pycom01g09010

Glycosyl hydrolase family 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006678
Physical Location & Seq
Reverse (-)
10021480 .. 10026242
4763 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1638 bp
ATGGCACAGTTCTCATCTCCTCAAGTATCCCATAAACTGAGGCTGCCACGCCCACCGGGTGCTTGTTGCAAAGCCACGACAATGGCTTCGTTGTCGTCCAGCGGGAAGACTGCGATAGCTTCGCTGAAGCTCTCTCAGGGTCTTTGGGGCTCGCCGGAGCTCAATTTGTGTCCTTTGAGACTATTGGGGAGTGATCCGTCTCGGAGTTTTGCCGTGGTGAGAGACAGTGCTGAGGGGACTGTGGTGGGTGAAAATGTTGACCACAACAAGGTTGAAGACTCTTCTCCGGAAAAGTTTGAGGAGCGTGATTTTACTGGCACGCCGTATGTTCCTGTATATGTCATGTTACCCTTGGGTGTGATCAATATGAACTCCGAGCTAGTTGAGCAAGAAGCTCTGCGAAATCAGCTACAAGTGTTGAAGTCGGTTGGTGTTGATGGTGTTATGGTTGATTGCTGGTGGGGAATTGTAGAGGCACACAGTCCGCAGGGATATAATTGGAGTGGCTACAAGAAACTATTCCAAATTGTGATCGATCTTAACCTTAAGTTACAGGTCGTGATGTCATTCCACGAGTGTGGAGGCAATGTCGGGGATGATGTACATATCCCATTTCCTCACTGGGTGACAGAAATTGGCCAAAAGAATCCTGATATATATTTTACTAATAAAGAAGGGAAACGCAACACTGAATGTCTCACATGGGGAATTGATAAAGAGCGGGTTTTAAGAGGCCGCACTGCTGTTGAGGTTTACTTCGACTACATGAGGAGTTTCAGAGTTGAATTTGATGAATTTTTTGAGGAAGGAATAATATCAGAAATTGAAGTTGGATTAGGTCCGTGTGGAGAGCTACGGTATCCTTCTTATCCTGAACAGCATGGTTGGAAATATCCTGGTATTGGTGAATTCCAGTGTTATGATCGATACTTGATGAAGAATCTGACTCAGGCTGCAGAAGCAAGGGGCCACTCCTTCTGGGCCAGAGTACCAGATAATGCAGGCTCTTATAATTCCCAACCACATGAGACAGGGTTTTTCCGTGACGGAGGTGATTATGATAGCTACTATGGCAGATTCTTTCTTAATTGGTACTCCCGTGTTTTGATTGATCATGGTGACCGTGTACTTGCTCTGGCCAATTTAGCTTTTGAAGGCACACGCATTGCTACAAAGGTATCAGGTATCCACTGGTGGTACAAGACTGCCAGCCATCCTGCTGAATTAACTGCTGGATTTTACAACTCCTGCAATCGTGATGGGTATGTGCCAATTTCTGCAATGTTAAAAAAGCATGAGGCTGCTCTGAATTTCACATGTGTTGAAATGCGCACATTAGACCAACATGAGGGCTTTCCGGAAGCACTGGCAGACCCCGAGGGATTAGTTTGGCAGGTGCTGAATGCTGCATGGGATGCTAACATTCCAGTTGCTAGTGAGAATGCTCTTACTTGCTTTGACAGAGAAGGCTACAACAAGATATTAGAAAATGCTAAGCCCTGGAACGATCCAGATGGCAGGCATTTATCAGCATTTACCTATCTCAGATTAGGCCCAGTTCTCATTGAGAGGCATAACTTAACGGAGTTCGAACGATTTGTCAAGAGAATGCATGGAGAAGCCGCTCCTGATGTTTAG

Protein Analysis

546

Amino Acids

61.54

Weight (kDa)

5.45

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 113 - 527 1.4e-130 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1011
AarI CACCTGC 1 cut(s) 1384
Acc16I TGCGCA 1 cut(s) 1331
Acc36I ACCTGC 1 cut(s) 1384
AccBSI CCGCTC 2 cut(s) 721, 1625
AccIII TCCGGA 2 cut(s) 286, 1357
AciI CCGC 5 cut(s) 102, 485, 721, 736, 1623
AclWI GGATC 2 cut(s) 188, 1502
AcoI YGGCCR 2 cut(s) 637, 1137
AcsI RAATTY 4 cut(s) 785, 794, 908, 1309
AcuI CTGAAG 1 cut(s) 146
