pycom01g18270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004912
Physical Location & Seq
Reverse (-)
17309702 .. 17311438
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1611 bp
ATGGTTGATATGGAGAGAACAGAACTGAAGGAGTCAGATTCAATAGAACTGGACAATGGTATCACCCCAGATCGTTCTAATTCTATGTGGCTGGTAGAAAATCTTGACGAGATGGGCCAAAGGATTAAGCAAATGTTGAAGCTGATCAAAGAAAATGGCGATTCTTTGCCAATAAATGACGAAACATTTTCTCAGAAGAAGCCTGAATTGGTTGAGGAATTTCACAGAATGCATCGATCGCTGGCTGGATGCTATGAACACTTGACCAAAGAAGCGCATAAGGGGTTTGGGGATTCTGAATATGGTTTCGACCAACGCTCTCCTCTGCTGACTCCGGATGCTAAACCTGGTCTGCAGAAATCCGGCCATCAAGTTGTTGGGTTAGATATATCACCAAGCTCAGATGGTTCTAGCCCAGCTCTTTCTTTAAAGAATGGCAGTGAATCATCTTCCTCATCATCATCGGGATCCGAGTCAGAATCTCTTAACTCCTTCGTCAGCAATTACTTGGTTCCACCTCCGAACGATGATTTTAACAGCCAGGGATGGCAGCAGAAGATTACTGAGCTTGAAACCGAACTTTCTAGCTTGAGAGAGAAGCTCCAAATGAGGGAGGCTGATCTTAAAATGGACAAAATGCGGGTGTTTGAGCTGCAAAATCAGATAGTTGAGTTGGAGAGTCTGGCATCAGACAGAGAAAATGAGATTGGGAGATTGGTGGGAGACTTGGAAGTGACTAAGGAAAGGCTTAAGGGGTTGGACGAAGAGATTGTAAAGTTGAAGGATGAACATGCCCATAGAATTTCCGAAGGCGCTCATCAAATGCAAGGTCTGGAAGCCCAACTTGACTTGGAGAGAAAGCATGTTTCGGAGCTGGAGGAGAGGATAGTGAGGTACAATGCTGATATACACAGCCGTGATCTTGAGGTGATGCAGCTGAAGAGTGCATTGCATGATGCACAGGAACAATTCTCTCTTGAGAAAGCAAACCTGCAGGCTGACATTTCAAGTTTTTCAGAGAAACAAATCCTTTTGGATACAAGAGTCGAAGAATTGAGCTTCAGAAGCAAGAGTTTAGAAGACGAAATAAGGCAATGTGAAACGGATAAGATGGAAATGGAAAGGCTGCACGTTGTCCGCGAGATGGCATTGCAAGATGAAATAAGCCGCTTGAAAGTAGAAGTTGCGGAAAGAAATGGACATGTGGAAGCTGTAAATAAAGACTTCGACAGGTTTAAACTGAAATATGATATGCTGATGGCGGAGAAAGATGAGCTCAATGCTAGGACTCAAACTCTTATGGCGAATGTGAGTTCCCGCGACAATCAGATTCAGGAAATGGCGGGACATCTTAGTCTGTTACGAACAGAACACGAGGGTGTGATTGTTGGATCCGAATGTGCACATAGACTAGTAGATGAGTTGAAATCGAGAGTGGAGGAGTTGCAGCAAGAGGTGAAAAGGCAGAGTGTTGTGATCTCGGATGGGGCTGAGGAGAAAAGAGAGGTGATCCGGCAGCTTTGCTTCTCGCTGGAGCACTACAGGAGCGGATACCAAGAGCTTCGTCAAGCATTTACCGGGCACAGGCGGCGGGCGGTTGCAGCTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 KIP1

Protein Analysis

537

Amino Acids

61.28

Weight (kDa)

5.03

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 25 - 85 6.6e-15 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1353
Acc36I ACCTGC 1 cut(s) 999
AccBSI CCGCTC 1 cut(s) 1548
AccII CGCG 2 cut(s) 1140, 1320
AccIII TCCGGA 1 cut(s) 334
AclWI GGATC 5 cut(s) 462, 475, 1386, 1399, 1504
