Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0010310

Physical Location & Seq

Genomic Coordinates Reverse (-)

12559735 .. 12567397

Gene Structure 7663 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5100 bp

ATGGAGGAGGCTAGGGTTTCTGGGGACACTGGGAATGGTGCTCCCAGTGAGGAGGCGGTTCGGCCTTCGTACTGGCTGGATGCTTGTGAGGACATACCGTGTGATGTGATTGGTGATTTCGTGGAGTTCTGTGAACCTCCAGTTTGTGGTCCTCCTCTGGTGGCGGCGGTGGCCAATGGCAATGGCTGCAACCAGGAGGAGGATGGGTTGGTGGGTGATTTTTTCGGGGGGATTGATCACATTCTTGACAGTATCAAGAGCGGTGCTGGCCTCCCTGGTACGACTGGTCCGAATTCAGATGTGAATGCCAATGGTGGTACTGGCATTGCTGTTGTTGAGGGTTGCGATAAGATTGAGCCGCCGGTGGTTGTGAATAGGGAGAATGCTGATGACTCGGTTGGACCCAATGTTCGGAGTGGAGGGAGGAGTTTGGAGGCTGATAATGGAGAGAGTAAGGGTGATCGGAATGGTTATCAAAGGTTTGAAAAGAGGAGGGGTAATGGGGTTGTGAGGGAGAGAGATATGGATGGTGAAGAGAGGTTCTCCAAGAGGGTTGCTTTGGATAACGGAAGGAATGCGAGGTATGGTTCAGGCAGAGGGCAATATAACATGAGGGACAAGAGTTTTGGTAGGAAGAGGCCTCGTGATTCCGAGGATATTGATCGGACGGATAGAGATAGAGATAGAGATCGGATTAGGAGAAGAGAGAACTATGGTACTTACAATAGGAGGGAGGTTGGTAGAGATAGGGAGGCAAAGGGTTATTGGGAGAGGGATAAGTTGGGAACGAATGAGCTTGTTTTTCGATTAGGGACTTACGAACCTGATCATAACAAAGAGTGTAGGATAGCTGATATGAAAAACCAGCAGTTCAATGAGAAGGCTGAGAAGAAACCGGAAGAGGTTAAAGAAAAAATTCCTGAGGAACAAGCTAGGAAGTATCAATTGGATGTGCTCGAACAAGCAAAGAAGAGAAATACAATAGCTTTTCTTGAAACAGGTGCCGGAAAGACACTCATTGCTGTGCTTCTCATGCAAAGTGTTTGCAGTGATATGCAGCAAAAGCAGAACAAAAAGATGCTCGCTGTATTTCTCGTTCCTAAAGTTCCCCTTGTTTATCAGCAAGCTGAAGTTATTCGTGAGCGAACTGGTTTCCAAGTGGGCCATTATTGTGGTGAGATGGGCCAAGATTTCTGGGACACCCGAAGATGGCAGCATGAATTCGACACGAAACAGGTTCTGGTAATGACAGCTCAAATTCTGTTGAACATTCTGAGGCACAGCATTATAAAAATGGAAGCAATCAATCTCTTGATTCTGGACGAGTGTCATCACGCTGTGAAGAAACATCCATATTCTTTGGTGATGTCTGAGTTCTATCATACAACCCCAAAGGAGAAGAGACCATCTGTATTTGGGATGACTGCTTCTCCTGTTAATTTAAAAGGTGTTTCCAGCCAAATAGATTGTGCAATAAAGATTCGTAATCTTGAAAGTAAACTAGATTCCATTGTTTGTACCATAAAGGACCGTAAGGAGCTTGAAAAGCATGTTCCGATGCCTTCCGAAACAGTGGTGCAGTATGATAAAGGAGCTAGCTTATGGTCCCTTCATGAGCAGCTAAAAAAGATGGAAATGGAAGTTGAAGAGGCTGCAAAGTTCAGCTCTAGAAAAAGTAAATGGCAGTTTATGGGAGCCAGAGATGCTGGGGCCAAGGAAGAGCTGCGCCAAGTGTATGGTGTTTCCGAAAGAACAGAAAGTGATGGGGCTGTTAACCTAATTCAAAAGTTGAGGGCTATTAATTATGCGCTCGGTGAACTGGGTCAGTGGTGTGCTTACAAGGTTGCACAATCTTTCTTGACAGCTTTACAAAATGACGAGAGGGCAAACTACCAGCTTGATGTCAAGTTTCAGGAATCCTACCTGAGCAAAGTTGTAAATCTCTTACAATGTCAGCTATCAGAGGGAGCTGTTTCAGACAAGGAAACAAAAGTAGCAGATTCAGAAAATGGCATTTCTTGTGATGGAAATGACCCTGATGAGATGGAGGAAGGAGAGCTACCTGATAGTCATGTTGTCTCTGGCGGGGAGCATGTAGATGTGATAATGGGAGCTGCTGTAGCTGATGGGAAGGTGACTCCAAAAGTGCAGTCACTGGTGAAAGTACTTCTCAAGTATCAGCATACAGAAGATTTTCGCGCAATCATCTTTGTCGAGCGAGTTGTGTCTGCTTTAGTTCTTCCCAAGGTCTTCGCAGAACTTCCTTCTTTAGGTTTTATAGAGTGTGCCAGCCTTATCGGCCACAACAATAGTCAGGAAATGAGAAGTTGCCAAATGCAGGACACTATTGCCAAATTCAAGGATGGCCGTGTGACACTGTTAGTTGCGACTAGTGTTGCCGAGGAAGGACTTGATATCCGGCAGTGCAATGTCGTTATTCGGTTTGACCTTGCAAAAACTGTTTTGGCTTACATTCAGTCCAGGGGTCGTGCTAGAAAACCCGGGTCTGATTACATCTTAATGGTGGAGAGAGGAAATCTGGCGCATGAAGCATTCTTAAGGAATGCCAGGAATAGTGAGGAGACTTTACGGAAGGAAGCAATAGAGAGAACTGACCTGAGTCATCTGAAGGATAGTGATAGGTTGATTTCAGTGGATACAACGCCAGGGACAGTGTACCAGGTGGAGTCAACTGGTGCTGTTGTGAGCTTAAACTCTGCTGTTGGACTCATCCATTTCTACTGCTCTCAGCTTCCCAGCGACAGGTACTCAATTCTTCGTCCTGAGTTTGTTATGGTGAGGCATGAGAAGCAAGGAGGTCACACTGAATATTCATGCAAACTTCAACTCCCTTGTAACGCACAATTTGAGACACTTGAGGGTCCCGTGTCCAGTTCTATGCACCTTGCACAGCAGGCTGTTTGCTTGGCTGCTTGCAAGAAACTCCATGAGATGGGAGCCTTTACCGACATGCTCTTGCCAGACAAGGGAAGCGGAGAAGAAAAAGAACAGGTTGACCAGAATGATGATGGAGATCCACTTCCTGGAACTGCTAGGCACAGGGAGTTCTATCCCGAAGGTGTAGCTAATATTCTCAAGGGAGAATGGATCTTATCCGGAAGAGATTTGGGCCGTGACTCGGAATTGATTCATGTTTACATGTATGGCGTGAAATGTGTAAACTCTGGCTCTTCAAAAGATCCTTTCTTGAATCAAGTTTCAGATTTCGCAGTTCTAGTAGGAAATGAACTGGATGCAGAGGTGTTATCGATGTCGATGGATCTATTTATCGCTCGAACCATGACTACAAAGGCATCCCTTGTCTTCAGGGGTTCAATATATATCACTGAAAGTCAGTTGGCGTCCCTTAAAAGTTTTCATGTAAGATTGATGAGTATTGTACTGGACGTGGATGTCAAACCTTCCACTACTCCTTGGGATCCTGCAAAGGCTTATTTATTTGTCCCTGTGGTTAGCGATAAGTGTGTGGATCCTATGAAAGAAATAGATTGGGATCTGGTTGAAAATATAAATGGTGCAGAGGCTTGGAATAATCCCCTTCAAAGAGCTCGGCCTGATGTTTCCCTTGGCACAAGTGAGCGTACGCTAGGTGGAGACAGAAGGGAGTATGGTTTCGCGAAATTGCGTCATGGCATGGCTTTTGGACAAAAATCCCATCCAACCTATGGTATCAGAGGAGCTGTGGCACAGTTTGATGTTGTTAAAGCTTCCGGATTGATGCCTGATCGGGATGCCCTAGAGATTAGAAAGCTGGTAGATTTGCCCAAAGACAAGTTATTTATGGCTGATCCTACATGTGTCAGTGTCGAAGGCTTGGTCGGGAGGATTGTGACAGCTGCTCACTCAGGGAAGAGGTTTTATGTGGATTCAATATGCTATGATATGACGGCAGAAAACTCTTTCCCCCGGAAAGAAGGCTACCTTGGCCCTGTGGAGTACAGCTCATATGCTGATTATTACAAGCAAAAGTATGGAGTTGAGTTGGTCTATAAGCAACAACCTTTAATAAGAGGACGCGGTGTCTCGTATTGCAAGAATCTCCTGTCTCCGCGATTTGAACATACGGAAGAACAAGATGGTGAATCTGAAGAAACTCTTGACAAAACGTACTATGTTTTCCTCCCTCCCGAGCTTTGTTTAGTACACCCATTGCCCGGATCACTTGCCCGTGGTGCCCAGAGACTACCCTCAATAATGAGAAGGGTTGAAAGCATGCTGCTTGCAGTCGAACTTAAAGATATGATAAACTATTCTGTCCCTGCTTCAAAGATCCTGGAAGCCTTGACAGCCGCTTCATGCCAGGAGACATTTTGCTACGAAAGAGCAGAACTTCTGGGTGATGCTTACCTGAAATGGGTTGTTAGTCGATTTCTCTTTCTTAAATACCCTCAGAAACACGAGGGTCAGCTTACTAGGATGAGGCAACAAATGGTTAGCAACATGGTTTTATACCAGTATGCATTGAAAAAAGGACTTCAGTCATATATCCAATCAGATCGCTTTTCTCCATCTCGGTGGGCTGCTCCTGGGGTGTTACCTGTCTTTGATGAGTATACAAACGATGGAGAATCATCCTTGTTTGACGAGGAGGAAAACCCTGTAGGGCAAATGGATCGTCCTGGTGATATATATGAGGATGATGAGCTGGAGGATGGGGAGCTTGAAGGTGACTCGAGTTCTTATAGAGTCCTTTCCAGCAAGACACTTGCAGATGTTGTCGAAGCACTTATCGGAGTTTATTATGTTGAAGGTGGGAAAAATGCTGCTAACCATCTCATGAAGTGGATTGGAATTGAGATAGAGCTTGACCCCAATGGGCTAGAGTGCATACCGAAGCCATCTAGCGTTCCAGAGAGTGTACTTAGGAGTGTCAACTTTGATACCTTAGAAGGTGCTCTGAATTTTAAATTTAGTGATAGGGGCTTTTTGGTAGAAGCCATTAGTCATGCTTCTCGACCATCTGCTGGAGTATCCTGCTATCAGCGGCTGGAGTTTGTTGGTGATGCCGTCTTAGATCGATCTCATTACCAGGCACTTGTTCTTCACGTACACAAATCTACCTCCAGGCAGGTTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1700

Amino Acids

190.23

Weight (kDa)

5.45

Isoelectric Point (pI)

40.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	312 - 478	6.3e-15	Type III restriction enzyme, res subunit
DEAD	PF00270	313 - 478	2.4e-16	DEAD/DEAH box helicase
Helicase_C	PF00271	715 - 832	8.6e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	907 - 995	3.3e-21	Dicer dimerisation domain
PAZ	PF02170	1278 - 1409	1.1e-17	PAZ domain
Ribonucleas_3_3	PF14622	1434 - 1504	4.9e-08	Ribonuclease-III-like
Ribonuclease_3	PF00636	1447 - 1592	2.1e-21	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01040 AT1G01040
fragaria_vesca	FvH4_7g17920 FvH4_7g17920 FvH4_7g17920 FvH4_7g17920
malus_domestica	MD01G1091600.v1.1 MD07G1161800.v1.1
prunus_persica	Prupe.2G200900_v2.0.a1
pyrus_communis	pycom01g11560 pycom07g15740
rosa_chinensis	RchiOBHm_Chr1g0361361
rosa_laevigata	RLG00000027722
rosa_multiflora	Rmu_sc0002119.1_g000026
rosa_roxburghii	Rroxscaffold_4G00295020
rosa_rugosa	Rorug01G0288900
rosa_samantha	Rh1AG299700 Rh1BG263500 Rh1CG281100 Rh1DG293500
rosa_wichuraiana	Rw1G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1289
AasI	GACNNNNNNGTC	1 cut(s)	3413
Acc36I	ACCTGC	1 cut(s)	5080
AccB1I	GGYRCC	2 cut(s)	1001, 4193
AccB7I	CCANNNNNTGG	5 cut(s)	146, 2953, 3044, 3686, 4985
AccBSI	CCGCTC	1 cut(s)	261
AccI	GTMKAC	1 cut(s)	4574
AccII	CGCG	4 cut(s)	2199, 3638, 4038, 4072
AccIII	TCCGGA	2 cut(s)	3116, 3731
AcoI	YGGCCR	3 cut(s)	171, 2299, 2365
AcsI	RAATTY	7 cut(s)	292, 915, 1220, 1257, 2354, 4921, 4928
AcuI	CTGAAG	5 cut(s)	1149, 2648, 3310, 4128, 4481
AcyI	GRCGYC	1 cut(s)	3362
AdeI	CACNNNGTG	1 cut(s)	1339
AflII	CTTAAG	1 cut(s)	2557
AflIII	ACRYGT	2 cut(s)	3159, 3815
AhdI	GACNNNNNGTC	1 cut(s)	4040
AhlI	ACTAGT	1 cut(s)	2390
AjiI	CACGTC	1 cut(s)	3409