AdeI CACNNNGTG 2 cut(s) 59, 625
AfaI GTAC 5 cut(s) 603, 990, 1094, 1128, 1199
AfiI CCNNNNNNNGG 3 cut(s) 268, 902, 1348
AflII CTTAAG 1 cut(s) 545
AflIII ACRYGT 1 cut(s) 1316
AgsI TTSAA 6 cut(s) 275, 421, 785, 827, 1154, 1325
AjnI CCWGG 2 cut(s) 895, 1499
AjuI GAANNNNNNNTTGG 2 cut(s) 813, 845
AleI CACNNNNGTG 1 cut(s) 576
AluBI AGCT 9 cut(s) 119, 130, 160, 379, 395, 409, 853, 1065, 1148
AluI AGCT 9 cut(s) 119, 130, 160, 379, 395, 409, 853, 1065, 1148
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 5 cut(s) 172, 204, 216, 701, 1022
AlwI GGATC 2 cut(s) 188, 1502
AlwNI CAGNNNCTG 1 cut(s) 1399
Ama87I CYCGRG 1 cut(s) 1376
Aor13HI TCCGGA 2 cut(s) 286, 1357
AoxI GGCC 6 cut(s) 637, 733, 967, 981, 1137, 1552
ApeKI GCWGC 4 cut(s) 43, 953, 1301, 1406
ApoI RAATTY 4 cut(s) 785, 794, 908, 1309
ArsI GACNNNNNNTTYG 4 cut(s) 70, 102, 1584, 1616
Asp700I GAANNNNTTC 1 cut(s) 518
AspLEI GCGC 1 cut(s) 1332
AspS9I GGNCC 4 cut(s) 839, 967, 981, 1553
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 6 cut(s) 229, 260, 637, 917, 1064, 1130
AsuII TTCGAA 1 cut(s) 1590
AvaI CYCGRG 1 cut(s) 1376
AvaII GGWCC 1 cut(s) 839
BaeI ACNNNNGTAYC 4 cut(s) 1084, 1117, 1161, 1194
BalI TGGCCA 2 cut(s) 639, 1139
BanII GRGCYC 2 cut(s) 152, 162
BauI CACGAG 1 cut(s) 572
BbsI GAAGAC 2 cut(s) 113, 282
Bbv12I GWGCWC 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 30, 940, 1288, 1393
BccI CCATC 4 cut(s) 431, 1221, 1253, 1508
BceAI ACGGC 2 cut(s) 197, 307
BciT130I CCWGG 2 cut(s) 897, 1501
BciVI GTATCC 3 cut(s) 37, 870, 1196
BclI TGATCA 2 cut(s) 360, 1111
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 5 cut(s) 172, 204, 216, 701, 1022
BfaI CTAG 2 cut(s) 380, 1434
BfmI CTRYAG 1 cut(s) 954
BfrI CTTAAG 1 cut(s) 545
BfuAI ACCTGC 1 cut(s) 1384
BfuI GTATCC 3 cut(s) 37, 870, 1196
BisI GCNGC 6 cut(s) 44, 736, 954, 1302, 1407, 1623
BlpI GCTNAGC 1 cut(s) 1494
BlsI GCNGC 6 cut(s) 45, 737, 955, 1303, 1408, 1624
Bme1390I CCNGG 3 cut(s) 57, 897, 1501
Bme18I GGWCC 1 cut(s) 839
BmeT110I CYCGRG 1 cut(s) 1376
BmgT120I GGNCC 4 cut(s) 839, 967, 981, 1553
BmiI GGNNCC 1 cut(s) 968
BmrFI CCNGG 3 cut(s) 57, 897, 1501
BmrI ACTGGG 2 cut(s) 631, 1550
BmsI GCATC 1 cut(s) 1405
BmuI ACTGGG 2 cut(s) 631, 1550
BpiI GAAGAC 2 cut(s) 113, 282
Bpu10I CCTNAGC 1 cut(s) 231
Bpu1102I GCTNAGC 1 cut(s) 1494
Bpu14I TTCGAA 1 cut(s) 1590
BpuEI CTTGAG 1 cut(s) 6
BpuMI CCSGG 1 cut(s) 57