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 2 cut(s) 218, 801
AcuI CTGAAG 3 cut(s) 47, 959, 1045
AfaI GTAC 1 cut(s) 896
AfiI CCNNNNNNNGG 3 cut(s) 610, 1144, 1584
AflII CTTAAG 1 cut(s) 749
AflIII ACRYGT 1 cut(s) 1201
AgsI TTSAA 7 cut(s) 42, 139, 572, 781, 1008, 1174, 1426
AhlI ACTAGT 1 cut(s) 1411
AjnI CCWGG 2 cut(s) 346, 540
AjuI GAANNNNNNNTTGG 4 cut(s) 191, 223, 690, 722
AleI CACNNNNGTG 2 cut(s) 915, 1377
Alw21I GWGCWC 3 cut(s) 1278, 1405, 1539
Alw26I GTCTC 1 cut(s) 717
Alw44I GTGCAC 1 cut(s) 1401
AlwI GGATC 5 cut(s) 462, 475, 1386, 1399, 1504
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 2 cut(s) 115, 364
ApaLI GTGCAC 1 cut(s) 1401
ApeKI GCWGC 8 cut(s) 550, 652, 934, 1126, 1447, 1516, 1601, 1604
ApoI RAATTY 2 cut(s) 218, 801
ArsI GACNNNNNNTTYG 2 cut(s) 814, 846
AspLEI GCGC 2 cut(s) 277, 815
AspS9I GGNCC 1 cut(s) 115
AsuC2I CCSGG 1 cut(s) 1579
AsuHPI GGTGA 5 cut(s) 55, 384, 940, 1468, 1519
BaeGI GKGCMC 2 cut(s) 1405, 1584
BaeI ACNNNNGTAYC 4 cut(s) 43, 76, 1029, 1062
BamHI GGATCC 2 cut(s) 467, 1391
BanII GRGCYC 1 cut(s) 1278
BauI CACGAG 1 cut(s) 1373
BbsI GAAGAC 1 cut(s) 1086
Bbv12I GWGCWC 3 cut(s) 1278, 1405, 1539
BbvCI CCTCAGC 1 cut(s) 1491
BbvI GCAGC 7 cut(s) 562, 639, 946, 1113, 1459, 1528, 1591
BccI CCATC 8 cut(s) 106, 375, 398, 540, 1105, 1138, 1252, 1478
BceAI ACGGC 1 cut(s) 900
BciT130I CCWGG 2 cut(s) 348, 542
BciVI GTATCC 2 cut(s) 1030, 1544
BclI TGATCA 1 cut(s) 144
BcnI CCSGG 1 cut(s) 1579
BcoDI GTCTC 1 cut(s) 717
BcuI ACTAGT 1 cut(s) 1411
BfaI CTAG 4 cut(s) 411, 585, 1284, 1412
BfmI CTRYAG 3 cut(s) 353, 992, 1540
BfoI RGCGCY 1 cut(s) 816
BfrI CTTAAG 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 999
BfuI GTATCC 2 cut(s) 1030, 1544
Bme1390I CCNGG 3 cut(s) 348, 542, 1579
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 3 cut(s) 469, 513, 1393
BmrFI CCNGG 3 cut(s) 348, 542, 1579
BmsI GCATC 6 cut(s) 239, 241, 328, 695, 921, 946
BpiI GAAGAC 1 cut(s) 1086
BplI GAGNNNNNCTC 2 cut(s) 585, 617
BpmI CTGGAG 2 cut(s) 896, 1553
Bpu10I CCTNAGC 1 cut(s) 1491
BpuEI CTTGAG 3 cut(s) 610, 944, 998
BpuMI CCSGG 1 cut(s) 1579
Bsa29I ATCGAT 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 541
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 3 cut(s) 610, 1144, 1584
