids

21.59

Weight (kDa)

5.33

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 4 - 178 2e-54 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
Acc36I ACCTGC 1 cut(s) 4
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 3 cut(s) 50, 59, 173
AfaI GTAC 4 cut(s) 255, 359, 393, 464
AfiI CCNNNNNNNGG 1 cut(s) 167
AgsI TTSAA 3 cut(s) 50, 92, 419
AjnI CCWGG 1 cut(s) 160
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 3 cut(s) 118, 330, 413
AluI AGCT 3 cut(s) 118, 330, 413
Alw26I GTCTC 1 cut(s) 287
AoxI GGCC 3 cut(s) 232, 246, 402
ApeKI GCWGC 1 cut(s) 218
ApoI RAATTY 3 cut(s) 50, 59, 173
AspLEI GCGC 1 cut(s) 540
AspS9I GGNCC 2 cut(s) 232, 246
AsuHPI GGTGA 3 cut(s) 182, 329, 395
BaeI ACNNNNGTAYC 4 cut(s) 349, 382, 426, 459
BalI TGGCCA 1 cut(s) 404
BbvI GCAGC 1 cut(s) 205
BccI CCATC 2 cut(s) 486, 518
BciT130I CCWGG 1 cut(s) 162
BciVI GTATCC 2 cut(s) 135, 461
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 287
BfmI CTRYAG 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 4
BfuI GTATCC 2 cut(s) 135, 461
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
Bme1390I CCNGG 1 cut(s) 162
BmgT120I GGNCC 2 cut(s) 232, 246
BmiI GGNNCC 1 cut(s) 233
BmrFI CCNGG 1 cut(s) 162
Bsa29I ATCGAT 1 cut(s) 190
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 178, 461
Bse3DI GCAATG 2 cut(s) 429, 557
BseBI CCWGG 1 cut(s) 162
BseCI ATCGAT 1 cut(s) 190
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMI GCAATG 2 cut(s) 429, 557
BseNI ACTGG 2 cut(s) 178, 461
BseRI GAGGAG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 205
BshFI GGCC 3 cut(s) 234, 248, 404
BshVI ATCGAT 1 cut(s) 190
BslI CCNNNNNNNGG 1 cut(s) 167
BsmAI GTCTC 1 cut(s) 287
BsnI GGCC 3 cut(s) 234, 248, 404
Bsp143I GATC 2 cut(s) 187, 376
BspANI GGCC 3 cut(s) 234, 248, 404
BspDI ATCGAT 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 233
BspMAI CTGCAG 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 4
BsrDI GCAATG 2 cut(s) 429, 557
BsrI ACTGG 2 cut(s) 178, 461
BssMI GATC 2 cut(s) 187, 376
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 123, 389
BstC8I GCNNGC 3 cut(s) 216, 268, 475
BstEII GGTNACC 1 cut(s) 383
BstF5I GGATG 1 cut(s) 478
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 2 cut(s) 190, 379
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 2 cut(s) 187, 376
BstMWI GCNNNNNNNGC 3 cut(s) 224, 336, 410
BstNI CCWGG 1 cut(s) 162
BstPI GGTNACC 1 cut(s) 383
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 205
Bsu15I ATCGAT 1 cut(s) 190
BsuI GTATCC 2 cut(s) 135, 461
BsuRI GGCC 3 cut(s) 234, 248, 404
BsuTUI ATCGAT 1 cut(s) 190
BtsCI GGATG 1 cut(s) 478
BtsIMutI CAGTG 3 cut(s) 113, 185, 454
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 3 cut(s) 216, 268, 475
CfoI GCGC 1 cut(s) 540
Cfr13I GGNCC 2 cut(s) 232, 246
ClaI ATCGAT 1 cut(s) 190
CseI GACGC 1 cut(s) 220
Csp6I GTAC 4 cut(s) 254, 358, 392, 463
CviAII CATG 4 cut(s) 31, 146, 290, 380
CviJI RGCY 9 cut(s) 118, 218, 234, 248, 270, 330, 404, 413, 477
CviKI_1 RGCY 9 cut(s) 118, 218, 234, 248, 270, 330, 404, 413, 477
CviQI GTAC 4 cut(s) 254, 358, 392, 463
