pycom01g22160

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007019
Physical Location & Seq
Forward (+)
19877584 .. 19879573
1990 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 795 bp
ATGGCGGAGCCAAAACCAACGCCCACGGCTGAATCGCTGATGGACAAGATCTACAACCACCACTCTTCGTCGTCGTCGGACTCCGACGACGACAAGAAGTCCAAGCACGCTGCTTCCGTCAAAGCCAAGGTGGAAGACGAAAAGCCGTCGGCGCGCGATTCCGTCAAGTCCAAGATTTTCAGGCTCTTCGGCAGGGAAAAGCCCGTTCACAAGGTCTTGGGCGGCGGCAAACCTGCTGATGTCTTCCTATGGAGGAACAAGAAAATATCTGCAAGTGTGCTTGCCGGTGCCACTGTTTTATGGGTTTTCTTTGAGTTGCTCGAATACCATCTAATCACGCTGGTGTGCCATTTGTTGATACTTTTTCTGGCTGTCTTTTACCTGTGGTCCAATGCATCCAACTTTATCAACAAGTCGCAACCACAAATCCCAAAGGTTCAAATTCCAGAGAAGACTGTCCTTGAGCTTGCCTCTGCTCTGAGAATTGAACTCAACAAAGGATTTTATGTATTGCGGGATATTGCATCTGGGAGAGATTTGAAGGCATTCCTCGGTGTTATAGCTGTCTTGTGGATTCTATCGGTGGTTGGGAAATGGTTCAACTTTCTGACCTTATTCTACATACTTTTTGTGTTGCTGCACACACTGCCCGTGATTTATGAGAAATACGATGACCAGATTGATGCCTTTGGCGAGAAGGCGTGGATCGAAATAAAGAAGCAGTACGCAGTGTTTGATGCCAAGGTTTTGAGTAAAATCCCCAAGGGTCCTGTGAAGGAGAAGAAGAAGGATTAG

Protein Analysis

265

Amino Acids

29.94

Weight (kDa)