Bse1I ACTGG 1 cut(s) 54
Bse3DI GCAATG 3 cut(s) 947, 1100, 1148
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 2 cut(s) 348, 542
BseCI ATCGAT 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 541
BseGI GGATG 5 cut(s) 254, 343, 551, 790, 1489
BseLI CCNNNNNNNGG 3 cut(s) 610, 1144, 1584
BseMI GCAATG 3 cut(s) 947, 1100, 1148
BseMII CTCAG 4 cut(s) 206, 414, 555, 1482
BseNI ACTGG 1 cut(s) 54
BseRI GAGGAG 4 cut(s) 312, 893, 1454, 1508
BseSI GKGCMC 2 cut(s) 1405, 1584
BseXI GCAGC 7 cut(s) 562, 639, 946, 1113, 1459, 1528, 1591
BseYI CCCAGC 1 cut(s) 415
BsgI GTGCAG 1 cut(s) 1112
Bsh1236I CGCG 2 cut(s) 1140, 1320
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 2 cut(s) 117, 366
BshVI ATCGAT 1 cut(s) 235
BsiEI CGRYCG 1 cut(s) 239
BsiHKAI GWGCWC 3 cut(s) 1278, 1405, 1539
BsiSI CCGG 4 cut(s) 335, 363, 1513, 1578
BslFI GGGAC 1 cut(s) 1359
BslI CCNNNNNNNGG 3 cut(s) 610, 1144, 1584
BsmAI GTCTC 1 cut(s) 717
BsmFI GGGAC 1 cut(s) 1359
BsmI GAATGC 1 cut(s) 234
BsnI GGCC 2 cut(s) 117, 366
Bsp1286I GDGCHC 4 cut(s) 1278, 1405, 1539, 1584
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 9 cut(s) 70, 144, 236, 467, 619, 919, 1391, 1476, 1509
BspANI GGCC 2 cut(s) 117, 366
BspCNI CTCAG 4 cut(s) 205, 413, 556, 1483
BspDI ATCGAT 1 cut(s) 235
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 2 cut(s) 1140, 1320
BspLI GGNNCC 3 cut(s) 469, 513, 1393
BspMAI CTGCAG 2 cut(s) 357, 996
BspMI ACCTGC 1 cut(s) 999
BspPI GGATC 5 cut(s) 462, 475, 1386, 1399, 1504
BspTI CTTAAG 1 cut(s) 749
BsrBI CCGCTC 1 cut(s) 1548
BsrDI GCAATG 3 cut(s) 947, 1100, 1148
BsrI ACTGG 1 cut(s) 54
BssECI CCNNGG 1 cut(s) 541
BssMI GATC 9 cut(s) 70, 144, 236, 467, 619, 919, 1391, 1476, 1509
BssSI CACGAG 1 cut(s) 1373
Bst2BI CACGAG 1 cut(s) 1373
Bst2UI CCWGG 2 cut(s) 348, 542
Bst6I CTCTTC 2 cut(s) 759, 935
BstAFI CTTAAG 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 243, 996, 1593
BstDEI CTNAG 6 cut(s) 192, 400, 564, 738, 1352, 1491
BstF5I GGATG 5 cut(s) 254, 343, 551, 790, 1489
BstFNI CGCG 2 cut(s) 1140, 1320
BstH2I RGCGCY 1 cut(s) 816
BstHHI GCGC 2 cut(s) 277, 815
BstKTI GATC 9 cut(s) 73, 147, 239, 470, 622, 922, 1394, 1479, 1512
BstMAI GTCTC 1 cut(s) 717