Bsa29I ATCGAT 2 cut(s) 534, 925
BsaJI CCNNGG 4 cut(s) 213, 351, 1377, 1499
BsaWI WCCGGW 2 cut(s) 286, 1357
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 902, 1348
Bse1I ACTGG 7 cut(s) 319, 626, 913, 1196, 1371, 1427, 1556
Bse3DI GCAATG 3 cut(s) 592, 1164, 1287
BseAI TCCGGA 2 cut(s) 286, 1357
BseBI CCWGG 2 cut(s) 897, 1501
BseCI ATCGAT 2 cut(s) 534, 925
BseDI CCNNGG 4 cut(s) 213, 351, 1377, 1499
BseGI GGATG 3 cut(s) 601, 1213, 1420
BseLI CCNNNNNNNGG 3 cut(s) 268, 902, 1348
BseMI GCAATG 3 cut(s) 592, 1164, 1287
BseMII CTCAG 5 cut(s) 29, 149, 222, 962, 1558
BseNI ACTGG 7 cut(s) 319, 626, 913, 1196, 1371, 1427, 1556
BseRI GAGGAG 3 cut(s) 9, 314, 784
BseXI GCAGC 4 cut(s) 30, 940, 1288, 1393
BshFI GGCC 6 cut(s) 639, 735, 969, 983, 1139, 1554
BshVI ATCGAT 2 cut(s) 534, 925
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 1376
BsiSI CCGG 4 cut(s) 56, 155, 287, 1358
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 3 cut(s) 268, 902, 1348
BsmAI GTCTC 5 cut(s) 172, 204, 216, 701, 1022
BsmBI CGTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 250
BsmI GAATGC 3 cut(s) 1408, 1447, 1614
BsnI GGCC 6 cut(s) 639, 735, 969, 983, 1139, 1554
BsoBI CYCGRG 1 cut(s) 1376
Bsp119I TTCGAA 1 cut(s) 1590
Bsp1286I GDGCHC 2 cut(s) 152, 162
Bsp13I TCCGGA 2 cut(s) 286, 1357
Bsp1407I TGTACA 1 cut(s) 601
Bsp143I GATC 7 cut(s) 193, 360, 531, 535, 922, 1111, 1507
Bsp1720I GCTNAGC 1 cut(s) 1494
BspACI CCGC 5 cut(s) 102, 485, 721, 736, 1623
BspANI GGCC 6 cut(s) 639, 735, 969, 983, 1139, 1554
BspCNI CTCAG 5 cut(s) 30, 148, 223, 961, 1557
BspDI ATCGAT 2 cut(s) 534, 925
BspEI TCCGGA 2 cut(s) 286, 1357
BspLI GGNNCC 1 cut(s) 968
BspMAI CTGCAG 1 cut(s) 958
BspMI ACCTGC 1 cut(s) 1384
BspPI GGATC 2 cut(s) 188, 1502
BspT104I TTCGAA 1 cut(s) 1590
BspTI CTTAAG 1 cut(s) 545
BsrBI CCGCTC 2 cut(s) 721, 1625
BsrDI GCAATG 3 cut(s) 592, 1164, 1287
BsrGI TGTACA 1 cut(s) 601
BsrI ACTGG 7 cut(s) 319, 626, 913, 1196, 1371, 1427, 1556
BssECI CCNNGG 4 cut(s) 213, 351, 1377, 1499
BssMI GATC 7 cut(s) 193, 360, 531, 535, 922, 1111, 1507
BssSI CACGAG 1 cut(s) 572
BssT1I CCWWGG 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 572
Bst2UI CCWGG 2 cut(s) 897, 1501
Bst4CI ACNGT 6 cut(s) 9, 227, 241, 482, 858, 1124
Bst6I CTCTTC 1 cut(s) 286
BstAFI CTTAAG 1 cut(s) 545
BstAPI GCANNNNNTGC 1 cut(s) 1415
BstAUI TGTACA 1 cut(s) 601
BstBI TTCGAA 1 cut(s) 1590
BstC8I GCNNGC 5 cut(s) 152, 320, 1003, 1210, 1520