DpnI GATC 2 cut(s) 189, 378
DpnII GATC 2 cut(s) 187, 376
EaeI YGGCCR 1 cut(s) 402
Eco91I GGTNACC 1 cut(s) 383
EcoO65I GGTNACC 1 cut(s) 383
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 160
FaeI CATG 4 cut(s) 34, 149, 293, 383
FatI CATG 4 cut(s) 30, 145, 289, 379
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 1 cut(s) 465
Fsp4HI GCNGC 1 cut(s) 219
GlaI GCGC 1 cut(s) 539
GluI GCNGC 1 cut(s) 219
HaeIII GGCC 3 cut(s) 234, 248, 404
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 540
Hin1II CATG 4 cut(s) 34, 149, 293, 383
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HinfI GANTC 3 cut(s) 5, 205, 342
HphI GGTGA 3 cut(s) 182, 329, 395
Hpy166II GTNNAC 2 cut(s) 19, 392
Hpy188I TCNGA 3 cut(s) 44, 85, 210
Hpy188III TCNNGA 3 cut(s) 137, 308, 521
Hpy8I GTNNAC 2 cut(s) 19, 392
HpyAV CCTTC 4 cut(s) 65, 138, 250, 413
HpyCH4III ACNGT 2 cut(s) 123, 389
HpyCH4V TGCA 5 cut(s) 13, 221, 266, 516, 550
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 336, 410
Hsp92II CATG 4 cut(s) 34, 149, 293, 383
HspAI GCGC 1 cut(s) 538
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 2 cut(s) 187, 376
Lsp1109I GCAGC 1 cut(s) 205
MaeIII GTNAC 2 cut(s) 308, 383
MalI GATC 2 cut(s) 189, 378
MboI GATC 2 cut(s) 187, 376
MboII GAAGA 1 cut(s) 214
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 9 cut(s) 50, 59, 87, 173, 277, 352, 406, 488, 542
MluNI TGGCCA 1 cut(s) 404
MmeI TCCRAC 2 cut(s) 76, 131
MnlI CCTC 3 cut(s) 27, 61, 308
Mox20I TGGCCA 1 cut(s) 404
MscI TGGCCA 1 cut(s) 404
MseI TTAA 3 cut(s) 351, 491, 556
Msp20I TGGCCA 1 cut(s) 404
MspR9I CCNGG 1 cut(s) 162
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 3 cut(s) 224, 336, 410
NdeII GATC 2 cut(s) 187, 376
NlaIII CATG 4 cut(s) 34, 149, 293, 383
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 2 cut(s) 308, 383
PfeI GAWTC 3 cut(s) 5, 205, 342
PkrI GCNGC 1 cut(s) 220
PsiI TTATAA 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 160
PspEI GGTNACC 1 cut(s) 383
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 1 cut(s) 233
PspPI GGNCC 2 cut(s) 232, 246
PstI CTGCAG 1 cut(s) 223
RsaI GTAC 4 cut(s) 255, 359, 393, 464
RsaNI GTAC 4 cut(s) 254, 358, 392, 463
SaqAI TTAA 3 cut(s) 351, 491, 556
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 2 cut(s) 187, 376
Sau96I GGNCC 2 cut(s) 232, 246
ScrFI CCNGG 1 cut(s) 162
SetI ASST 8 cut(s) 18, 106, 120, 319, 332, 415, 444, 451
SfcI CTRYAG 1 cut(s) 219
Sse9I AATT 9 cut(s) 50, 59, 87, 173, 277, 352, 406, 488, 542
StyD4I CCNGG 1 cut(s) 160
TaaI ACNGT 2 cut(s) 123, 389
TaqI TCGA 2 cut(s) 24, 190
TasI AATT 9 cut(s) 50, 59, 87, 173, 277, 352, 406, 488, 542
TatI WGTACW 1 cut(s) 391
TfiI GAWTC 3 cut(s) 5, 205, 342
Tru1I TTAA 3 cut(s) 351, 491, 556
Tru9I TTAA 3 cut(s) 351, 491, 556
TscAI CASTG 3 cut(s) 113, 185, 461
TseFI GTSAC 2 cut(s) 308, 383
TseI GCWGC 1 cut(s) 218
Tsp45I GTSAC 2 cut(s) 308, 383
TspDTI ATGAA 2 cut(s) 72, 215
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 3 cut(s) 113, 185, 461
XapI RAATTY 3 cut(s) 50, 59, 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.