9.42

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 79 - 233 1.9e-58 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 241
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 2 cut(s) 154, 156
AciI CCGC 4 cut(s) 5, 222, 225, 514
AclWI GGATC 1 cut(s) 713
AcsI RAATTY 1 cut(s) 441
AfaI GTAC 1 cut(s) 725
AgsI TTSAA 4 cut(s) 440, 488, 541, 601
AhdI GACNNNNNGTC 1 cut(s) 97
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 2 cut(s) 466, 563
AluI AGCT 2 cut(s) 466, 563
AlwI GGATC 1 cut(s) 713
ApeKI GCWGC 2 cut(s) 110, 637
ApoI RAATTY 1 cut(s) 441
Asp700I GAANNNNTTC 2 cut(s) 545, 596
AspLEI GCGC 2 cut(s) 154, 156
AspS9I GGNCC 2 cut(s) 387, 767
AvaII GGWCC 2 cut(s) 387, 767
BanI GGYRCC 1 cut(s) 287
BbsI GAAGAC 3 cut(s) 141, 235, 458
BbvI GCAGC 2 cut(s) 97, 624
BccI CCATC 2 cut(s) 34, 336
BceAI ACGGC 2 cut(s) 42, 130
BfuAI ACCTGC 1 cut(s) 241
BglII AGATCT 1 cut(s) 48
BisI GCNGC 4 cut(s) 111, 223, 226, 638
BlsI GCNGC 4 cut(s) 112, 224, 227, 639
Bme18I GGWCC 2 cut(s) 387, 767
BmeRI GACNNNNNGTC 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 387, 767
BmiI GGNNCC 3 cut(s) 9, 289, 768
BmsI GCATC 4 cut(s) 404, 533, 673, 727
BpiI GAAGAC 3 cut(s) 141, 235, 458
BplI GAGNNNNNCTC 2 cut(s) 455, 487
BpuEI CTTGAG 1 cut(s) 482
BsaJI CCNNGG 5 cut(s) 24, 126, 550, 741, 762
Bse118I RCCGGY 1 cut(s) 284
BseDI CCNNGG 5 cut(s) 24, 126, 550, 741, 762
BseGI GGATG 1 cut(s) 395
BseMII CTCAG 1 cut(s) 470
BsePI GCGCGC 1 cut(s) 152
BseXI GCAGC 2 cut(s) 97, 624
BsgI GTGCAG 1 cut(s) 623
Bsh1236I CGCG 2 cut(s) 154, 156
BshNI GGYRCC 1 cut(s) 287
BsiSI CCGG 1 cut(s) 285
BsmI GAATGC 1 cut(s) 545
Bsp143I GATC 2 cut(s) 48, 705
BspACI CCGC 4 cut(s) 5, 222, 225, 514
BspCNI CTCAG 1 cut(s) 471
BspFNI CGCG 2 cut(s) 154, 156
BspLI GGNNCC 3 cut(s) 9, 289, 768
BspMI ACCTGC 1 cut(s) 241
BspPI GGATC 1 cut(s) 713
BspQI GCTCTTC 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 5 cut(s) 24, 126, 550, 741, 762
BssHII GCGCGC 1 cut(s) 152
BssMI GATC 2 cut(s) 48, 705
BssT1I CCWWGG 3 cut(s) 126, 741, 762
Bst4CI ACNGT 2 cut(s) 295, 457
Bst6I CTCTTC 2 cut(s) 70, 191
BstAPI GCANNNNNTGC 1 cut(s) 646
BstC8I GCNNGC 4 cut(s) 108, 154, 282, 468
BstDEI CTNAG 1 cut(s) 479
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 395
BstFNI CGCG 2 cut(s) 154, 156
BstHHI GCGC 2 cut(s) 154, 156
BstKTI GATC 2 cut(s) 51, 708
BstMBI GATC 2 cut(s) 48, 705
BstMWI GCNNNNNNNGC 2 cut(s) 151, 646
BstUI CGCG 2 cut(s) 154, 156
BstV1I GCAGC 2 cut(s) 97, 624
BstV2I GAAGAC 3 cut(s) 141, 235, 458
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 1 cut(s) 395
BtsI GCAGTG 2 cut(s) 644, 735
BtsIMutI CAGTG 3 cut(s) 291, 644, 735
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 4 cut(s) 108, 154, 282, 468
CfoI GCGC 2 cut(s) 154, 156
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 387, 767
Csp6I GTAC 1 cut(s) 724
CviJI RGCY 9 cut(s) 10, 29, 125, 145, 184, 202, 371, 466, 563
CviKI_1 RGCY 9 cut(s) 10, 29, 125, 145, 184, 202, 371, 466, 563
CviQI GTAC 1 cut(s) 724
DdeI CTNAG 1 cut(s) 479
DpnI GATC 2 cut(s) 50, 707
DpnII GATC 2 cut(s) 48, 705
DriI GACNNNNNGTC 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 70, 191
Eam1105I GACNNNNNGTC 1 cut(s) 97
EarI CTCTTC 2 cut(s) 70, 191
