pycom01g10850
ERF Family

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX MLL subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003180
Physical Location & Seq
Reverse (-)
11617528 .. 11625197
7670 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3231 bp
ATGATAATCAAACGTCCGGAGAAATTGGAAATGCCGAATCTGAAACGCTGCACGCTCGAAGATCCCGAACCTCAAGCCTTTGAGTTTTCCGATAATCCGAAGAAGCGGAAAACAACTGGCTACGATGGAGCGGGCAATGGCGAGGCGGAGGATTTCAGCAGCGGTTCGGGTTCTTCGCACAACGACGAGCCGTTTTGGCCCGCGAGGGAGGGACCGTCGAATTTAGAGCGGTTGATTAATCAACGAGCAGTTATGGCGAAAGAGAAGATGAATCAACGACAATCTTTGGCTAAAGAGAAAACGGAGAAACCGGCATTGAACCGGAGCTTCGGGACGGTACGGAGGTCGAAGCGGGGGCGCATTCAAATGCCTCCTTCGAGGTTTAGTGATTCAGTTTTTGGTGTAAATAACAGTAGCAAAACTAATTTTGACGAAAGGGCAACGACATTTGATGACGAAGGTTTTCATGAAGTTTTAGTAGAAGAAGAAGATACAGAGGGAATTGAGAAATTGGGTTTTGTGAAAAAGAGCAAGGAGGATATTAACCGGCAAAAGAATTCAAAATTTTCGAAGAAAAAGTCAAAGAAGTATGAATATGTTGGGAAGGAAGAACCAGAAGAAAATGAAGTGGATTGTATAGGGTTTAAGGATTTTGATTGTAGAGGTGATTTTAGTTCAGGGGGCATGGACAGACAGAAAAATGCCAATTGTGCAAAGAAGAAAGAAGTATATAAGCCGCAGGACTTTGCTTTGGGTGACGTTGTTTGGGCTAAGTCTGGGAAGAGCTACCCGGCATGGCCGGCTGTGGTTATCGATCCCATTTTGCAGGCTCCCAAATCAGTTCTGAGCTGCTGCGTTCCAGGTGCCATATGCGTGATGTTCTATGGGTTCTCAATGACGACAAATGGATCACAGAGGGATTATGGGTGGGTGAAGCAGGGAATGATATTCCCCTTTACGCAGTTCTTGGACAGATTTCAGGGTCAGACCCAGTTGTACAAGAGCAAGCCTAGTGATTTTCAGATGGCTATAGAGGAGGCGTTGTTAGCAGAAAATGGATTTCTGGACACAAGCTTCGGGAGTTATACAACTGATCCAGAGGCAAATCCTGCCCAATTTCGAGAGGCTAATGGTTTTTATGAAGATCAGGAATATTACACTCAGAACCAGGAGGCATATCCTAAAGATGCAAAAGCTTGTGACTGTTGTGGTTTGGTTACTCCCTCTAAAGCTATGAGAAGAATGAAGGGCTCAAGTTTGTGCACACATTGTTTCAAGTTAAAAAAATCAAAGCAATATTGTGGCATATGCCTGAAGATATGGCACCACTCTGATGGTGGGAGTTGGGTATGCTGTGATGGTTGTGATGTATGGGTGCATGCCGAGTGTGACAAAATTTCCAGTAAAGTTTTAGAGGACCCAGAGCACACTGATTACTATTGCCCTGCTTGCAAAGCGAAGTCCAAGTGTTGTTCGTCAGGTTTGGTAAAACATCAGCAAAAATCCAGTTCTATGCCCAACTTTCAGAAATCTTCTAATTCAAATGTCCAGAAATCTTCTACACCAAATGTTCAGAAATCTTTTGCGCCAAATGTCCAGAAATCTTTGATTCCTGAGGAGGTATCTGTGGTTTGTAATGGAATGGAAGGAACGTATAATCCAAAACTGCATATCATTGTTTGTAAGTGTGGTTCATGTGGGTCAAGGATGCAGACACCTAGTGAATGGGAAAAGCACACAGGCTGCAGAGCCAAAAAATGGAAGCATAGTGTGAAAGTGAAGGCCACAATGCAACCACTGGAAAAATGGATTGAAGACTATAATAAACATGGCTGGAATCCTGTCAAGTTAGTTAAGCAGCGACTAATTGCTTTATTGCAAGAAAAGTACGAGCCAGTTAATACAAAGTGGACAACGGAAAGATGTGCTGTTTGTAGATGGGTTGAAGATTGGGAGGATAATAAAATTATTATATGCAACAGGTGTCAAATAGCTGTGCACCAAGAATGCTATGGAGCAAATGATGTGCAGGATTTTACATCATGGGTTTGTAGAGCATGTGAAACGCCCGATGTCATTAGACAGTGTTGCCTTTGTCCTGTAAGAGGAGGTGCTTTGAAGCCTACTGATGTTGACACCTTGTGGGTTCATGTCACTTGTGCTTGGTTTTGTCCTGGAGTTGGTTTCTTGGAGCATGACAAAATGGAACCTGCAGTAGGAATCCTTAGAATCCTACCATCTACATTTTTGAAGCGCTGTACAATCTGTAAGCAGATTCATGGTTCCTGCACCAAGTGTCGCAAATGTGAAACTGGTTTTCATATAATGTGTGCATCAAGAGCAGGCTACAGCATGGAAGTGCACAGTCTAGAGAAGAATGGCATACCAATAACAAGAAAGTTGATATATTGCCCATTTCATAGCCCCCCAAATCCAGATGCTGTTGTAGTTGTTCATACTAATTCAGGGGTTTTCGCTGCCAGAGGCTCGCTTCAAAATCAGAAGGGGTGCTTCAGAGTTGCAAGGGCAGTGCCATCAGAGAGCACTGAACTTCCAGAATCAGAACCTTCGACCTCAGAGACTAACGAGTTTGAACCGCTCTCTGCTGCAAGAGGCAGTGCTTTTAAAAGATCAAATTTTAAGGGAGGCGAACGGCTGCCAGTATTCCACCGACCAATGGGGCCAACACGTCATTCCTTGGGCACAATAAATAGCTTGAGTACATTTGAAGAAGCAGAAGATTCTAAAGTTTTTTCCTCCTTCAAGGATCGCCTTTTCTACTTACAGAAAACTGAGAATCATCGTGTTTGTTTTGGTAAATCTGGAATACATGGATGGGGCCTATTTGCTCGTAGGAATTTTGAAGAAGGAGAAATGGTTGTTGAGTATCGCGGAGAGAAGGTGAGGCCGAGCGTTGCAGATATGAGAGAAGCACGATACAGACGTGAAGGCAAAGACTGCTATCTCTTCAAGATCAGTGAGGAAGTAGTAATTGATGCTACAAGCAAGGGAAATATAGCACGCTTAATCAACCATTCGTGCATGCCCAACTGCTATGCAAGGATTATGAGTGTCGGTGATGAAGACAGCCGGATTGTTCTCATTGCTAAGACCAGTGTGGCCACCGGTGAAGAACTAACGTACGATTACTTGTTTGATCCGGATGAACTTGATGAGCTGAAAGTTCCGTGCCTCTGTAAAGCACCCAACTGTCGAAAATTTATGAATTAA

Protein Analysis

1077

Amino Acids

121.54

Weight (kDa)

8.37

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 252 - 350 1.4e-15 PWWP domain
PHD PF00628 434 - 484 1.2e-06 PHD-finger
PHD_2 PF13831 654 - 688 1.6e-12 PHD-finger
zf-HC5HC2H_2 PF13832 695 - 807 5.7e-26 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 717 - 808 1.1e-11 PHD-like zinc-binding domain
SET PF00856 945 - 1050 1.4e-24 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2218
AccB1I GGYRCC 2 cut(s) 863, 1323
AccB7I CCANNNNNTGG 1 cut(s) 1758
AccBSI CCGCTC 3 cut(s) 131, 229, 2599
AccII CGCG 2 cut(s) 203, 2892
AccIII TCCGGA 2 cut(s) 16, 3160
AclWI GGATC 6 cut(s) 56, 809, 916, 1088, 2775, 3152
AcoI YGGCCR 2 cut(s) 797, 3120
AcsI RAATTY 7 cut(s) 220, 556, 563, 1395, 2635, 2857, 3218
AcuI CTGAAG 2 cut(s) 1334, 2497
AdeI CACNNNGTG 3 cut(s) 1721, 2142, 2295
AfaI GTAC 6 cut(s) 339, 998, 1889, 2260, 2722, 3143
AfeI AGCGCT 1 cut(s) 2255