AjuI	GAANNNNNNNTTGG	4 cut(s)	929, 961, 3927, 3959
AloI	GAACNNNNNNTCC	2 cut(s)	393, 425
Alw21I	GWGCWC	4 cut(s)	43, 957, 3571, 4918
Alw26I	GTCTC	9 cut(s)	1396, 2083, 2576, 2864, 3609, 4048, 4071, 4195, 4319
AlwNI	CAGNNNCTG	3 cut(s)	1240, 2155, 5008
Ama87I	CYCGRG	3 cut(s)	2501, 4148, 4693
Aor13HI	TCCGGA	2 cut(s)	3116, 3731
ApoI	RAATTY	7 cut(s)	292, 915, 1220, 1257, 2354, 4921, 4928
AseI	ATTAAT	1 cut(s)	1800
Asp700I	GAANNNNTTC	4 cut(s)	915, 1134, 2193, 3147
AspLEI	GCGC	4 cut(s)	1728, 1810, 2201, 2545
AsuC2I	CCSGG	4 cut(s)	2502, 2503, 3928, 4176
AsuNHI	GCTAGC	1 cut(s)	1595
AvaI	CYCGRG	3 cut(s)	2501, 4148, 4693
AvaII	GGWCC	6 cut(s)	149, 287, 401, 1528, 1605, 2882
AxyI	CCTNAGG	1 cut(s)	921
BaeGI	GKGCMC	1 cut(s)	4198
BalI	TGGCCA	1 cut(s)	173
BamHI	GGATCC	2 cut(s)	3439, 3490
BanI	GGYRCC	2 cut(s)	1001, 4193
BanII	GRGCYC	1 cut(s)	3571
BarI	GAAGNNNNNNTAC	2 cut(s)	2043, 2075
BauI	CACGAG	2 cut(s)	642, 4418
BbsI	GAAGAC	2 cut(s)	2242, 3316
Bbv12I	GWGCWC	4 cut(s)	43, 957, 3571, 4918
BceAI	ACGGC	4 cut(s)	2352, 3117, 3924, 5012
BcgI	CGANNNNNNTGC	2 cut(s)	4616, 4650
BciVI	GTATCC	2 cut(s)	2650, 5002
BclI	TGATCA	2 cut(s)	235, 826
BcnI	CCSGG	4 cut(s)	2502, 2503, 3928, 4176
BcoDI	GTCTC	9 cut(s)	1396, 2083, 2576, 2864, 3609, 4048, 4071, 4195, 4319
BcuI	ACTAGT	1 cut(s)	2390
BfmI	CTRYAG	2 cut(s)	2118, 4620
BfrI	CTTAAG	1 cut(s)	2557
BfuAI	ACCTGC	1 cut(s)	5080
BfuI	GTATCC	2 cut(s)	2650, 5002
BglI	GCCNNNNNGGC	1 cut(s)	2298
BmcAI	AGTACT	1 cut(s)	2166
Bme18I	GGWCC	6 cut(s)	149, 287, 401, 1528, 1605, 2882
BmeRI	GACNNNNNGTC	1 cut(s)	4040
BmeT110I	CYCGRG	3 cut(s)	2501, 4148, 4693
BmgBI	CACGTC	1 cut(s)	3409
BmrI	ACTGGG	3 cut(s)	39, 39, 1829
BmtI	GCTAGC	1 cut(s)	1599
BmuI	ACTGGG	3 cut(s)	39, 39, 1829
BoxI	GACNNNNGTC	3 cut(s)	1326, 2619, 4498
BpiI	GAAGAC	2 cut(s)	2242, 3316
BplI	GAGNNNNNCTC	4 cut(s)	527, 559, 3947, 3979
BpmI	CTGGAG	5 cut(s)	123, 4688, 5007, 5030, 5068
Bpu10I	CCTNAGC	1 cut(s)	1925
BpuEI	CTTGAG	3 cut(s)	2156, 2897, 3080
BpuMI	CCSGG	4 cut(s)	2502, 2503, 3928, 4176
Bsa29I	ATCGAT	2 cut(s)	3269, 5038
BsaAI	YACGTR	1 cut(s)	5068
BsaBI	GATNNNNATC	4 cut(s)	660, 687, 3033, 3513
BsaHI	GRCGYC	1 cut(s)	3362
BsaI	GGTCTC	1 cut(s)	1396
BsaWI	WCCGGW	3 cut(s)	895, 3116, 3731
BsaXI	ACNNNNNCTCC	8 cut(s)	191, 221, 1414, 1444, 2103, 2133, 2832, 2862
Bse118I	RCCGGY	1 cut(s)	361
Bse21I	CCTNAGG	1 cut(s)	921
Bse3DI	GCAATG	5 cut(s)	187, 324, 1017, 2434, 4169
Bse8I	GATNNNNATC	4 cut(s)	660, 687, 3033, 3513