BstDEI CTNAG 6 cut(s) 38, 135, 231, 948, 1494, 1544
BstDSI CCRYGG 1 cut(s) 213
BstEII GGTNACC 1 cut(s) 1118
BstF5I GGATG 3 cut(s) 601, 1213, 1420
BstHHI GCGC 1 cut(s) 1332
BstKTI GATC 7 cut(s) 196, 363, 534, 538, 925, 1114, 1510
BstMAI GTCTC 5 cut(s) 172, 204, 216, 701, 1022
BstMBI GATC 7 cut(s) 193, 360, 531, 535, 922, 1111, 1507
BstMWI GCNNNNNNNGC 6 cut(s) 385, 406, 959, 1071, 1145, 1415
BstNI CCWGG 2 cut(s) 897, 1501
BstNSI RCATGY 1 cut(s) 1320
BstPI GGTNACC 1 cut(s) 1118
BstSCI CCNGG 3 cut(s) 55, 895, 1499
BstSFI CTRYAG 1 cut(s) 954
BstV1I GCAGC 4 cut(s) 30, 940, 1288, 1393
BstV2I GAAGAC 2 cut(s) 113, 282
BstXI CCANNNNNNTGG 2 cut(s) 82, 578
Bsu15I ATCGAT 2 cut(s) 534, 925
BsuI GTATCC 3 cut(s) 37, 870, 1196
BsuRI GGCC 6 cut(s) 639, 735, 969, 983, 1139, 1554
BsuTUI ATCGAT 2 cut(s) 534, 925
BtgI CCRYGG 1 cut(s) 213
BtsCI GGATG 3 cut(s) 601, 1213, 1420
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 7 cut(s) 232, 619, 687, 738, 920, 1189, 1364
BveI ACCTGC 1 cut(s) 1384
Cac8I GCNNGC 5 cut(s) 152, 320, 1003, 1210, 1520
CaiI CAGNNNCTG 1 cut(s) 1399
CfoI GCGC 1 cut(s) 1332
Cfr13I GGNCC 4 cut(s) 839, 967, 981, 1553
ClaI ATCGAT 2 cut(s) 534, 925
Csp6I GTAC 5 cut(s) 602, 989, 1093, 1127, 1198
CviQI GTAC 5 cut(s) 602, 989, 1093, 1127, 1198
DdeI CTNAG 6 cut(s) 38, 135, 231, 948, 1494, 1544
DpnI GATC 7 cut(s) 195, 362, 533, 537, 924, 1113, 1509
DpnII GATC 7 cut(s) 193, 360, 531, 535, 922, 1111, 1507
DraIII CACNNNGTG 2 cut(s) 59, 625
EaeI YGGCCR 2 cut(s) 637, 1137
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 2 cut(s) 152, 162
Eco47I GGWCC 1 cut(s) 839
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 146
Eco88I CYCGRG 1 cut(s) 1376
Eco91I GGTNACC 1 cut(s) 1118
EcoICRI GAGCTC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 1118
EcoRI GAATTC 1 cut(s) 908
EcoRII CCWGG 2 cut(s) 895, 1499
EcoT14I CCWWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 1614
EcoT38I GRGCYC 2 cut(s) 152, 162
ErhI CCWWGG 1 cut(s) 351
Esp3I CGTCTC 1 cut(s) 204
FaqI GGGAC 1 cut(s) 250
FauI CCCGC 2 cut(s) 95, 714
FbaI TGATCA 2 cut(s) 360, 1111
Fnu4HI GCNGC 6 cut(s) 44, 736, 954, 1302, 1407, 1623
FokI GGATG 3 cut(s) 608, 1200, 1427
FriOI GRGCYC 2 cut(s) 152, 162
Fsp4HI GCNGC 6 cut(s) 44, 736, 954, 1302, 1407, 1623
FspAI RTGCGCAY 1 cut(s) 1331
FspBI CTAG 2 cut(s) 380, 1434
FspI TGCGCA 1 cut(s) 1331
GlaI GCGC 1 cut(s) 1331