AfiI CCNNNNNNNGG 5 cut(s) 1758, 2105, 2180, 2216, 2677
AflIII ACRYGT 1 cut(s) 2687
AgeI ACCGGT 1 cut(s) 3125
AjiI CACGTC 2 cut(s) 2690, 2945
AjnI CCWGG 3 cut(s) 859, 1167, 2173
AjuI GAANNNNNNNTTGG 2 cut(s) 2677, 2709
AloI GAACNNNNNNTCC 2 cut(s) 1642, 1674
AluBI AGCT 9 cut(s) 327, 786, 849, 1074, 1196, 1232, 1994, 2715, 3178
AluI AGCT 9 cut(s) 327, 786, 849, 1074, 1196, 1232, 1994, 2715, 3178
Alw21I GWGCWC 5 cut(s) 1265, 1428, 2001, 2364, 2546
Alw26I GTCTC 1 cut(s) 2573
Alw44I GTGCAC 3 cut(s) 1261, 1997, 2360
AlwI GGATC 6 cut(s) 56, 809, 916, 1088, 2775, 3152
AlwNI CAGNNNCTG 1 cut(s) 2441
Aor13HI TCCGGA 2 cut(s) 16, 3160
Aor51HI AGCGCT 1 cut(s) 2255
AoxI GGCC 7 cut(s) 197, 797, 1782, 2681, 2839, 2906, 3120
ApaLI GTGCAC 3 cut(s) 1261, 1997, 2360
ApeKI GCWGC 9 cut(s) 48, 159, 849, 852, 1743, 1858, 2477, 2606, 2656
ApoI RAATTY 7 cut(s) 220, 556, 563, 1395, 2635, 2857, 3218
ArsI GACNNNNNNTTYG 4 cut(s) 563, 595, 1058, 1090
AseI ATTAAT 1 cut(s) 237
AsiGI ACCGGT 1 cut(s) 3125
Asp700I GAANNNNTTC 1 cut(s) 462
AspLEI GCGC 3 cut(s) 360, 1588, 2256
AspS9I GGNCC 5 cut(s) 198, 212, 1417, 2681, 2839
AsuC2I CCSGG 1 cut(s) 791
AsuHPI GGTGA 6 cut(s) 677, 767, 943, 2914, 3089, 3140
AsuII TTCGAA 1 cut(s) 569
AvaII GGWCC 2 cut(s) 212, 1417
AxyI CCTNAGG 1 cut(s) 1614
BaeGI GKGCMC 4 cut(s) 1265, 2001, 2364, 2705
BaeI ACNNNNGTAYC 4 cut(s) 329, 362, 2929, 2962
BalI TGGCCA 1 cut(s) 3122
BanI GGYRCC 2 cut(s) 863, 1323
BanII GRGCYC 1 cut(s) 1253
BbsI GAAGAC 2 cut(s) 1821, 3090
Bbv12I GWGCWC 5 cut(s) 1265, 1428, 2001, 2364, 2546
BbvI GCAGC 9 cut(s) 35, 171, 836, 839, 1730, 1870, 2464, 2593, 2643
BccI CCATC 8 cut(s) 119, 1018, 1328, 1352, 1932, 2245, 2542, 2829
BceAI ACGGC 2 cut(s) 175, 2669
BciT130I CCWGG 3 cut(s) 861, 1169, 2175
BcnI CCSGG 1 cut(s) 791
BcoDI GTCTC 1 cut(s) 2573
BfaI CTAG 3 cut(s) 1011, 1719, 2369
BfmI CTRYAG 4 cut(s) 1029, 1744, 2211, 2347
BfoI RGCGCY 1 cut(s) 2257
BfuAI ACCTGC 1 cut(s) 2218
BglI GCCNNNNNGGC 1 cut(s) 196
Bme1390I CCNGG 4 cut(s) 791, 861, 1169, 2175
Bme18I GGWCC 2 cut(s) 212, 1417
BmgBI CACGTC 2 cut(s) 2690, 2945
BmgT120I GGNCC 5 cut(s) 198, 212, 1417, 2681, 2839
BmiI GGNNCC 9 cut(s) 213, 831, 865, 1325, 1419, 2208, 2284, 2682, 2840
BmrFI CCNGG 4 cut(s) 791, 861, 1169, 2175
BmrI ACTGGG 1 cut(s) 985