BseAI	TCCGGA	2 cut(s)	3116, 3731
BseCI	ATCGAT	2 cut(s)	3269, 5038
BseJI	GATNNNNATC	4 cut(s)	660, 687, 3033, 3513
BseMI	GCAATG	5 cut(s)	187, 324, 1017, 2434, 4169
BseRI	GAGGAG	9 cut(s)	20, 65, 144, 212, 439, 505, 2594, 3711, 4622
BseSI	GKGCMC	1 cut(s)	4198
BseYI	CCCAGC	2 cut(s)	1706, 2756
BsgI	GTGCAG	3 cut(s)	1598, 2168, 3558
Bsh1236I	CGCG	4 cut(s)	2199, 3638, 4038, 4072
BshNI	GGYRCC	2 cut(s)	1001, 4193
BshVI	ATCGAT	2 cut(s)	3269, 5038
BsiHKAI	GWGCWC	4 cut(s)	43, 957, 3571, 4918
BsiHKCI	CYCGRG	3 cut(s)	2501, 4148, 4693
BsiSI	CCGG	9 cut(s)	362, 896, 1005, 2419, 2502, 3117, 3732, 3928, 4176
BsiWI	CGTACG	1 cut(s)	3602
BsmAI	GTCTC	9 cut(s)	1396, 2083, 2576, 2864, 3609, 4048, 4071, 4195, 4319
BsmI	GAATGC	5 cut(s)	310, 388, 580, 2552, 2569
Bso31I	GGTCTC	1 cut(s)	1396
BsoBI	CYCGRG	3 cut(s)	2501, 4148, 4693
Bsp1286I	GDGCHC	5 cut(s)	43, 957, 3571, 4198, 4918
Bsp13I	TCCGGA	2 cut(s)	3116, 3731
Bsp68I	TCGCGA	1 cut(s)	3638
BspDI	ATCGAT	2 cut(s)	3269, 5038
BspEI	TCCGGA	2 cut(s)	3116, 3731
BspFNI	CGCG	4 cut(s)	2199, 3638, 4038, 4072
BspHI	TCATGA	2 cut(s)	1612, 4797
BspMI	ACCTGC	1 cut(s)	5080
BspOI	GCTAGC	1 cut(s)	1599
BspQI	GCTCTTC	2 cut(s)	1713, 3196
BspT107I	GGYRCC	2 cut(s)	1001, 4193
BspTI	CTTAAG	1 cut(s)	2557
BspTNI	GGTCTC	1 cut(s)	1396
BsrBI	CCGCTC	1 cut(s)	261
BsrDI	GCAATG	5 cut(s)	187, 324, 1017, 2434, 4169
BsrFI	RCCGGY	1 cut(s)	361
BssAI	RCCGGY	1 cut(s)	361
BssNAI	GTATAC	1 cut(s)	4575
BssNI	GRCGYC	1 cut(s)	3362
BssSI	CACGAG	2 cut(s)	642, 4418
BssT1I	CCWWGG	5 cut(s)	1713, 2244, 3434, 3586, 3943
Bst1107I	GTATAC	1 cut(s)	4575
Bst2BI	CACGAG	2 cut(s)	642, 4418
Bst4CI	ACNGT	8 cut(s)	99, 251, 1532, 1573, 2379, 2461, 2674, 3711
BstACI	GRCGYC	1 cut(s)	3362
BstAFI	CTTAAG	1 cut(s)	2557
BstAPI	GCANNNNNTGC	1 cut(s)	186
BstBAI	YACGTR	1 cut(s)	5068
BstC8I	GCNNGC	9 cut(s)	268, 1083, 1125, 1597, 2290, 2916, 2935, 4235, 4242
BstDSI	CCRYGG	1 cut(s)	4189
BstENI	CCTNNNNNAGG	1 cut(s)	2268
BstFNI	CGCG	4 cut(s)	2199, 3638, 4038, 4072
BstHHI	GCGC	4 cut(s)	1728, 1810, 2201, 2545
BstMAI	GTCTC	9 cut(s)	1396, 2083, 2576, 2864, 3609, 4048, 4071, 4195, 4319
BstNSI	RCATGY	6 cut(s)	1553, 2096, 2974, 3163, 3819, 4237
BstPAI	GACNNNNGTC	3 cut(s)	1326, 2619, 4498
BstSFI	CTRYAG	2 cut(s)	2118, 4620
BstSLI	GKGCMC	1 cut(s)	4198
BstUI	CGCG	4 cut(s)	2199, 3638, 4038, 4072
BstV2I	GAAGAC	2 cut(s)	2242, 3316
BstX2I	RGATCY	8 cut(s)	3034, 3108, 3199, 3280, 3439, 3490, 3514, 4290