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 126, 741, 762
Eco47I GGWCC 2 cut(s) 387, 767
EcoO109I RGGNCCY 1 cut(s) 767
EcoT14I CCWWGG 3 cut(s) 126, 741, 762
EcoT22I ATGCAT 1 cut(s) 397
ErhI CCWWGG 3 cut(s) 126, 741, 762
FaiI YATR 6 cut(s) 250, 301, 507, 560, 623, 660
FauI CCCGC 1 cut(s) 507
Fnu4HI GCNGC 4 cut(s) 111, 223, 226, 638
FokI GGATG 1 cut(s) 382
Fsp4HI GCNGC 4 cut(s) 111, 223, 226, 638
GlaI GCGC 2 cut(s) 153, 155
GluI GCNGC 4 cut(s) 111, 223, 226, 638
HapII CCGG 1 cut(s) 285
HhaI GCGC 2 cut(s) 154, 156
Hin6I GCGC 2 cut(s) 152, 154
HinP1I GCGC 2 cut(s) 152, 154
HinfI GANTC 4 cut(s) 32, 80, 158, 574
HpaII CCGG 1 cut(s) 285
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 4 cut(s) 79, 85, 480, 609
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 6 cut(s) 73, 76, 79, 89, 92, 151
HpyAV CCTTC 4 cut(s) 535, 691, 769, 781
HpyCH4III ACNGT 2 cut(s) 295, 457
HpyCH4V TGCA 4 cut(s) 272, 395, 524, 640
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 646
HpyF3I CTNAG 1 cut(s) 479
HspAI GCGC 2 cut(s) 152, 154
Kzo9I GATC 2 cut(s) 48, 705
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 1 cut(s) 7
Lsp1109I GCAGC 2 cut(s) 97, 624
LweI GCATC 4 cut(s) 404, 533, 673, 727
MalI GATC 2 cut(s) 50, 707
MboI GATC 2 cut(s) 48, 705
MboII GAAGA 6 cut(s) 57, 146, 178, 235, 463, 793
MflI RGATCY 1 cut(s) 48
MluCI AATT 2 cut(s) 441, 483
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 3 cut(s) 57, 108, 423
MnlI CCTC 3 cut(s) 246, 481, 560
Mph1103I ATGCAT 1 cut(s) 397
MroXI GAANNNNTTC 2 cut(s) 545, 596
MslI CAYNNNNRTG 1 cut(s) 341
MspI CCGG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 545
MvnI CGCG 2 cut(s) 154, 156
MwoI GCNNNNNNNGC 2 cut(s) 151, 646
NdeII GATC 2 cut(s) 48, 705
NlaIV GGNNCC 3 cut(s) 9, 289, 768
NsiI ATGCAT 1 cut(s) 397
OliI CACNNNNGTG 1 cut(s) 341
PauI GCGCGC 1 cut(s) 152
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 1 cut(s) 545
PdmI GAANNNNTTC 2 cut(s) 545, 596
PfeI GAWTC 3 cut(s) 32, 158, 574
PkrI GCNGC 4 cut(s) 112, 224, 227, 639
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
PpuMI RGGWCCY 1 cut(s) 767
Psp5II RGGWCCY 1 cut(s) 767
PspN4I GGNNCC 3 cut(s) 9, 289, 768
PspPI GGNCC 2 cut(s) 387, 767
PspPPI RGGWCCY 1 cut(s) 767
PsuI RGATCY 1 cut(s) 48
PteI GCGCGC 1 cut(s) 152
RsaI GTAC 1 cut(s) 725
RsaNI GTAC 1 cut(s) 724
RseI CAYNNNNRTG 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 191
SatI GCNGC 4 cut(s) 111, 223, 226, 638
Sau3AI GATC 2 cut(s) 48, 705
Sau96I GGNCC 2 cut(s) 387, 767
SchI GAGTC 1 cut(s) 74
SetI ASST 9 cut(s) 132, 216, 235, 384, 438, 468, 565, 614, 747
SfaNI GCATC 4 cut(s) 404, 533, 673, 727
SinI GGWCC 2 cut(s) 387, 767
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 2 cut(s) 441, 483
SsiI CCGC 4 cut(s) 5, 222, 225, 514
StyI CCWWGG 3 cut(s) 126, 741, 762
TaaI ACNGT 2 cut(s) 295, 457
TaqI TCGA 2 cut(s) 321, 708
TasI AATT 2 cut(s) 441, 483
TauI GCSGC 2 cut(s) 225, 228
TfiI GAWTC 3 cut(s) 32, 158, 574
TscAI CASTG 3 cut(s) 298, 651, 735
TseI GCWGC 2 cut(s) 110, 637
TspGWI ACGGA 2 cut(s) 106, 151
TspRI CASTG 3 cut(s) 298, 651, 735
VpaK11BI GGWCC 2 cut(s) 387, 767
XapI RAATTY 1 cut(s) 441
XmnI GAANNNNTTC 2 cut(s) 545, 596
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.