BmsI GCATC 5 cut(s) 1177, 1698, 2342, 2428, 2986
BmuI ACTGGG 1 cut(s) 985
BpiI GAAGAC 2 cut(s) 1821, 3090
BpmI CTGGAG 1 cut(s) 2196
Bpu14I TTCGAA 1 cut(s) 569
BpuEI CTTGAG 3 cut(s) 57, 1237, 2737
BpuMI CCSGG 1 cut(s) 791
Bsa29I ATCGAT 1 cut(s) 813
BsaJI CCNNGG 1 cut(s) 2697
BsaWI WCCGGW 4 cut(s) 16, 321, 3125, 3160
BsaXI ACNNNNNCTCC 8 cut(s) 200, 230, 1028, 1058, 2862, 2886, 2892, 2916
Bsc4I CCNNNNNNNGG 5 cut(s) 1758, 2105, 2180, 2216, 2677
Bse118I RCCGGY 4 cut(s) 310, 546, 799, 3125
Bse1I ACTGG 9 cut(s) 121, 991, 1401, 1506, 1803, 1895, 2317, 2660, 3114
Bse21I CCTNAGG 1 cut(s) 1614
Bse3DI GCAATG 2 cut(s) 142, 3102
BseAI TCCGGA 2 cut(s) 16, 3160
BseBI CCWGG 3 cut(s) 861, 1169, 2175
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 1 cut(s) 2697
BseGI GGATG 3 cut(s) 1713, 2840, 3169
BseLI CCNNNNNNNGG 5 cut(s) 1758, 2105, 2180, 2216, 2677
BseMI GCAATG 2 cut(s) 142, 3102
BseMII CTCAG 5 cut(s) 836, 1175, 1605, 2589, 2784
BseNI ACTGG 9 cut(s) 121, 991, 1401, 1506, 1803, 1895, 2317, 2660, 3114
BseRI GAGGAG 3 cut(s) 1049, 1631, 2121
BseSI GKGCMC 4 cut(s) 1265, 2001, 2364, 2705
BseXI GCAGC 9 cut(s) 35, 171, 836, 839, 1730, 1870, 2464, 2593, 2643
BsgI GTGCAG 3 cut(s) 34, 2048, 2272
Bsh1236I CGCG 2 cut(s) 203, 2892
BshFI GGCC 7 cut(s) 199, 799, 1784, 2683, 2841, 2908, 3122
BshNI GGYRCC 2 cut(s) 863, 1323
BshTI ACCGGT 1 cut(s) 3125
BshVI ATCGAT 1 cut(s) 813
BsiHKAI GWGCWC 5 cut(s) 1265, 1428, 2001, 2364, 2546
BsiSI CCGG 9 cut(s) 17, 311, 322, 547, 791, 800, 3091, 3126, 3161
BsiWI CGTACG 1 cut(s) 3141
BslFI GGGAC 2 cut(s) 225, 346
BslI CCNNNNNNNGG 5 cut(s) 1758, 2105, 2180, 2216, 2677
BsmAI GTCTC 1 cut(s) 2573
BsmFI GGGAC 2 cut(s) 225, 346
BsmI GAATGC 2 cut(s) 360, 2012
BsnI GGCC 7 cut(s) 199, 799, 1784, 2683, 2841, 2908, 3122
Bsp119I TTCGAA 1 cut(s) 569
Bsp1286I GDGCHC 7 cut(s) 1253, 1265, 1428, 2001, 2364, 2546, 2705
Bsp13I TCCGGA 2 cut(s) 16, 3160
Bsp1407I TGTACA 2 cut(s) 996, 2258
Bsp143I GATC 9 cut(s) 61, 814, 908, 1093, 1144, 2630, 2767, 2973, 3157
BspANI GGCC 7 cut(s) 199, 799, 1784, 2683, 2841, 2908, 3122
BspCNI CTCAG 5 cut(s) 837, 1174, 1606, 2588, 2785
BspDI ATCGAT 1 cut(s) 813
BspEI TCCGGA 2 cut(s) 16, 3160
BspFNI CGCG 2 cut(s) 203, 2892