BstXI	CCANNNNNNTGG	3 cut(s)	1172, 1736, 4536
BstYI	RGATCY	8 cut(s)	3034, 3108, 3199, 3280, 3439, 3490, 3514, 4290
BstZ17I	GTATAC	1 cut(s)	4575
Bsu15I	ATCGAT	2 cut(s)	3269, 5038
Bsu36I	CCTNAGG	1 cut(s)	921
BsuI	GTATCC	2 cut(s)	2650, 5002
BsuTUI	ATCGAT	2 cut(s)	3269, 5038
BtgI	CCRYGG	1 cut(s)	4189
BtrI	CACGTC	1 cut(s)	3409
BtsI	GCAGTG	2 cut(s)	1054, 2429
BtuMI	TCGCGA	1 cut(s)	3638
BveI	ACCTGC	1 cut(s)	5080
Cac8I	GCNNGC	9 cut(s)	268, 1083, 1125, 1597, 2290, 2916, 2935, 4235, 4242
CaiI	CAGNNNCTG	3 cut(s)	1240, 2155, 5008
CciI	TCATGA	2 cut(s)	1612, 4797
CfoI	GCGC	4 cut(s)	1728, 1810, 2201, 2545
Cfr10I	RCCGGY	1 cut(s)	361
Cfr9I	CCCGGG	1 cut(s)	2501
ClaI	ATCGAT	2 cut(s)	3269, 5038
CseI	GACGC	3 cut(s)	3351, 3635, 4044
CsiI	ACCWGGT	1 cut(s)	2679
DraI	TTTAAA	2 cut(s)	1443, 4927
DraIII	CACNNNGTG	1 cut(s)	1339
DrdI	GACNNNNNNGTC	1 cut(s)	3413
DriI	GACNNNNNGTC	1 cut(s)	4040
DseDI	GACNNNNNNGTC	1 cut(s)	3413
EaeI	YGGCCR	3 cut(s)	171, 2299, 2365
Eam1105I	GACNNNNNGTC	1 cut(s)	4040
Ecl136II	GAGCTC	1 cut(s)	3569
Eco130I	CCWWGG	5 cut(s)	1713, 2244, 3434, 3586, 3943
Eco147I	AGGCCT	1 cut(s)	640
Eco24I	GRGCYC	1 cut(s)	3571
Eco31I	GGTCTC	1 cut(s)	1396
Eco32I	GATATC	1 cut(s)	2416
Eco47I	GGWCC	6 cut(s)	149, 287, 401, 1528, 1605, 2882
Eco53kI	GAGCTC	1 cut(s)	3569
Eco57I	CTGAAG	5 cut(s)	1149, 2648, 3310, 4128, 4481
Eco81I	CCTNAGG	1 cut(s)	921
Eco88I	CYCGRG	3 cut(s)	2501, 4148, 4693
EcoICRI	GAGCTC	1 cut(s)	3569
EcoNI	CCTNNNNNAGG	1 cut(s)	2268
EcoO109I	RGGNCCY	1 cut(s)	2882
EcoRI	GAATTC	2 cut(s)	292, 1220
EcoRV	GATATC	1 cut(s)	2416
EcoT14I	CCWWGG	5 cut(s)	1713, 2244, 3434, 3586, 3943
EcoT22I	ATGCAT	1 cut(s)	4483
EcoT38I	GRGCYC	1 cut(s)	3571
ErhI	CCWWGG	5 cut(s)	1713, 2244, 3434, 3586, 3943
FalI	AAGNNNNNCTT	6 cut(s)	1095, 1127, 3927, 3959, 4101, 4133
FauI	CCCGC	1 cut(s)	2078
FauNDI	CATATG	1 cut(s)	3967
FbaI	TGATCA	2 cut(s)	235, 826
FblI	GTMKAC	1 cut(s)	4574
FriOI	GRGCYC	1 cut(s)	3571
GlaI	GCGC	4 cut(s)	1727, 1809, 2200, 2544
GsaI	CCCAGC	2 cut(s)	1710, 2760
GsuI	CTGGAG	5 cut(s)	123, 4688, 5007, 5030, 5068
HapII	CCGG	9 cut(s)	362, 896, 1005, 2419, 2502, 3117, 3732, 3928, 4176
HgaI	GACGC	3 cut(s)	3351, 3635, 4044
HhaI	GCGC	4 cut(s)	1728, 1810, 2201, 2545
Hin1I	GRCGYC	1 cut(s)	3362
Hin6I	GCGC	4 cut(s)	1726, 1808, 2199, 2543
HinP1I	GCGC	4 cut(s)	1726, 1808, 2199, 2543
HincII	GTYRAC	5 cut(s)	1774, 2691, 3016, 4894, 5095
HindII	GTYRAC	5 cut(s)	1774, 2691, 3016, 4894, 5095