GluI GCNGC 6 cut(s) 44, 736, 954, 1302, 1407, 1623
HaeIII GGCC 6 cut(s) 639, 735, 969, 983, 1139, 1554
HapII CCGG 4 cut(s) 56, 155, 287, 1358
HhaI GCGC 1 cut(s) 1332
Hin6I GCGC 1 cut(s) 1330
HinP1I GCGC 1 cut(s) 1330
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 5 cut(s) 278, 646, 940, 946, 1077
HpaII CCGG 4 cut(s) 56, 155, 287, 1358
HphI GGTGA 6 cut(s) 229, 260, 637, 917, 1064, 1130
Hpy166II GTNNAC 3 cut(s) 259, 754, 1127
Hpy188I TCNGA 7 cut(s) 204, 376, 779, 820, 945, 1308, 1547
Hpy188III TCNNGA 9 cut(s) 287, 559, 650, 872, 1256, 1358, 1511, 1603, 1628
Hpy8I GTNNAC 3 cut(s) 259, 754, 1127
HpyAV CCTTC 6 cut(s) 668, 800, 873, 985, 1148, 1460
HpyCH4III ACNGT 6 cut(s) 9, 227, 241, 482, 858, 1124
HpyCH4V TGCA 7 cut(s) 69, 956, 1001, 1251, 1280, 1409, 1612
HpyF10VI GCNNNNNNNGC 6 cut(s) 385, 406, 959, 1071, 1145, 1415
HpyF3I CTNAG 6 cut(s) 38, 135, 231, 948, 1494, 1544
HspAI GCGC 1 cut(s) 1330
Kpn2I TCCGGA 2 cut(s) 286, 1357
Ksp22I TGATCA 2 cut(s) 360, 1111
Kzo9I GATC 7 cut(s) 193, 360, 531, 535, 922, 1111, 1507
LmnI GCTCC 3 cut(s) 157, 301, 1630
Lsp1109I GCAGC 4 cut(s) 30, 940, 1288, 1393
LweI GCATC 1 cut(s) 1405
MaeI CTAG 2 cut(s) 380, 1434
MaeIII GTNAC 5 cut(s) 345, 549, 625, 1043, 1118
MalI GATC 7 cut(s) 195, 362, 533, 537, 924, 1113, 1509
MbiI CCGCTC 2 cut(s) 721, 1625
MboI GATC 7 cut(s) 193, 360, 531, 535, 922, 1111, 1507
MboII GAAGA 4 cut(s) 118, 273, 287, 949
MhlI GDGCHC 2 cut(s) 152, 162
MlsI TGGCCA 2 cut(s) 639, 1139
MluNI TGGCCA 2 cut(s) 639, 1139
MlyI GAGTC 2 cut(s) 272, 940
MmeI TCCRAC 2 cut(s) 811, 866
Mox20I TGGCCA 2 cut(s) 639, 1139
Mph1103I ATGCAT 1 cut(s) 1614
MroI TCCGGA 2 cut(s) 286, 1357
MroXI GAANNNNTTC 1 cut(s) 518
MscI TGGCCA 2 cut(s) 639, 1139
MseI TTAA 7 cut(s) 540, 546, 728, 1086, 1226, 1286, 1580
MslI CAYNNNNRTG 2 cut(s) 80, 576
Msp20I TGGCCA 2 cut(s) 639, 1139
MspA1I CMGCKG 1 cut(s) 102
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 4 cut(s) 56, 155, 287, 1358
MspR9I CCNGG 3 cut(s) 57, 897, 1501
Mva1269I GAATGC 3 cut(s) 1408, 1447, 1614
MvaI CCWGG 2 cut(s) 897, 1501
MwoI GCNNNNNNNGC 6 cut(s) 385, 406, 959, 1071, 1145, 1415
NciI CCSGG 1 cut(s) 57
NdeII GATC 7 cut(s) 193, 360, 531, 535, 922, 1111, 1507
NlaIV GGNNCC 1 cut(s) 968
NmuCI GTSAC 3 cut(s) 625, 1043, 1118
NsbI TGCGCA 1 cut(s) 1331
NsiI ATGCAT 1 cut(s) 1614
NspI RCATGY 1 cut(s) 1320
NspV TTCGAA 1 cut(s) 1590
OliI CACNNNNGTG 1 cut(s) 576