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 9 cut(s) 213, 831, 865, 1325, 1419, 2208, 2284, 2682, 2840
BspMAI CTGCAG 2 cut(s) 1748, 2215
BspMI ACCTGC 1 cut(s) 2218
BspPI GGATC 6 cut(s) 56, 809, 916, 1088, 2775, 3152
BspQI GCTCTTC 1 cut(s) 776
BspT104I TTCGAA 1 cut(s) 569
BspT107I GGYRCC 2 cut(s) 863, 1323
BsrBI CCGCTC 3 cut(s) 131, 229, 2599
BsrDI GCAATG 2 cut(s) 142, 3102
BsrFI RCCGGY 4 cut(s) 310, 546, 799, 3125
BsrGI TGTACA 2 cut(s) 996, 2258
BsrI ACTGG 9 cut(s) 121, 991, 1401, 1506, 1803, 1895, 2317, 2660, 3114
BssAI RCCGGY 4 cut(s) 310, 546, 799, 3125
BssECI CCNNGG 1 cut(s) 2697
BssMI GATC 9 cut(s) 61, 814, 908, 1093, 1144, 2630, 2767, 2973, 3157
BssT1I CCWWGG 1 cut(s) 2697
Bst2UI CCWGG 3 cut(s) 861, 1169, 2175
Bst4CI ACNGT 7 cut(s) 216, 337, 413, 1205, 2085, 2366, 3212
Bst6I CTCTTC 2 cut(s) 776, 2972
BstAPI GCANNNNNTGC 2 cut(s) 1109, 2958
BstAUI TGTACA 2 cut(s) 996, 2258
BstBI TTCGAA 1 cut(s) 569
BstDEI CTNAG 8 cut(s) 771, 845, 1161, 1614, 2225, 2575, 2793, 3108
BstENI CCTNNNNNAGG 2 cut(s) 2103, 2214
BstF5I GGATG 3 cut(s) 1713, 2840, 3169
BstFNI CGCG 2 cut(s) 203, 2892
BstH2I RGCGCY 1 cut(s) 2257
BstHHI GCGC 3 cut(s) 360, 1588, 2256
BstKTI GATC 9 cut(s) 64, 817, 911, 1096, 1147, 2633, 2770, 2976, 3160
BstMAI GTCTC 1 cut(s) 2573
BstMBI GATC 9 cut(s) 61, 814, 908, 1093, 1144, 2630, 2767, 2973, 3157
BstNI CCWGG 3 cut(s) 861, 1169, 2175
BstNSI RCATGY 3 cut(s) 1382, 2061, 3046
BstSCI CCNGG 4 cut(s) 789, 859, 1167, 2173
BstSFI CTRYAG 4 cut(s) 1029, 1744, 2211, 2347
BstSLI GKGCMC 4 cut(s) 1265, 2001, 2364, 2705
BstUI CGCG 2 cut(s) 203, 2892
BstV1I GCAGC 9 cut(s) 35, 171, 836, 839, 1730, 1870, 2464, 2593, 2643
BstV2I GAAGAC 2 cut(s) 1821, 3090
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 1334
BstYI RGATCY 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 813
Bsu36I CCTNAGG 1 cut(s) 1614
BsuRI GGCC 7 cut(s) 199, 799, 1784, 2683, 2841, 2908, 3122
BsuTUI ATCGAT 1 cut(s) 813
BtrI CACGTC 2 cut(s) 2690, 2945
BtsCI GGATG 3 cut(s) 1713, 2840, 3169
BtsI GCAGTG 2 cut(s) 2535, 2623
BtsIMutI CAGTG 8 cut(s) 1428, 1796, 2090, 2535, 2544, 2623, 2983, 3121
BveI ACCTGC 1 cut(s) 2218
CaiI CAGNNNCTG 1 cut(s) 2441
CciI TCATGA 1 cut(s) 466
CfoI GCGC 3 cut(s) 360, 1588, 2256
Cfr10I RCCGGY 4 cut(s) 310, 546, 799, 3125