BstMBI GATC 9 cut(s) 70, 144, 236, 467, 619, 919, 1391, 1476, 1509
BstMCI CGRYCG 1 cut(s) 239
BstMWI GCNNNNNNNGC 4 cut(s) 238, 1065, 1588, 1601
BstNI CCWGG 2 cut(s) 348, 542
BstNSI RCATGY 3 cut(s) 794, 866, 1205
BstSCI CCNGG 3 cut(s) 346, 540, 1577
BstSFI CTRYAG 3 cut(s) 353, 992, 1540
BstSLI GKGCMC 2 cut(s) 1405, 1584
BstUI CGCG 2 cut(s) 1140, 1320
BstV1I GCAGC 7 cut(s) 562, 639, 946, 1113, 1459, 1528, 1591
BstV2I GAAGAC 1 cut(s) 1086
BstX2I RGATCY 2 cut(s) 467, 1391
BstYI RGATCY 2 cut(s) 467, 1391
Bsu15I ATCGAT 1 cut(s) 235
BsuI GTATCC 2 cut(s) 1030, 1544
BsuRI GGCC 2 cut(s) 117, 366
BsuTUI ATCGAT 1 cut(s) 235
BtsCI GGATG 5 cut(s) 254, 343, 551, 790, 1489
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 1 cut(s) 445
BveI ACCTGC 1 cut(s) 999
Cac8I GCNNGC 3 cut(s) 243, 996, 1593
CfoI GCGC 2 cut(s) 277, 815
Cfr13I GGNCC 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 235
CsiI ACCWGGT 1 cut(s) 346
Csp6I GTAC 1 cut(s) 895
CviAII CATG 4 cut(s) 791, 863, 953, 1202
CviQI GTAC 1 cut(s) 895
DdeI CTNAG 6 cut(s) 192, 400, 564, 738, 1352, 1491
DpnI GATC 9 cut(s) 72, 146, 238, 469, 621, 921, 1393, 1478, 1511
DpnII GATC 9 cut(s) 70, 144, 236, 467, 619, 919, 1391, 1476, 1509
DraI TTTAAA 2 cut(s) 429, 1237
DrdI GACNNNNNNGTC 1 cut(s) 1353
DseDI GACNNNNNNGTC 1 cut(s) 1353
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 759, 935
EarI CTCTTC 2 cut(s) 759, 935
EciI GGCGGA 1 cut(s) 1277
Ecl136II GAGCTC 1 cut(s) 1276
Eco24I GRGCYC 1 cut(s) 1278
Eco53kI GAGCTC 1 cut(s) 1276
Eco57I CTGAAG 3 cut(s) 47, 959, 1045
EcoICRI GAGCTC 1 cut(s) 1276
EcoRII CCWGG 2 cut(s) 346, 540
EcoT22I ATGCAT 1 cut(s) 234
EcoT38I GRGCYC 1 cut(s) 1278
FaeI CATG 4 cut(s) 794, 866, 956, 1205
FalI AAGNNNNNCTT 2 cut(s) 828, 860
FaqI GGGAC 1 cut(s) 1359
FatI CATG 4 cut(s) 790, 862, 952, 1201
FauI CCCGC 4 cut(s) 633, 1325, 1336, 1584
FbaI TGATCA 1 cut(s) 144
FokI GGATG 5 cut(s) 261, 350, 558, 797, 1496
FriOI GRGCYC 1 cut(s) 1278
FspBI CTAG 4 cut(s) 411, 585, 1284, 1412
GlaI GCGC 2 cut(s) 276, 814
GsaI CCCAGC 1 cut(s) 419
GsuI CTGGAG 2 cut(s) 896, 1553
HaeII RGCGCY 1 cut(s) 816
HaeIII GGCC 2 cut(s) 117, 366
HapII CCGG 4 cut(s) 335, 363, 1513, 1578
HhaI GCGC 2 cut(s) 277, 815
Hin1II CATG 4 cut(s) 794, 866, 956, 1205
Hin6I GCGC 2 cut(s) 275, 813