HindIII	AAGCTT	1 cut(s)	3726
HpaI	GTTAAC	1 cut(s)	1774
HpaII	CCGG	9 cut(s)	362, 896, 1005, 2419, 2502, 3117, 3732, 3928, 4176
HpyCH4III	ACNGT	8 cut(s)	99, 251, 1532, 1573, 2379, 2461, 2674, 3711
HpyCH4IV	ACGT	3 cut(s)	3408, 4127, 5067
HpySE526I	ACGT	3 cut(s)	3408, 4127, 5067
Hsp92I	GRCGYC	1 cut(s)	3362
HspAI	GCGC	4 cut(s)	1726, 1808, 2199, 2543
KflI	GGGWCCC	1 cut(s)	2882
Kpn2I	TCCGGA	2 cut(s)	3116, 3731
Ksp22I	TGATCA	2 cut(s)	235, 826
KspAI	GTTAAC	1 cut(s)	1774
LguI	GCTCTTC	2 cut(s)	1713, 3196
MabI	ACCWGGT	1 cut(s)	2679
MaeII	ACGT	3 cut(s)	3408, 4127, 5067
MaeIII	GTNAC	9 cut(s)	2134, 2151, 2371, 2819, 2855, 3134, 3850, 4554, 4688
MbiI	CCGCTC	1 cut(s)	261
MfeI	CAATTG	1 cut(s)	944
MflI	RGATCY	8 cut(s)	3034, 3108, 3199, 3280, 3439, 3490, 3514, 4290
MhlI	GDGCHC	5 cut(s)	43, 957, 3571, 4198, 4918
MlsI	TGGCCA	1 cut(s)	173
MluNI	TGGCCA	1 cut(s)	173
MlyI	GAGTC	8 cut(s)	386, 2131, 2629, 2696, 2721, 3131, 4685, 4716
MmeI	TCCRAC	3 cut(s)	379, 2704, 3704
Mox20I	TGGCCA	1 cut(s)	173
Mph1103I	ATGCAT	1 cut(s)	4483
MroI	TCCGGA	2 cut(s)	3116, 3731
MroXI	GAANNNNTTC	4 cut(s)	915, 1134, 2193, 3147
MscI	TGGCCA	1 cut(s)	173
MslI	CAYNNNNRTG	5 cut(s)	1022, 1170, 2097, 2519, 4534
Msp20I	TGGCCA	1 cut(s)	173
MspA1I	CMGCKG	2 cut(s)	3857, 5005
MspCI	CTTAAG	1 cut(s)	2557
MspI	CCGG	9 cut(s)	362, 896, 1005, 2419, 2502, 3117, 3732, 3928, 4176
MunI	CAATTG	1 cut(s)	944
Mva1269I	GAATGC	5 cut(s)	310, 388, 580, 2552, 2569
MvnI	CGCG	4 cut(s)	2199, 3638, 4038, 4072
NciI	CCSGG	4 cut(s)	2502, 2503, 3928, 4176
NdeI	CATATG	1 cut(s)	3967
NheI	GCTAGC	1 cut(s)	1595
NmeAIII	GCCGAG	2 cut(s)	2425, 3550
NmuCI	GTSAC	7 cut(s)	2134, 2151, 2371, 2819, 3134, 3850, 4688
NruI	TCGCGA	1 cut(s)	3638
NsiI	ATGCAT	1 cut(s)	4483
NspI	RCATGY	6 cut(s)	1553, 2096, 2974, 3163, 3819, 4237
PaeI	GCATGC	1 cut(s)	4237
PaeR7I	CTCGAG	1 cut(s)	4693
PagI	TCATGA	2 cut(s)	1612, 4797
PceI	AGGCCT	1 cut(s)	640
PciI	ACATGT	2 cut(s)	3159, 3815
PciSI	GCTCTTC	2 cut(s)	1713, 3196
PcsI	WCGNNNNNNNCGW	1 cut(s)	287
PctI	GAATGC	5 cut(s)	310, 388, 580, 2552, 2569
PdmI	GAANNNNTTC	4 cut(s)	915, 1134, 2193, 3147
Pfl23II	CGTACG	1 cut(s)	3602
PflMI	CCANNNNNTGG	5 cut(s)	146, 2953, 3044, 3686, 4985
PfoI	TCCNGGA	2 cut(s)	3043, 4293
PleI	GAGTC	8 cut(s)	386, 2131, 2628, 2695, 2721, 3131, 4685, 4715
PpsI	GAGTC	8 cut(s)	386, 2131, 2628, 2695, 2721, 3131, 4685, 4715
Ppu21I	YACGTR	1 cut(s)	5068
PpuMI	RGGWCCY	1 cut(s)	2882