Cfr13I GGNCC 5 cut(s) 198, 212, 1417, 2681, 2839
ClaI ATCGAT 1 cut(s) 813
Csp6I GTAC 6 cut(s) 338, 997, 1888, 2259, 2721, 3142
CspAI ACCGGT 1 cut(s) 3125
CviQI GTAC 6 cut(s) 338, 997, 1888, 2259, 2721, 3142
DdeI CTNAG 8 cut(s) 771, 845, 1161, 1614, 2225, 2575, 2793, 3108
DpnI GATC 9 cut(s) 63, 816, 910, 1095, 1146, 2632, 2769, 2975, 3159
DpnII GATC 9 cut(s) 61, 814, 908, 1093, 1144, 2630, 2767, 2973, 3157
DraI TTTAAA 1 cut(s) 2626
DraIII CACNNNGTG 3 cut(s) 1721, 2142, 2295
EaeI YGGCCR 2 cut(s) 797, 3120
Eam1104I CTCTTC 2 cut(s) 776, 2972
EarI CTCTTC 2 cut(s) 776, 2972
EciI GGCGGA 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 2697
Eco24I GRGCYC 1 cut(s) 1253
Eco47I GGWCC 2 cut(s) 212, 1417
Eco47III AGCGCT 1 cut(s) 2255
Eco57I CTGAAG 2 cut(s) 1334, 2497
Eco81I CCTNAGG 1 cut(s) 1614
EcoNI CCTNNNNNAGG 2 cut(s) 2103, 2214
EcoO109I RGGNCCY 2 cut(s) 1417, 2839
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 3 cut(s) 859, 1167, 2173
EcoT14I CCWWGG 1 cut(s) 2697
EcoT38I GRGCYC 1 cut(s) 1253
ErhI CCWWGG 1 cut(s) 2697
FalI AAGNNNNNCTT 2 cut(s) 2495, 2527
FaqI GGGAC 2 cut(s) 225, 346
FauI CCCGC 3 cut(s) 124, 208, 345
FauNDI CATATG 2 cut(s) 869, 1307
FokI GGATG 3 cut(s) 1720, 2847, 3176
FriOI GRGCYC 1 cut(s) 1253
FspBI CTAG 3 cut(s) 1011, 1719, 2369
GlaI GCGC 3 cut(s) 359, 1587, 2255
GsuI CTGGAG 1 cut(s) 2196
HaeII RGCGCY 1 cut(s) 2257
HaeIII GGCC 7 cut(s) 199, 799, 1784, 2683, 2841, 2908, 3122
HapII CCGG 9 cut(s) 17, 311, 322, 547, 791, 800, 3091, 3126, 3161
HhaI GCGC 3 cut(s) 360, 1588, 2256
Hin6I GCGC 3 cut(s) 358, 1586, 2254
HinP1I GCGC 3 cut(s) 358, 1586, 2254
HincII GTYRAC 1 cut(s) 2134
HindII GTYRAC 1 cut(s) 2134
HindIII AAGCTT 2 cut(s) 1072, 1194
HpaII CCGG 9 cut(s) 17, 311, 322, 547, 791, 800, 3091, 3126, 3161
HphI GGTGA 6 cut(s) 677, 767, 943, 2914, 3089, 3140
Hpy166II GTNNAC 5 cut(s) 1263, 1911, 1999, 2134, 2362
Hpy8I GTNNAC 5 cut(s) 1263, 1911, 1999, 2134, 2362
Hpy99I CGWCG 2 cut(s) 188, 220
HpyCH4III ACNGT 7 cut(s) 216, 337, 413, 1205, 2085, 2366, 3212
HpyCH4IV ACGT 6 cut(s) 13, 759, 1652, 2689, 2944, 3140
HpyF3I CTNAG 8 cut(s) 771, 845, 1161, 1614, 2225, 2575, 2793, 3108
HpySE526I ACGT 6 cut(s) 13, 759, 1652, 2689, 2944, 3140
HspAI GCGC 3 cut(s) 358, 1586, 2254
Kpn2I TCCGGA 2 cut(s) 16, 3160