HinP1I GCGC 2 cut(s) 275, 813
HpaII CCGG 4 cut(s) 335, 363, 1513, 1578
HphI GGTGA 5 cut(s) 55, 384, 940, 1468, 1519
Hpy166II GTNNAC 1 cut(s) 1403
Hpy188III TCNNGA 8 cut(s) 104, 335, 465, 833, 923, 977, 1334, 1431
Hpy8I GTNNAC 1 cut(s) 1403
HpyAV CCTTC 4 cut(s) 22, 502, 775, 803
HpyCH4IV ACGT 1 cut(s) 1131
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 1065, 1588, 1601
HpyF3I CTNAG 6 cut(s) 192, 400, 564, 738, 1352, 1491
HpySE526I ACGT 1 cut(s) 1131
Hsp92II CATG 4 cut(s) 794, 866, 956, 1205
HspAI GCGC 2 cut(s) 275, 813
Kpn2I TCCGGA 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 9 cut(s) 70, 144, 236, 467, 619, 919, 1391, 1476, 1509
LmnI GCTCC 4 cut(s) 606, 871, 1534, 1545
Lsp1109I GCAGC 7 cut(s) 562, 639, 946, 1113, 1459, 1528, 1591
LweI GCATC 6 cut(s) 239, 241, 328, 695, 921, 946
MabI ACCWGGT 1 cut(s) 346
MaeI CTAG 4 cut(s) 411, 585, 1284, 1412
MaeII ACGT 1 cut(s) 1131
MaeIII GTNAC 2 cut(s) 733, 1359
MalI GATC 9 cut(s) 72, 146, 238, 469, 621, 921, 1393, 1478, 1511
MbiI CCGCTC 1 cut(s) 1548
MboI GATC 9 cut(s) 70, 144, 236, 467, 619, 919, 1391, 1476, 1509
MboII GAAGA 7 cut(s) 208, 441, 568, 776, 952, 1061, 1091
MflI RGATCY 2 cut(s) 467, 1391
MhlI GDGCHC 4 cut(s) 1278, 1405, 1539, 1584
MluCI AATT 7 cut(s) 79, 206, 218, 502, 801, 968, 1052
MlyI GAGTC 6 cut(s) 41, 325, 482, 688, 1053, 1282
MmeI TCCRAC 3 cut(s) 654, 738, 1369
Mph1103I ATGCAT 1 cut(s) 234
MroI TCCGGA 1 cut(s) 334
MseI TTAA 7 cut(s) 126, 428, 486, 534, 624, 750, 1236
MslI CAYNNNNRTG 2 cut(s) 915, 1377
MspA1I CMGCKG 2 cut(s) 937, 1604
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 4 cut(s) 335, 363, 1513, 1578
MspR9I CCNGG 3 cut(s) 348, 542, 1579
MssI GTTTAAAC 1 cut(s) 1237
Mva1269I GAATGC 1 cut(s) 234
MvaI CCWGG 2 cut(s) 348, 542
MvnI CGCG 2 cut(s) 1140, 1320
MwoI GCNNNNNNNGC 4 cut(s) 238, 1065, 1588, 1601
NciI CCSGG 1 cut(s) 1579
NdeII GATC 9 cut(s) 70, 144, 236, 467, 619, 919, 1391, 1476, 1509
NlaIII CATG 4 cut(s) 794, 866, 956, 1205
NlaIV GGNNCC 3 cut(s) 469, 513, 1393
NmuCI GTSAC 1 cut(s) 733
NsiI ATGCAT 1 cut(s) 234
NspI RCATGY 3 cut(s) 794, 866, 1205
OliI CACNNNNGTG 2 cut(s) 915, 1377
PciI ACATGT 1 cut(s) 1201
PcsI WCGNNNNNNNCGW 1 cut(s) 1137
PctI GAATGC 1 cut(s) 234