PscI	ACATGT	2 cut(s)	3159, 3815
PshAI	GACNNNNGTC	3 cut(s)	1326, 2619, 4498
PshBI	ATTAAT	1 cut(s)	1800
PsiI	TTATAA	1 cut(s)	1289
Psp124BI	GAGCTC	1 cut(s)	3571
Psp5II	RGGWCCY	1 cut(s)	2882
PspFI	CCCAGC	2 cut(s)	1706, 2756
PspLI	CGTACG	1 cut(s)	3602
PspPPI	RGGWCCY	1 cut(s)	2882
PspXI	VCTCGAGB	1 cut(s)	4693
PsrI	GAACNNNNNNTAC	4 cut(s)	1080, 1112, 3289, 3321
PstNI	CAGNNNCTG	3 cut(s)	1240, 2155, 5008
PsuI	RGATCY	8 cut(s)	3034, 3108, 3199, 3280, 3439, 3490, 3514, 4290
PvuII	CAGCTG	1 cut(s)	3857
RruI	TCGCGA	1 cut(s)	3638
RseI	CAYNNNNRTG	5 cut(s)	1022, 1170, 2097, 2519, 4534
SacI	GAGCTC	1 cut(s)	3571
SapI	GCTCTTC	2 cut(s)	1713, 3196
ScaI	AGTACT	1 cut(s)	2166
SchI	GAGTC	8 cut(s)	386, 2131, 2629, 2696, 2721, 3131, 4685, 4716
SduI	GDGCHC	5 cut(s)	43, 957, 3571, 4198, 4918
SexAI	ACCWGGT	1 cut(s)	2679
SfcI	CTRYAG	2 cut(s)	2118, 4620
Sfr274I	CTCGAG	1 cut(s)	4693
SgrAI	CRCCGGYG	1 cut(s)	361
SinI	GGWCC	6 cut(s)	149, 287, 401, 1528, 1605, 2882
SlaI	CTCGAG	1 cut(s)	4693
SmaI	CCCGGG	1 cut(s)	2503
SmiMI	CAYNNNNRTG	5 cut(s)	1022, 1170, 2097, 2519, 4534
SmlI	CTYRAG	5 cut(s)	2171, 2557, 2876, 3095, 4693
SmoI	CTYRAG	5 cut(s)	2171, 2557, 2876, 3095, 4693
SpeI	ACTAGT	1 cut(s)	2390
SphI	GCATGC	1 cut(s)	4237
SseBI	AGGCCT	1 cut(s)	640
SspI	AATATT	2 cut(s)	2831, 3091
SstI	GAGCTC	1 cut(s)	3571
StuI	AGGCCT	1 cut(s)	640
StyI	CCWWGG	5 cut(s)	1713, 2244, 3434, 3586, 3943
TaaI	ACNGT	8 cut(s)	99, 251, 1532, 1573, 2379, 2461, 2674, 3711
TaiI	ACGT	3 cut(s)	3411, 4130, 5070
TatI	WGTACW	5 cut(s)	2164, 3400, 3957, 4162, 4879
TauI	GCSGC	4 cut(s)	167, 361, 4313, 5008
TseFI	GTSAC	7 cut(s)	2134, 2151, 2371, 2819, 3134, 3850, 4688
Tsp45I	GTSAC	7 cut(s)	2134, 2151, 2371, 2819, 3134, 3850, 4688
TspGWI	ACGGA	4 cut(s)	582, 683, 2605, 4100
TspMI	CCCGGG	1 cut(s)	2501
Van91I	CCANNNNNTGG	5 cut(s)	146, 2953, 3044, 3686, 4985
Vha464I	CTTAAG	1 cut(s)	2557
VpaK11BI	GGWCC	6 cut(s)	149, 287, 401, 1528, 1605, 2882
VspI	ATTAAT	1 cut(s)	1800
XagI	CCTNNNNNAGG	1 cut(s)	2268
XapI	RAATTY	7 cut(s)	292, 915, 1220, 1257, 2354, 4921, 4928
XbaI	TCTAGA	1 cut(s)	1667
XceI	RCATGY	6 cut(s)	1553, 2096, 2974, 3163, 3819, 4237
XcmI	CCANNNNNNNNNTGG	3 cut(s)	200, 3026, 3683
XhoI	CTCGAG	1 cut(s)	4693
XmaI	CCCGGG	1 cut(s)	2501
XmiI	GTMKAC	1 cut(s)	4574
XmnI	GAANNNNTTC	4 cut(s)	915, 1134, 2193, 3147
ZrmI	AGTACT	1 cut(s)	2166
Zsp2I	ATGCAT	1 cut(s)	4483

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.