KroI GCCGGC 1 cut(s) 799
KroNI GCCGGC 1 cut(s) 801
Kzo9I GATC 9 cut(s) 61, 814, 908, 1093, 1144, 2630, 2767, 2973, 3157
LguI GCTCTTC 1 cut(s) 776
LmnI GCTCC 5 cut(s) 128, 324, 835, 2015, 2191
Lsp1109I GCAGC 9 cut(s) 35, 171, 836, 839, 1730, 1870, 2464, 2593, 2643
LweI GCATC 5 cut(s) 1177, 1698, 2342, 2428, 2986
MaeI CTAG 3 cut(s) 1011, 1719, 2369
MaeII ACGT 6 cut(s) 13, 759, 1652, 2689, 2944, 3140
MaeIII GTNAC 5 cut(s) 755, 1199, 1216, 1388, 2152
MalI GATC 9 cut(s) 63, 816, 910, 1095, 1146, 2632, 2769, 2975, 3159
MbiI CCGCTC 3 cut(s) 131, 229, 2599
MboI GATC 9 cut(s) 61, 814, 908, 1093, 1144, 2630, 2767, 2973, 3157
MfeI CAATTG 1 cut(s) 706
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 7 cut(s) 1253, 1265, 1428, 2001, 2364, 2546, 2705
MlsI TGGCCA 1 cut(s) 3122
MluNI TGGCCA 1 cut(s) 3122
Mox20I TGGCCA 1 cut(s) 3122
MroI TCCGGA 2 cut(s) 16, 3160
MroNI GCCGGC 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 462
MscI TGGCCA 1 cut(s) 3122
MslI CAYNNNNRTG 4 cut(s) 365, 872, 1332, 2357
Msp20I TGGCCA 1 cut(s) 3122
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 9 cut(s) 17, 311, 322, 547, 791, 800, 3091, 3126, 3161
MspR9I CCNGG 4 cut(s) 791, 861, 1169, 2175
MunI CAATTG 1 cut(s) 706
Mva1269I GAATGC 2 cut(s) 360, 2012
MvaI CCWGG 3 cut(s) 861, 1169, 2175
MvnI CGCG 2 cut(s) 203, 2892
NaeI GCCGGC 1 cut(s) 801
NciI CCSGG 1 cut(s) 791
NdeI CATATG 2 cut(s) 869, 1307
NdeII GATC 9 cut(s) 61, 814, 908, 1093, 1144, 2630, 2767, 2973, 3157
NgoMIV GCCGGC 1 cut(s) 799
NlaIV GGNNCC 9 cut(s) 213, 831, 865, 1325, 1419, 2208, 2284, 2682, 2840
NmeAIII GCCGAG 2 cut(s) 1408, 2934
NmuCI GTSAC 4 cut(s) 755, 1199, 1388, 2152
NspI RCATGY 3 cut(s) 1382, 2061, 3046
NspV TTCGAA 1 cut(s) 569
PaeI GCATGC 2 cut(s) 1382, 3046
PagI TCATGA 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 776
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 2941
PctI GAATGC 2 cut(s) 360, 2012
PdiI GCCGGC 1 cut(s) 801
PdmI GAANNNNTTC 1 cut(s) 462
Pfl23II CGTACG 1 cut(s) 3141
PflMI CCANNNNNTGG 1 cut(s) 1758
PfoI TCCNGGA 1 cut(s) 2173
PinAI ACCGGT 1 cut(s) 3125
PpuMI RGGWCCY 1 cut(s) 1417
PshBI ATTAAT 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 1417
Psp6I CCWGG 3 cut(s) 859, 1167, 2173
PspGI CCWGG 3 cut(s) 859, 1167, 2173