PfeI GAWTC 6 cut(s) 38, 161, 293, 443, 479, 1330
Ple19I CGATCG 1 cut(s) 239
PleI GAGTC 6 cut(s) 40, 325, 481, 687, 1052, 1282
PmeI GTTTAAAC 1 cut(s) 1237
PpsI GAGTC 6 cut(s) 40, 325, 481, 687, 1052, 1282
PscI ACATGT 1 cut(s) 1201
Psp124BI GAGCTC 1 cut(s) 1278
Psp6I CCWGG 2 cut(s) 346, 540
PspFI CCCAGC 1 cut(s) 415
PspGI CCWGG 2 cut(s) 346, 540
PspN4I GGNNCC 3 cut(s) 469, 513, 1393
PspPI GGNCC 1 cut(s) 115
PstI CTGCAG 2 cut(s) 357, 996
PsuI RGATCY 2 cut(s) 467, 1391
PvuI CGATCG 1 cut(s) 239
PvuII CAGCTG 2 cut(s) 937, 1604
RsaI GTAC 1 cut(s) 896
RsaNI GTAC 1 cut(s) 895
RseI CAYNNNNRTG 2 cut(s) 915, 1377
SacI GAGCTC 1 cut(s) 1278
SaqAI TTAA 7 cut(s) 126, 428, 486, 534, 624, 750, 1236
Sau3AI GATC 9 cut(s) 70, 144, 236, 467, 619, 919, 1391, 1476, 1509
Sau96I GGNCC 1 cut(s) 115
SbfI CCTGCAGG 1 cut(s) 996
SchI GAGTC 6 cut(s) 41, 325, 482, 688, 1053, 1282
ScrFI CCNGG 3 cut(s) 348, 542, 1579
SdaI CCTGCAGG 1 cut(s) 996
SduI GDGCHC 4 cut(s) 1278, 1405, 1539, 1584
SexAI ACCWGGT 1 cut(s) 346
SfaNI GCATC 6 cut(s) 239, 241, 328, 695, 921, 946
SfcI CTRYAG 3 cut(s) 353, 992, 1540
SmiMI CAYNNNNRTG 2 cut(s) 915, 1377
SmlI CTYRAG 4 cut(s) 589, 749, 923, 977
SmoI CTYRAG 4 cut(s) 589, 749, 923, 977
SpeI ACTAGT 1 cut(s) 1411
Sse8387I CCTGCAGG 1 cut(s) 996
Sse9I AATT 7 cut(s) 79, 206, 218, 502, 801, 968, 1052
SspMI CTAG 4 cut(s) 411, 585, 1284, 1412
SstI GAGCTC 1 cut(s) 1278
StyD4I CCNGG 3 cut(s) 346, 540, 1577
TaiI ACGT 1 cut(s) 1134
TaqI TCGA 5 cut(s) 235, 309, 1047, 1227, 1430
TasI AATT 7 cut(s) 79, 206, 218, 502, 801, 968, 1052
TauI GCSGC 2 cut(s) 1170, 1591
TfiI GAWTC 6 cut(s) 38, 161, 293, 443, 479, 1330
Tru1I TTAA 7 cut(s) 126, 428, 486, 534, 624, 750, 1236
Tru9I TTAA 7 cut(s) 126, 428, 486, 534, 624, 750, 1236
TscAI CASTG 1 cut(s) 445
TseFI GTSAC 1 cut(s) 733
TseI GCWGC 8 cut(s) 550, 652, 934, 1126, 1447, 1516, 1601, 1604
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 3 cut(s) 270, 801, 1173
TspGWI ACGGA 1 cut(s) 1118
TspRI CASTG 1 cut(s) 445
Vha464I CTTAAG 1 cut(s) 749
VneI GTGCAC 1 cut(s) 1401
XapI RAATTY 2 cut(s) 218, 801
XceI RCATGY 3 cut(s) 794, 866, 1205
XcmI CCANNNNNNNNNTGG 1 cut(s) 374
XspI CTAG 4 cut(s) 411, 585, 1284, 1412
Zsp2I ATGCAT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.