PaqCI CACCTGC 1 cut(s) 1384
PciI ACATGT 1 cut(s) 1316
PctI GAATGC 3 cut(s) 1408, 1447, 1614
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 3 cut(s) 646, 940, 1077
PkrI GCNGC 6 cut(s) 45, 737, 955, 1303, 1408, 1624
PleI GAGTC 2 cut(s) 272, 940
PpsI GAGTC 2 cut(s) 272, 940
PscI ACATGT 1 cut(s) 1316
PsiI TTATAA 1 cut(s) 1011
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 895, 1499
PspEI GGTNACC 1 cut(s) 1118
PspGI CCWGG 2 cut(s) 895, 1499
PspN4I GGNNCC 1 cut(s) 968
PspPI GGNCC 4 cut(s) 839, 967, 981, 1553
PstI CTGCAG 1 cut(s) 958
PstNI CAGNNNCTG 1 cut(s) 1399
RsaI GTAC 5 cut(s) 603, 990, 1094, 1128, 1199
RsaNI GTAC 5 cut(s) 602, 989, 1093, 1127, 1198
RseI CAYNNNNRTG 2 cut(s) 80, 576
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 7 cut(s) 540, 546, 728, 1086, 1226, 1286, 1580
SatI GCNGC 6 cut(s) 44, 736, 954, 1302, 1407, 1623
Sau3AI GATC 7 cut(s) 193, 360, 531, 535, 922, 1111, 1507
Sau96I GGNCC 4 cut(s) 839, 967, 981, 1553
SchI GAGTC 2 cut(s) 272, 940
ScrFI CCNGG 3 cut(s) 57, 897, 1501
SduI GDGCHC 2 cut(s) 152, 162
SfaNI GCATC 1 cut(s) 1405
SfcI CTRYAG 1 cut(s) 954
SfuI TTCGAA 1 cut(s) 1590
SinI GGWCC 1 cut(s) 839
SmiMI CAYNNNNRTG 2 cut(s) 80, 576
SmlI CTYRAG 2 cut(s) 21, 545
SmoI CTYRAG 2 cut(s) 21, 545
SsiI CCGC 5 cut(s) 102, 485, 721, 736, 1623
SspMI CTAG 2 cut(s) 380, 1434
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 55, 895, 1499
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 6 cut(s) 9, 227, 241, 482, 858, 1124
TaqI TCGA 4 cut(s) 534, 759, 925, 1590
TatI WGTACW 2 cut(s) 601, 1126
TauI GCSGC 2 cut(s) 738, 1625
TfiI GAWTC 3 cut(s) 646, 940, 1077
Tru1I TTAA 7 cut(s) 540, 546, 728, 1086, 1226, 1286, 1580
Tru9I TTAA 7 cut(s) 540, 546, 728, 1086, 1226, 1286, 1580
TscAI CASTG 7 cut(s) 232, 626, 694, 745, 920, 1196, 1371
TseFI GTSAC 3 cut(s) 625, 1043, 1118
TseI GCWGC 4 cut(s) 43, 953, 1301, 1406
Tsp45I GTSAC 3 cut(s) 625, 1043, 1118
TspDTI ATGAA 3 cut(s) 383, 807, 950
TspGWI ACGGA 5 cut(s) 186, 831, 1031, 1062, 1598
TspRI CASTG 7 cut(s) 232, 626, 694, 745, 920, 1196, 1371
Vha464I CTTAAG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 839
XapI RAATTY 4 cut(s) 785, 794, 908, 1309
XceI RCATGY 1 cut(s) 1320
XcmI CCANNNNNNNNNTGG 1 cut(s) 618
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 2 cut(s) 380, 1434
Zsp2I ATGCAT 1 cut(s) 1614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.