PspLI CGTACG 1 cut(s) 3141
PspN4I GGNNCC 9 cut(s) 213, 831, 865, 1325, 1419, 2208, 2284, 2682, 2840
PspPI GGNCC 5 cut(s) 198, 212, 1417, 2681, 2839
PspPPI RGGWCCY 1 cut(s) 1417
PsrI GAACNNNNNNTAC 2 cut(s) 1675, 1707
PstI CTGCAG 2 cut(s) 1748, 2215
PstNI CAGNNNCTG 1 cut(s) 2441
PsuI RGATCY 1 cut(s) 61
RsaI GTAC 6 cut(s) 339, 998, 1889, 2260, 2722, 3143
RsaNI GTAC 6 cut(s) 338, 997, 1888, 2259, 2721, 3142
RseI CAYNNNNRTG 4 cut(s) 365, 872, 1332, 2357
SapI GCTCTTC 1 cut(s) 776
Sau3AI GATC 9 cut(s) 61, 814, 908, 1093, 1144, 2630, 2767, 2973, 3157
Sau96I GGNCC 5 cut(s) 198, 212, 1417, 2681, 2839
ScrFI CCNGG 4 cut(s) 791, 861, 1169, 2175
SduI GDGCHC 7 cut(s) 1253, 1265, 1428, 2001, 2364, 2546, 2705
SfaNI GCATC 5 cut(s) 1177, 1698, 2342, 2428, 2986
SfcI CTRYAG 4 cut(s) 1029, 1744, 2211, 2347
SfuI TTCGAA 1 cut(s) 569
SgrAI CRCCGGYG 1 cut(s) 3125
SinI GGWCC 2 cut(s) 212, 1417
SmiMI CAYNNNNRTG 4 cut(s) 365, 872, 1332, 2357
SmlI CTYRAG 3 cut(s) 72, 1252, 2716
SmoI CTYRAG 3 cut(s) 72, 1252, 2716
SphI GCATGC 2 cut(s) 1382, 3046
SspI AATATT 2 cut(s) 1154, 1298
SspMI CTAG 3 cut(s) 1011, 1719, 2369
StyD4I CCNGG 4 cut(s) 789, 859, 1167, 2173
StyI CCWWGG 1 cut(s) 2697
TaaI ACNGT 7 cut(s) 216, 337, 413, 1205, 2085, 2366, 3212
TaiI ACGT 6 cut(s) 16, 762, 1655, 2692, 2947, 3143
TaqI TCGA 9 cut(s) 57, 218, 347, 377, 569, 813, 1120, 2570, 3214
TatI WGTACW 3 cut(s) 996, 2258, 2720
TauI GCSGC 1 cut(s) 739
TscAI CASTG 8 cut(s) 1435, 1803, 2090, 2535, 2551, 2623, 2983, 3121
TseFI GTSAC 4 cut(s) 755, 1199, 1388, 2152
TseI GCWGC 9 cut(s) 48, 159, 849, 852, 1743, 1858, 2477, 2606, 2656
Tsp45I GTSAC 4 cut(s) 755, 1199, 1388, 2152
TspGWI ACGGA 4 cut(s) 317, 355, 1931, 3177
TspRI CASTG 8 cut(s) 1435, 1803, 2090, 2535, 2551, 2623, 2983, 3121
Van91I CCANNNNNTGG 1 cut(s) 1758
VneI GTGCAC 3 cut(s) 1261, 1997, 2360
VpaK11BI GGWCC 2 cut(s) 212, 1417
VspI ATTAAT 1 cut(s) 237
XagI CCTNNNNNAGG 2 cut(s) 2103, 2214
XapI RAATTY 7 cut(s) 220, 556, 563, 1395, 2635, 2857, 3218
XbaI TCTAGA 1 cut(s) 2368
XceI RCATGY 3 cut(s) 1382, 2061, 3046
XcmI CCANNNNNNNNNTGG 3 cut(s) 1334, 1803, 2009
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 3 cut(s) 1011, 1719, 2369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.